32 results about "Bacteria Present" patented technology

A device (1) comprising an optical apparatus (2) for monitoring bacterial growth of a drug-dosed liquid biological sample. A sample container port for receiving a sample container (6), in use, is provided in the device, the sample container (6) having at least one detection chamber (20) for containing the drug-dosed sample. The optical apparatus (2) comprises a light source (22) configured to emit light along an incident beam axis that, in use, intersects with at least one detection chamber (20) of the sample container (6), and to illuminate the drug-dosed sample contained within the detection chamber (20). The optical apparatus (20) comprises a first photodetector (26) configured to receive light scattered by bacteria in the sample. The optical apparatus (2) comprises a light collection arrangement (24) configured to collect light exiting the detection chamber (20) that has been scattered in a forward direction by bacteria in the sample, in a range of scattering angles between about + / −4 and + / −20 degrees relative to the incident beam axis, and to direct the collected scattered light to the first photodetector (26); and prevent non-scattered light travelling parallel to the incident beam axis and exiting the detection chamber (20) from reaching the first photodetector (26). The optical apparatus (2) comprises at least one processor configured to: measure an intensity of the scattered light received by the first photodetector (26); determine a corresponding representative amount or concentration of bacteria present in the sample based on the intensity of the scattered light; repeat the measuring and determining steps at a series of pre-determined intervals to determine changes in the representative amount or concentration of bacteria present in the sample as a function of time; and determine a corresponding susceptibility of the bacteria in the sample to the respective drug.

There is provided a sample cartridge for processing a fluid sample, the sample cartridge comprising: a fluid pathway having an upstream end and a downstream end, wherein the fluid pathway is adapted to receive the fluid sample at the upstream end and route the fluid sample to the downstream end; and a sample chamber fluidically connected to the downstream end of the fluid pathway, the sample chamber comprising: a chamber wall at least partially defining an internal cavity of the sample chamber, the internal cavity being adapted to receive the fluid sample from the fluid pathway, wherein the chamber wall comprises a recess; and a dehydrated bacterial growth platform for encouraging bacterial growth of bacteria present in the fluid sample, wherein the dehydrated bacterial growth platform is provided in the recess of the chamber wall.

A device (1) comprising an optical apparatus (2) for monitoring bacterial growth of a drug-dosed liquid biological sample. A sample container port for receiving a sample container (6), in use, is provided in the device, the sample container (6) having at least one detection chamber (20) for containing the drug-dosed sample. The optical apparatus (2) comprises a light source (22) configured to emit light along an incident beam axis that, in use, intersects with at least one detection chamber (20) of the sample container (6), and to illuminate the drug-dosed sample contained within the detection chamber (20). The optical apparatus (20) comprises a first photodetector (26) configured to receive light scattered by bacteria in the sample. The optical apparatus (2) comprises a light collection arrangement (24) configured to collect light exiting the detection chamber (20) that has been scattered in a forward direction by bacteria in the sample, in a range of scattering angles between about + / -4 and + / -20 degrees relative to the incident beam axis, and to direct the collected scattered light to the first photodetector (26); and prevent non-scattered light travelling parallel to the incident beam axis and exiting the detection chamber (20) from reaching the first photodetector (26). The optical apparatus (2) comprises at least one processor configured to: measure an intensity of the scattered light received by the first photodetector (26); determine a corresponding representative amount or concentration of bacteria present in the sample based on the intensity of the scattered light; repeat the measuring and determining steps at a series of pre-determined intervals to determine changes in the representative amount or concentration of bacteria present in the sample as a function of time; and determine a corresponding susceptibility of the bacteria in the sample to the respective drug.

A method for detecting bacterial nucleic acid is provided. The method includes steps of providing an extended gate field-effect transistor (EGFET) having a sensing area; immobilizing a nucleic acid probe on the sensing area, wherein the nucleic acid probe is specific to Gram-negative bacteria or Gram-positive bacteria; providing a control sample on the sensing area of the EGFET; detecting a first electrical signal of the EGFET after the control sample is provided on the sensing area of the EGFET; providing a sample on the sensing area of the EGFET; detecting a second electrical signal of the EGFET after the sample is provided on the sensing area of the EGFET; and comparing the first electrical signal and the second electrical signal to determine whether there is the bacterial nucleic acid in the sample.

