35 results about "Migratory locust" patented technology

The invention provides a biosynthesis inhibitor of migratory locust aggregation pheromone 4-vinyl anisole and application of the biosynthesis inhibitor. The biosynthesis inhibitor of migratory locust aggregation pheromone 4-vinylanisole reduces the activity of 4-vinylphenol methyltransferase, and the 4-vinylphenol methyltransferase contains an amino acid sequence selected from the following sequences: (a) a sequence as shown in any one of SEQ ID No.1 and SEQ ID No.3; and (b) a sequence which is obtained by deleting, inserting and / or substituting one or more amino acid residues in the sequence shown in any one of SEQ ID No.1 and SEQ ID No.3 and has the activity of catalyzing generation of migratory locust aggregation pheromone 4-vinyl anisole. The inhibitor can be applied to biological prevention and control of migratory locusts, can keep locusts in a harmless scattered state instead of killing the locusts, provides a new method for biological prevention and control of migratory locusts, and has a wide application prospect.

The invention relates to dsRNA of miscellaneous migratory locust ribosome large subunit protein genes RPL7 and RPL44 and application of the dsRNA, the dsRNA for synthesizing the miscellaneous migratory locust ribosome large subunit protein genes RPL7 and RPL44 is injected into miscellaneous migratory locust, the RPL7 and RPL44 genes of the miscellaneous migratory locust can be specifically silenced, and the miscellaneous migratory locust can be inhibited from feeding, so that the miscellaneous migratory locust loses nutrition supply and is abnormal in growth and development, and then individual death is caused; the method achieves the purpose of biological prevention and control, has the advantages of specificity, high efficiency and environmental protection on pest prevention and control, is a novel green and pollution-free locust prevention and control technology, and shows a huge application prospect on locust prevention and control.

This invention belongs to the fields of locust biotechnology and gene editing technology, specifically relating to a method for preparing locusts. Crz Methods using gene mutants and their application in locust control. This invention discovers that inhibiting gene mutants in locusts... Crz Gene expression can be used to control locusts. Crz The gene sequence is shown in SEQ ID NO:3. This invention successfully established a homozygous Crz mutant strain of the migratory locust using gene editing technology. It was found that the albino migratory locusts obtained after Crz deletion were more easily preyed upon by natural enemies than the wild-type locusts, exhibited significantly lower levels of the aggregation pheromone 4VA, and showed weaker adaptability to low temperatures compared to the wild type. This provides a new strategy for the biological control of migratory locusts and can also serve as a reference for the biological control of other pests.

The invention discloses a method for regulating and controlling migratory locust embryo diapause by a BTB transcription factor LOCMI00260 and application of the method. According to the present invention, the full length of the BTB transcription factor LOCMI00260 is cloned from migratory locust, the specific double-stranded RNA interference sequence fragment dsRNA of the BTB transcription factor LOCMI00260 is developed, and the dsRNA of the BTB transcription factor LOCMI00260 is introduced into the migratory locust egg by using the microinjection method; the result shows that the migratory locust BTB transcription factor LOCMI00260 can improve the diapause rate of migratory locust. The invention provides a new means and thought for migratory locust prevention and treatment.

The invention relates to application of migratory locust rhythm gene Clock in regulation and control of eclosion rhythm. According to the method, dsRNA of the migratory locust rhythm gene Clock is injected into a migratory locust body cavity, the expression quantity of the migratory locust rhythm gene Clock is remarkably reduced, the circadian rhythm and daily rhythm of migratory locust are both changed by regulating synthesis of 20E, living work and rest formed by long-term coevolution of migratory locust and the external environment are disrupted, serious harm is caused to survival and reproduction of migratory locust, and the migratory locust is prevented from being damaged. And a new way is provided for pest control.

The application discloses an Asian migratory locust MRPL13 gene and application thereof. The nucleotide sequence of the Asian migratory locust MRPL13 gene is shown as SEQ ID NO:1. The nucleotide sequence of the RNA interference fragment of the Asian migratory locust MRPL13 gene is shown as SEQ ID NO:2. The application further provides dsRNA synthesized by the RNA interference fragment of the Asian migratory locust MRPL13 gene. The application of the dsRNA in the prevention and treatment of the Asian migratory locust, RNA interference of the Asian migratory locust, transcription level knockdown of MRPL13, death of the Asian migratory locust larvae and green prevention and control of the Asian migratory locust are realized.

