48 results about "Beauveria sp." patented technology

The invention mainly provides Beauveria bassiana ZABb200102 and application of the Beauveria bassiana ZABb200102, the Beauveria bassiana ZABb200102 is preserved in China General Microbiological Culture Collection Center on October 28, 2024, and the preservation number of the Beauveria bassiana ZABb200102 is CGMCC (China General Microbiological Culture Collection Center) No. 41557. The strain is separated from sick lepidoptera cacalidae larva bodies for the first time, and it is found through toxicity determination that the strain has high pathogenicity to tomato leaf miner larvae. In addition, the strain can also be colonized in tomatoes, so that the growth of the tomatoes is promoted, and the resistance of the tomatoes to pests is indirectly improved. Therefore, the strain can be widely applied to development of tomato bacterial fertilizer and green prevention and control of tomato leaf miner which is an important tomato pest.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The present application belongs to the technical field of preparation of white-backed caterpillar. More particularly, it relates to a Beauveria bassiana agent for greatly improving the time concentration of death of domestic silkworm and a high-quality white-backed caterpillar production method. The present application provides a Beauveria bassiana agent containing a strain of Beauveria bassiana and a fermentation product of another strain of Beauveria bassiana without bacteria bodies. The use of the agent in the present application to infect domestic silkworm can shorten the time of complete death of domestic silkworm, and concentrate the death time of domestic silkworm at the 6th day after inoculation, with a time span of only 24 hours, which helps to improve the production efficiency of white-backed caterpillar, effectively reduces the labor of feeding and picking silkworms during the death stage, and reduces the production cost. In addition, the method for producing white-backed caterpillar in the present application can achieve good infection effect by spraying the agent once, reducing the process of white-backed caterpillar production. And the white-backed caterpillar produced by the method in the present application is uniform in size, the consistency of product quality parameters is good, and the quality is stable, which helps to improve the clinical application effect.

The invention belongs to the technical field of biological control, and particularly relates to beauveria bassiana TYW001 and application thereof. The invention provides beauveria bassiana TYW001, the Latin name of the beauveria bassiana TYW001 is Beauveria bassiana, the beauveria bassiana TYW001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 30, 2025, and the preservation number is CGMCC NO.42149. The strain not only can resist high temperature and drought, but also can resist high temperature and drought, but also can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and can resist high temperature the soybean megalurothrips can be effectively prevented and treated; moreover, the beauveria bassiana TYW001 can enhance the predation effect of the orius minutus on the megalurothrips megalurothrips, and a reliable green prevention and control technology is provided for prevention and control of the megalurothrips megalurothrips.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The invention relates to the technical field of prevention and control of bactrocera cucurbitae, in particular to high-temperature-resistant beauveria bassiana WZS-5 and application thereof to prevention and control of bactrocera cucurbitae, and the preservation number of the high-temperature-resistant beauveria bassiana WZS-5 is GDMCCNO: 66358. The optimal growth temperature of the strain is 28-30 DEG C, and the strain still keeps certain growth and reproduction capability at the high temperature of 35 DEG C. Biological assay results show that under the conditions that the temperature is 30 DEG C and the relative humidity is 70%, the prevention and treatment effect of the strain on beauveria bassiana is remarkably better than that of common beauveria bassiana, and the accumulative death rate of bactrocera cucurbitae adults under the concentration of 1 * 10 < 9 > spores / mL reaches 89%. Results show that the strain has excellent high-temperature adaptability and strong pathogenic activity, can efficiently control bactrocera cucurbitae populations in tropical and subtropical high-temperature environments, and provides new microbial resources and technical approaches for green prevention and control of fruit and vegetable pests.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.

The invention relates to the technical field of microorganisms, in particular to beauveria bassiana QZ and application thereof. The collection number of the beauveria bassiana QZ is GDMCC No: 66669. The beauveria bassiana QZ provided by the invention has strong pathogenicity to coffee berry bark beetles, the strain is modified to express and deliver double-stranded RNA-dsDorsal, the pathogenicity is stronger, laboratory data shows that the fatality rate is almost 100% within 8 days, and the lethal ability is improved by 20% compared with that of a non-transgenic strain.

