21 results about "Fall webworm" patented technology

The invention discloses a production process for culturing nuclear polyhedrosis virus by using an S2 or SF9 cell line. The preparation method comprises the following steps: connecting a cell-penetrating peptide C105Y with DSPE-PEG2000-Mal on the surface of a lipid nanoparticle, then preparing a lipid nanoparticle LNP-C105Y by using the DSPE-PEG2000-Mal, and entrapping nuclear polyhedrosis virus DNA to obtain DNAHcNPV LNP-C105Y; the DNAHcNPV coated LNP-C105Y is inoculated into an SF9 grassland spodoptera frugiperda cell line or an S2 fruit fly cell line to be cultured, and the nuclear polyhedrosis virus is produced. On the basis of the fall webworm nuclear polyhedrosis virus, DNAHcNPV-LNP-C105Y lipid nanoparticles are synthesized and prepared by adopting a film dispersion-extrusion method to wrap the fall webworm nuclear polyhedrosis virus, and the fall webworm nuclear polyhedrosis virus is produced by inoculating the fall webworm nuclear polyhedrosis virus to SF9 and S2 cell lines. The hyphantria cunea nuclear polyhedrosis virus production process is improved, and the defect that the hyphantria cunea nuclear polyhedrosis virus production process in the prior art cannot be separated from hyphantria cunea living body feeding is overcome.

The invention belongs to the field of fall webworm control, and provides a fall webworm control dual-trapping and parasitic wasp releasing multifunctional device which comprises a funnel, a groove is formed in the side wall of the funnel and concaves inwards, a plurality of evenly-distributed leakage holes are formed in the side wall of the groove, and a mounting plate is fixedly mounted on the peripheral side face of the funnel. A collecting barrel is movably mounted on the lower side of the mounting plate, the funnel is communicated with the interior of the collecting barrel, and a small bee cocoon placer is movably mounted on the lower side of the collecting barrel; the device is upgraded on the basis of an original fall webworm square trap, a novel Chouioia cunea cocoon placing device is specifically transformed on the lower half part, external wind and rain invasion is blocked, damage to Chouioia cunea cocoons is relieved, the bee output rate of Chouioia cunea is increased, operation is simpler and more convenient, and the device is suitable for popularization and application. According to the upper half part, a square-barrel-shaped trap is modified, a solar LED lamp and sex pheromone are combined for trapping, cost is reduced, and larvae, pupae and adults of the white moths can be prevented and controlled in all stages.

The invention relates to a method for cultivating insect-resistant transgenic Xiaoblack poplar, in particular to a method for cultivating insect-resistant transgenic Xiaoblack poplar. The invention aims to solve the problem of low insect resistance of the populus similis. The cultivation method comprises the following steps: 1, synthesizing a Cry1Ac gene, wherein the nucleotide sequence of the gene is shown as Seq ID No: 1 in a sequence table; 2, a plant expression vector pBI121-Cry1Ac expressed by the Cry1Ac gene is constructed, and the plant expression vector pBI121-Cry1Ac is constructed; 3, preparing an infection solution; 4, cultivating the Cry1Ac-transgenic small black poplar by using an agrobacterium-mediated leaf disc transformation method, the Cry1Ac-transgenic poplar is created, the average concentration of leaf Cry1Ac protein is more than 200ng / g, the insect resistance of the transgenic poplar can be improved, all fall webworms die after being fed for 5 days, and the corrected death rate reaches 100%. The method is applied to the field of molecular breeding.

The utility model discloses adjustable white moth video monitoring equipment, which comprises a moth luring barrel, a bottom barrel seat, a connecting circular ring in split combined connection with the bottom barrel seat, a top barrel cover connected with the top end of the connecting circular ring, a vertical rod of which the bottom end is connected with the top barrel cover, and a shielding cover connected with the top end of the vertical rod, the solar bracket comprises a supporting plate mounted on the shielding cover, a solar panel mounted on the inclined surface of the supporting plate, and a connecting plate mounted on the bottom surface of the middle position; a white moth video camera mechanism; the trapper is provided with a video camera for trapping the number of adults, an ultraviolet lamp emits ultraviolet rays and attracts moths, the moths are prevented from flying out of the trapping barrel, a motor is driven to rotate forwards and backwards, the position of a miniature video monitoring camera is adjusted, and the number of the adults trapped in the camera bottom barrel base can be monitored at different angles. When the miniature video monitoring camera is turned on, shooting can be carried out and the number of moths can be monitored.

