108 results about "Bacteria coliforms" patented technology

The invention provides a method for suppressing the gene expression of insects through feeding dsRNA, which belongs to the growth development field of adjusting and controlling insects through naturally feeding the dsRNA of the silenced and specific gene. The method selects a gene fragment SeA3 on chitin synthetase enzyme gene SeCHS-A, and studies the impact on the insects after the natural feeding. The method mainly comprises the steps: a proper expression vector L4440 is selected to construct a recombinant expression vector L4440-SeA3 with a purpose gene fragment SeA3; the recombinant vector is induced and expressed in specific recipient cell coliform bacteria HT115 (DE3) under vitro adaptive condition; and the recipient bacterium htLSA3 which expresses dsRNA is added into the feedstuff of the insects, the insect larvae are naturally fed with the feedstuff, the situation of the insects suppressing the gene expression is detected after the feeding, and the change of the phenotype of the insects is recorded. By adopting natural feeding to suppress the expression of the insect genes, the method explores new paths for controlling the insects.

Qualitative and quantitative electrochemiluminescent assays for analytes of interest present in multicomponent liquids are provided. These methods comprise contacting a sample with a reagent labeled with an electrochemiluminescent chemical moiety and capable of combining with the analyte of interest, exposing the resulting sample to electrochemical energy and detecting electromagnetic radiation emitted by the electrochemiluminescent chemical moiety. Further provided are methods for detecting and identifying the presence of a multiplicity of analytes in a liquid food or food homogenate. These methods comprise immersing a diagnostic reagent holder, provided with a multiplicity of reagents, into the food or food homogenate, removing the diagnostic reagent holder from the liquid food or food homogenate, and detecting and identifying the presence of a multiplicity of analytes of interest bound to the diagnostic reagent holder, thereby detecting and identifying the presence of a multiplicity of analytes of interest in the food or food homogenate. The invention further provides an enzyme immunoassay for coliform bacteria. This assay comprises inoculating a sample into a suitable medium for coliform reproduction, immobilizing coliforms present in the medium to a suitable surface, treating the surface with an antibody directed to the immobilized coliforms and detecting the presence of the immobilized coliforms immobilized to a suitable surface.

The invention discloses a testing piece for quickly testing microorganisms, as well as a preparation method and application thereof. The testing piece comprises a soleplate, a film with a hollow part in the middle and a plastic film. The bottom surface of the film with the hollow part in the middle is adhered to the soleplate; a groove culture area is formed at the hollow part in the middle of the film; a color development culture medium is arranged in the groove culture area; a cold water condensable gellant is adhered to the bottom surface of the plastic film; and the bottom surface of the plastic film and the upper surface of the film are adhered to the edge at one side. After the upper and the lower layers of the testing piece are adhered, irradiation and sterilization packages are used for standby. The testing piece can be applied to the detection of total colony count, coliform bacteria, coliform group, salmonella, staphylococcus aureus, subsidiary haemolytic vibrio, pseudomonas aeruginosa and other food-borne causative organisms and has the advantages of simple operation, convenient carrying, short testing time, accurate result and double-sided bacteria count.

The invention is relative to a novel method for the detection of coliforms, which is based on the use of a mixture of amino acids to promote the expression of inducible enzymes, in absence of cell growth. This enzyme-inducing solution can be used for the rapid detection of the enzymes β-glucuronidase and/or β-galactosidase in a very small number of E. coli and/or total coliforms.

The invention relates to a method for detecting bacteria in drinking water and surface water, especially a method for simultaneous specific detection of bacteria from the Legionella species and the Legionella pneumophila species by in situ hybridization. The invention also relates to a method for specific detection of faecal streptococci by in situ-hybridization and a method for simultaneous specific detection of coliform bacteria and bacteria of the Escherichia coli species, in addition to corresponding oligonucleotide probes and kits enabling said inventive method to be carried out.