The application belongs to the field of biotechnology and agricultural pest control, and particularly relates to a membrane protein Sec61 alpha gene and application of dsRNA of the membrane protein Sec61 alpha gene in pest control. After cloning and sequencing of the Sec61 alpha gene of Locusta migratoria, the sequence of the Sec61 alpha gene of SEQ ID NO:1 is obtained; then the gene fragment of SEQ ID NO:2 is selected for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the Locusta migratoria, specific Sec61 alpha genes can be silenced, and the Locusta migratoria stops feeding and successively dies before molting. The application provides a new specific molecular target for pest control based on RNA interference, and has very important application value.

This invention belongs to the field of agricultural biotechnology, specifically relating to locusts. LARP4B Genes, their dsRNAs, and applications. To provide novel target genes for green pest control, this invention utilizes bioinformatics methods to obtain genes from the transcriptome of locusts. LAPR4B The gene fragment was further cloned and sequenced, yielding the sequence SEQ ID NO: 1. Based on SEQ ID NO: 1, the dsRNA of this gene was designed and synthesized. The nucleotide sequence of this gene fragment is shown in SEQ ID NO: 2. Injecting it into the locust's body cavity specifically silences the target gene, inhibiting the development of the locust's ovary, resulting in a smaller ovary and shorter, narrower oviducts, thereby effectively suppressing the locust population. Due to the specificity of this invention and its effect on ovarian development, it has significant practical implications for suppressing locust populations and can provide a new approach for pest control.

The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to application of a Rop gene and dsRNA thereof in pest control. The Rop gene of migratory locust is cloned and sequenced to obtain the Rop gene of which the sequence is SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific Rop gene can be silenced, so that the food intake of the migratory locust is obviously reduced, and all migratory locust die before ecdysis. The invention provides a novel specific molecular target for pest control based on RNA interference, and has very important application value.

The application belongs to the field of biotechnology and agricultural pest control, and particularly relates to a membrane protein Sec63 gene and application of dsRNA of the membrane protein Sec63 gene in pest control. After cloning and sequencing of the Sec63 gene of Locusta migratoria, the sequence of the Sec63 gene of SEQ ID NO:1 is obtained; then the gene fragment of SEQ ID NO:2 is selected for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the Locusta migratoria, specific Sec63 genes can be silenced, so that the Locusta migratoria cannot successfully molt and dies when molting. The application provides a new specific molecular target for pest control based on RNA interference and a new technical approach for pest control.

This invention discloses circular RNA circLOCMI02072 (1, 2） Its application as a behavior regulator in locusts falls under the field of agricultural biotechnology. This invention discovers circular RNA. circLOCMI02072 (1, 2） The expression of this circular RNA differs between gregarious and solitary locusts, and its expression level is negatively correlated with locust swarming behavior. Increasing the expression level of this circular RNA in locusts can significantly inhibit locust swarming behavior. This invention provides a novel, green, and pollution-free locust control technology that is highly efficient, specific, and environmentally friendly, and has important application value for locust control.

The application relates to the field of agricultural biotechnology, in particular to an LMSerpinb1 gene and application thereof. A locusta migratoria serpinb1 gene is cloned, and the locusta migratoria mortality and the gene expression amount change of the target gene in the locusta migratoria are detected through the method of mixed feeding of the locusta migratoria with metarhizium anisopliae, so that the role of the locusta migratoria in the metarhizium anisopliae infection is verified, and a foundation for further clarifying the action mechanism of Serpin is laid.

The invention discloses dsRNA of a migratory locust nubbin gene and application of the dsRNA in pest control, and belongs to the technical field of biotechnology and agricultural pest control. The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to application of dsRNA of a nubbin gene in pest control. The method comprises the following steps: cloning and sequencing a nubbin gene of migratory locust to obtain the nubbin gene with a sequence of SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific nubbin gene can be silenced, so that wing development is abnormal after 100% of migratory locust molts, and migratory flight cannot be realized. A novel specific molecular target is provided for pest control based on RNA interference, and a novel technical approach is provided for pest control.