The utility model discloses a hyphantria cunea situation monitoring device, and relates to the technical field of hyphantria cunea monitoring. The fall webworm pest situation monitoring device comprises a base, a shell is fixedly installed on the upper surface of the base, the top of the shell is communicated with a vertical pipe, the top of the vertical pipe is communicated with a funnel, a box body is inserted into one side of the shell, a positioning block is fixedly installed on one side of the box body, and baffles are hinged to the two sides of the inner wall of the box body through hinges; according to the utility model, the stop block is pulled, then the insertion rod on the stop block is separated from the upper surface of the base, and then the bottom of the insertion rod is separated from the upper surface of the base, so that the positioning block can be conveniently canceled in the subsequent use process, and the box body on the positioning block can be conveniently separated; the device can be conveniently detached in the subsequent use process, the use convenience of the device is improved, subsequent use is facilitated, insects in the box body can be conveniently taken out in the subsequent process, and subsequent monitoring is facilitated.

The invention provides an insecticide based on nano-copper and application of the insecticide in prevention and treatment of agricultural and forestry diseases and pests, and belongs to the field of nano-material preparation technology and agricultural and forestry disease and pest prevention and treatment. The invention aims to solve the problems of residue pollution and pest resistance of the traditional chemical insecticide at present, and prepares nano-copper particles by co-culturing bacillus amyloliquefaciens and copper nitrate, and the main component of the nano-copper particles is Cu4O3. By disturbing the feeding capacity and nutrient absorption of fall webworms, efficient killing of target pests is achieved, and the median lethal concentration (LC50) is 15.77 mg / g. The method is environment-friendly, efficient, low in cost and simple to operate, provides a new thought for sustainable control of agricultural and forestry pests, and is suitable for popularization and application.

The invention relates to the technical field of prevention and control of agricultural and forestry pests, and discloses a synergistic protein ORF76 of insect baculovirus and application of the synergistic protein ORF76. According to the invention, a synergistic protein (ORF76) is obtained from an insect biocontrol resource, i.e., a Pinus fumosa nuclear polyhedrosis virus, and protein function verification shows that the protein has a remarkable synergistic effect on a fall webworm nuclear polyhedrosis virus, an apocheima cinerarius nuclear polyhedrosis virus and a Pinus fumosa nuclear polyhedrosis virus; the compound can be used as a synergistic factor to be added into insect viruses, and has important significance on prevention and control of major forestry pests (fall webworms, spring inchworm and smoke wing pine bees).

This invention discloses an antimicrobial peptide for controlling the fall webworm. Hcattacin1 Gene dsRNA and its application in the control of the American white moth. Hcattacin1 The gene's nucleotide sequence is SEQ ID NO: 1. This invention is based on antimicrobial peptides. Hcattacin1 dsRNA was genetically engineered, and a method for controlling the fall webworm using injection was established. Hcattacin1 An RNA interference system was used, and it was found that injection into the fall webworm (American white moth) achieved a significant gene silencing effect. Furthermore, the fall webworm was used in conjunction with other RNA interference methods. Hcattacin1 The combined use of dsRNA and biocontrol bacteria can significantly improve the control efficacy of biocontrol bacteria. This invention lays the theoretical foundation for the development of immunosuppressants that are safe for humans and animals, provides a new theory for the biological control of pests, and ultimately offers new strategies and approaches for pest control in my country's green agricultural production and sustainable development.

The invention relates to the technical field of pest control, in particular to dsRNA of hyphantria cunea detoxification metabolism genes CYP6B2 and CYP4G15 and application of the dsRNA and terpine-4-ol in control of hyphantria cunea. The dsRNA of the hyphantria cunea detoxification metabolism gene CYP6B2 is obtained by synthesizing a nucleotide sequence as shown in SEQ ID No.1 and a reverse complementary sequence; the dsRNA of the hyphantria cunea detoxification metabolism gene CYP4G15 is synthesized from a nucleotide sequence as shown in SEQ ID No.3 and a reverse complementary sequence as shown in SEQ ID No.3. By adopting the dsRNA provided by the invention, the sensitivity of the fall webworm to terpin-4-ol can be improved, and meanwhile, the death rate of the fall webworm can be remarkably improved after the dsRNA is combined with terpin-4-ol. Meanwhile, the technical bottleneck that a botanical insecticide has a poor lethal effect on the fall webworm can be solved, and a new thought and strategy are provided for green and efficient prevention and treatment of the fall webworm.

The utility model discloses an aerial spraying device for preventing and controlling fall webworms, which comprises a flying component, a spraying box is mounted at the bottom of the flying component, a spraying shell is arranged below the spraying box, and an atomizing nozzle is connected to the bottom of the spraying shell in a pluggable manner. The spraying box, the spraying shell, the spraying nozzle, the center plate, the mounting plate, a motor, flying blades, an electric pump, a through opening plate, a clamping groove, a clamping block, a dismounting opening, a transverse plate, a vertical plate, a stirring shaft, a stirring rod, a rotating shaft, a wind receiving blade, an inserting block, a stabilizing plate, a limiting plate, a limiting rod, a pulling plate and a spring are matched with one another; a worker can conveniently and quickly disassemble and assemble the atomizing spray head, so that the atomizing spray head can be conveniently maintained, repaired or replaced, the workload of the worker is relieved, liquid medicine in the atomizing box can be stirred under the action of wind force generated when the spraying device flies in the air, effective substances in the liquid medicine are effectively prevented from being precipitated, and the spraying effect is improved. The use is convenient.