To measure characteristics of a sample, and in particular, to more accurately evaluate optical characteristics of a biological sample containing microorganism particles such as bacteria.SOLUTION: An apparatus comprising an optical instrument (2) for monitoring bacterial growth in a liquid biological sample to which a drug has been administered. The optical instrument 2 comprises a light source 22 that, in use, illuminates the sample along an incident beam axis that intersects at least one of the sample containers, and a light collection arrangement 24 configured to collect light that has been scattered in a forward direction by bacteria in the sample and to direct the collected scattered light to a first photodetector 26, and to prevent unscattered light travelling parallel to the incident beam axis from reaching the first photodetector 26. Based on the intensity of the scattered light received by the first photodetector 26, a representative amount or change in concentration of bacteria present in the sample as a function of time is determined, and a corresponding susceptibility of the bacteria in the sample to the respective drug is determined.SELECTED DRAWING: Figure 2

When bacteria with different bacteriolysis targets are detected, it was necessary to lyse the individual bacteria separately with lysis solutions containing lysis enzymes suitable for each bacteriolysis target, resulting in a complicated and delayed bacteriolysis process. The purpose of this invention is to provide a new bacteriolysis method for lysing bacteria belonging to two or more species in a sample, making the bacteriolysis process more efficient and rapid.Provided is method for lysing a group of bacteria in the sample, comprising the step of lysing the group of bacteria in the sample in a mixture solution obtained by mixing the sample, a bacteriolysis enzyme, and a bacteriolysis aid, wherein the bacteriolysis enzyme includes at least one bacteriolysis enzyme selected from the group consisting of lysostaphin, lysozyme, acetyl glucosaminidase, and endopeptidase, and wherein the mixture solution has a pH of from 6.0 to 7.0 and an electrical conductivity of from 2.5 to 8.5 mS / cm, whereby bacteriolysis of two or more species of bacteria becomes possible.

A diagnostic kit and method for detecting urinary tract infections (UTIs) and bacteremia using a drinking water test in low-resource settings is provided. The kit includes a first bag containing a first culture medium capable of detecting Gram-negative and coliform bacteria through colorimetric and fluorescence indicators, and a second bag containing a second culture medium capable of detecting Gram-positive bacteria through colorimetric indicators. A method of using the kit involves inoculating a sample into each bag, incubating the bags, and observing for color changes and fluorescence to indicate bacterial presence. The kit enables rapid, cost-effective detection of bacterial pathogens in urine and blood samples without specialized laboratory equipment, facilitating early diagnosis and treatment in resource-limited environments.

The present invention provides a method for rapidly detecting bacterial, fungal and mycoplasma contamination during the culture of mesenchymal stem cells based on fluorescent quantitative PCR, and belongs to the technical field of microbial detection. A DNA-specific fragment for detecting bacteria, fungi and mycoplasma includes a DNA fragment with a nucleotide sequence such as that shown in SEQ ID NO:1 or a DNA fragment with a length of more than 50% cut off on the basis of SEQ ID NO:1. Based on the DNA-specific fragment, the present invention designs a universal primer for detecting bacteria, fungi and mycoplasma. qPCR detection using the universal primer can rapidly and specifically detect whether there is bacterial, fungal and mycoplasma contamination in mesenchymal stem cell preparations, and the method is simple and accurate, takes less time than conventional culture methods, and the results can be qualitatively analyzed.

