Escherichia coli coliform group detection kit

Abstract

The Escherichia coli coliform group detection kit comprises an upper box body and an Escherichia coli coliform group test piece, an upper cover is hinged to an opening in the top of the upper box body, and a plurality of detection grooves penetrating through the bottom wall are formed in the bottom wall of the upper box body. The interior of the detection groove is detachably connected with a test piece box for placing an escherichia coli coliform group test piece through an elastic fixing device; the bottom of the upper box body is movably connected with a culture box; according to the utility model, the culture box and the elastic fixing device are arranged, so that sample treatment and inoculation can be carried out in the upper box body firstly, and then an inoculated escherichia coli coliform test piece is transferred into the water bath of the culture box for culture; the electric heating tube and the thermometer are arranged in the culture box, the temperature of the water bath tank can be adjusted to 37 DEG C through water bath heating, the temperature is suitable for culturing coliform bacteria, and the detection result is more accurate.

Description

Technical Field

[0001] The utility model relates to the field of coliform bacteria detection, in particular to an Escherichia coli coliform bacteria detection kit. Background Art

[0002] Coliform bacteria is not a bacteriological classification name, but a term used in the field of sanitary bacteria. It does not represent a certain bacteria or a certain genus, but refers to a group of bacteria with certain characteristics related to fecal pollution. It is defined as: a gram-negative, non-embryo-forming bacillus that is aerobic and facultatively anaerobic and can decompose lactose to produce acid and gas at 37°C. It is generally believed that the bacteria in this group may include Escherichia coli, Citrobacter rodentium, Klebsiella aerogenes and Enterobacter cloacae.

[0003] In the related technology, a utility model patent with patent publication number CN207727081U was searched and proposed an Escherichia coli coliform detection kit, including a bottom box, an Escherichia coli coliform test piece and a box cover. The bottom box and the box cover are fixed together to form an openable and closable box structure. A groove is provided on the upper part of the bottom box, and an Escherichia coli coliform test piece is arranged inside the groove. The box cover is composed of a box wall and a polytetrafluoroethylene film layer. The middle part of the box wall is a hollow structure, and a polytetrafluoroethylene film layer is fixed to the middle part of the box wall.

[0004] According to the above-mentioned prior art, the inventors combined relevant technologies and found in practical applications that in the actual detection of Escherichia coli and coliform bacteria, the samples need to be inoculated and cultured. After the samples are inoculated, the added test pieces need to be placed in an incubator at 37 degrees and cultured for a period of time before observation. The detection kit will have inaccurate test results due to the lack of a culture process. Utility Model Content

[0005] In order to solve the problem of inaccurate detection results due to the lack of a cultivation process in the prior art, the utility model provides an Escherichia coli coliform detection kit;

[0006] The utility model provides a kit for detecting Escherichia coli and coliform bacteria using the following technical solutions:

[0007] An E. coli and coliform group detection kit comprises an upper box body and an E. coli and coliform group test piece, wherein an upper cover is hingedly connected to the top opening of the upper box body, a plurality of detection slots penetrating the bottom wall are opened on the bottom wall of the upper box body, and a test piece box for placing the E. coli and coliform group test piece is detachably connected to the inside of the detection slot through an elastic fixing device; a culture box is movably connected to the bottom of the upper box body;

[0008] Furthermore, the culture box is a hollow structure, a water inlet is provided on the top of the culture box, an electric heating tube is provided inside the culture box, and the top of the culture box is concave inwards and forms a water bath;

[0009] Furthermore, the inner side wall of the detection tank extends downward to form a channel, and the end of the channel can be pressed around the water bath tank;

[0010] Further, the elastic fixing device includes a limit pin and a spring; a receiving groove communicating with the detection groove is formed on the bottom wall of the upper box body, a limit pin is arranged in the receiving groove, one end of the spring is connected to the side wall of the limit pin, and the other end of the spring is connected to the inner side wall of the receiving groove; a through groove is formed on the top of the receiving groove, and the top of the limit pin passes through the through groove and extends outside the through groove; a fixing hole matching with the limit pin is formed on the side wall of the test piece box;

[0011] Furthermore, the top of the culture box is inwardly concave and forms a battery box, a lithium battery is arranged in the battery box, and the lithium battery is electrically connected to the electric heating tube;

[0012] Furthermore, a reference table is attached to the surface of the culture box, a thermometer is arranged on the side wall of the culture box, and a probe of the thermometer passes through the side wall of the culture box and enters the interior of the culture box;

[0013] Furthermore, a first connection portion is integrally formed on the bottom of the upper box body, and a second connection portion is integrally formed on the top of the culture box, and the first connection portion and the second connection portion form an interference fit.

