Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
103 results about "Coliform bacteria" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Coliform bacteria are defined as rod-shaped Gram-negative non-spore forming and motile or non-motile bacteria which can ferment lactose with the production of acid and gas when incubated at 35–37°C. Due to the limited ability of certain coliform bacteria to ferment lactose, the definition has changed to bacteria containing the enzyme B-galactosidase. They are a commonly used indicator of sanitary quality of foods and water. Coliforms can be found in the aquatic environment, in soil and on vegetation; they are universally present in large numbers in the feces of warm-blooded animals. While coliforms themselves are not normally causes of serious illness, they are easy to culture, and their presence is used to indicate that other pathogenic organisms of fecal origin may be present. Such pathogens include disease-causing bacteria, viruses, or protozoa and many multicellular parasites. Coliform procedures are performed in aerobic or anaerobic conditions.
Method for feeding dsRNA restraint insect gene expression
InactiveCN101343637ALow costSimple methodBacteriaMicrobiological testing/measurementEnzyme GeneBacteria coliforms
The invention provides a method for suppressing the gene expression of insects through feeding dsRNA, which belongs to the growth development field of adjusting and controlling insects through naturally feeding the dsRNA of the silenced and specific gene. The method selects a gene fragment SeA3 on chitin synthetase enzyme gene SeCHS-A, and studies the impact on the insects after the natural feeding. The method mainly comprises the steps: a proper expression vector L4440 is selected to construct a recombinant expression vector L4440-SeA3 with a purpose gene fragment SeA3; the recombinant vector is induced and expressed in specific recipient cell coliform bacteria HT115 (DE3) under vitro adaptive condition; and the recipient bacterium htLSA3 which expresses dsRNA is added into the feedstuff of the insects, the insect larvae are naturally fed with the feedstuff, the situation of the insects suppressing the gene expression is detected after the feeding, and the change of the phenotype of the insects is recorded. By adopting natural feeding to suppress the expression of the insect genes, the method explores new paths for controlling the insects.
Owner:SUN YAT SEN UNIV
Method for producing chlorella fermented food
InactiveUS7914832B2Production is suppressedReduce flavorTea extractionAnimal feeding stuffBiotechnologyLactobacillus
Owner:KYOTO EIYO +1
Animal Bifidobacterium and use method thereof
ActiveCN102191192AGood synergistic growthGood recombinationMilk preparationBacteriaEscherichia coliMicrobiological culture
The invention relates to an animal Bifidobacterium and a use method thereof. The animal Bifidobacterium is the Bifidobactrium amimalis Subsp Lactis B174, the CGMCC No. is 4182, the conservation date is September 16, 2010 and the conservation unit is the China General Microbiological Culture Collection Center. The Bifidobactrium amimalis Subsp Lactis B174 can colonize in human body and resist the gastric acid and cholate of human body. Meanwhile, the strain has better inhibitory action on Escherichia coli and can survive for 30 days at the room temperature. The strain and other lactic acid bacteria have better synergic action for growth; the strain has better combination effect with lactic acid bacteria; the product has better flavor, can be used to prepare fermented yoghurt, animal feed additives and antibacterial solution and can be widely used in the fields of other foods and biological products.
Owner:BEIJING BOJINYUAN BIOTECH
Electrochemiluminescent assays
Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety. Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate. The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.
Owner:BIOVERIS CORP
Test chip for fast detecting microorganism, preparation and use thereof
InactiveCN101497914AAvoid missingEasy to operateMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementFood borneColony count
The invention discloses a testing piece for quickly testing microorganisms, as well as a preparation method and application thereof. The testing piece comprises a soleplate, a film with a hollow part in the middle and a plastic film. The bottom surface of the film with the hollow part in the middle is adhered to the soleplate; a groove culture area is formed at the hollow part in the middle of the film; a color development culture medium is arranged in the groove culture area; a cold water condensable gellant is adhered to the bottom surface of the plastic film; and the bottom surface of the plastic film and the upper surface of the film are adhered to the edge at one side. After the upper and the lower layers of the testing piece are adhered, irradiation and sterilization packages are used for standby. The testing piece can be applied to the detection of total colony count, coliform bacteria, coliform group, salmonella, staphylococcus aureus, subsidiary haemolytic vibrio, pseudomonas aeruginosa and other food-borne causative organisms and has the advantages of simple operation, convenient carrying, short testing time, accurate result and double-sided bacteria count.
