15 results about "Migratory locust" patented technology

This invention belongs to the fields of locust biotechnology and gene editing technology, specifically relating to a method for preparing locusts. Crz Methods using gene mutants and their application in locust control. This invention discovers that inhibiting gene mutants in locusts... Crz Gene expression can be used to control locusts. Crz The gene sequence is shown in SEQ ID NO:3. This invention successfully established a homozygous Crz mutant strain of the migratory locust using gene editing technology. It was found that the albino migratory locusts obtained after Crz deletion were more easily preyed upon by natural enemies than the wild-type locusts, exhibited significantly lower levels of the aggregation pheromone 4VA, and showed weaker adaptability to low temperatures compared to the wild type. This provides a new strategy for the biological control of migratory locusts and can also serve as a reference for the biological control of other pests.

The application discloses an Asian migratory locust MRPL13 gene and application thereof. The nucleotide sequence of the Asian migratory locust MRPL13 gene is shown as SEQ ID NO:1. The nucleotide sequence of the RNA interference fragment of the Asian migratory locust MRPL13 gene is shown as SEQ ID NO:2. The application further provides dsRNA synthesized by the RNA interference fragment of the Asian migratory locust MRPL13 gene. The application of the dsRNA in the prevention and treatment of the Asian migratory locust, RNA interference of the Asian migratory locust, transcription level knockdown of MRPL13, death of the Asian migratory locust larvae and green prevention and control of the Asian migratory locust are realized.

The application belongs to the field of biotechnology and agricultural pest control, and particularly relates to a membrane protein Sec61 alpha gene and application of dsRNA of the membrane protein Sec61 alpha gene in pest control. After cloning and sequencing of the Sec61 alpha gene of Locusta migratoria, the sequence of the Sec61 alpha gene of SEQ ID NO:1 is obtained; then the gene fragment of SEQ ID NO:2 is selected for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the Locusta migratoria, specific Sec61 alpha genes can be silenced, and the Locusta migratoria stops feeding and successively dies before molting. The application provides a new specific molecular target for pest control based on RNA interference, and has very important application value.

This invention belongs to the field of agricultural biotechnology, specifically relating to locusts. LARP4B Genes, their dsRNAs, and applications. To provide novel target genes for green pest control, this invention utilizes bioinformatics methods to obtain genes from the transcriptome of locusts. LAPR4B The gene fragment was further cloned and sequenced, yielding the sequence SEQ ID NO: 1. Based on SEQ ID NO: 1, the dsRNA of this gene was designed and synthesized. The nucleotide sequence of this gene fragment is shown in SEQ ID NO: 2. Injecting it into the locust's body cavity specifically silences the target gene, inhibiting the development of the locust's ovary, resulting in a smaller ovary and shorter, narrower oviducts, thereby effectively suppressing the locust population. Due to the specificity of this invention and its effect on ovarian development, it has significant practical implications for suppressing locust populations and can provide a new approach for pest control.

This invention discloses circular RNA circLOCMI02072 (1, 2） Its application as a behavior regulator in locusts falls under the field of agricultural biotechnology. This invention discovers circular RNA. circLOCMI02072 (1, 2） The expression of this circular RNA differs between gregarious and solitary locusts, and its expression level is negatively correlated with locust swarming behavior. Increasing the expression level of this circular RNA in locusts can significantly inhibit locust swarming behavior. This invention provides a novel, green, and pollution-free locust control technology that is highly efficient, specific, and environmentally friendly, and has important application value for locust control.

The invention discloses dsRNA of a migratory locust nubbin gene and application of the dsRNA in pest control, and belongs to the technical field of biotechnology and agricultural pest control. The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to application of dsRNA of a nubbin gene in pest control. The method comprises the following steps: cloning and sequencing a nubbin gene of migratory locust to obtain the nubbin gene with a sequence of SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific nubbin gene can be silenced, so that wing development is abnormal after 100% of migratory locust molts, and migratory flight cannot be realized. A novel specific molecular target is provided for pest control based on RNA interference, and a novel technical approach is provided for pest control.

The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to an EGFR (epidermal growth factor receptor) gene and application of dsRNA (double-stranded ribonucleic acid) thereof in pest control. The EGFR gene of migratory locust is cloned and sequenced to obtain the EGFR gene with the sequence of SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific EGFR gene can be silenced, 16.7% of migratory locust is difficult to molt, and 83.3% of migratory locust dies due to abnormal wing development after molting. A novel specific molecular target is provided for pest control based on RNA interference, and a novel technical approach is provided for pest control.

This invention provides an injection solution for single-base site-directed editing in locusts and a gene editing method for protein sequence modification using single-base site-directed editing in locusts, belonging to the field of insect gene editing technology. The injection solution comprises the following components at final concentrations: 380-420 ng / μL Cas9, 130-170 ng / μL sgRNA, and 280-320 ng / μL ssODN donor. The injection solution is microinjected into locust eggs, and single-base edited individuals are screened. The method of this invention can specifically alter transcription factors. CAMTA A single base in the protein-coding region of a gene can cause a desired change in the amino acid encoded by the corresponding triplet codon, thereby achieving changes at the protein level from single-base editing. This enables diversified regulation of protein amino acid sequences and functions, providing a new technical approach for locust gene function analysis and pest control research.