To provide a composition and method for inhibiting bacterial flagellar motility.SOLUTION: The present invention provides an agent for inhibiting bacterial flagellar motility that contains rhatannin as an active ingredient, and a pharmaceutical composition that contains the same, as well as a method for inhibiting bacterial flagellar motility which includes contacting bacteria present in an ex vivo environment with rhatannin.SELECTED DRAWING: Figure 1

The utility model belongs to the technical field of test tube fixing frames, particularly relates to a test tube fixing frame for a clinical laboratory, and aims to solve the problems that although an existing test tube is sealed by adopting a sealing plug, the test tube is still exposed outside, the hands are polluted by bacteria when medical personnel hold a test tube shell by hand, and the sealing plug is touched, so that the test tube cannot be damaged. In order to solve the problem that bacterial contamination still exists, the utility model provides the following scheme that the device comprises a base, and four universal wheels are arranged at the bottom of the base; the height adjusting mechanism is mounted at the top of the base, a supporting circular plate is mounted at the top of the height adjusting mechanism, and two push handles are mounted on the outer side of the supporting circular plate; and a test tube holder. Through cooperation of the sealing cover and the supporting circular plate, the test tube is sealed, the dustproof protection effect is achieved, the outer side of the test tube is prevented from being polluted, the taking and placing height of the test tube can be adjusted, the test tube placing base drives the annular block to rotate on the inner wall of the annular groove, the taking and placing position of the test tube can be adjusted, and use is convenient.

The present invention provides a method for sterilizing bacteria present in a biofilm, and a liquid composition used therein, which exhibits excellent bactericidal properties for bacteria present in the biofilm. [Solution] A method for killing bacteria present in a biofilm, comprising contacting the biofilm with a liquid composition containing the following components (a) and (b), and water. (a) Components: Quaternary ammonium salt having one long-chain alkyl group represented by general formula (a1) (b) Components: Compounds having an aliphatic hydrocarbon group with 8 to 18 carbon atoms, and a group selected from a sulfate ester group, a sulfonic acid group, and salts thereof.

Resistance to antibacterial agents and / or specific resistance mechanism of bacteria is predicted by processing, for each antibacterial agent of multiple antibacterial agents, an image set (1) of a plurality of images (2) of a 3D culture matrix (30) comprising the bacteria and over which a concentration gradient of the antibacterial agent is established The processing comprises defining, for each image (2), a plurality of image regions (3) along the concentration gradient, determining, for each image region (3), a parameter (4) representing a quantity of bacteria present in a region of the 3D culture matrix (30) imaged in the image region (3), and generating a data set (5) comprising the parameters (4) determined for the plurality of image regions (3) in the plurality of images (2). Any resistance and / or resistance mechanism is predicated based on the data sets (5) for the multiple antibacterial agents and a resistance and / or resistance mechanism predicting model (125). An early indication of probable antimicrobial resistance and / or presence of specific resistance mechanisms is thereby obtained. (Fig. 7)

To provide a method for treating implantable device infections. [Solution] The present invention relates to the field of phage therapy, and more particularly to providing phage-based compositions and methods for treating or preventing infections associated with implantable devices. The compositions may be administered directly to the infection site of the device, as a single dose and / or by other means as needed, potentially avoiding the need to replace the implantable device. The method may include (a) obtaining a biological sample from the subject; (b) culturing bacteria present in the biological sample; and (c) inoculating the cultured bacteria with a phage to lyse them.

The present invention relates to a method for determining the methicillin resistance properties of a strain of Staphylococcus aureus present in a biological sample, comprising the following steps: a) incubation of the biological sample containing said strain of Staphylococcus aureus for at least 15 minutes, in the presence of a beta-lactam antibiotic selected from the following group: cefoxitin and 6-APA (6-aminopenicillanic acid), b) isolation of the bacteria present in said biological sample, c) lysis of the bacteria and hydrolysis of the bacterial proteins, in order to obtain a mixture of peptides, d) analysis of this mixture of peptides by targeted mass spectrometry, the detection of at least one peptide from the PBP2a protein (SEQ ID NO. 1) or PBP2c protein (SEQ ID NO. 2) during this analysis step being indicative of the methicillin resistance of the strain of Staphylococcus aureus present in said biological sample.

