103 results about "Coliform bacteria" patented technology

The invention provides a method for suppressing the gene expression of insects through feeding dsRNA, which belongs to the growth development field of adjusting and controlling insects through naturally feeding the dsRNA of the silenced and specific gene. The method selects a gene fragment SeA3 on chitin synthetase enzyme gene SeCHS-A, and studies the impact on the insects after the natural feeding. The method mainly comprises the steps: a proper expression vector L4440 is selected to construct a recombinant expression vector L4440-SeA3 with a purpose gene fragment SeA3; the recombinant vector is induced and expressed in specific recipient cell coliform bacteria HT115 (DE3) under vitro adaptive condition; and the recipient bacterium htLSA3 which expresses dsRNA is added into the feedstuff of the insects, the insect larvae are naturally fed with the feedstuff, the situation of the insects suppressing the gene expression is detected after the feeding, and the change of the phenotype of the insects is recorded. By adopting natural feeding to suppress the expression of the insect genes, the method explores new paths for controlling the insects.

The invention relates to an animal Bifidobacterium and a use method thereof. The animal Bifidobacterium is the Bifidobactrium amimalis Subsp Lactis B174, the CGMCC No. is 4182, the conservation date is September 16, 2010 and the conservation unit is the China General Microbiological Culture Collection Center. The Bifidobactrium amimalis Subsp Lactis B174 can colonize in human body and resist the gastric acid and cholate of human body. Meanwhile, the strain has better inhibitory action on Escherichia coli and can survive for 30 days at the room temperature. The strain and other lactic acid bacteria have better synergic action for growth; the strain has better combination effect with lactic acid bacteria; the product has better flavor, can be used to prepare fermented yoghurt, animal feed additives and antibacterial solution and can be widely used in the fields of other foods and biological products.

Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety. Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate. The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.

The invention discloses a testing piece for quickly testing microorganisms, as well as a preparation method and application thereof. The testing piece comprises a soleplate, a film with a hollow part in the middle and a plastic film. The bottom surface of the film with the hollow part in the middle is adhered to the soleplate; a groove culture area is formed at the hollow part in the middle of the film; a color development culture medium is arranged in the groove culture area; a cold water condensable gellant is adhered to the bottom surface of the plastic film; and the bottom surface of the plastic film and the upper surface of the film are adhered to the edge at one side. After the upper and the lower layers of the testing piece are adhered, irradiation and sterilization packages are used for standby. The testing piece can be applied to the detection of total colony count, coliform bacteria, coliform group, salmonella, staphylococcus aureus, subsidiary haemolytic vibrio, pseudomonas aeruginosa and other food-borne causative organisms and has the advantages of simple operation, convenient carrying, short testing time, accurate result and double-sided bacteria count.

The invention relates to a method for detecting bacteria in drinking water and surface water, especially a method for simultaneous specific detection of bacteria from the Legionella species and the Legionella pneumophila species by in situ hybridization. The invention also relates to a method for specific detection of faecal streptococci by in situ-hybridization and a method for simultaneous specific detection of coliform bacteria and bacteria of the Escherichia coli species, in addition to corresponding oligonucleotide probes and kits enabling said inventive method to be carried out.

The invention discloses tetanus toxin T cell epi position peptide and human B lymphocyte activate and gene interfusing albumen. It could activate CD4⁺T cell to take cell action, promote body liquid immunity level. It adopts step clone to construct TT and BLyS interfusing gene and gain high efficiency expression in coliform bacteria. It conquers the immunity tolerate of the body.

The invention provides a coliform bacteria rapid detection cold water gel scrip, and the detection cold water gel scrip is a hygiene inspection product special for rapid detection of coliform bacteria number in food and tableware. Through reasonable ratio of culture medium components and cold water gel components, scientific production process and the appropriate packaging, the product cost is low, the operation method is simple and easy to operate, detection procedure is simple and fast, so that cumbersome tube fermentation culture and physical and chemical test are simplified and can be completed in one step, sensitivity is good, specificity is strong, results are accurate, the national standard method coincidence rate is high; meanwhile antibacterial substances are added into the culture medium to reduce the interference of other microorganisms. The rapid detection scrip can achieve quick and accurate detection and qualitative and quantitative analysis of coliform bacteria, and can be applied to the rapid detection of the coliform bacteria in food and tableware.

The invention discloses protein ompK subunit vaccine of assistant haemolysis vibrio extine and a preparation method thereof. The vaccine is PBS solution which converts the recombination protein of the coliform bacteria of recombination prokaryon expression plasmid pET30a-ompK expressed by inducing and after being purified; the concentration of the PBS solution is 0.25-0.5mg/ml. The method has the steps that: firstly, the extraction of an assistant haemolysis vibrio full gene group, the overall length of extine protein ompK DNA and the clone of a mature peptide coded sequence; secondly, the construction of a prokaryon expression plasmid of the ompK mature peptide coded sequence, thirdly, the obtaining way of the recombination ompK protein; fourthly, the detection to the immunity way and the immunity effect of a large yellow croaker with recombination ompK protein. The invention provides the preparation method of the assistant haemolysis vibrio ompK protein subunit vaccine, and simultaneously provides the detection method of the immunity effect of the large yellow croaker with recombination ompK protein, the preparation method is simple, and the usage is convenient.

