The invention relates to a biological fermentation feed and a preparation method thereof, belonging to the field of solid feeds. The biological fermentation feed provided by the invention is prepared from a compound bacteria solution and a fermentation substrate through fermentation, wherein the compound bacteria solution comprises bacillus subtilis, composite saccharomycetes, lactic acid bacillus and bifidobacterium species; the fermentation substrate comprises the following components in parts by weight: 30-35 parts of corns, 10-15 parts of soya bean meal, 25-35 parts of rice washing bran, 10-15 parts of wheat bran, 3-8 parts of yeast extract powder, 0.5-2 parts of sodium chloride, 1-5 parts of calcium hydrophosphate, 0-3 parts of soybean oil, 0.5-1.5 parts of manganese sulfate and 0.5-1.5 parts of magnesium sulfate. The biological fermentation feed provided by the invention is free from antibiotics, is green and safe, is prepared from the raw materials with wide sources, is large in maneuverability, has remarkable benefits, and is capable of reducing morbidity of various diseases.