Real-time monitoring and early warning system for coliforms in direct drinking water

Abstract

The invention discloses a real-time monitoring and early warning system for coliforms in direct drinking water, and belongs to the technical field of direct drinking water coliform monitoring, the real-time monitoring and early warning system comprises a sampling module, the sampling module is in signal connection with a preprocessing module, the preprocessing module is in signal connection with a detection module, and the detection module is in signal connection with a control module. According to the system, continuous and online monitoring of coliform bacteria in direct drinking water can be achieved, water quality abnormity can be found in time, the fluorescent quantitative PCR technology is adopted, the detection speed is high, a detection result can be obtained in a short time, early warning can be conducted in time, meanwhile, the water quality is treated through the pretreatment module, and the detection sensitivity is improved; according to the water quality monitoring system based on the data management module, the requirement of the GB19298-2014 standard can be met, the whole process from sampling to early warning is automatic, manual intervention and operation errors are greatly reduced, the detection accuracy is improved, the system can record and analyze historical monitoring data through the data management module, a basis is provided for water quality management, and then detection personnel can conveniently manage the data.

Description

Technical Field

[0001] The present invention belongs to the technical field of monitoring the coliform group in direct drinking water, and particularly relates to a real-time monitoring and early warning system for the coliform group in direct drinking water. Background Technique

[0002] Drinking water is a basic requirement for human survival and health, and water accounts for 60%-70% of the human body weight and is the basis for maintaining life activities. The traditional detection method for coliform group in drinking water mainly relies on laboratory culture, which has the disadvantages of long detection cycle, cumbersome operation, and inability to monitor in real time. With the improvement of people's requirements for drinking water safety, it is of great significance to develop a monitoring system for the coliform group in direct drinking water that can monitor in real time and give early warnings quickly. Summary of the Invention

[0003] The purpose of the present invention is to provide a real-time monitoring and early warning system for the coliform group in direct drinking water, so as to solve the problems in the above background technique that the traditional detection method for coliform group in drinking water mainly relies on laboratory culture, which has the disadvantages of long detection cycle, cumbersome operation, and inability to monitor in real time.

[0004] To achieve the above purpose, the present invention provides the following technical solution: A real-time monitoring and early warning system for the coliform group in direct drinking water, including a sampling module, the sampling module is signal-connected to a pretreatment module, and the pretreatment module is signal-connected to a detection module. At the same time, the detection module is signal-connected to a control module, the control module is signal-connected to an early warning module, and the early warning module is signal-connected to a data management module;

[0005] As a further preference of this technical solution: S1: The system regularly collects water samples and performs pretreatment;

[0006] S2: Specific primers and probes are used to amplify and detect the DNA of the coliform group in the water sample, and the change of the fluorescence signal is monitored in real time;

[0007] S3: According to the change curve of the fluorescence signal, the threshold cycle number (Ct value) method is used to quantitatively analyze the concentration of the coliform group in the water sample;

[0008] S4: The detection result is compared with the limit value specified in the GB19298-2014 standard to determine whether the water quality is qualified;

[0009] S5: When the detection result exceeds the standard, the system triggers an early warning mechanism and issues an early warning signal;

[0010] S6: The system stores data such as the detection result and early warning information in the database;

[0011] As a further optimization of this technical solution: The sampling module includes a micro peristaltic pump and a flow meter, and electrically connects the micro peristaltic pump and the flow meter, which is used for automatic collection and quantitative control of water samples. At the same time, the micro peristaltic pump extracts water samples by means of hose extrusion, which can avoid pollution. Secondly, the flow meter can monitor and adjust the sampling volume in real time to ensure that the amount of water samples collected each time is consistent (such as a fixed 10 mL / time).

[0012] As a further optimization of this technical solution: The pretreatment module performs operations such as filtering and concentration on water samples, and the filtering is divided into primary filtering and fine filtering. At the same time, centrifugal concentration method and membrane filtration concentration method are adopted.

[0013] As a further optimization of this technical solution: The detection module uses a rapid detection method based on fluorescence quantitative PCR technology to detect coliform bacteria in water samples. The PCR detection process steps are in turn: DNA extraction, PCR reaction system construction, real-time fluorescence monitoring, and data analysis.

[0014] As a further optimization of this technical solution: The control module uses an embedded system or an industrial computer to control, collect, and process data of each module of the system.