A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.

The invention discloses a manufacture method of fresh-keeping garlic rice, which has the main technical scheme that garlic is manually divided into cloves and is peeled, the peel of the garlic is removed, then, the garlic is soaked for 10 minutes in color protecting and sterilization liquid, and the garlic carries out vacuum packing and refrigeration. The invention has the advantages of simple production process and fresh garlic rice. The total number of colonies of 1g sample bacteria is smaller than 10000, and the coliform bacteria of 100g sample feces is less than 3. The bacillus cereuses are less than 3/g, and the shelf life is 90 days.

Disclosed are a DNA chip and a kit capable of quickly and accurately detecting or genotyping the highly prevalent and important eleven microbes causing sexually transmitted diseases (STD) Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, syphilis-causing treponema, pallidum, chancroid-causing Haemophilus ducreyi, genital herpes-causing herpes simplex virus 1 and 2, human papillomavirus (HPV) and Trichomonas vaginalis and three related organisms Candida albicans, Gardnerella vaginalis and coliform bacteria and analyzing antibiotic resistance against tetracycline and lactam antibiotics, and a method for detecting or genotyping using the same. According to the present invention, the presence, genotype and antibiotic resistance of the fourteen organisms can be analyzed quickly and accurately from a DNA sample. With excellent sensitivity, specificity, reproducibility and accuracy of the 14 STD-causing and related microorganisms may be automatically identified quickly and accurately from multiple samples, and selection of antibiotics may be aided.

An object of the present invention is to provide a method for producing a chlorella fermented food in which a flavor and odor that are peculiar to algae are reduced while production of pheophorbide is suppressed. The method for producing a chlorella fermented food according to the present invention can be achieved by using a chlorella negative in coliform bacteria count, and having a content of free pheophorbide equal to or less than 18 mg % and a viable general bacteria count equal to or less than 8000 cfu/mL, or using a chlorella subjected to a heat sterilization treatment to ferment the chlorella with a baker's yeast. The baker's yeast is blended with the chlorella at a ratio of preferably 0.1 to 15% by weight. Further, the fermentation treatment is preferably carried out in the presence of glucose and a lactic acid bacterium.

The present invention relates to a method of detecting coliform bacteria in water from reflected light, and also includes devices for the measurement, calculation and transmission of data relating to that method.

The invention relates to a method of make single valve RT-PCR dectecting masculine comparing virulence haemorrhagic septicemia virus, fish infectivity hemocytopoietic organ putrescence and infectivity pancreas putrescence germina. Its feature is selecting G gene from virulence haemorrhagic septicemia virus, VP gene from fish infectivity hemocytopoietic organ putrescence and N gene from infectivity pancreas putrescence germina, inserting into pUC57 particle carrier and transfect to DH5 alpha coliform bacteria to take in vitro expression, and extending the section 625bp, 371bp, 206bp. It has very high use value.

The invention provides a recombinant porcine interferon IFN beta1-Fc fusion protein as well as an encoding gene and expression, purification and inclusion body renaturation methods thereof, belonging to the biological genetic engineering field. The IFNbeta can enhance the immunity of pig and has a good application prospect in the veterinary medicine industry. However, natural porcine IFNbeta1 is less in expression quantity and is insufficient for research and development and application and has the deficiency of quick plasma clearance rate. The invention provides the recombinant porcine interferon IFN beta1-Fc fusion protein applicable to a coliform bacteria prokaryotic expression system. Part of the porcine IFNbeta1 is all sequences in an ectoenzyme area of the porcine IFNbeta1. The Fc section comprises a hinge region, a CH2 region and a CH3 region of an antibody. The porcine IFNbeta1 and the Fc section are directly fused. The fusion protein provided by the invention not only maintains the biological activity of the original protein IFNbeta1 to a great extent, but also extremely prolongs the half-life period of the original protein IFNbeta1, thereby providing the opportunity of industrialized development of the fusion protein.

Monoclonal antibodies against bacterial proteins that confer resistance to the antibiotic colistin and hybridomas that produce such monoclonal antibodies are described. The monoclonal antibodies have a strong affinity for MCR proteins and may be used in detection methods and kits to detect the presence of MCR proteins including variants thereof in a sample.