The invention discloses tetanus toxin T cell epi position peptide and human B lymphocyte activate and gene interfusing albumen. It could activate CD4⁺T cell to take cell action, promote body liquid immunity level. It adopts step clone to construct TT and BLyS interfusing gene and gain high efficiency expression in coliform bacteria. It conquers the immunity tolerate of the body.

The invention discloses a nanometer precipitated phase martensitic antibiotic stainless steel. Granular nanometer scale precipitated phases Epsilon-Cu are dispersed and distributed evenly in a stainless steel matrix, to cause the stainless steel to have bacteria resistant performance. The weight percentage of the chemical compositions of the stainless steel is as follows: C is 0.10 to 0.40 percent, Cr is 12.00 to14.00 percent, Cu is 1.50 to 3.50 percent, RE is 0.04 to 0.10 percent, Mg is 0.05 to 0.15 percent, Ti is 0.03 to 0.10 percent, Si is lower than or equal to 0.60 percent, Mn is lower than or equal to 0.60 percent, P is lower than or equal to 0.03 percent, S is lower than or equal to 0.03 percent, and the residual is Fe. The components of the invention are simple, alloying elements are few, and bacterium such as coliform bacteria, salmonella typhiurium, staphyloccocus aureus rosenbach and white candidiasis contacted with the stainless steel can be effectively killed. The bacteria resistant performance is good and durative, and the invention can be widely applied to the fields of food processing, kitchen dining, daily electrical appliance, medical appliance, and equipment, etc.

The invention provides a coliform bacteria rapid detection cold water gel scrip, and the detection cold water gel scrip is a hygiene inspection product special for rapid detection of coliform bacteria number in food and tableware. Through reasonable ratio of culture medium components and cold water gel components, scientific production process and the appropriate packaging, the product cost is low, the operation method is simple and easy to operate, detection procedure is simple and fast, so that cumbersome tube fermentation culture and physical and chemical test are simplified and can be completed in one step, sensitivity is good, specificity is strong, results are accurate, the national standard method coincidence rate is high; meanwhile antibacterial substances are added into the culture medium to reduce the interference of other microorganisms. The rapid detection scrip can achieve quick and accurate detection and qualitative and quantitative analysis of coliform bacteria, and can be applied to the rapid detection of the coliform bacteria in food and tableware.

The invention discloses protein ompK subunit vaccine of assistant haemolysis vibrio extine and a preparation method thereof. The vaccine is PBS solution which converts the recombination protein of the coliform bacteria of recombination prokaryon expression plasmid pET30a-ompK expressed by inducing and after being purified; the concentration of the PBS solution is 0.25-0.5mg/ml. The method has the steps that: firstly, the extraction of an assistant haemolysis vibrio full gene group, the overall length of extine protein ompK DNA and the clone of a mature peptide coded sequence; secondly, the construction of a prokaryon expression plasmid of the ompK mature peptide coded sequence, thirdly, the obtaining way of the recombination ompK protein; fourthly, the detection to the immunity way and the immunity effect of a large yellow croaker with recombination ompK protein. The invention provides the preparation method of the assistant haemolysis vibrio ompK protein subunit vaccine, and simultaneously provides the detection method of the immunity effect of the large yellow croaker with recombination ompK protein, the preparation method is simple, and the usage is convenient.

This invention relates to a culture medium for the rapid detection of coliform and coliform groups, which is mixed from distilled water solution containing peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free), the solution of indophenol-beta-D-glucoside and solution of cefsulodin. Peptone, yeast extract, lactose, 4-methylumbelliferone-beta-D-galactoside, sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium lauryl sulfate and agar (or agar-free) are weighed according to a certain criterion and added to distilled water for heat dissolution. The consequent solution is adjusted to a certain pH value, put into a high pressure sterilizer for sterilization and then added with filtrated and sterilized indophenol-beta-D-glucoside solution and cefsulodin solution after the temperature lowered to 50 deg.Cí‚5 deg.C. This is useful in either qualitative or quantitative detection of coliform and coliform groups in drinking water, environmental water and foods, high efficiency and accurate detection result.