Owner:GUANGDONG INST OF MICROORGANISM
Method for specific fast detection of relevant bacteria in drinking water
InactiveUS20050064444A1Sugar derivativesMicrobiological testing/measurementBiotechnologyEscherichia coli
The invention relates to a method for detecting bacteria in drinking water and surface water, especially a method for simultaneous specific detection of bacteria from the Legionella species and the Legionella pneumophila species by in situ hybridization. The invention also relates to a method for specific detection of faecal streptococci by in situ-hybridization and a method for simultaneous specific detection of coliform bacteria and bacteria of the Escherichia coli species, in addition to corresponding oligonucleotide probes and kits enabling said inventive method to be carried out.
Owner:VERMICON
Antibacterial chitosan gel and preparation method thereof
ActiveCN104027300ALower resistanceHigh biosecurityOrganic active ingredientsPeptide/protein ingredientsCarrageenanIrritation
The invention relates to antibacterial chitosan gel and a preparation method of the antibacterial chitosan gel. The preparation method includes the steps of preparing complex liquid containing chitosan, preparing a fibroin ethanol solution, preparing a carrageenan solution, preparing a quaternary phosphonium compound solution, preparing gel and other steps. According to the antibacterial chitosan gel, high-molecular-weight chitosan and low-molecular-weight chitosan compounds are used as main base materials in cooperation with antibiotic synergist, gelatinizing agents, histocyte repairing auxiliary materials and the like for being compounded, and use of other chemicals, biological drugs and other ingredients is avoided; as a result, the safety of the gel is improved, cost is reduced, irritation to patients is reduced, and health restoration of the patients is facilitated. The ability of the prepared antibacterial chitosan gel to resist bacteria such as staphylococcus aureus, coliform bacteria, streptococcus and pseudomonas aeruginosa reaches more than 99 percent, so the antibacterial chitosan gel shows good bacteriostatic ability.
Owner:JIANGSU RUIJING TECH DEV
Fusion protein of tetanus toxin T cell expressing bit polypeptide and human bata lymph cell stimulating factor and preparing thereof
InactiveCN1793178AImprove protectionOvercome the limitations of self-toleranceHybrid peptidesVector-based foreign material introductionEscherichia coliDisease
The invention discloses tetanus toxin T cell epi position peptide and human B lymphocyte activate and gene interfusing albumen. It could activate CD4+T cell to take cell action, promote body liquid immunity level. It adopts step clone to construct TT and BLyS interfusing gene and gain high efficiency expression in coliform bacteria. It conquers the immunity tolerate of the body.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY
Food and tableware coliform bacteria rapid detection scrip preparation and application
InactiveCN104611403AAccurate detectionReduce the impact of interferenceMicrobiological testing/measurementBiotechnologyMicroorganism
The invention provides a coliform bacteria rapid detection cold water gel scrip, and the detection cold water gel scrip is a hygiene inspection product special for rapid detection of coliform bacteria number in food and tableware. Through reasonable ratio of culture medium components and cold water gel components, scientific production process and the appropriate packaging, the product cost is low, the operation method is simple and easy to operate, detection procedure is simple and fast, so that cumbersome tube fermentation culture and physical and chemical test are simplified and can be completed in one step, sensitivity is good, specificity is strong, results are accurate, the national standard method coincidence rate is high; meanwhile antibacterial substances are added into the culture medium to reduce the interference of other microorganisms. The rapid detection scrip can achieve quick and accurate detection and qualitative and quantitative analysis of coliform bacteria, and can be applied to the rapid detection of the coliform bacteria in food and tableware.