The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to an application of a Sly1 gene and dsRNA thereof in pest control. According to the invention, the Sly1 gene of migratory locust is cloned and sequenced to obtain the Sly1 gene of which the sequence is SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific Sly1 gene can be silenced, so that the food intake of the migratory locust is obviously reduced, and all migratory locust die before ecdysis. The invention provides a novel specific molecular target for pest control based on RNA interference, and has very important application value.

The invention relates to the technical field of biological control, in particular to a method for screening high-toxicity locust pathogenic strains based on carbon and nitrogen metabolism and application of the high-toxicity locust pathogenic strains based on carbon and nitrogen metabolism. And a strain with high toxicity to migratory locust is screened out. ITS (ribosome DNA spacer) sequences of fungi are amplified through ITS1 and ITS4 primers, sequence comparison and phylogenetic analysis are carried out through MEGA 7.0 software, and it is ensured that the strain is metarhizium ledebusii; the method can be used for systematically screening high-toxicity metarhizium ledebusii strains, can be used for biological control of pests, and provides a scientific basis for development of efficient and sustainable pest control strategies.

The invention provides a system and a method for complement expression after migratory locust gene knockout, and belongs to the technical field of insect gene editing, the system for complement expression after migratory locust gene knockout comprises an overexpression plasmid and a living transfection reagent; the overexpression plasmid carries a GAPDH (glyceraldehyde-phosphate dehydrogenase) promoter and a complement gene; the method comprises the following steps: 1) cloning a back-filling gene to a carrier skeleton carrying a GAPDH promoter; 2) respectively dissolving the overexpression plasmid and the living transfection reagent, and then mixing and incubating to obtain a system for complement expression after migratory locust gene knockout; 3, the system is delivered to a migratory locust living body, by means of the system for complement expression after migratory locust gene knockout, the expression quantity of the gene in a migratory locust gene knockout mutant can be specifically complemented, complement expression of the target gene can be detected at the mRNA and protein level, and an effective means is provided for accurately verifying the function of the knockout gene.

The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to an EGFR (epidermal growth factor receptor) gene and application of dsRNA (double-stranded ribonucleic acid) thereof in pest control. The EGFR gene of migratory locust is cloned and sequenced to obtain the EGFR gene with the sequence of SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific EGFR gene can be silenced, 16.7% of migratory locust is difficult to molt, and 83.3% of migratory locust dies due to abnormal wing development after molting. A novel specific molecular target is provided for pest control based on RNA interference, and a novel technical approach is provided for pest control.

The invention relates to the technical field of migratory locust epidermis test probes, in particular to application of a probe to determination of mechanical indexes of fracture displacement corresponding to efficiency, internodal membrane fracture strength and maximum tensile force of a target gene in the insect development process. A migratory locust epidermis testing probe comprises a stretching probe and a puncturing probe, the stretching probe comprises a clamp base, two sets of clamp bases are arranged at the top end of the clamp base oppositely, a knob bolt is rotationally connected to the middle of the right side wall of each clamp base, and springs are arranged on the upper side and the lower side of each knob bolt correspondingly; a plugging screw is mounted on the right side of the spring; the tensile probe is used for measuring mechanical indexes such as the maximum tensile force, the breaking strength and the breaking displacement corresponding to the maximum tensile force of the migratory locust internode membrane under the action of external force, so that people can more intuitively perceive the tensile property of the migratory locust internode membrane; and the maximum force value when the probe penetrates through the sample can be measured through the puncture probe of the texture analyzer, and the specific hardness value is obtained.

This invention provides an injection solution for single-base site-directed editing in locusts and a gene editing method for protein sequence modification using single-base site-directed editing in locusts, belonging to the field of insect gene editing technology. The injection solution comprises the following components at final concentrations: 380-420 ng / μL Cas9, 130-170 ng / μL sgRNA, and 280-320 ng / μL ssODN donor. The injection solution is microinjected into locust eggs, and single-base edited individuals are screened. The method of this invention can specifically alter transcription factors. CAMTA A single base in the protein-coding region of a gene can cause a desired change in the amino acid encoded by the corresponding triplet codon, thereby achieving changes at the protein level from single-base editing. This enables diversified regulation of protein amino acid sequences and functions, providing a new technical approach for locust gene function analysis and pest control research.