[0014] In summary, the beneficial effects of the utility model are:

[0015] The utility model provides a culture box, and after the sample is inoculated into the Escherichia coli coliform test piece, the sample can be transferred into the culture box for culture; by providing an electric heating tube and a thermometer in the culture box, the water bath can be adjusted to 37 degrees through water bath heating, and this temperature is suitable for culturing the coliform group, so that the detection result is more accurate; by providing an elastic fixing device, the sample can be processed and inoculated in the upper box body first, and then the inoculated Escherichia coli coliform test piece can be transferred into the water bath of the culture box for culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the main view of the utility model;

[0017] Figure 2 This is a schematic diagram of the specific structure of the upper box body of the utility model;

[0018] Figure 3 It is a cross-sectional schematic diagram of the elastic fixing device of the utility model;

[0019] Figure 4 This is a schematic diagram of the bottom of the upper box body of the utility model;

[0020] Figure 5 This is a schematic diagram of the specific structure of the culture box of the utility model;

[0021] Figure 6 It is a cross-sectional schematic diagram of the culture box of the utility model.

[0022] As shown in the figure: 1-upper box body, 11-first connecting part, 12-second connecting part, 2-Escherichia coli coliform test piece, 3-upper cover, 4-detection slot, 5-test piece box, 51-fixing hole, 6-culture box, 61-water inlet, 62-electric heating tube, 63-water bath, 64-battery box, 65-lithium battery, 66-comparison table, 67-thermometer, 68-probe, 7-channel, 8-elastic fixing device, 81-limiting pin, 82-spring, 83-accommodating slot, 84-through slot. DETAILED DESCRIPTION

[0023] The following is combined with Figure 1 -Attached Figure 6 The utility model is further described in detail:

[0024] The utility model embodiment discloses a kit for detecting Escherichia coli and coliform bacteria, such as Figure 1 , Figure 2 As shown, an E. coli coliform test kit of the utility model comprises an upper box body 1 and an E. coli coliform test piece 2, an upper cover 3 is hinged at the top opening of the upper box body 1, a plurality of test slots 4 penetrating the bottom wall are provided on the bottom wall of the upper box body 1, and a test piece box 5 for placing the E. coli coliform test piece 2 is detachably connected to the inside of the test slot 4 through an elastic fixing device 8; a culture box 6 is movably connected to the bottom of the upper box body 1; in this embodiment, by providing the culture box 6, after the sample is inoculated into the E. coli coliform test piece 2, it can be transferred to the culture box 6 for culture. By providing the test piece box 5, the E. coli coliform test piece 2 can be pasted into the test piece box 5, and the inside of the test piece box 5 can be disinfected after the detection is completed. At the same time, since the E. coli coliform test piece 2 is relatively thin, the test piece box 5 can better transfer the inoculated E. coli coliform test piece 2 into the culture box 6. By setting up the elastic fixing device 8, sample processing and inoculation can be carried out in the test piece box 5, and then the elastic fixing device 8 is operated to transfer the test piece box 5 to the water bath 63 of the culture box 6 for culture, so that the inoculated Escherichia coli coliform test piece 2 is placed in the culture box 6 at an ambient temperature of 37 degrees. After culturing for 15-24 hours, the result of the test piece is interpreted, and the detection of the sample is more accurate at this time.

[0025] like Figure 5 , Figure 6 As shown, the culture box 6 is a hollow structure, a water injection port 61 is provided on the top of the culture box 6, an electric heating tube 62 is provided inside the culture box 6, and the top of the culture box 6 is recessed inwards and forms a water bath 63; in this embodiment, water can be added to the box or drained out through the water injection port 61 on the top of the culture box 6, and the water injection port 61 can be closed with a rubber plug after the operation is completed. By providing the electric heating tube 62 in the culture box 6, the water temperature inside the culture box 6 can be maintained at 37 degrees, which is suitable for culturing coliform bacteria and making the detection results more accurate.

[0026] like Figure 4 As shown, the inner wall of the detection groove 4 extends downward to form a channel 7, and the end of the channel 7 can be pressed on the four sides of the water bath 63; in this embodiment, by setting the channel 7, the test piece box 5 can slide along the inside of the channel 7 and accurately fall into the water bath 63, thereby ensuring the stability of the detection.

[0027] like Figure 2 , Figure 3 As shown, the elastic fixing device 8 includes a limit pin 81 and a spring 82; the bottom wall of the upper box body 1 is provided with a receiving groove 83 which is communicated with the detection groove 4, and a limit pin 81 is arranged in the receiving groove 83, and one end of the spring 82 is connected to the side wall of the limit pin 81, and the other end of the spring 82 is connected to the inner side wall of the receiving groove 83; a through groove 84 is provided at the top of the receiving groove 83, and the top of the limit pin 81 passes through the through groove 84 and extends outside the through groove 84; a through groove 84 is provided on the side wall of the test piece box 5 to cooperate with the limit pin 81 In this embodiment, by providing a stop pin 81 and a spring 82, the test piece box 5 can be fixed by the stop pin 81 in the detection slot 4. When in use, the stop pin 81 outside the sliding slot 84 is returned to the receiving slot 83 and the spring 82 is compressed, and the test piece box 5 is inserted into the detection slot 4. When the test piece box 5 reaches the predetermined position, the stop pin 81 will extend from the receiving slot 83 under the push of the spring 82 and be inserted into the fixing hole 51 on the side wall of the test piece box 5. Preferably, the end of the stop pin 81 is formed with a driving inclined surface.

