50 results about "Methicillin resistance" patented technology

The invention discloses a lyase for killing staphylococcus and an application of the lyase and also discloses an amino acid sequence and an encoding gene sequence of the lyase. A recombined lyase constructed by using the disclosed encoding gene can realize soluble expression in Escherichia coli BL21 (DE3); the lyase can be used for efficiently killing various types of staphylococcus including clinically-separated methicillin-sensitive staphylococcus aureus (MSSA) and methicillin-resistant staphylococcus aureus (MRSA) in vitro and can also be used as an antibiotic for treating staphylococcus infection in vivo; and the disclosed lyase can also be used for rapidly cracking the cell wall of the staphylococcus and releasing substances in cells, such as adenosine triphosphate (ATP), DNA (Deoxyribonucleic Acid) and the like, and various detections on the staphylococcus can be realized through detecting released substances.

The invention relates to a kit for quickly detecting a drug resistance gene of pneumophila pathogenic bacteria, which comprises a gene chip capable of detecting 24 main drug resistance genes of pneumophila pathogenic bacteria and a reaction system for multiple asymmetric PCR reactions. The 24 drug resistance genes are derived from aminoglycosides, quinolones, extended spectrum beta-lactamases (ESBLs), cephalosporins (AmpC), carbapenems and drug resistance genes with vancomycin resistance and methicillin resistance causing membrane permeability transition. The kit can be used for directly detecting drug resistance genes of pneumophila pathogenic bacteria in clinical samples, is simple and quick to operate, does not need culture or drug sensitive tests, gains valuable time for reasonable application of antibiotics to a great extent, and is worthy of clinical popularization and application.

The invention belongs to the technical fields of molecular biological techniques and clinical tests, and particularly discloses a nosocomial infection multiple causative agent parallel detection gene chip and a preparation method and application thereof. The gene chip can be used for rapidly and sensitively detecting fourteen medical treatment infection typical causative agents such as acinetobacter baumannii, enterobacter cloacae, enterococcus faecalis and the like by using one reaction, and the detection data of coinfection of yeast and bacterium which cannot be provided by a traditional amplification method is offered. Furthermore, the gene chip also comprises a methicillin resistance target spot, thus realizing further detection of staphy lococcus infection, therefore, a clinician can realize which causative agent a patient is infected in one or two hours, and realize whether the patient has drug resistance, so as to decide whether the patient needs antibiotics or which antibiotics is needed, so that the gene chip has a greater clinical guiding significance.

Ophthalmic ointments for treating infective eye diseases which are particularly effective against infective eye diseases caused by methicillin-resistant Staphylococcus aureus (MRSA) or methicillin-resistant Staphylococcus epidermidis (MRSE) and contain as the active ingredient from 0.01 to 5.0% of vancomycin hydrochloride. Compared with intravenous administration, topical administration of these ophthalmic ointments is accompanied with no problem of the occurrence of side effects such as renal toxicity and thus enables the maintenance of a therapeutically effective concentration.

The present invention relates to compound for preventing and treating bacterial infection, especially diseases caused by methicillin resisting Staphylococcus aureus, methicillin resisting Staphylococcus epidermidis, Escherichia coli producing extremely-broad spectrum beta-lactamases, Klebsiella pneumoniae and fecal enterococcus producing inactivating enzyme, and its preparation process and medical use.

The invention discloses lyase capable of killing staphylococci and an application of the lyase, which belong to the field of biological agents. The invention discloses an amino acid sequence and a coding gene sequence of the lyase. A pH range of the lyase which plays a role is wide, the activity of cracking the staphylococci is kept within the pH range of 4 to 11, and recombinant protease constructed by adopting coding genes can be solubly expressed in colibacillus BL21(DE3). The lyase can be used for efficiently killing various staphylococci in vitro, including methicillin sensitive staphylococcus aureus and methicillin resistant staphylococcus aureus which are clinically separated, and the lyase can be used as antibiotics for treating staphylococcal infection in vivo. The disclosed lyase can be used for quickly cracking staphylococcal cell walls and releasing substances in cells, such as triphosadenine, DNA and the like, and the staphylococci can be detected by utilizing the released substances.

The present application relates generally to a method and a composition matter that provides a rapid and potent antimicrobial photodynamic inactivation (aPDI) of pathogenic bacteria that express high-affinity cell-surface hemin receptors (CSHRs) using Ga(III)-protoporphyrins IX (GaPpIX or Ga-PpIX). The invention provides an effective treatment option for infections of skin or body cavities that are accessible to visible-light irradiation, such as a handheld LED array emitting visible light (405 nm), especially for infections caused by Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), pathogenic staphylococci, Streptococcus mutans, S. pneumoniae, S. pyogenes, streptococci, corynebacteria, mycobacteria, and Bacillus anthracis.

