9 results about "Bacteria coliforms" patented technology

The embodiment of the invention discloses application of lactobacillus delbrueckii. The application is characterized in that the lactobacillus delbrueckii is applied to inhibition of mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria. The lactobacillus delbrueckii has a preservation number of CGMCC No.11247. In the lactobacillus delbrueckii activating process, bacteriocin can be generated and can remarkably inhibit the mesophilic aerobic bacteria, saccharomycetes, mould and coliform bacteria on the surfaces of fruits and vegetables, so that the preservation effect of thefruits and vegetables can be improved. Furthermore, secondary metabolites of the lactobacillus delbrueckii can be biodegraded and digested by human bodies, and are healthy and harmless to human bodies.

The invention discloses a low-temperature hot air drying preparation process of low-sugar preserved mangoes. The preparation process comprises the following steps: S1, pretreating mangoes; s2, blanching is conducted; s3, hardening and color protection; s4, rinsing is carried out; s5, performing sugaring; s6, sugar draining; s7, drying; and S8, cooling. The sensory score of the product prepared by the process is 86.96, and the product is golden in color, rich in fragrance and palatable in sour and sweet taste. The total sugar content is 35.88% and is obviously lower than that of the traditional high-sugar preserved fruit. And the water content is 21.78%. The hardness is 3216.35 + / -g, the cohesiveness is 0.88, the chewiness is 3155.35, the resilience is 0.56, and the texture property is good. The total bacterial colony number 1t; 10 CFU / g, and coliform bacteria are not detected.

Existing predictive models employ fixed algorithms and parameters, which may not sufficiently account for dynamic nature of environmental conditions and microbial populations. The present disclosure receives data from one or more sensors specific to a water sample, information specific to Geographic Information System (GIS) and data specific to one or more process parameters. One or more sensors data is mapped with information specific to the GIS. Mapped data along with data specific to one or more process parameters are preprocessed using one or more data preprocessing techniques. The preprocessed data is fed into one or more trained Machine Learning (ML) models to predict likelihood of Escherichia coli. A correlation matrix of one or more parameters comprised in the preprocessed data and the predicted likelihood of E. coli is obtained. Contamination risks associated with the water sample are assessed based on the correlation matrix obtained.

A diagnostic kit and method for detecting urinary tract infections (UTIs) and bacteremia using a drinking water test in low-resource settings is provided. The kit includes a first bag containing a first culture medium capable of detecting Gram-negative and coliform bacteria through colorimetric and fluorescence indicators, and a second bag containing a second culture medium capable of detecting Gram-positive bacteria through colorimetric indicators. A method of using the kit involves inoculating a sample into each bag, incubating the bags, and observing for color changes and fluorescence to indicate bacterial presence. The kit enables rapid, cost-effective detection of bacterial pathogens in urine and blood samples without specialized laboratory equipment, facilitating early diagnosis and treatment in resource-limited environments.

The invention relates to the technical field of high polymer materials, and particularly discloses a degradable antibacterial modified ABS material and a preparation method thereof.The degradable antibacterial modified ABS material is prepared from, by mass, ABS resin, auxiliaries, an initiator, a heat stabilizer, antioxidant particles, a dispersion compatilizer and a composite antibacterial agent; the composite antibacterial agent is mainly used for releasing zinc ions for bacteriostasis, added hemicellulose is treated to react with zinc phosphate and phosphite to form a stable zinc ion release channel while being used for bacteriostasis, and antistatic particles are treated and mixed with ferric oxyhydroxide to modify diatomite to adsorb and destroy a bacterial structure so as to play a role in bacteriostasis and antioxidation. The prepared modified ABS material is a green degradable material with effective antibacterial performance, the antibacterial rate of the modified ABS material on escherichia coli, staphylococcus aureus and other florae can reach 99% or above, and meanwhile the modified ABS material has good mechanical performance and flame retardant performance and is biodegradable.