Owner:HUBEI UNIV OF TECH
Vibrio parahaemolyticus tunica externa protein ompK subunit vaccine and preparation method thereof
InactiveCN101172157APreserve immunogenicityDefense against invasionAntibacterial agentsDigestive systemEscherichia coliHaemolysis
The invention discloses protein ompK subunit vaccine of assistant haemolysis vibrio extine and a preparation method thereof. The vaccine is PBS solution which converts the recombination protein of the coliform bacteria of recombination prokaryon expression plasmid pET30a-ompK expressed by inducing and after being purified; the concentration of the PBS solution is 0.25-0.5mg / ml. The method has the steps that: firstly, the extraction of an assistant haemolysis vibrio full gene group, the overall length of extine protein ompK DNA and the clone of a mature peptide coded sequence; secondly, the construction of a prokaryon expression plasmid of the ompK mature peptide coded sequence, thirdly, the obtaining way of the recombination ompK protein; fourthly, the detection to the immunity way and the immunity effect of a large yellow croaker with recombination ompK protein. The invention provides the preparation method of the assistant haemolysis vibrio ompK protein subunit vaccine, and simultaneously provides the detection method of the immunity effect of the large yellow croaker with recombination ompK protein, the preparation method is simple, and the usage is convenient.
Owner:ZHEJIANG UNIV
Sanitary material
InactiveCN101612416AImprove comfortGood hygroscopicityAbsorbent padsNon-woven fabricsTriclosanDry weight
The invention relates to a sanitary material. The sanitary material is silk water-jetting non-woven fabric which uses silk short fibers as raw materials and is prepared by the water-jetting technology. wherein in the after-finishing process of the silk water-jetting non-woven fabric, an organic antibacterial agent which contains at least one of double-chain quaternary ammonium salt, biguanides and triclosan is coated on the silk water-jetting non-woven fabric by the on-line coating technology, wherein the dry weight ratio of the consumption of the antibacterial agent to that of the silk water-jetting non-woven fabric is 0.5:1000-5:1000. The silk water-jetting non-woven fabric has the advantages of good comfort property, fineness, smoothness, lightness, softness, good hygroscopic property and ventilation property and refined pearly luster and good safety and stability; in addition ,the 20-minute antibacterial ratio of the silk water-jetting non-woven fabric against bacteria, such as coliform bacteria and candida albicans, is larger than 90-95 percent, has quick, durable, high-efficiency and broad-spectrum antibacterial effects, and has no irritation, no allergic reaction and no corrosion.
Owner:FUJIAN HENGAN HLDG CO LTD +2
Prediction method for growth of putrefying bacteria in modified atmosphere packaged fresh chilled beef
InactiveCN102676634APredicted bacterial loadInput/output for user-computer interactionMicrobiological testing/measurementNODALBacteroides
The invention discloses a prediction method for growth of putrefying bacteria in modified atmosphere packaged fresh chilled beef. A BP (Back-Propagation) artificial neural network which comprises an input layer, a hidden layer and an output layer is adopted; the input layer comprises storage temperature, modified atmosphere proportion, strain and beef storage time for setting a prediction range; the strain can be selected from pseudomonas, lactic acid bacteria, brochothrix thermosphacta or coliform bacteria; the hidden layer is used for processing complex nonlinear relation between input data and output data; after optimization and comparison, the most accurate result can be obtained when 11 neuron nodes included in the hidden layer is determined; the output layer is the bacteria number of the putrefying bacteria in the required beef at a certain moment under a specific storage condition; adopted activation functions between layers are a hyperbolic tangent propagation function and a linear propagation function respectively; and training and learning are performed on the constructed BP artificial neural network by using data obtained by experiments, so that the bacteria numbers of the putrefying bacteria in the beef at different moments under different storage conditions are predicted.
Owner:SHANGHAI OCEAN UNIV
Coliform detection process and kit for use therein
InactiveUS20110269179A1Improve sampling efficiencyClogging problemPollution detectorsMicrobiological testing/measurementLarge intestineCulture mediums
A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.