MRSA CC398 is a clone of S. aureus that has recently emerged in pigs and other domestic animals worldwide. As any other MRSA, the clone displays high levels of antibiotic resistance and poses a serious threat to human health because of the risk of antibiotic treatment failure in human patients. We developed a new diagnostic test for identification of MRSA CC398 using a single one-step PCR that is very easily performed within a few hours. The test is based on the principle that clonal differences within S. aureus are reflected in the sequence of a gene (sau1hsdS1) located on the chromosome of this bacterial species. Accordingly, such a gene represents an optimal target for S. aureus and MRSA identification at the clone level. The test includes detection of the gene conferring methicillin resistance (mecA), therefore allowing rapid discrimination between methicillin-susceptible and methicillin-resistant variants of the clone. A preliminary validation of the test was performed on a collection of CC398 and non-CC398 strains, resulting in 100% sensitivity and 100% specificity. The test can be combined to real-time PCR technology to further reduce simplify the test performance as well as to allow quantification of the target MRSA clone in biological specimens. The invention has important applications related to surveillance and control of MRSA CC398 in humans, animals and food products.

The invention specifically relates to gorgonian-originated fungus secondary metabolite derivatives and application of the same as antiseptics, belonging to the field of medicine. The derivatives provided by the invention exert strong inhibitory effect on methicillin-resistant staphylococcus aureus (MRSA) and vancomycin-resistant enterococcus (VRE) and certain inhibitory effect on Gram-negative bacteria; and the minimal inhibition concentrations MIC of the derivatives are all less than 12.5 mu M, which proves that the derivatives can be developed into potential antibacterial drugs.

The invention discloses a primer for detecting methicillin-resistant staphylococcus aureus. The primer comprises a primer body for amplifying a methicillin-resistant mecA medicine resistant gene, a primer body for amplifying a staphylococcus aureus nuc gene, a fluorescent probe T1 for detecting the methicillin-resistant mecA medicine resistant gene and a fluorescent probe T2 for detecting the staphylococcus aureus nuc gene. The nucleotide sequence is shown as SEQ ID NO:1-6. The invention further discloses a kit comprising the primer for detecting the methicillin-resistant staphylococcus aureus. The primer and the kit are suitable for rapidly detecting methicillin-resistant staphylococcus aureus strains in a suspicious staphylococcus aureus infected person clinic sample, and have the advantages of being high in sensitivity and specificity, stable, timely, convenient to operate and the like.

The invention provides a rapid sample-processing method for preparing hybridization reaction mixtures substantially depleted of RNA, and a method of identifying the methicillin-resistance status and vancomycin-resistance status of an organism.

The invention belongs to the field of medicinal chemistry, and discloses a dialkyl cationic amphiphilic antibacterial peptide analogue with antibacterial activity and a synthesis method thereof. Through four-step reaction, a target product can be simply and fast obtained. A major structure is shown as the accompanying drawing. In-vitro antibacterial activity experiments prove that the most compounds in the series show good antibacterial effects on staphylococcus aureus and escherichia coli; partial compounds show excellent antibacterial activity on super bacteria including MRSA (methicillin-resistance staphylococcus aureus), VRE (vancomycin-resistant enterococcus) and CRE (carbapenemase enterobacteriaceae). In-vitro red cell toxicity experiments also show the series of compounds have low red cell toxicity, so that the series of compounds are hopeful to be used as novel antibacterial candidate medicine. The formulas are shown in the description.

The invention discloses an orixine derivative and an application of orixine derivative in preparation of medicine for resisting drug-fast bacteria, the test shows that the orixine derivative is capable of inhibiting breeding of a plurality of drug-fast bacterium in vitro, and can be used for preparing antibiotic medicines for resisting drug-fast bacteria; the orixine derivative is used for preparing medicines for resisting methicillin-resistant staphylococcus aureus (MRSA), preparing medicines for resisting benzylpenicillin-resistant staphylococcus aureus, and preparing medicines for resisting vancomycin-resistant enterococcus; the medicines can be prepared to the injection, tablet, pill, capsule, a suspending agent or an emulsion for usage, a chemical structural formula of the orixine derivative is shown in formula I, wherein R1,R2 and R3 are defined in a specification.

The present invention relates to compounds and methods of treating, preventing, or lessening the severity of drug-resistant Gram-positive bacterial infections in mammals. The compounds consist of a combination of a gyrase B inhibitor and a protein synthesis inhibitor. These compounds demonstrate antibacterial activity against drug-resistant strains of Gram-positive bacteria, and in particular, methicillin-resistant Staphylococcus aureus (MRSA). Methods for inhibiting the activity of strains of Gram-positive bacteria and methods for treating a bacterial infection caused by such organisms are described herein.

