An automatic calibration system for a water level indicator of a high-head lock comprises a PLC, an industrial control upper computer and nine water level indicators, wherein the nine water level indicators are arranged at four water level points and are respectively a first water level indicator, a second level indicator, a third water level indicator, a fourth water level indicator, a fifth water level indicator, a sixth water level indicator, a seventh water level indicator, a eight water level indicator and a ninth water level indicator, the first water level indicator, the second water level indicator and the third water level indicator are arranged outside an A gate or a base point, the fourth water level indicator and the fifth water level indicator are arranged at an A side of a gate chamber, the sixth water level indicator and the seventh water level indicator are arranged at a B side of the gate chamber, the eighth water level indicator and the ninth water level indicator arearranged outside a B gate, and the water level indicators are all connected to the PLC. Sensors of the water level indicators are used for transmitting instantaneous water level data to the PLC, andthe PLC controller is used for processing the data, calculating a correction value of each water level point and calibrating the water level indicators by changing zero-point parameters of the water level indicators. By the system, the reading accuracy and reliability of lock water level indicators is greatly improved, the purposes of running safety and transport efficiency of lock equipment system are achieved, and an idea and a direction are provided for research on automatic control of the water level indicator in the lock industry.