[0015] As a further optimization of this technical solution: When the detection module detects that the coliform bacteria exceed the standard, it issues a warning signal through means such as sound and light alarm and text message notification.

[0016] As a further optimization of this technical solution: The data management module can record historical monitoring data, generate reports and trend charts, which is convenient for users to perform data analysis and management. The recorded data includes: sampling time, sampling location, detection results, warning information, etc.

[0017] Compared with the prior art, the beneficial effects of the present invention are:

[0018] 1. In the present invention, the system can realize continuous and on-line monitoring of coliform bacteria in direct drinking water, and timely detect water quality abnormalities. Secondly, by adopting fluorescence quantitative PCR technology, the detection speed is fast, and the detection result can be obtained in a short time for timely warning. At the same time, the pretreatment module processes the water quality to improve the detection sensitivity, so as to meet the requirements of GB19298-2014 standard.

[0019] 2. In the present invention, the whole process from sampling to warning is automated, which greatly reduces manual intervention and operation errors, improves the detection accuracy, and the system can record historical monitoring data through the data management module and perform analysis, providing a basis for water quality management, and thus facilitating the management of data by detection personnel. Description of the Drawings

[0020] Figure 1 The flow of a real-time monitoring and early warning system for coliform bacteria in direct drinking water according to the present invention Figure 1 ;

[0021] Figure 2 It is a flowchart of the operation of a real-time monitoring and early warning system for coliform bacteria in direct drinking water according to the present invention;

[0022] Figure 3 It is a step flowchart of a real-time monitoring and early warning system for coliform bacteria in direct drinking water according to the present invention. Specific embodiments

[0023] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0024] Embodiment

[0025] Please refer to Figure 1 - Figure 3 As shown, the present invention provides a technical solution: a real-time monitoring and early warning system for coliform bacteria in direct drinking water, including a sampling module, the sampling module is signal-connected to a pretreatment module, and the pretreatment module is signal-connected to a detection module. At the same time, the detection module is signal-connected to a control module, the control module is signal-connected to an early warning module, and the early warning module is signal-connected to a data management module;

[0026] In this embodiment, specifically: S1: The system regularly collects water samples and performs pretreatment;

[0027] S2: Specific primers and probes are used to amplify and detect the DNA of coliform bacteria in the water sample, and the change of fluorescence signal is monitored in real time;

[0028] S3: According to the change curve of the fluorescence signal, the threshold cycle number (Ct value) method is used to quantitatively analyze the concentration of coliform bacteria in the water sample;

[0029] S4: The detection result is compared with the limit value specified in the GB19298-2014 standard to determine whether the water quality is qualified;

[0030] S5: When the detection result exceeds the standard, the system triggers an early warning mechanism and issues an early warning signal;

[0031] S6: The system stores data such as detection results and early warning information in the database;

[0032] In this embodiment, specifically, the sampling module includes a micro peristaltic pump and a flow meter, and electrically connects the micro peristaltic pump and the flow meter for automatic collection and quantitative control of water samples. The sampling process is as follows: First, the system automatically starts sampling at a preset frequency (such as once per hour). Then, the peristaltic pump extracts water samples from the drinking water pipeline or the water storage device, and the flow meter ensures quantitative collection.

[0033] In this embodiment, specifically, the pretreatment module performs operations such as filtering and concentrating the water sample. The filtering process is as follows: First, a 5-10 μm stainless steel filter screen is used to remove large particulate impurities. Then, a 0.22 μm microporous membrane (made of polyethersulfone) is used to intercept bacteria and fine particles to ensure the purity of the sample. At the same time, the concentration process can be divided into two types: One: The water sample is centrifuged by a micro centrifuge (rotation speed 3000-5000 rpm, for 5 minutes) to precipitate the bacteria, and the supernatant is discarded, and the sample is concentrated to 1 / 10 of the original volume. Two: Pressurized filtration is performed using an ultrafiltration membrane (cut-off molecular weight 10 kDa) to intercept bacteria and concentrate the sample.

[0034] In this embodiment, specifically, the detection module uses a rapid detection method based on fluorescence quantitative PCR technology to detect coliform bacteria in the water sample.