The invention specifically relates to gorgonian-originated fungus secondary metabolite derivatives and application of the same as antiseptics, belonging to the field of medicine. The derivatives provided by the invention exert strong inhibitory effect on methicillin-resistant staphylococcus aureus (MRSA) and vancomycin-resistant enterococcus (VRE) and certain inhibitory effect on Gram-negative bacteria; and the minimal inhibition concentrations MIC of the derivatives are all less than 12.5 mu M, which proves that the derivatives can be developed into potential antibacterial drugs.

The present invention relates to an antibody against a protein specifically expressed in methicillin-resistant strains of Staphylococcus aureus (MRSA), and a method and a kit for detecting MRSA. The present invention enables a fast and accurate detection of MRSA by using both a PBP2a-specific antibody for the detection of PBP2a and a Protein A-specific antibody for the detection of Protein A.

The invention belongs to the pharmaceutical field, relates to beta,beta-dimethyl-acry-lalkannin and an application in preparing medicines for inhibiting drug-resistant bacteria. The invention can provide a natural inhibitor for inhibiting efflux pump multidrug methicillin-resistant staphylococcus aureus. The compound has good inhibiting effect on multidrug staphylococcus aureus resistance strain with NorA efflux pump protein and the antibacterial effect is 13 times of positive contrastive drug norfloxacin. The compound can both inhibit tetracycline-resistant staphylococcus aureus strain Xu212with TetK tetracycline efflux pump protein and macrolide-resistant staphylococcus aureus strain RN4220 with MsrA macrolide efflux pump protein, and the minimal inhibitory concentration is 10.8mu m. The compound of the invention can be prepared to medicines for inhibiting multidrug resistance bacteria and produced to antibacterial injection or external medicine preparation.

The invention relates to the technical field of biotechnology, in particular to a primer probe combination for identification of staphylococcus aureus and drug resistance of staphylococcus aureus. Theprimer probe combination provided by the invention comprises general primer and probe targeting staphylococcus aureus and specific primer and probe targeting methicillin-resistant staphylococcus aureus.The primer and probe has good specificity and can achieve rapid, accurate and sensitive identification targeting SA and MRSA combined with real-time PCR detection method. Experiments show that minimum detection limit of the primer probe for detection of staphylococcus aureus is 3 CF / ml and minimum detection limit of the primer probe for detection of methicillin-resistant staphylococcus aureus is 5 CF / ml.

The present invention relates to a novel antimicrobial compound of lactoquinomycin that is highly effective against many antibiotic-resistant gram-positive bacteria; namely, methicillin-resistant and vancomycin-resistance Staphylococcus aureus, vancomycin-resistant Enterococcus faecilis and Mycobacteria. The present invention also relates to a fermentation process of culturing a Streptomyces strain to prepare the antimicrobial compound and its use in killing the antibiotic-resistant bacteria.

The invention belongs to the technical field of medicines and discloses (1) activity and structures of new meroterpenoid type compounds 1 to 5 derived from a fungus secondary metabolic product, (2) aseparation and purification preparation method and antimicrobial activity of the compounds 1 to 5 and structural formulas of the compounds 1 to 5, and (3) derivative analogues. The compound 1 and thecompound 3 have relatively strong antimicrobial activity on methicillin-resisting staphylococcus aureus, staphylococcus, escherichia coli capable of producing extended-spectrum beta-lactamase, escherichia coli and pseudomonas aeruginosa.

The invention discloses amycolamycin as well as a preparation method thereof and application thereof. The amycolamycin is from a liquid fermentation product of endophytic actinomycetes Amycolatopsis sp.HCa4 in the intestinal tract of asiatic migratory locust, and comprises amycolamycin A or amycolamycin B. According to the experimental research results, the amycolamycin has very strong antibacterial activity on methicillin-resistant staphylococcus epidermidis staphylococcus aureus, has very strong cytotoxic activity on human breast cancer cells M231, can be prepared into a novel anti-tumour lead compound, and overcomes the drug resistance defect of existing antibiotics to a certain extent.

By culturing Lysobacter sp. BMK333-48F3 (deposit number of FERM BP-7477), an antibiotic, tripropeptin Z, tripropeptin A, tripropeptin B, tripropeptin C or tripropeptin D represented by the general formula (I):wherein R is 7-methyl-octyl group, 8-methyl-nonyl group, 9-methyl-dodecyl group, 10-methyl-undecyl group or 11-methyl-dodecyl group, is obtained as antibiotics having excellent antibacterial activities against bacteria and having a novel molecular structure. These tripropeptins each have an excellent antibacterial activity against various bacteria and drug-resistant strains thereof, such as methicillin-resistant strains and vancomycin-resistant strains.