The invention discloses a breathing machine anomaly detection method, which relates to the technical field of breathing machine detection, and comprises the following steps: determining whether a breathing machine to be detected is abnormal or not according to the judgment quantity corresponding to two water film states of a liquid water film and a dripping state of the breathing machine to be detected in real-time operation time and the growth coefficients corresponding to bacteria in the two water film states; acquiring real-time bacterial load in the pipeline of the respirator to be detected; judging and generating an abnormal signal according to the real-time bacterial load in the breathing machine pipeline, reflecting pipe wall deformation caused by a water film by monitoring an axial strain value of the inner wall of the breathing machine pipeline in real time, and distinguishing two states of a liquid water film and a dripping water film; the real-time bacterial load is calculated by combining the difference rule of bacterial growth in the two states, and when the real-time bacterial load exceeds a preset early warning threshold value, an abnormal signal is generated to prompt that the breathing machine pipeline has the risk of bacterial exceeding, and potential threats to the health of a patient caused by excessive increase of the real-time bacterial load in the breathing machine pipeline are prevented.

The invention belongs to the field of microbe counting, and particularly relates to a dead and viable bacteria absolute numerical analysis method based on laser confocal fluorescence space analysis. According to the present invention, the survival state of the bacterial population is evaluated by monitoring the integrity of the cell membrane through the LIVE / DEAD BACLIGHT 1 KIT reagent (LIVE / DEADBacterium Viability Kits); the kit adopts a two-color fluorescence detection technology, and can be used for detecting viable bacteria of various bacteria genus. When the cell membrane is damaged to cause bacterial death or endangered, the cell membrane and the propidium iodide present red fluorescence or orange fluorescence (co-dyeing); and viable bacteria with a complete cell membrane and the SYTO9 dye present green fluorescence. The counting method is convenient and fast, and can accurately distinguish dead and viable bacteria.

Provided are a method for sterilizing bacteria present in a biofilm, said method exhibiting excellent bactericidal activity against bacteria present in a biofilm, and a liquid composition used therefor.　The method for sterilizing bacteria present in a biofilm comprises bringing a liquid composition into contact with the biofilm, the liquid composition containing the following component (a), component (b), and water, and having a mass ratio of the content of component (b) to the content of component (a), i.e., component (b) / component (a), of 1 to 40. Component (a): a quaternary ammonium salt having one long-chain alkyl group represented by general formula (a1) Component (b): a compound having an aliphatic hydrocarbon group with 8 to 18 carbon atoms and having a group selected from a sulfate ester group, a sulfonate group, and salts thereof

Methods for reducing scale deposition are provided. An exemplary method for reducing scale in an oilfield facility includes contacting a production surface with a production fluid including a bacteria, the bacteria present in the production fluid in a concentration of at least about 20 / mL.

A device comprising an optical apparatus for monitoring bacterial growth of a drug-dosed liquid biological sample. A sample container port for receiving a sample container, in use, is provided in the device, the sample container having at least one detection chamber for containing the drug-dosed sample. The optical apparatus comprises a light source configured to emit light along an incident beam axis that, in use, intersects with at least one detection chamber of the sample container, and to illuminate the drug-dosed sample contained within the detection chamber. The optical apparatus comprises a first photodetector configured to receive light scattered by bacteria in the sample. The optical apparatus comprises a light collection arrangement configured to collect light exiting the detection chamber that has been scattered in a forward direction by bacteria in the sample, in a range of scattering angles between about + / −4 and + / −20 degrees relative to the incident beam axis, and to direct the collected scattered light to the first photodetector; and prevent non-scattered light travelling parallel to the incident beam axis and exiting the detection chamber from reaching the first photodetector. The optical apparatus comprises at least one processor configured to: measure an intensity of the scattered light received by the first photodetector; determine a corresponding representative amount or concentration of bacteria present in the sample based on the intensity of the scattered light; repeat the measuring and determining steps at a series of pre-determined intervals to determine changes in the representative amount or concentration of bacteria present in the sample as a function of time; and determine a corresponding susceptibility of the bacteria in the sample to the respective drug.