Owner:3M INNOVATIVE PROPERTIES CO
Electrochemiluminescent assays
Owner:BIOVERIS CORP
Manufacture method of fresh-keeping garlic rice
InactiveCN101849571AOvercome the disadvantages of hot blanchingGuarantee the inner qualityFruits/vegetable preservation by freezing/coolingEscherichia coliBacteria coliforms
The invention discloses a manufacture method of fresh-keeping garlic rice, which has the main technical scheme that garlic is manually divided into cloves and is peeled, the peel of the garlic is removed, then, the garlic is soaked for 10 minutes in color protecting and sterilization liquid, and the garlic carries out vacuum packing and refrigeration. The invention has the advantages of simple production process and fresh garlic rice. The total number of colonies of 1g sample bacteria is smaller than 10000, and the coliform bacteria of 100g sample feces is less than 3. The bacillus cereuses are less than 3 / g, and the shelf life is 90 days.
Owner:徐州建华食品有限公司
DNA chip, kit for detecting or genotyping bacteria causing sexually transmitted diseases, genotyping antibacterial drug resistance and detecting or genotyping method using the same
InactiveUS20120004113A1Quickly and accurately detectEasy to explainNucleotide librariesMicrobiological testing/measurementDiseaseEscherichia coli
Disclosed are a DNA chip and a kit capable of quickly and accurately detecting or genotyping the highly prevalent and important eleven microbes causing sexually transmitted diseases (STD) Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, syphilis-causing treponema, pallidum, chancroid-causing Haemophilus ducreyi, genital herpes-causing herpes simplex virus 1 and 2, human papillomavirus (HPV) and Trichomonas vaginalis and three related organisms Candida albicans, Gardnerella vaginalis and coliform bacteria and analyzing antibiotic resistance against tetracycline and lactam antibiotics, and a method for detecting or genotyping using the same. According to the present invention, the presence, genotype and antibiotic resistance of the fourteen organisms can be analyzed quickly and accurately from a DNA sample. With excellent sensitivity, specificity, reproducibility and accuracy of the 14 STD-causing and related microorganisms may be automatically identified quickly and accurately from multiple samples, and selection of antibiotics may be aided.
Owner:GOODGENE
Method for producing chlorella fermented food
An object of the present invention is to provide a method for producing a chlorella fermented food in which a flavor and odor that are peculiar to algae are reduced while production of pheophorbide is suppressed. The method for producing a chlorella fermented food according to the present invention can be achieved by using a chlorella negative in coliform bacteria count, and having a content of free pheophorbide equal to or less than 18 mg % and a viable general bacteria count equal to or less than 8000 cfu / mL, or using a chlorella subjected to a heat sterilization treatment to ferment the chlorella with a baker's yeast. The baker's yeast is blended with the chlorella at a ratio of preferably 0.1 to 15% by weight. Further, the fermentation treatment is preferably carried out in the presence of glucose and a lactic acid bacterium.
Owner:KYOTO EIYO +1
Method and apparatus for detecting coliform bacteria from reflected light
InactiveUS20050164332A1Easy to holdMicrobiological testing/measurementTesting waterBacteria coliformsBiology
The present invention relates to a method of detecting coliform bacteria in water from reflected light, and also includes devices for the measurement, calculation and transmission of data relating to that method.
Owner:BOWLING GREEN STATE UNIV
Method for preparing positive control for single-tube RT-PCR detection of three fish disease virus
InactiveCN1775954APositive Control SafetyEasy to storeMicrobiological testing/measurementFermentationEscherichia coliPositive control
The invention relates to a method of make single valve RT-PCR dectecting masculine comparing virulence haemorrhagic septicemia virus, fish infectivity hemocytopoietic organ putrescence and infectivity pancreas putrescence germina. Its feature is selecting G gene from virulence haemorrhagic septicemia virus, VP gene from fish infectivity hemocytopoietic organ putrescence and N gene from infectivity pancreas putrescence germina, inserting into pUC57 particle carrier and transfect to DH5 alpha coliform bacteria to take in vitro expression, and extending the section 625bp, 371bp, 206bp. It has very high use value.