The utility model provides an aerial spraying device for fall webworm prevention and control, and relates to the field of spraying equipment for fall webworm prevention and control, the aerial spraying device comprises a movable chassis, a spraying assembly is installed at the top of the movable chassis, the spraying assembly comprises a pesticide box, and a stirring assembly is installed on the surface of the pesticide box. The stirring assembly comprises a motor, a speed regulator, a stirring shaft, stirring blades and a stirring rod, the motor and the speed regulator are both fixed to the top of the pesticide box, and the stirring shaft, the stirring blades and the stirring rod are all installed in an inner cavity of the pesticide box; according to the forest tree pesticide spraying device, the pesticide box, the conveying pump, the flow dividing pipe and the spraying head are arranged, the effect of spraying pesticide to forest trees is achieved, the motor, the speed regulator, the stirring shaft, stirring blades and stirring rods are matched, the pesticide in an inner cavity of the pesticide box can be stirred, and therefore the situation that the pesticide precipitates in the inner cavity of the pesticide box can be prevented; the use effect of the pesticide is effectively improved, and the control effect of the white moth is further improved.

The utility model discloses an efficient hyphantria cunea trapping device, and relates to the technical field of trapping devices. The efficient fall webworm trapping device comprises a trapping cylinder, and the trapping cylinder comprises an upper cylinder body, a lower cylinder body and a connecting cylinder body; the trapping assembly comprises a mounting column arranged in the trapping cylinder and a plurality of fall webworm sex attractant lure cores arranged on the outer surface of the mounting column, a plurality of inclined upward insect inlet pipes extending to the outer surface of the connecting cylinder are embedded in the outer surface of the connecting cylinder in a circumferential array mode, and the top ends of the insect inlet pipes are of a conical tubular structure. According to the hyphantria cunea sex attractant trapping device, by arranging the trapping assembly and the collecting assembly, the defect that in the prior art, the trapping efficiency is low is effectively overcome, the purpose of rapidly replacing the hyphantria cunea sex attractant trapping core can be achieved, meanwhile, the purpose of rapidly treating the corpses of hyphantria cunea can be achieved, and therefore the hyphantria cunea sex attractant trapping device is suitable for popularization and application. The defect that in the prior art, the hyphantria cunea corpse is inconvenient to clean is overcome.

This invention relates to a HcKynU Genes, dsRNAs targeting these genes, and their applications. This invention focuses on horizontal gene transfer. HcKynU Designing dsRNAs specifically for the control of the fall webworm (Russian white moth) not only allows for precise pest control but also avoids threatening non-target species. Injecting this dsRNA into the fall webworm effectively silences its target gene, leading to increased mortality and impaired normal growth and development, ultimately achieving control. This invention provides a new and effective approach for controlling the fall webworm.

The invention relates to the technical field of biological prevention and control, and particularly discloses a method for field prevention and control of fall webworms by using brachycera pubescens, which comprises the following steps: collecting and domesticating brachycera pubescens species, raising hosts in a standardized manner, propagating brachycera pubescens, and regularly rejuvenating populations. And releasing the hyphantria cunea in a pupal stage at a density of 50-250 per mu under a suitable environmental condition. According to the method provided by the invention, by optimizing the bee pupae ratio and the field release density, the field parasitism rate of the brachycera majorana is remarkably increased, the highest parasitism rate can reach 62.5% and is far higher than the natural parasitism rate, and a new effective way is provided for green and sustainable prevention and control of fall webworms.

This invention discloses a strain of *Backusella circina* and its application. The strain number of *Backusella circina* is JSAFC 2057, which was deposited at the China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, with accession number CGMCC No. 41522. This fungal agent can significantly inhibit the survival activity of the fall webworm (*Triplophysa fulvidraco*), reducing the survival activity of fall webworm larvae to zero after 7 days of treatment and culture. The fungus provided by this invention is a biocontrol fungus for the control of the major pest, the fall webworm. It is characterized by strong pathogenicity against fall webworm larvae, environmental safety, and low likelihood of inducing pesticide resistance in pests, and can be applied to the biological control of the fall webworm.

