10results about How to "Strong pathogenicity" patented technology

This invention provides a highly pathogenic feline calicivirus strain, its vaccine, and its applications. The highly pathogenic feline calicivirus was isolated from pathogenic material and identified as a highly pathogenic strain, named FCV CC475, with its cDNA sequence shown in SEQ ID NO.1. This strain exhibits strong pathogenicity, causing infected animals to develop symptoms such as fever, paw pad ulcers, and dehydration by day 3 post-infection; the viral load after 5 generations is 10. 9.80 TCID 50 / ml. This strain produces high antibody titers after immunization of cats and rabbits, demonstrating good immunogenicity. Therefore, this highly pathogenic feline calicivirus has broad application prospects in the preparation of feline calicivirus infection vaccines, diagnostic reagents, or therapeutic drugs.

The invention discloses a canine parvovirus CPV-2c subtype virus strain and application thereof, and relates to the technical field of biology. The canine parvovirus CPV-2c subtype virus strain is preserved in the China Center for Type Culture Collection on November 12, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: V202492. Genetic evolution analysis shows that the canine parvovirus CPV-2c subtype virus strain obtained through separation has a better genetic relationship with other epidemic strains published domestically. The strain has good pathogenicity, can show typical CPV clinical symptoms when infected dogs, has great advantages for verifying therapeutic drugs and evaluating vaccine efficacy in vivo, and plays an important role in research and development of the therapeutic drugs and vaccines. In addition, the strain can be cultured and proliferated in vitro and has the potential of being developed into vaccines.

The application discloses a kind of Gliocephalotrichum simmonsii and application thereof, the strain number of the Gliocephalotrichum simmonsii (Gliocephalotrichum simmonsii) is JSAFC 2056, and is preserved in China Microorganism Culture Collection Center on September 23, 2024, and the preservation number is CGMCC No.41562.The biocontrol agent of the application provided can significantly inhibit the survival activity of Anomala cuprea, and the survival activity of Anomala cuprea can be reduced to 0 after 28 days of treatment, and the biocontrol agent is environmentally friendly, non-toxic and harmless.The preparation method of the biocontrol agent of the application provided is simple, the culture requirement is low, and has good application prospect.

The present application relates to the animal medical technology field, especially to the Streptococcus suis strain and the vaccine thereof for prevention and treatment, and provides Streptococcus suis type 2, type 3 and type 9 strains screened and obtained, and a trivalent inactivated vaccine prepared by mixing the three strains, which can safely and effectively prevent and protect against the attack of the three pathogenic bacteria of Streptococcus suis.

The invention provides a porcine rotavirus G5 type and application thereof. According to the present invention, the porcine rotavirus G5 type SC05 strain is separated from the porcine intestinal tract tissue, and is subjected to passage purification to obtain the porcine rotavirus G5 type SC05 strain, and the microbial preservation number of the porcine rotavirus G5 type SC05 strain is CGMCC No.46179; the isolated strain can stably proliferate on passage cells to generate typical cytopathy. The G5 type isolated strain of the porcine rotavirus disclosed by the invention has excellent immunogenicity; the vaccine prepared from the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, the ELISA kit prepared from the isolated strain can be used for well detecting the porcine rotavirus G5 type antibody in serum.

The invention discloses a beauveria bassiana strain for preventing and treating caffeinia and application of the beauveria bassiana strain, and belongs to the technical field of biological prevention and treatment of pests. The strain disclosed by the invention is Beauveria bassiana which is separated from a larva body of the caffeine calandria for the first time, the Beauveria bassiana is biologically preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.40829. The invention further discloses a preparation method of the Beauveria bassiana. The strain has the advantages of being simple in culture, large in sporulation quantity and high in pathogenicity to larvae of the caffeine, and is suitable for being used for developing a biological preparation for preventing and treating the pest.

This invention discloses a strain of *Backusella circina* and its application. The strain number of *Backusella circina* is JSAFC 2057, which was deposited at the China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, with accession number CGMCC No. 41522. This fungal agent can significantly inhibit the survival activity of the fall webworm (*Triplophysa fulvidraco*), reducing the survival activity of fall webworm larvae to zero after 7 days of treatment and culture. The fungus provided by this invention is a biocontrol fungus for the control of the major pest, the fall webworm. It is characterized by strong pathogenicity against fall webworm larvae, environmental safety, and low likelihood of inducing pesticide resistance in pests, and can be applied to the biological control of the fall webworm.

This invention provides the application of the FgFd1 gene in regulating the growth, reproduction, pathogenicity, and stress resistance of *Fusarium graminearum*, belonging to the field of genetic engineering technology. The nucleotide sequence of the FgFd1 gene is shown in SEQ ID NO.2, and the amino acid sequence of the protein encoded by the FgFd1 gene is shown in SEQ ID NO.1. The *Fusarium graminearum* FgFd1 gene knockout mutant (ΔFgFd1) obtained by this invention exhibits slower growth rate, reduced conidia number, decreased pathogenicity, and reduced stress resistance compared to the wild-type (WT) strain. This invention provides a technical basis for exploring the molecular mechanisms of sporulation and pathogenicity of *Fusarium graminearum*, and provides potential molecular targets for developing disease control measures targeting sporulation inhibition, showing broad application prospects in plant fungal disease research.

The application discloses an anopheles elizabethkingia JX2021 strain from Chinese soft-shelled turtles, and the anopheles elizabethkingia JX2021 strain has been preserved in the China Center for Type Culture Collection (Wuhan) on April 28, 2022, and the preservation number is CCTCC NO: M 2022507. The anopheles elizabethkingia JX2021 strain has strong pathogenicity to Chinese soft-shelled turtles, can cause different degrees of lesions in tissues such as liver, spleen, lung and intestinal tract of the Chinese soft-shelled turtles, causes bacterial septicemia of the Chinese soft-shelled turtles, and can even cause death. The anopheles elizabethkingia JX2021 strain can also be used for breeding of Chinese soft-shelled turtle seedlings against the anopheles elizabethkingia, construction of an animal model, and preparation of drugs and vaccines.

The invention discloses a broad bean red spot pathogenic bacterium laboratory spore production method, and belongs to the field of plant protection. The method comprises the following two steps: firstly, carrying out low-temperature stress treatment, putting a sterilized wet carrier into a culture dish, then putting hypha blocks or mature sclerotia of pathogenic bacteria of the red spot disease of the broad beans, freezing for 0.5-1 day in a dark place at the temperature of-25 DEG C to-10 DEG C, and then culturing for 3-5 days at the constant temperature of 15-25 DEG C; and then carrying out variable-temperature alternate induction culture, alternately culturing the treated material under the constant-temperature conditions of 9-19 DEG C and 2-8 DEG C for 12 hours, culturing hypha blocks for 5-7 days, and culturing mature sclerotia for 9-11 days until conidiophores and conidia can be seen by naked eyes. The hypha blocks or sclerotium are prepared by activation culture of a PDA culture medium, and the wetting carrier is filter paper, gauze or sponge which is soaked with sterile distilled water after sterilization. Efficient and stable spore production is achieved through low-temperature stress treatment and variable-temperature alternate induction culture, a key material is provided for related scientific research and green prevention and control of the broad bean red spot disease, and the method has important practical value.