Owner:LIANYUN PORT IMMIGRATION INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
Recombinant porcine interferon beta1-Fc fusion protein as well as encoding gene and expressing method thereof
ActiveCN103570836AMaintain biological activityExtended half-lifeBacteriaMicroorganism based processesEscherichia coliInclusion bodies
The invention provides a recombinant porcine interferon IFN beta1-Fc fusion protein as well as an encoding gene and expression, purification and inclusion body renaturation methods thereof, belonging to the biological genetic engineering field. The IFNbeta can enhance the immunity of pig and has a good application prospect in the veterinary medicine industry. However, natural porcine IFNbeta1 is less in expression quantity and is insufficient for research and development and application and has the deficiency of quick plasma clearance rate. The invention provides the recombinant porcine interferon IFN beta1-Fc fusion protein applicable to a coliform bacteria prokaryotic expression system. Part of the porcine IFNbeta1 is all sequences in an ectoenzyme area of the porcine IFNbeta1. The Fc section comprises a hinge region, a CH2 region and a CH3 region of an antibody. The porcine IFNbeta1 and the Fc section are directly fused. The fusion protein provided by the invention not only maintains the biological activity of the original protein IFNbeta1 to a great extent, but also extremely prolongs the half-life period of the original protein IFNbeta1, thereby providing the opportunity of industrialized development of the fusion protein.
Owner:GENSUN INST OF BIOMEDICINE
Unpowered purification tank
InactiveCN103086564AEmission complianceMultistage water/sewage treatmentBacteria coliformsFilter material
The invention relates to an unpowered purification tank which comprises a tank body, a flow inlet, a flow outlet, an inspection port and a cover overlaid on the inspection port, wherein the tank body is divided into a first anaerobic tank and a second anaerobic tank through a partition plate in the middle space of the tank body; the bottom of each anaerobic tank is provided with a grate shelf for carrying a filtering material; the flow inlet is positioned above the first anaerobic tank; the flow outlet is positioned above the second anaerobic tank; and the highest point of the flow outlet is below the horizontal line of the lowest point of the flow inlet. The invention has the following advantages: 1) domestic sewage discharged from toilets, kitchens, bathrooms, lavatories, washing machines and the like can be purified without power; 2) a filtering tank has an automatic cleaning function, and foreign bodies blocking the filtering material can be cleaned away without any power; 3) through the anaerobic tanks and the filtering tank, 50-80% of BOD (biochemical oxygen demand) and 50-80% of floating substances in the domestic sewage can be removed; and 4) through a sterilizing tank, coliform bacteria in the domestic sewage can be killed, thereby achieving up-to-standard discharge.
Owner:KANSAIKAKO
Processing method of fresh and crispy freeze-dried jujubes and jujube slices
The invention discloses a processing method of fresh and crispy freeze-dried jujubes and jujube slices. By adopting acidic electrolyzed water and sterilization and enzyme deactivation treatment and freeze-drying technologies, the dried jujube products have the advantages of intact shape, bright color, high nutrient content, aromatic flavor and good taste. The total number of bacterial colonies ofthe prepared finished products are less than 1000 CFU / g, the number of coliform bacteria of the finished products is less than 30 MPN / 100g, the cross sections of the finished products show vivid verdant color, and the finished products have no band-shaped brown stripes which are usually shown in common freeze-dried products; by adjusting a freeze-drying curve, the moisture content of the productsis controlled to be 4%-10%, the products have rich fresh and aromatic flavor, and the taste of the products is similar to the taste of fresh jujubes; the finished products have crispy taste similar tothe taste of vacuum puffed products and low-temperature fried products and have no greasy feeling; and nutrients are better kept, and the retention rate of vitamin C is higher than 90%.