The invention relates to a mixing agent for repelling ant pests and application thereof. The mixing agent of diethyl glutarate, 3-methylindole and 2-(formamido) benzoic acid, which is screened and developed from migratory locust reflux liquid, has a relatively good repelling effect on ants, especially solenopsis invicta. Compared with a chemical repellent agent used in the prior art, natural insect defense substances are adopted, higher environmental protection property and safety are achieved, and remarkable market value and wide popularization prospects are achieved.

The utility model discloses a directional trapping device for locusts, including insect box, the upper portion cover of insect box has annular cover, the inside fixed connection of annular cover has conical hopper, the inside of conical hopper is equipped with guide frame, and the periphery of guide frame is fixedly connected with annular cover, and the sliding seal installation of guide frame has food box, and the upper portion of guide frame is equipped with spring, and the upper end of spring is fixedly connected with guide frame, and the lower end of spring is fixedly connected with food box, through conical hopper imports locust into insect box, through the mode of increasing the gap on conical hopper to increase the trapping efficiency, when raining, utilize the water bucket to collect rainwater, utilize the rainwater gravity to downwardly act on the water bucket, downwardly act on the annular sealing cover through the water bucket, downwardly act on the food box through the annular sealing cover, make the food box close down, reduce the gap on conical hopper, reduce the possibility of rainwater into insect box, convenient and compatible trapping efficiency and rainproof.

The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to a transcription factor Grainy head gene and application of dsRNA of the transcription factor Grainy head gene in pest control. According to the invention, the Grainy head gene of migratory locust is cloned and sequenced to obtain the Grainy head gene with a sequence of SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific Grainy head gene can be silenced, so that the migratory locust is difficult to exuviate and die, or the exuviated wings are abnormal in development and cannot fly. The invention provides a novel specific molecular target for pest control based on RNA interference, and has very important application value.

The invention provides an injection for migratory locust single-base fixed-point editing and a migratory locust single-base fixed-point editing gene editing method for protein sequence modification, and belongs to the technical field of insect gene editing. The injection provided by the invention comprises the following components in final concentration: 380 to 420 ng / [mu] L of Cas9, 130 to 170 ng / [mu] L of sgRNA, and 280 to 320 ng / [mu] L of an ssODN donor. The injection is microinjected into locust eggs, and single-base edited individuals are obtained through screening. According to the method disclosed by the invention, a single basic group in a transcription factor CAMTA gene protein coding region can be specifically changed, so that amino acid coded by a corresponding triad codon is subjected to expected change, the change from single basic group editing to a protein level is realized, diversified regulation and control of protein amino acid sequences and functions are realized, and the application prospect is wide. And a new technical approach is provided for migratory locust gene function analysis and pest prevention and control research.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to migratory locust retinoic acid induced protein 1 as well as a coding gene and application thereof. The migratory locust retinoic acid induced protein 1 gene is cloned from migratory locust bodies, primers are designed for the migratory locust retinoic acid induced protein 1 gene, and dsRNA for interfering migratory locust retinoic acid induced protein 1 gene expression is synthesized. An application test result of the migratory locust retinoic acid induced protein 1 gene rai1 in prevention and treatment of migratory locust pests shows that after dsRNA is injected into migratory locust, the egg diapause rate of the migratory locust is remarkably reduced, which indicates that the migratory locust retinoic acid induced protein 1 or the coding gene thereof provided by the invention has the function of regulating and controlling the growth and development of the migratory locust; the dsRNA of the migratory locust retinoic acid-induced protein 1 gene rai1 can reduce the diapause rate of migratory locust eggs, so that migratory locust larvae are hatched in winter in advance, and because the larvae cannot survive in cold weather in winter, the purposes of preventing and controlling migratory locust in generations and realizing biological prevention and control of migratory locust are achieved.