The utility model discloses a directional trapping device for locusts, including insect box, the upper portion cover of insect box has annular cover, the inside fixed connection of annular cover has conical hopper, the inside of conical hopper is equipped with guide frame, and the periphery of guide frame is fixedly connected with annular cover, and the sliding seal installation of guide frame has food box, and the upper portion of guide frame is equipped with spring, and the upper end of spring is fixedly connected with guide frame, and the lower end of spring is fixedly connected with food box, through conical hopper imports locust into insect box, through the mode of increasing the gap on conical hopper to increase the trapping efficiency, when raining, utilize the water bucket to collect rainwater, utilize the rainwater gravity to downwardly act on the water bucket, downwardly act on the annular sealing cover through the water bucket, downwardly act on the food box through the annular sealing cover, make the food box close down, reduce the gap on conical hopper, reduce the possibility of rainwater into insect box, convenient and compatible trapping efficiency and rainproof.

The invention belongs to the technical field of biology, and particularly provides application of a cytochrome P450 enzyme gene in regulating migratory locust embryo diapause. The nucleotide sequence of the cytochrome P450 enzyme gene is as shown in SEQ ID NO: 1. Corresponding dsRNA is designed and synthesized on the basis of SEQ ID NO: 1, then the dsRNA is injected into migratory locust bodies, the expression level of migratory locust cytochrome P450 enzyme genes is reduced, and the diapause rate of migratory locust embryos is reduced. The invention provides a basis for developing novel nucleic acid pesticides and reducing the population quantity of overwintering pests.

This invention belongs to the field of agricultural microbiology technology, specifically relating to *Pseudomonas vensuelae* (…). Pseudocitrobacter vendiensis This invention relates to Pv-LC32 and its application in enhancing the pathogenicity of *Metarhizium anisopliae* against locusts. For the first time, this invention isolates a strain of *Pseudomonas vensuelae* from the midgut of locusts, exhibiting conditional synergistic pathogenicity. This strain does not directly kill the host, but significantly enhances fungal infection efficiency through interaction with *Metarhizium anisopliae*. This discovery provides a new perspective on understanding the interaction mechanism between insect gut symbiotic bacteria and pathogenic fungi, and lays a theoretical foundation for developing biocontrol enhancement technologies.

The utility model relates to the field of full-automatic motor sweepers, in particular to a puncture probe for migratory locust epidermis test, which comprises a test platform and a puncture probe, a puncture probe is arranged above the test platform and is formed by combining a probe and a connecting rod, the top end of the probe is fixedly connected with the connecting rod, the outer side of the connecting rod is in threaded connection with the connecting sleeve, and the rear side of the test platform is provided with the lifting column with a lifting groove formed in the surface; a lifting block is movably connected into the lifting groove and fixedly connected to the rear side of the connecting sleeve, an electric push rod is installed at the top end of the lifting column, and the output end of the electric push rod penetrates through the lifting column and is fixedly connected to the lifting table. The electric push rod extends out of the output end to drive the lifting table and the probe to move downwards along the lifting groove and pierce into the epidermis of a sample migratory locust, the electric push rod can accurately apply force and slowly and evenly pressurize, interference of external human factors is not prone to occurring, and accurate data can be obtained.

The invention discloses dsRNA of a migratory locust gene and application of the dsRNA in pest control, and belongs to the technical field of biotechnology and agricultural pest control. The method comprises the following steps of: cloning and sequencing an extraneous gene of migratory locust, so as to obtain the extraneous gene of which the sequence is SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, a specific extraneous gene can be silenced, so that 83.6% of migratory locust is difficult to exuviate and die, and 16.4% of migratory locust is abnormal in wing development and cannot migrate after exuviating. A novel specific molecular target is provided for pest control based on RNA interference, and a novel technical approach is provided for pest control.

The present application relates to the technical field of pest control, and further provides a method for extracting and identifying locust sex pheromone, which comprises the following steps: extracting locust volatile matter, and identifying the main components of the volatile matter as (Z)-2-pentene-1-ol, benzaldehyde, 2-ethyl-1-hexanol, 3-ethyl-benzaldehyde, 2-hexyl-1-dodecanol, 2-methoxyphenol, 4-ethyl-acetophenone, butyl benzoate, phenol, 2-ethyl-4,5-dimethylphenol, dimethyl phthalate, 2,4-di-tert-butylphenol, methyl hexanoate, isooctyl phthalate and dibutyl phthalate. Experiments show that dibutyl phthalate (DBP) as locust sex pheromone has a good attraction to male locusts, and can be applied to the biological control of locusts. The present application provides a new method for the biological control of locusts, and has a wide application prospect.

The present application relates to a kind of mixed agent for repelling ant pests and its application, the mixed agent of glutaric acid diethyl ester, 3-methylindole and 2-(formylamino) benzoic acid screened and developed from locust reverse flow liquid has good repelling effect to ant, especially red imported fire ant.Compared with the chemical repellent used in prior art, the present application uses insect natural defense material, has higher environmental protection and safety, has significant market value and broad popularization prospect.