Owner:早康枸杞股份有限公司
Bastard tamarind legume extract, and preparation method thereof and anti-bacteria agent
InactiveCN101461386AEnhanced inhibitory effectTake advantage ofBiocideDisinfectantsEscherichia coliPenicillin
The invention relates to a silk tree pod extract and preparation method thereof with antibacterial agent. The silk tree pod extract uses silk tree pod as raw materials, which is extracted in the solvent extraction through separating solvent after leaching, extract is obtained through distilling extract phase. The antibacterial agent prepared by the extract has different inhibitory action on coliform bacteria, staphylococcus aureus, salmonella, huge bacillus, bacillus subtilis and Pseudomonas aeruginosa, wherein ether extract has the strongest inhibitory action on the above fungi, particularly for the huge bacillus, bacillus subtilis with higher inhibitory action than on penicillin. The invention makes full use of silk tree pod resource which is not developed, provides operable key technology for developing novel antibacterial agent, and provides novel technical support for plant antimicrobial agent.
Owner:HUAIYIN TEACHERS COLLEGE
Antibodies for detection of colistin-resistance
ActiveUS20190322727A1High of confidenceHigh degree of sensitivityImmunoglobulins against bacteriaPolymyxinsMonoclonal antibodyColistin resistance
Monoclonal antibodies against bacterial proteins that confer resistance to the antibiotic colistin and hybridomas that produce such monoclonal antibodies are described. The monoclonal antibodies have a strong affinity for MCR proteins and may be used in detection methods and kits to detect the presence of MCR proteins including variants thereof in a sample.
Owner:UNITED STATES OF AMERICA
Water purification process for preparing living water
The invention provides a water purification process for preparing living water. The water purification process comprises the following steps carried out on the water to be treated: filtering to remove suspended solids; aeration treatment to oxidize Fe<2+> and Mn<2+> in water; active biological membrane contact oxidation and filtration treatment to remove iron and manganese; activated carbon adsorption treatment to remove organic pollutants and pesticides; nanofiltration treatment to remove heavy metal ions; and sterilization. The invention has the beneficial effect that the process can purify water source with complex components (e.g. riverbed groundwater containing coliform bacteria, turbidity, heavy metal ions and organic pollutants) into living water meeting the national standard.
Owner:PANGANG GRP ENG TECH
Test piece for quick test of coliform bacteria and preparation method and test method of test piece
InactiveCN106811404AEasy to distinguishEvenly distributedBioreactor/fermenter combinationsBiological substance pretreatmentsMicroorganismBacteria coliforms
The invention provides a test piece for quick test of coliform bacteria. The test piece comprises a lower substrate, pearl paper, an upper thin film component and a coliform bacterial characteristic chromogenic fluid medium. The middle of the pearl paper is provided with a circular groove, and the coliform bacterial characteristic chromogenic fluid medium is uniformly distributed in the circular groove. The test piece for quick test of the coliform bacteria has advantages of easiness in distinguishing of target bacteria, chromogenic clearness, simplicity in result determination, reliability in test result, short test time, simplicity and convenience in operation, low cost and the like and can play a significant role in microbiological detection.
Owner:贵州勤邦食品安全科学技术有限公司
Biological bacterial fertilizer prepared from poultry and livestock waste and preparation method thereof
ActiveCN107473867APromote growthGood for seedingBio-organic fraction processingAnimal corpse fertilisersCelluloseBiotechnology
The invention provides biological bacterial fertilizer prepared from poultry and livestock waste. The biological bacterial fertilizer meets the following indexes that the number of effective living bacteria is greater than or equal to 42 million / g; the content of N+P2O5+K2O is greater than or equal to 8%, and the content of organic matter is greater than or equal to 45%; the content of amino acids is greater than or equal to 6%; the content of humic acid is greater than or equal to 5%; the germination index of seeds is greater than or equal to 95%; the content of soluble carbon is smaller than or equal to 0.56; the number of coliform bacteria is smaller than or equal to 60 / g; the mortality of ascaris eggs is greater than or equal to 97%; the fertilizer has no pungent odor. The invention further discloses a preparation method of the biological bacterial fertilizer prepared from the poultry and livestock waste. The method comprises the step of preparing carrier materials; the prepared biological bacterial fertilizer is long in service life and especially beneficial to crop growth and seeding, and has beneficial strains, various enzymes and trace elements such as various vitamins, carbohydrate, cellulose and nucleic acid; the microbial activity of soil can be enhanced, the number of beneficial bacteria in root-layer soil is increased, and the activity of the beneficial bacteria in the root-layer soil is improved.