The invention discloses a multifunctional hydrogel and a preparation method thereof, and the multifunctional hydrogel is prepared from an aqueous solution of an alcohol-cationic polymer, an aqueous solution of an aldehyde biomedical polymer and an aqueous solution of 3, 3 '-dithiobis (propionyl hydrazide). The multifunctional hydrogel disclosed by the invention can realize self-healing and rapid hemostasis, has redox responsiveness and relatively low cytotoxicity, has relatively strong antibacterial property on escherichia coli, staphylococcus aureus and methicillin-resistant staphylococcus aureus, can effectively resist a bacterial biofilm, and can be used for quickly stopping bleeding. The hemostatic material is simple in preparation method, low in cost, environment-friendly and low in toxicity, can effectively promote healing of mouse bacterial biofilm infectious skin wounds, is expected to become a novel multifunctional biological material capable of achieving hemostasis, infection resistance and wound healing promotion at the same time, and has a good application prospect in clinical treatment of chronic infectious skin wounds.

Belonging to the technical field of drug research and development, the invention in particular relates to an anti-tumor and antibacterial compound and application thereof. The anti-tumor and antibacterial compound is named as dialboflavusin A or de-Cl-dialboflavusin A. The anti-tumor and antibacterial compounds dialboflavusin A and de-Cl-dialboflavusin A have strong bacteriostatic activity on 7 gram positive bacteria for test: Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and four different methicillin-resistant Staphylococcus aureus strains. The anti-tumor and antibacterial compounds dialboflavusin A and de-Cl-dialboflavusin A have strong inhibiting effect on cervical cancer cell line Hela, human ovarian adenocarcinoma cell line SKOV3, human breast cancer cell line MCF-7, human lung cancer cell line A549, human hepatoma cell line HepG2, human glioma cell line U251 and human gastric carcinoma cell line SGC-7901. The anti-tumor and antibacterial compound provided by the invention can be used for study of anti-tumor active lead compounds or preparation of antibacterial and anti-tumor drugs.

The invention provides a composition used for detection of methicillin-resistant and / or tetracycline-resistant staphylococcus aureus. The composition used for detection of the methicillin-resistant and / or tetracycline-resistant staphylococcus aureus comprises a primer pair shown in SEQ ID No.1 / 2 and at least one of primer pairs shown in SEQ ID NO.3 / 4 and SEQ ID NO.5 / 6; meanwhile, an mPCR-DHPLC / electrophoresis detection kit and method are further constructed on the basis, and pathogenic staphylococcus aureus, MRSA and tetracycline-resistant staphylococcus aureus drug resistant strains in food are detected; and bacterial strain identification is carried out on staphylococcus aureus by utilizing the composition and the method which are disclosed by the invention, and MRSA and a tetracycline-resistant gene can be detected at the same time, so that staphylococcus aureus, MRSA and erythromycin-resistant staphylococcus aureus in a sample can be identified at the same time during one-step detection. The composition used for detection of methicillin-resistant and / or tetracycline-resistant staphylococcus aureus has the advantages of high speed and high throughput; and meanwhile, detection time is short, operation is simple, labour and cost can be greatly saved, and the rapid detection requirement can be met.

A method for diagnosing increased risk of developing Methicillin-resistant Staphylococcus aureus (MRSA) hospital-acquired (HA-MRSA) or community-acquired MRSA (CA-MRSA) which includes obtaining a biological sample from a subject, detecting in the sample a single nucleotide polymorphism (SNP) in the FAM129B gene at position 17 of SEQ ID NO 1, and comparing the nucleotide at position 17 of SEQ ID NO. 1 in the sample with the nucleotide at position 17 in SEQ ID NO. 1, wherein an adenine at position 17 of SEQ ID NO. 1 in the sample indicates an increased risk of developing MRSA or CA-MRSA in the subject.

The invention belongs to the technical field of medicine chemistry, and discloses an aromatic phenol antibacterial peptide simulant with anti-drug resistance activity and no obvious toxicity and a preparation method thereof. The target product is obtained through a reaction of 3 to 4 steps, and the main structure is shown below. Vitro antibacterial activity experiment proves that most of the compounds have good activity on gram-positive bacteria staphylococcus aureus and enterococcus faecalis, gram-negative bacteria large intestine angstrom bacillus and notrophomonas maltophilia, and shows that the compounds have excellent broad-spectrum antibacterial activity; meanwhile, the extracorporeal red blood cell hemolytic data shows that the toxicity is small, and the method has good selectivity.Part of the compounds shows excellent antibacterial activity in superbacteria including methicillin-resistant staphylococcus aureus (MRSA), NDM-1 and KPC-2 enzyme. Therefore, the compounds are expected to be used as a new antibacterial candidate drug.