The invention relates to an application of HNL in preparation of a reagent for identifying blood flow infection, belongs to the field of precision medical treatment, and provides an application of HNL in a detection kit for diagnosing whether a patient has blood flow infection or not, and the patient is chronic hepatitis B cirrhosis. The detection kit is used for diagnosing whether bacterial blood flow infection exists or not by detecting whether the HNL content level in a blood, plasma or serum sample taken from a patient exceeds a set cut-off value or not; the bacterial blood flow infection diagnosis of chronic hepatitis B cirrhosis combined with special primary diseases is accidentally realized through the HNL, and the sensitivity of 90.6% and the specificity of 100% of the diagnosis result are in an excellent level, so that the HNL has a relatively high clinical value and a relatively high industrial prospect.

The present invention provides a method for sterilizing bacteria present in a biofilm, and a liquid composition used therein, which exhibits excellent bactericidal properties even in the coexistence of biofilm and organic matter contamination. [Solution] A method for sterilizing bacteria present in a biofilm, comprising bringing a liquid composition containing the following components (a) and (b), and water, into contact with the biofilm in the presence of organic matter contaminants. (a) Components: Quaternary ammonium salt having two long-chain alkyl groups with 8 carbon atoms, represented by general formula (a1). (b) Components: Compounds having an aliphatic hydrocarbon group with 8 to 18 carbon atoms, and a group selected from a sulfate ester group, a sulfonic acid group, and salts thereof.

A method for detecting bacterial nucleic acids is provided. The method includes the steps of: providing an extended gate field effect transistor (EGFET) having a sensing region; fixing a nucleic acid probe on the sensing area, wherein the nucleic acid probe has specificity for gram-negative bacteria or gram-positive bacteria; providing a control group sample on a sensing region of the EGFET; detecting a first electrical signal of the EGFET after the control group sample is provided onto a sensing region of the EGFET; providing a sample on a sensing region of the EGFET; detecting a second electrical signal of the EGFET after the sample is provided onto a sensing region of the EGFET; and comparing the first electric signal with the second electric signal to judge whether bacterial nucleic acid exists in the sample.

A bacteria test method according to the invention tests for the presence or absence of bacteria in a sample, and includes amplifying a conserved sequence in bacterial 23S rRNA by PCR using a primer set consisting of a primer (1) and a primer (2). Primer (1): A primer having a sequence of SEQ ID NO: 2, and / or a primer having a sequence in which one to three bases have been modified in the sequence of SEQ ID NO: 2. Primer (2): At least one selected from the group consisting of primers having a sequence of SEQ ID NO: 3, primers having the sequence of SEQ ID NO: 3 in which one to three bases have been modified, primers having a sequence of SEQ ID NO: 36, primers having the sequence of SEQ ID NO: 36 in which one to three bases have been modified, primers having a sequence of SEQ ID NO: 39, primers having the sequence of SEQ ID NO: 39 in which one to three bases have been modified, primers having a sequence of SEQ ID NO: 41, and primers having the sequence of SEQ ID NO: 41 in which one to three bases have been modified.

The present application relates to a primer set, a kit and its application for detecting intraocular bacterial infection, and belongs to the field of biological detection technology. The primer set for detecting intraocular bacterial infection in the present application includes a detection primer pair, a detection probe, an exogenous internal standard primer pair and an exogenous internal standard probe. The present application also discloses a kit for detecting intraocular bacterial infection, including the above-mentioned primer set for detecting intraocular bacterial infection, a PCR reaction premix, a negative quality control product, a positive quality control product, a nucleic acid releaser, a PCR decontamination reagent and an exogenous internal standard. The present application can specifically detect whether the sample contains bacteria by performing qPCR on the eye (intraocular fluid) sample, and can still complete the detection of the sample when the sample amount is small or the bacterial content is low. The minimum detection limit can reach 34CFU / mL, which has the advantages of rapid and simple detection of whether bacteria exist in a single sample, and high sensitivity, strong specificity and a wide detection range.

A method of analyzing biological data is disclosed. The method comprises obtaining biological data containing at least an expression level of MX dynamin-like GTPase 1 (MX1) and an expression level of C-reactive protein (CRP) in the blood of a subject, calculating a distance between a segment of a curved line and an axis defined by a direction, the distance being calculated at a point over the curved line defined by a coordinate along the direction, and correlating the distance to the presence of, absence of, or likelihood that the subject has, a bacterial infection.