The invention belongs to the technical field of biology, and particularly provides application of a cytochrome P450 enzyme gene in regulating migratory locust embryo diapause. The nucleotide sequence of the cytochrome P450 enzyme gene is as shown in SEQ ID NO: 1. Corresponding dsRNA is designed and synthesized on the basis of SEQ ID NO: 1, then the dsRNA is injected into migratory locust bodies, the expression level of migratory locust cytochrome P450 enzyme genes is reduced, and the diapause rate of migratory locust embryos is reduced. The invention provides a basis for developing novel nucleic acid pesticides and reducing the population quantity of overwintering pests.

The invention discloses an Asian migratory locust MRPL13 gene and application thereof. Wherein the Asian migratory locust MRPL13 gene has a nucleotide sequence as shown in SEQ ID NO: 1. The nucleotide sequence of the RNA interference fragment of the Asian migratory locust MRPL13 gene is as shown in SEQ ID NO: 2. The invention also provides dsRNA synthesized by the RNA interference fragment of the Asian migratory locust MRPL13 gene. According to the application of dsRNA in prevention and control of the Asian migratory locust, RNA interference is performed on the Asian migratory locust, the MRPL13 transcriptional level is knocked down, death of Asian migratory locust larvae is caused, and therefore green prevention and control of the Asian migratory locust are achieved.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and discloses dsRNA of a migratory locust retinoic acid X receptor and application of the dsRNA. The dsRNA of the migratory locust retinoic acid X receptor is specifically dsrxr, and the dsrxr interferes with or inhibits the expression of the migratory locust retinoic acid X receptor gene rxr. The designed and synthesized dsrxr can relieve migratory locust egg diapause and reduce the migratory locust egg diapause rate, and after migratory locust is injected with a dsrxr solution, the migratory locust egg diapause rate is remarkably reduced. After the dsrxr is used, diapause of migratory locust eggs is relieved in the overwintering period, hatched larvae cannot survive in cold climate, and the aim of preventing and controlling migratory locust in generation intervals is achieved.

This invention belongs to the field of agricultural microbiology technology, specifically relating to *Pseudomonas vensuelae* (…). Pseudocitrobacter vendiensis This invention relates to Pv-LC32 and its application in enhancing the pathogenicity of *Metarhizium anisopliae* against locusts. For the first time, this invention isolates a strain of *Pseudomonas vensuelae* from the midgut of locusts, exhibiting conditional synergistic pathogenicity. This strain does not directly kill the host, but significantly enhances fungal infection efficiency through interaction with *Metarhizium anisopliae*. This discovery provides a new perspective on understanding the interaction mechanism between insect gut symbiotic bacteria and pathogenic fungi, and lays a theoretical foundation for developing biocontrol enhancement technologies.

The utility model relates to the field of full-automatic motor sweepers, in particular to a puncture probe for migratory locust epidermis test, which comprises a test platform and a puncture probe, a puncture probe is arranged above the test platform and is formed by combining a probe and a connecting rod, the top end of the probe is fixedly connected with the connecting rod, the outer side of the connecting rod is in threaded connection with the connecting sleeve, and the rear side of the test platform is provided with the lifting column with a lifting groove formed in the surface; a lifting block is movably connected into the lifting groove and fixedly connected to the rear side of the connecting sleeve, an electric push rod is installed at the top end of the lifting column, and the output end of the electric push rod penetrates through the lifting column and is fixedly connected to the lifting table. The electric push rod extends out of the output end to drive the lifting table and the probe to move downwards along the lifting groove and pierce into the epidermis of a sample migratory locust, the electric push rod can accurately apply force and slowly and evenly pressurize, interference of external human factors is not prone to occurring, and accurate data can be obtained.

The invention discloses dsRNA of a migratory locust gene and application of the dsRNA in pest control, and belongs to the technical field of biotechnology and agricultural pest control. The method comprises the following steps of: cloning and sequencing an extraneous gene of migratory locust, so as to obtain the extraneous gene of which the sequence is SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, a specific extraneous gene can be silenced, so that 83.6% of migratory locust is difficult to exuviate and die, and 16.4% of migratory locust is abnormal in wing development and cannot migrate after exuviating. A novel specific molecular target is provided for pest control based on RNA interference, and a novel technical approach is provided for pest control.