Application of lactobacillus delbrueckii
A technology of Lactobacillus delbrueckii and bacterial liquid, applied in the application field of Lactobacillus delbrueckii, can solve the problems of high cost, harm to human health, poor preservation methods of fruits and vegetables, etc., and achieves the effect of improving the preservation effect and inhibiting harmful bacteria.
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[0030] Example 1
[0031] A method for preserving strawberries, which includes the following steps:
[0032] (a) Activate Lactobacillus delbrueckii twice to obtain Lactobacillus delbrueckii bacterial liquid, wherein the medium used for activation is MRS liquid medium, the activation temperature is 36°C, and the activation time is 26h each time;
[0033] (b) Use MRS liquid medium to dilute Lactobacillus delbrueckii to a concentration of 3×10 8 cfu / mL, centrifuge for 8min at 6℃ and 9000r / min, collect the supernatant;
[0034] (c) Soak the fruits and vegetables in the supernatant for 3s, dry them at room temperature, and store the strawberries.
Example Embodiment
[0035] Example 2
[0036] A method for preserving strawberries, which includes the following steps:
[0037] (a) Activate Lactobacillus delbrueckii 4 times to obtain Lactobacillus delbrueckii bacterial liquid, wherein the medium used for activation is MRS liquid medium, the activation temperature is 38°C, and each activation time is 22h;
[0038] (b) Use MRS liquid medium to dilute Lactobacillus delbrueckii to a concentration of 5×10 8 cfu / mL, centrifuge for 12min at 3℃, 7000r / min, and collect the supernatant;
[0039] (c) Soak the fruits and vegetables in the supernatant for 2 seconds, dry them at room temperature, and then store the strawberries.
Example Embodiment
[0040] Example 3
[0041] A method for preserving strawberries, which includes the following steps:
[0042] (a) Activate Lactobacillus delbrueckii three times to obtain the Lactobacillus delbrueckii bacterial solution, wherein the medium used for activation is MRS liquid medium, the activation temperature is 37°C, and the activation time each time is 24h;
[0043] (b) Use MRS liquid medium to dilute Lactobacillus delbrueckii to a concentration of 1×10 8 cfu / mL, centrifuge for 10min at 4℃ and 8000r / min, collect the supernatant;
[0044] (c) Soak the fruits and vegetables in the supernatant for 2 seconds, dry them at room temperature, and then store the strawberries.
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