The invention discloses a high-voltage electrostatic filtering device of virus prevention and control positive-pressure biological protective clothing. The high-voltage electrostatic filtering deviceis characterized by consisting of a fixing belt, a shell, a power supply system, a core filtering assembly, an air supply system and an integrated control circuit, wherein the shell is provided with agas guide pipe, an upper gas valve port butt joint interface and a grounding wire interface, each interface is provided with a sealing ring, the left side and the right side of the shell are each provided with two openings, the air supply system is installed through the four openings to maintain the air volume and the air pressure in the protective clothing, and an airtight pad is used for pressing. According to the invention, the traditional physical barrier filtering principle is broken through, efficient filtration of fine particles and inactivation of pathogenic microorganisms are realized through a high-voltage electrostatic field composite mode while ultra-low pressure drop is maintained; and the filter material of the device provided by the invention has a loose structure, can be repeatedly used after being cleaned, is long in service life and low in replacement frequency, and can be connected with laboratories with three or more levels of organisms or positive-pressure protective clothing worn by high-risk biological pollution external environment operators.