[0035] Working principle of fluorescence quantitative PCR detection technology: First, primers and probes are designed for the specific genes of coliform bacteria. Then, TaqMan probes or SYBRGreen dyes are used to release fluorescence signals during the PCR amplification process, and the intensity is proportional to the DNA concentration. Finally, the initial DNA concentration is determined by the threshold cycle number (Ct value) - and the smaller the Ct value, the higher the content of coliform bacteria in the sample. The detection process is as follows: First, after the water sample is pretreated, the bacterial cell wall is broken by lysis buffer to release DNA. Then, the prepared water sample DNA is reacted with specific primers, Taq DNA polymerase, dNTPs, and buffer (at a temperature of 95 °C for 3 minutes). Then, fluorescence signals of the water quality are cyclically collected (95 °C for 15 seconds → 60 °C for 1 minute). Finally, the data collected multiple times are used to generate an amplification curve and a standard curve (compared with a standard product of known concentration), and then the content of coliform bacteria in the water quality is obtained.

[0036] In this embodiment, specifically, the control module uses an embedded system or an industrial computer to control, collect, and process data of each module of the system.

[0037] And the water quality calculation formula of the control module is:

[0038] E = 10 (-1 / slope) -1;

[0039] Where:

[0040] E: amplification efficiency (ideal value 1.0, corresponding to 100% efficiency), slope: slope of the standard curve (Ct vs. log concentration);

[0041] In this embodiment, specifically: when the early warning module detects that the coliform bacteria exceed the standard, it issues a warning signal through means such as sound and light alarms and SMS notifications. The sound and light alarm component includes: an LED warning light and a buzzer, and the SMS alarm sends a warning SMS to a preset number through a GSM module (such as SIM800L);

[0042] In this embodiment, specifically: the data management module can record historical monitoring data, generate reports and trend charts, which is convenient for users to perform data analysis and management. At the same time, the data management module can back up data regularly to prevent data loss or damage, ensure the security and integrity of the data, and facilitate subsequent query and analysis.

[0043] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A real-time monitoring and early warning system for coliform bacteria in direct drinking water, including a sampling module, characterized in that: The sampling module is signal-connected to the preprocessing module, and the preprocessing module is signal-connected to the detection module. At the same time, the detection module is signal-connected to the control module, and the control module is signal-connected to the warning module. The warning module is signal-connected to the data management module.

2. The real-time monitoring and early warning system for coliform group in direct drinking water according to claim 1, wherein It includes the following steps: S1: The system regularly collects water samples and performs preprocessing. S2: Specific primers and probes are used to amplify and detect the DNA of coliform bacteria in the water sample, and the change of fluorescence signal is monitored in real time. S3: According to the change curve of the fluorescence signal, the concentration of coliform bacteria in the water sample is quantitatively analyzed by the threshold cycle number (Ct value) method. S4: The test results are compared with the limit values specified in the GB19298-2014 standard to determine whether the water quality is qualified. S5: When the test results exceed the standard, the system triggers an early warning mechanism and issues an early warning signal. S6: The system stores data such as test results and early warning information in the database.

3. The real-time monitoring and early warning system for coliform bacteria in direct drinking water according to claim 2, wherein: The sampling module includes a micro peristaltic pump and a flow meter, and is electrically connected to the micro peristaltic pump and the flow meter for automatic collection and quantitative control of water samples.

4. The real-time monitoring and early warning system for coliform bacteria in direct drinking water according to claim 3, characterized in that: The preprocessing module performs operations such as filtering and concentrating on the water sample.

5. The real-time monitoring and early warning system for coliform bacteria in direct drinking water according to claim 4, characterized in that: The detection module uses a rapid detection method based on fluorescence quantitative PCR technology to detect coliform bacteria in the water sample.

6. The real-time monitoring and early warning system for coliform bacteria in direct drinking water according to claim 5, characterized in that: The control module uses an embedded system or an industrial computer to control, collect, and process data of each module of the system.

7. The real-time monitoring and early warning system for coliform bacteria in direct drinking water according to claim 6, wherein: When the warning module detects that the coliform bacteria exceed the standard, it issues a warning signal by means of sound and light alarm, text message notification, etc.

8. A real-time monitoring and early warning system for coliform bacteria in direct drinking water according to claim 7, characterized in that: The data management module can record historical monitoring data and generate reports and trend charts to facilitate users to perform data analysis and management.

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