The invention provides a water purification process for preparing living water. The water purification process comprises the following steps carried out on the water to be treated: filtering to remove suspended solids; aeration treatment to oxidize Fe<2+> and Mn<2+> in water; active biological membrane contact oxidation and filtration treatment to remove iron and manganese; activated carbon adsorption treatment to remove organic pollutants and pesticides; nanofiltration treatment to remove heavy metal ions; and sterilization. The invention has the beneficial effect that the process can purify water source with complex components (e.g. riverbed groundwater containing coliform bacteria, turbidity, heavy metal ions and organic pollutants) into living water meeting the national standard.

The invention provides a test piece for quick test of coliform bacteria. The test piece comprises a lower substrate, pearl paper, an upper thin film component and a coliform bacterial characteristic chromogenic fluid medium. The middle of the pearl paper is provided with a circular groove, and the coliform bacterial characteristic chromogenic fluid medium is uniformly distributed in the circular groove. The test piece for quick test of the coliform bacteria has advantages of easiness in distinguishing of target bacteria, chromogenic clearness, simplicity in result determination, reliability in test result, short test time, simplicity and convenience in operation, low cost and the like and can play a significant role in microbiological detection.

The invention provides biological bacterial fertilizer prepared from poultry and livestock waste. The biological bacterial fertilizer meets the following indexes that the number of effective living bacteria is greater than or equal to 42 million/g; the content of N+P2O5+K2O is greater than or equal to 8%, and the content of organic matter is greater than or equal to 45%; the content of amino acids is greater than or equal to 6%; the content of humic acid is greater than or equal to 5%; the germination index of seeds is greater than or equal to 95%; the content of soluble carbon is smaller than or equal to 0.56; the number of coliform bacteria is smaller than or equal to 60/g; the mortality of ascaris eggs is greater than or equal to 97%; the fertilizer has no pungent odor. The invention further discloses a preparation method of the biological bacterial fertilizer prepared from the poultry and livestock waste. The method comprises the step of preparing carrier materials; the prepared biological bacterial fertilizer is long in service life and especially beneficial to crop growth and seeding, and has beneficial strains, various enzymes and trace elements such as various vitamins, carbohydrate, cellulose and nucleic acid; the microbial activity of soil can be enhanced, the number of beneficial bacteria in root-layer soil is increased, and the activity of the beneficial bacteria in the root-layer soil is improved.

The invention relates to an application of a reporter plasmid for biological detection of radiation dose. The application comprises the following steps that a shuttle vector between radiation resistant bacteria and coliform bacteria is taken as a foundation so as to form a reporter plasmid of the shuttle vector which has luciferase gene activity and is positioned between the radiation resistant bacteria and the coliform bacteria; the reporter plasmid is transformed into a wild type R1 of radiation resistant bacteria to obtain transformant radiation resistant bacteria which are irradiated by gamma ray; and according to the luminous quantity of a character transformant, luminous quantities after irradiation by gamma rays in different doses are measured. The reporter plasmid can be used for biological detection of high radiation dose and low radiation dose, and can be widely used in the detection of nuclear facilities such as a nuclear power station, radiation irradiation facilities in a hospital, and the like, and other places with radioactive sources; moreover, the reporter plasmid can also be used in contamination detection for radioactive substances in an environment, and can induce a plurality of DNA damaging agents as a bioinductor; meanwhile, the reporter plasmid has a luciferase gene, and plays a positive role in realtime monitoring of aspects of procreation, distribution and environmental influence of a recombined transformant, and so on.

A preparing method of recombination heat resistance xylose/glucose isomerase, including: first, extracting genome DNA of thermozymes, amplificating PCR to obtain isomerase gene, inserting the gene in gene to express carrier pHsh, inverting coliform bacteria to obtain isomerase expression plasmid; second, mutagisming gene, changing usage frequency of codon and two-level structure of mRNA to obtain optimized isomerase expression plasmid; third, shifting the isomerase expression plasmid or optimized isomerase expression plasmid into coliform bacteria to obtain gene strains, and expressing xylose/glucose isomerase by thermally stimulating and inducing; finally, collecting cells, rupturing and centrifugating to obtain rough enzyme liquid, further purifying to obtain 90% purness of recombination heat resistance xylose/glucose isomerase. The invention first uses expression carrier pHsh to efficent express heat resistance xylose/glucose isomerase gene of thermozymes, producibility of recombination enzyme using this technque is more than 200 mg/L, the product has good stability and the method is easy to industrialized produce.

An ozonating water treatment and filtration apparatus is provided wherein a groundwater containing contaminates including hydrogen sulfide, ferrous oxide, carbon dioxide, manganese, arsenic, coliform bacteria and other pathogens and compounds, are removed by filtration utilizing a zeolite and pea gravel filter media and by application of an oxidant using low concentrations of ozone. A silent ozone generator and injector feature provides silent operation and no ozone pumping mechanism. The apparatus is self regenerating, low cost, and low maintenance requiring few consumables and minimal user intervention.