Owner:SHANDONG WOKEQI IND CO LTD
Use of reporter plasmid for biological detection of radioactive ray dose
InactiveCN101348823AHigh precisionReal-time monitoring of fertility statusDosimetersMicrobiological testing/measurementEscherichia coliShuttle vector
The invention relates to an application of a reporter plasmid for biological detection of radiation dose. The application comprises the following steps that a shuttle vector between radiation resistant bacteria and coliform bacteria is taken as a foundation so as to form a reporter plasmid of the shuttle vector which has luciferase gene activity and is positioned between the radiation resistant bacteria and the coliform bacteria; the reporter plasmid is transformed into a wild type R1 of radiation resistant bacteria to obtain transformant radiation resistant bacteria which are irradiated by gamma ray; and according to the luminous quantity of a character transformant, luminous quantities after irradiation by gamma rays in different doses are measured. The reporter plasmid can be used for biological detection of high radiation dose and low radiation dose, and can be widely used in the detection of nuclear facilities such as a nuclear power station, radiation irradiation facilities in a hospital, and the like, and other places with radioactive sources; moreover, the reporter plasmid can also be used in contamination detection for radioactive substances in an environment, and can induce a plurality of DNA damaging agents as a bioinductor; meanwhile, the reporter plasmid has a luciferase gene, and plays a positive role in realtime monitoring of aspects of procreation, distribution and environmental influence of a recombined transformant, and so on.
Owner:ZHEJIANG UNIV
Method for preparing rebuilding heat-resistant xylose/dextrose isomerase by employing pHsh expression system
InactiveCN101037686AEfficient expressionImprove expression levelBacteriaMicroorganism based processesEscherichia coliLevel structure
A preparing method of recombination heat resistance xylose / glucose isomerase, including: first, extracting genome DNA of thermozymes, amplificating PCR to obtain isomerase gene, inserting the gene in gene to express carrier pHsh, inverting coliform bacteria to obtain isomerase expression plasmid; second, mutagisming gene, changing usage frequency of codon and two-level structure of mRNA to obtain optimized isomerase expression plasmid; third, shifting the isomerase expression plasmid or optimized isomerase expression plasmid into coliform bacteria to obtain gene strains, and expressing xylose / glucose isomerase by thermally stimulating and inducing; finally, collecting cells, rupturing and centrifugating to obtain rough enzyme liquid, further purifying to obtain 90% purness of recombination heat resistance xylose / glucose isomerase. The invention first uses expression carrier pHsh to efficent express heat resistance xylose / glucose isomerase gene of thermozymes, producibility of recombination enzyme using this technque is more than 200 mg / L, the product has good stability and the method is easy to industrialized produce.
Owner:SHINE E BIOTECH CO LTD
Ozonating water treatment and filtration apparatus
ActiveUS9469557B1Easy to installImprove reliabilitySpecific water treatment objectivesWater contaminantsWater treatmentChemistry
An ozonating water treatment and filtration apparatus is provided wherein a groundwater containing contaminates including hydrogen sulfide, ferrous oxide, carbon dioxide, manganese, arsenic, coliform bacteria and other pathogens and compounds, are removed by filtration utilizing a zeolite and pea gravel filter media and by application of an oxidant using low concentrations of ozone. A silent ozone generator and injector feature provides silent operation and no ozone pumping mechanism. The apparatus is self regenerating, low cost, and low maintenance requiring few consumables and minimal user intervention.
Owner:ALBERIC CORP
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com