This application relates to the field of pest control, specifically providing a trunk-encircling pupation device for controlling the fall webworm and its usage method. The device includes a base box, fixing rods, connectors, and a fence. The base box has an open top, and two fixing rods are fixedly installed on the inner edge of the base box, located at both ends of the inner edge. Connectors are connected to the upper ends of the two fixing rods, extending outwards from the base box. The suspended ends of the two connectors connect to both ends of the fence. The area enclosed by the fence, connectors, and fixing rods is larger than the area of ​​the open top of the base box. In use, the fixing rods are tied to the tree trunk, and rod-shaped filler is placed between the base box and the fence. After pupation is complete, the rod-shaped filler is removed and disposed of, and replaced with new rod-shaped filler. Multiple gradually narrowing gaps are formed in the rod-shaped filler, providing a stable and well-ventilated pupation environment for the fall webworm, resulting in good pupation attraction.

The invention discloses an avermectin-loaded alpha ZIF nano biopesticide preparation as well as a preparation method and application thereof, and belongs to the technical field of pesticide preparations. The abamectin-loaded alpha ZIF nano biopesticide preparation is prepared by the following method: dispersing abamectin in methanol to obtain an abamectin solution, then stirring and adding alpha ZIF nanoparticles into the abamectin solution, continuing to stir for 0.1-72 hours, centrifugally collecting precipitates, washing, purifying and drying to obtain the abamectin-loaded alpha ZIF nano biopesticide preparation. The avermectin-loaded alpha ZIF nano biopesticide preparation is obtained. The alpha ZIF nano-particles prepared by the invention can penetrate through plant tissues and net screen barriers made of pests due to the excellent nano-size, carry drugs to be transported to plant multi-tissue parts, and are used for preventing and treating defoliating pests such as fall webworms and the like; according to the avermectin-loaded alpha ZIF nano-biopesticide preparation prepared by the invention, the penetrability of avermectin to barks can be improved, and the transmission speed of avermectin in trunks can be increased.

This invention discloses an RNA biopesticide targeting the HcAmy2 gene of the fall webworm and its preparation method, relating to the fields of biopesticides and green pest control technology. The active ingredient of the biopesticide is double-stranded RNA targeting the α-amylase gene of the fall webworm, namely dsHcAmy2, and is encapsulated by chitosan to form a chitosan / dsRNA nanocomposite. The degree of deacetylation of the chitosan is ≥95%, and the viscosity is 100-200 mPa·s. The nanoparticles prepared by this invention can effectively protect dsRNA from degradation, significantly enhance its stability and RNA interference efficiency, effectively silence the key gene HcAmy2 of the fall webworm, destroy its multi-host adaptability, and have a highly efficient and specific control effect on fall webworm larvae.

The invention discloses a pest control engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of engineering bacteria and gene interference. The engineering bacteria disclosed by the invention are obtained by exogenous transfer of dsRNA fragments of pest target genes into plant probiotics. The engineering bacterium disclosed by the invention can stably express dsRNA fragments of pest target genes, can interfere the pest target genes when being used for pest control, reduces pupation rate, and has a good control effect on pests; the engineering bacterium disclosed by the invention can be colonized in various host plants of polyphagous insect fall webworms, such as poplars and mulberries, so that broad-spectrum prevention and control can be realized. Meanwhile, the engineering bacterium retains the original advantages of strong environmental adaptability, convenient genetic manipulation, high product stability and the like of plant probiotics, can exert the plant probiotic characteristics of the plant probiotics, assists the healthy growth of plants while realizing accurate prevention and control of pests, and has a good application prospect. And a promising tool is provided for pest control and synchronous and sustainable protection of various host plants.

The invention discloses an artificial screening and breeding method for hyphantria cunea egg-stage parasitic wasps and application of the artificial screening and breeding method. According to the method, the parasitic wasps in the egg period for preventing and treating fall webworms are screened out, and the parasitic ability of the parasitic wasps to fall webworm eggs is enhanced through artificial domestication; key factors such as alternative hosts, the most suitable host day age and the most suitable temperature for artificially breeding the parasitic wasps are determined, and the parasitism rate and the offspring number of the parasitic wasps are effectively increased. The parasitic wasps bred by the method are applied to field control of hyphantria cunea eggs, and when the parasitic wasps are directly released, the best control effect can be achieved according to the benefit-harm ratio of 1: (8-12).

The invention relates to the technical field of prevention and control of agricultural and forestry pests, and discloses a synergistic protein ORF42 of insect baculovirus and application of the synergistic protein ORF42. According to the invention, a synergistic protein (ORF42) is obtained from an insect biocontrol resource, i.e., a Pinus fumosa nuclear polyhedrosis virus, and protein function verification shows that the protein has a remarkable synergistic effect on a fall webworm nuclear polyhedrosis virus, an apocheima cinerarius nuclear polyhedrosis virus and a Pinus fumosa nuclear polyhedrosis virus; the compound can be used as a synergistic factor to be added into insect viruses, and has important significance on prevention and control of major forestry pests (fall webworms, spring inchworm and smoke wing pine bees).