The invention discloses a manufacture method of fresh-keeping garlic rice, which has the main technical scheme that garlic is manually divided into cloves and is peeled, the peel of the garlic is removed, then, the garlic is soaked for 10 minutes in color protecting and sterilization liquid, and the garlic carries out vacuum packing and refrigeration. The invention has the advantages of simple production process and fresh garlic rice. The total number of colonies of 1g sample bacteria is smaller than 10000, and the coliform bacteria of 100g sample feces is less than 3. The bacillus cereuses are less than 3/g, and the shelf life is 90 days.

Disclosed are a DNA chip and a kit capable of quickly and accurately detecting or genotyping the highly prevalent and important eleven microbes causing sexually transmitted diseases (STD) Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, syphilis-causing treponema, pallidum, chancroid-causing Haemophilus ducreyi, genital herpes-causing herpes simplex virus 1 and 2, human papillomavirus (HPV) and Trichomonas vaginalis and three related organisms Candida albicans, Gardnerella vaginalis and coliform bacteria and analyzing antibiotic resistance against tetracycline and lactam antibiotics, and a method for detecting or genotyping using the same. According to the present invention, the presence, genotype and antibiotic resistance of the fourteen organisms can be analyzed quickly and accurately from a DNA sample. With excellent sensitivity, specificity, reproducibility and accuracy of the 14 STD-causing and related microorganisms may be automatically identified quickly and accurately from multiple samples, and selection of antibiotics may be aided.

An object of the present invention is to provide a method for producing a chlorella fermented food in which a flavor and odor that are peculiar to algae are reduced while production of pheophorbide is suppressed. The method for producing a chlorella fermented food according to the present invention can be achieved by using a chlorella negative in coliform bacteria count, and having a content of free pheophorbide equal to or less than 18 mg % and a viable general bacteria count equal to or less than 8000 cfu/mL, or using a chlorella subjected to a heat sterilization treatment to ferment the chlorella with a baker's yeast. The baker's yeast is blended with the chlorella at a ratio of preferably 0.1 to 15% by weight. Further, the fermentation treatment is preferably carried out in the presence of glucose and a lactic acid bacterium.

The present invention relates to a method of detecting coliform bacteria in water from reflected light, and also includes devices for the measurement, calculation and transmission of data relating to that method.

The invention relates to a method of make single valve RT-PCR dectecting masculine comparing virulence haemorrhagic septicemia virus, fish infectivity hemocytopoietic organ putrescence and infectivity pancreas putrescence germina. Its feature is selecting G gene from virulence haemorrhagic septicemia virus, VP gene from fish infectivity hemocytopoietic organ putrescence and N gene from infectivity pancreas putrescence germina, inserting into pUC57 particle carrier and transfect to DH5 alpha coliform bacteria to take in vitro expression, and extending the section 625bp, 371bp, 206bp. It has very high use value.

The invention provides a recombinant porcine interferon IFN beta1-Fc fusion protein as well as an encoding gene and expression, purification and inclusion body renaturation methods thereof, belonging to the biological genetic engineering field. The IFNbeta can enhance the immunity of pig and has a good application prospect in the veterinary medicine industry. However, natural porcine IFNbeta1 is less in expression quantity and is insufficient for research and development and application and has the deficiency of quick plasma clearance rate. The invention provides the recombinant porcine interferon IFN beta1-Fc fusion protein applicable to a coliform bacteria prokaryotic expression system. Part of the porcine IFNbeta1 is all sequences in an ectoenzyme area of the porcine IFNbeta1. The Fc section comprises a hinge region, a CH2 region and a CH3 region of an antibody. The porcine IFNbeta1 and the Fc section are directly fused. The fusion protein provided by the invention not only maintains the biological activity of the original protein IFNbeta1 to a great extent, but also extremely prolongs the half-life period of the original protein IFNbeta1, thereby providing the opportunity of industrialized development of the fusion protein.