An automated system that determines the presence of bacteria in a urine sample, concentration of the bacteria, and the bacteria's resistance to a panel of antibiotics, in around one hour instead of days. An automated assay to detect cell viability in mammalian cell cultures using reduction of alamarBlue through oxidation by NADH to NAD+. The assay is conducted within a fluidic chip, wherein a sample is mixed with alamarBlue, a weakly fluorescent indicator dye. Its rate of conversion to a highly fluorescent compound is monitored using a kinetics fluorimeter for 15 minutes, and metabolic activity is shown by an increase in fluorescence. A sample with live bacteria displays a consistent and rapid increase in fluorescence, whereas the negative control exhibits little to no increase over time.

This utility model discloses a toilet that isolates bacteria, including a toilet body and a lid. The toilet body has an opening at the top, an arc-shaped non-slip handle on one side, and a water outlet 2-3 cm below the opening on the other side. A pull rope is connected to the top of the lid. This toilet reduces contamination of the bucket opening when emptying urine through the specifically positioned water outlet, and isolates bacteria using the lid with a pull rope and sealing strip, avoiding direct hand contact with the lid. It effectively solves the problems of easy contamination of the bucket opening, bacterial risks during use, and unhygienic lid use in traditional round-style toilets, significantly improving the hygiene and convenience of the toilet.

A device (1) comprising an optical apparatus (2) for monitoring bacterial growth of a drug-dosed liquid biological sample. A sample container port for receiving a sample container (6), in use, is provided in the device, the sample container (6) having at least one detection chamber (20) for containing the drug-dosed sample. The optical apparatus (2) comprises a light source (22) configured to emit light along an incident beam axis that, in use, intersects with at least one detection chamber (20) of the sample container (6), and to illuminate the drug-dosed sample contained within the detection chamber (20). The optical apparatus (20) comprises a first photodetector (26) configured to receive light scattered by bacteria in the sample. The optical apparatus (2) comprises a light collection arrangement (24) configured to collect light exiting the detection chamber (20) that has been scattered in a forward direction by bacteria in the sample, in a range of scattering angles between about + / -4 and + / -20 degrees relative to the incident beam axis, and to direct the collected scattered light to the first photodetector (26); and prevent non-scattered light travelling parallel to the incident beam axis and exiting the detection chamber (20) from reaching the first photodetector (26). The optical apparatus (2) comprises at least one processor configured to: measure an intensity of the scattered light received by the first photodetector (26); determine a corresponding representative amount or concentration of bacteria present in the sample based on the intensity of the scattered light; repeat the measuring and determining steps at a series of predetermined intervals to determine changes in the representative amount or concentration of bacteria present in the sample as a function of time; and determine a corresponding susceptibility of the bacteria in the sample to the respective drug.

Disclosed herein are methods and systems for evaluating an aspirated fluid for the presence of extracellular bacteria. The method generally includes receiving a sample cartridge comprising a sample mixture, the sample mixture comprising a liquid biological sample from a subject and a sterilized diluent, in an interior volume of the sample cartridge; imaging the sample mixture with a microscopy system; determining whether bacteria present within the sample mixture is above a configurable threshold; and in response to determining that the bacteria within the sample mixture is above the configurable threshold, determining that the liquid biological sample has extracellular bacteria.

This disclosure provides a water-using device and its cleaning control method, intelligent appliance, medium, and product. The method includes: acquiring the inlet and outlet water temperatures of the water-using device; determining that bacterial contamination exists in the water-using device when the outlet water temperature is higher than the inlet water temperature for a duration greater than or equal to a preset duration, and / or when the difference between the outlet water temperature and the inlet water temperature is greater than a first temperature threshold; and controlling the water-using device to perform sterilization and cleaning operations. By judging whether the water-using device is contaminated by bacteria based on the difference between the inlet and outlet water temperatures, the problem of misjudgment is solved from the perspective of thermodynamic characteristics. At the same time, the sterilization and cleaning operation can effectively eliminate bacteria present in the water-using device and ensure water safety.