Beauveria bassiana strain having high virulence to fall webworm and application thereof

A technology of Beauveria bassiana, American white moth, applied in the direction of application, fungi, microbial-based methods, etc.

Inactive Publication Date: 2013-09-18
텐진인스티튜트오브플랜트프로텍션
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of Beauveria bassiana to control white moth has also been reported in Shandong, but there is currently no Beauveria bassiana preparation registered as a pesticide in my country.

Method used

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  • Beauveria bassiana strain having high virulence to fall webworm and application thereof
  • Beauveria bassiana strain having high virulence to fall webworm and application thereof
  • Beauveria bassiana strain having high virulence to fall webworm and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1 Collection, isolation and purification of Beauveria bassiana Bb10331 and other 10 strains of Beauveria bassiana:

[0032] Beauveria bassiana was screened from soil by trapping Tenebrio molitor (commercially available) and Beauveria bassiana selective medium separation method; by dilution separation method, it was isolated and purified from the pupae of Beauveria bassiana infected by Beauveria bassiana.

[0033] Obtain Beauveria bassiana preparations and strains from manufacturers and scientific research institutes of Beauveria bassiana preparations.

[0034] The method of purifying the Beauveria bassiana strain from the preparation adopts the dilution separation method. The results are shown in Table 1:

[0035] Tenebrio trapping method: First, put the soil sample through a fine sieve and add an appropriate amount of water to make the water content reach a wet state (hold it into a ball, touch it lightly), and fill two-thirds of it into a 90mm diameter petri dish. M...

Embodiment 2

[0043] Bb10331 Beauveria bassiana strain sporulation structure and conidia morphology observation, strain identification:

[0044] Method: The structure of sporulation cells and the morphology of conidia were directly observed under a microscope by glass slide culture method; the morphology of sporulation cells was observed. Microscopic rulers measure the length and width of conidia.

[0045] Observation results: The colony morphology, sporulation structure and conidia morphology of the Bb10331 strain are shown in Table 2 and Table 3, respectively:

[0046] Strain code 10d colony morphology on SADY medium Bb10331 White, relatively flat, the central 2 / 3 area is milky white spore powder layer with more obvious radial ridges; the outside is white felt-like, radial ridges are not obvious or absent. The initial stage of the colony is papillary

[0047] Strain code Sporulation structure and characteristics of conidia Bb10331 Most of the co...

Embodiment 3

[0050] Molecular identification of Bb10331 Beauveria bassiana strain (the reagents used are commercially available):

[0051] Method: Take 100 mg of Beauveria bassiana spores and mycelia cultured on Sabouraud medium, put them in a clean and sterilized mortar, seal them with plastic wrap, freeze them together with the mortar sticks in a -20°C refrigerator, and put them Mortar on ice for 5-8min, add 3ml of FG1 Buffer in the fungal extraction kit in 2 times, mix well, transfer to a 15ml centrifuge tube and add 20ul 20mg / ml RNase A (RNA nuclease A, commercially available); the centrifuge tube In the water bath at 65°C for 60 minutes, fully mixed twice during the period; after the water bath, add 700ul FG2 Buffer (buffer solution) and mix well, then ice-bath for 10 minutes, and centrifuge at 8000g for 10 minutes in a refrigerated centrifuge; take the supernatant and transfer it to another 15ml tube Add 1.5 times FG3 (a component in the fungal DNA extraction kit, commercially availa...

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Abstract

The invention discloses a beauveria bassiana strain having high virulence to fall webworm and a preparation method of the beauveria bassiana strain. The preparation method comprises the following steps of: acquiring high-toxicity strain of beauveria bassiana, identifying the strain, and screening. The Bb10331 strain screened by the invention, which has the preservation number of CGMCCNo.5739, is the strain obtained by purifying the fall webworm over-wintering pupae body infected with the beauveria bassiana acquired by the inventor from Huodun of Wuqing district in Tianjing. Due to the morphologic observation of the bacterial colony, the spore production structure and the conidium and the detection of DNA-ITS sequence, the train is identified to be beauveria bassiana. The laboratory toxicity test, the control test to the fall webworm happened on poplars among the fields and the aphid test on the cucumber crop in control protective land show that the strain is good in development potential and production application value.

Description

technical field [0001] The invention belongs to the technical field of microbial insecticides, and relates to a strain of Beauveria bassiana with high pathogenicity to American white moth and a preparation method thereof, more specifically, an internationally important quarantine pest-American white moth screened out The process of collection, identification and screening of a Beauveria bassiana strain with high pathogenicity. Background technique [0002] For a long time, Beauveria bassiana has been widely concerned and studied as an important factor in the biological control of pests. my country began the research and application of Beauveria bassiana in the 1950s. The use of Beauveria bassiana to control pine caterpillars and corn borers is one of the largest and most successful cases of insecticidal fungi in the world. The application area is 500,000 hectares, and the application area exceeds 1.33 million hectares in the highest year. According to incomplete statistics,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 刘宝生谷希树许静杨胡霞孙淑琴白义川徐维红
Owner 텐진인스티튜트오브플랜트프로텍션
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