[0028] like Figure 5 , Figure 6As shown, the top of the culture box 6 is concave inward and forms a battery box 64, in which a lithium battery 65 is arranged, and the lithium battery 65 is electrically connected to the electric heating tube 62; a reference table 66 is pasted on the surface of the culture box 6, and a thermometer 67 is arranged on the side wall of the culture box 6, and a probe 68 of the thermometer 67 passes through the side wall of the culture box 6 and enters the interior of the culture box 6; in this embodiment, by providing the battery box 64 and the lithium battery 65, the carrying function is added to the E. coli coliform detection kit, and detection can be performed in more usage scenarios. By providing the thermometer 67 and the probe 68, the cultivation temperature in the culture box 6 can be intuitively displayed.

[0029] like Figure 4 , Figure 5 As shown, a first connecting portion 11 is integrally formed at the bottom of the upper box body 1, and a second connecting portion 12 is integrally formed at the top of the culture box 6, and the first connecting portion 11 and the second connecting portion 12 form an interference fit; in this embodiment, the upper box body 1 and the culture box 6 form an interference fit through the first connecting portion 11 and the second connecting portion 12 to achieve a pluggable movable connection.

[0030] The implementation principle of the embodiment of the utility model is:

[0031] The limiting pin 81 outside the sliding through slot 84 is returned to the containing slot 83 and the spring 82 is compressed, and the test piece box 5 is inserted into the detection slot 4. When the test piece box 5 reaches the predetermined position, the limiting pin 81 is pushed out from the containing slot 83 by the spring 82 and inserted into the fixing hole 51 on the side wall of the test piece box 5; the E. coli coliform test piece 2 is pasted into the test piece box 5, and the sample to be tested is dripped onto the surface of the E. coli coliform test piece 2 in the test piece box 5 through a dropper and left to stand for a while; The water inlet 61 at the top of the culture box 6 is used to inject water into the box and then the water inlet 61 is closed by a rubber stopper; the electric heating tube 62 is started to heat the water inside the culture box 6, and the reading on the thermometer 67 is read to maintain the water temperature inside the culture box 6 at 37 degrees; the limit pin 81 outside the sliding groove 84 is returned to the receiving groove 83, and the test piece box 5 will slide along the inside of the channel 7 and fall into the water bath 63. After 15-24 hours of incubation, the upper box body 1 is opened and the results of the test piece are interpreted by the reference table 66.

[0032] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. The various components mentioned in the utility model are common technologies in the existing field. The technicians in this industry should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only to illustrate the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model will have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. An Escherichia coli coliform detection kit, comprising an upper box body (1) and an Escherichia coli coliform test sheet (2), wherein an upper cover (3) is hingedly connected to the top opening of the upper box body (1), characterized in that: The bottom wall of the upper box body (1) is provided with a plurality of detection grooves (4) penetrating the bottom wall, and the inside of the detection groove (4) is detachably connected to a test piece box (5) for placing an Escherichia coli coliform test piece (2) via an elastic fixing device (8); the bottom of the upper box body (1) is movably connected to a culture box (6); the culture box (6) is a hollow structure, the top of the culture box (6) is provided with a water injection port (61), the inside of the culture box (6) is provided with an electric heating tube (62), the top of the culture box (6) is recessed inwardly and forms a water bath (63); the inner side wall of the detection groove (4) extends downward to form a channel (7), the end of the channel (7) can be pressed on the water bath The elastic fixing device (8) comprises a limit pin (81) and a spring (82); the bottom wall of the upper box body (1) is provided with a receiving groove (83) which is in communication with the detection groove (4); a limit pin (81) is arranged in the receiving groove (83); one end of the spring (82) is connected to the side wall of the limit pin (81); the other end of the spring (82) is connected to the inner side wall of the receiving groove (83); a through groove (84) is provided at the top of the receiving groove (83); the top of the limit pin (81) passes through the through groove (84) and extends outside the through groove (84); and a fixing hole (51) which matches with the limit pin (81) is provided on the side wall of the test piece box (5).

2. The Escherichia coli coliform detection kit according to claim 1, characterized in that: The top of the culture box (6) is recessed inwards and forms a battery box (64), in which a lithium battery (65) is arranged, and the lithium battery (65) is electrically connected to the electric heating tube (62).

3. The Escherichia coli coliform detection kit according to claim 2, characterized in that: A reference table (66) is pasted on the surface of the culture box (6), a thermometer (67) is arranged on the side wall of the culture box (6), and a probe (68) of the thermometer (67) passes through the side wall of the culture box (6) and enters the interior of the culture box (6).

4. The Escherichia coli coliform detection kit according to claim 1, characterized in that: The bottom of the upper box body (1) is integrally formed with a first connection portion (11), and the top of the culture box (6) is integrally formed with a second connection portion (12), and the first connection portion (11) and the second connection portion (12) form an interference fit.

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