The invention belongs to the technical field of microbiological detection, and particularly relates to a method for rapidly detecting coliforms, which comprises the following steps: (1) mixing a sample to be detected with a diluent to obtain a sample solution; (2) centrifuging the sample liquid to remove supernate, and uniformly mixing precipitate with a culture medium to obtain suspension liquid; the culture medium is an EC-MUG culture medium or a mixture of an EC culture medium and FDG; (3) generating water-in-oil liquid drops from the suspension through a microfluidic liquid drop technology; (4) culturing the liquid drops in an incubator for a period of time; and (5) scanning the cultured liquid drops by using exciting light, detecting the total number of the liquid drops and the fluorescence intensity of each liquid drop, and then calculating the concentration of the coliform bacteria by using a Poisson distribution formula. According to the invention, the coliform group is divided into single bacterium liquid drops by adopting a microdroplet generation technology, and the total number of the coliform group is detected by utilizing an enzyme substrate luminescence principle and a fluorescence scanning counting technology, so that the rapid and accurate detection of the coliform group is realized.

The utility model relates to the technical field of bacterium detection equipment, and particularly discloses a coliform group single-tube quantitative detection device, which comprises a culture tube, a detection tube, a reducer tube, a piston and a silica gel foam plug, the surface of the detection tube is coated with a color-bacterium concentration relation table, a liquid discharge tube is arranged in the tube wall of the culture tube in a penetrating manner, and the liquid discharge tube is connected with the silica gel foam plug. A valve is connected to the portion, outside the culture tube, of the liquid discharging tube, the reducer tube comprises a small-diameter section, a first large-diameter section and a second large-diameter section, the first large-diameter section and the second large-diameter section are connected to the two ends of the small-diameter section respectively, the first large-diameter section is connected to the top of the culture tube in a sealed mode, and a silica gel foam plug is clamped in the second large-diameter section. The piston is connected into the small-diameter section in a sealed and sliding mode, the top of the piston is connected with a drawing rod, the drawing rod penetrates through the silica gel foam plug, and the culture tube achieves the effect of facilitating sampling under the condition that ventilation and bacterium isolation are guaranteed.

The invention discloses a novel gel type dairy product and a preparation process thereof. The novel gel type dairy product comprises a jasmine flower extracting solution, pure milk, white granulated sugar, gelatin and a 0.1% five-bacterium type lactic acid bacteria leavening agent. According to the preparation process of the novel gel type dairy product, the active ingredients of the jasmine flowers are extracted by adopting a cold extraction-distillation combined method, and the influence of multi-factor interaction on sensory scoring is solved through an orthogonal experimental design. Physicochemical and microbiological detection shows that the soluble solid content of the product is 18%, the acidity is 84 DEG T, the protein content is 4.51 g / 100g, the viable count of lactic acid bacteria is 4.7 * 10 < 8 > CFU / mL, the total number of bacterial colonies is only 1 CFU / g, coliform bacteria are not detected and are all superior to national standard requirements, and high nutritional value and safety are guaranteed. The product has fermented frankincense of yoghourt and fresh flower fragrance of jasmine flowers, is fine and smooth in taste and palatable in sourness and sweetness, and breaks through the limitation that traditional jelly is single in nutrition and insufficient in functionality.

The application discloses a microfluidic chip and a method for detecting water quality by embedding coliform group in alginate hydrogel, and the microfluidic chip comprises a sample inlet, an oil phase inlet, an alginate aqueous solution inlet, a droplet mixing flow channel, an incubation chamber and an outlet; wherein the oil phase inlet is connected with the sample flow channel through two oil phase flow channels which are divided into two parts and meet at a cross-shaped port, the alginate aqueous solution inlet is connected with the flow channel from the cross-shaped port through a water phase flow channel and meets at a T-shaped port; a contraction section flow channel and a first serpentine flow channel are sequentially arranged between the T-shaped port and the droplet mixing flow channel, and are respectively used for extruding and fusing the bacterial liquid droplet and the alginate droplet. The application solves the problem that the detection effect is poor due to the spontaneous fusion of droplets caused by the increase of incubation temperature in the prior art by combining the alginate with the calcium salt solution to form the hydrogel through ion cross-linking, and has a good application prospect in the detection of coliform group in water samples.