The invention discloses a coli group and colibacillus testing medium which is prepared by dissolving 20 grams of peptone, 5 grams of lactose, 2 grams of bile salt, 1.5 grams of monopotassium phosphate, 4 grams of monopotassium phosphate, 5 grams of sodium chloride, 0.1 gram of 5-bromo-4-chloro-3-indole galactoside, 0.1gram of 4- methylumbelliferone glucuronide, 1 gram of aspartic acid, 0.5 gram of casamino acid, 1 gram of yeast extracts and 20 grams of agar in 1000ml of deionized water, and the coli group and colibacillus testing medium is not only used as a plate medium, can also be used as an analoids form, and is low in cost and convenient to popularize and use.

The invention belongs to the technical field of food microorganisms, and relates to a beverage fermented through hericium erinaceus and lactic acid bacteria, and a preparation method of the beverage.The obtained beverage fermented through hericium erinaceus and lactic acid bacteria has an orange yellow color and uniform luster, is a cloudy beverage, has no precipitation and layering, is sweet andsour in taste and strong in flavor, has unique flavors of fermented hericium erinaceus and lactic acid bacteria fermentation, and has no bad peculiar smell. The content of soluble solids is 9.8%, thecontent of hericium erinaceus polysaccharides is 420 mg/mL, the content of total acid is 0.65%, the pH value is 4.5, the sum of bacteria is 100 fu/mL or below, and the coliform bacillus flora is 3MPN/100mL or below. No pathogenic bacterium is detected, so that the beverage meets national standards.

The invention provides a water purification process for preparing living water. The water purification process comprises the following steps carried out on the water to be treated: filtering to remove suspended solids; aeration treatment to oxidize Fe<2+> and Mn<2+> in water; active biological membrane contact oxidation and filtration treatment to remove iron and manganese; activated carbon adsorption treatment to remove organic pollutants and pesticides; nanofiltration treatment to remove heavy metal ions; and sterilization. The invention has the beneficial effect that the process can purify water source with complex components (e.g. riverbed groundwater containing coliform bacteria, turbidity, heavy metal ions and organic pollutants) into living water meeting the national standard.

The invention belongs to the field of quality control in an aspect of microbial detection, and particularly relates to a coliform standard sample in food and a preparation method thereof. The coliform standard sample in food comprises a target flora and a background flora, wherein the target flora consists of colon bacillus, klebsiella pneumoniae, enterobacter cloacae and citrobacter freundii; the background flora consists of rhodococcus equi, bacillus cereus and staphylococcus aureus. The uniformity and the stability conform to the capability verification requirements; the stability of a test sample can be ensured to the maximum degree; even when the content of colon bacillus changes in the transportation and storage process, the total quantity of the coliform cannot be influenced in a sense of statistics. The sample preparation method has the advantages that the process is simple; the success ratio is high.

The invention relates to a preparation method of a standard sample for establishing a quantitative curve of coliform bacteria, an establishment method and an application of the quantitative curve. Firstly a liquid sample to be detected is a sample without coliform bacteria of a same product, and the liquid sample is subject to pH value adjustment to prepare a sterile sample; a standard bacterial strain of coliform bacteria is added into the sterile sample; the pH value is adjusted to prepare a sample rich in bacteria; the sample rich in bacteria is diluted by the sterile sample for ten times in a progressive decrease tendency to prepare a standard sample. The standard sample is cultured, and detection time for the pH value reaching a certain threshold is detected. And the number of coliform bacteria in the standard sample is detected by a plate method. A quantitative curve of coliform bacteria is plotted based on the detection time and the detection result of the plate method. The liquid sample is taken and subject to pH value adjustment so as to prepare liquid to be detected; detection time is obtained by the above method; and the number of coliform bacteria in the liquid sample is obtained by comparing with the quantitative curve. The method balances the background values between samples, so the detection accuracy is improved.