Disclosed herein are compositions comprising A) compound of Formula One (F1) or Formula Two (F2); and B) biological agent selected from the group consisting of Bacillus species, Baclovirus species, Pseudomonas species, Beauveria species, Mycobacterium species, Mycorrhizal fungi, Trichoderma species, and Metarhizium species.

The invention discloses two odontotermes formosanus OfCTLs genes and application of dsRNA of the odontotermes formosanus OfCTLs genes. Wherein the two odontotermes formosanus OfCTLs genes are an OfCTL-S3 gene and an OfCTL-S15 gene respectively, and the corresponding nucleotide sequences of the odontotermes formosanus OfCTL-S3 gene and the OfCTL-S15 gene are SEQ ID NO: 1 and SEQ ID NO: 2 respectively. Recombinant OfCTL-S3 and OfCTL-S15 proteins are obtained through a prokaryotic expression system, and an in-vitro antibacterial test proves that the OfCTL-S3 and OfCTL-S15 proteins have antibacterial activity and agglutination effects on beauveria bassiana, gram-positive bacterium bacillus thuringiensis and gram-negative bacterium serratia marcescens in the presence of calcium ions. According to the invention, an odontotermes formosanus OfCTLs RNA interference system carried out by using a feeding method is established, and an obvious gene silencing effect is obtained by feeding odontotermes formosanus. The dsRNA of the odontotermes formosanus OfCTLs is combined with the biocontrol bacteria, so that the control effect of the biocontrol bacteria can be remarkably improved.

The application discloses a Beauveria bassiana strain SL-066 and application thereof, and relates to the technical field of biological strains. The strain provided by the application is preserved in the China General Microbiological Culture Collection Center on August 8, 2022, and the preservation number is CGMCC No. 40269. The Beauveria bassiana strain SL-066 provided by the application can effectively prevent and control various rice pests, is a Beauveria bassiana strain capable of preventing and controlling the coleopteran pest Curculionidae (rice water weevil adult), the coleopteran pest Chrysomelidae (rice water weevil larva) and the lepidopteran pest Chilo suppressalis (Chilo suppressalis larva), and has a good prevention and control effect on the three common pests on rice.

The application provides a Beauveria bassiana strain Bb-YY for preventing and controlling various diseases and pests, a derivative product and application thereof, and belongs to the technical field of functional microorganisms. Beauveria bassiana The strain Bb-YY is screened from the rhizosphere soil of a peanut healthy plant planted in a serious continuous cropping obstacle plot, and is identified as Beauveria bassiana (Bb) by molecular identification, has high efficient prevention and control effects on white grubs, peanut fruit rot pathogens and peanut root rot pathogens, and has certain inhibition effects on Bacterospora coriacea causing tobacco black foot disease and Pyricularia oryzae causing rice blast. It can be seen that the Bb-YY strain can also play a prevention and control effect on tobacco, rice and other crops in addition to peanut planting, and can effectively reduce the use of chemical pesticides, and provides a strong support for green prevention and control of peanut diseases and pests.

The invention relates to the technical field of agricultural microorganism application, in particular to a beauveria bassiana Bb455 strain with efficient nitrogen fixation capacity and application of the strain in promotion of corn growth, and the beauveria bassiana Bb455 strain is suitable for green planting of grain crops such as corn and the like, can reduce application of chemical nitrogen fertilizer and can promote sustainable development of agricultural ecology.

The invention belongs to the technical field of biocontrol microorganism genetic engineering, and particularly relates to an sfgA gene for regulating and controlling growth and development of beauveria bassiana and application of the sfgA gene. The invention discloses an sfgA gene for regulating and controlling growth and development of beauveria bassiana, the nucleotide sequence of the sfgA gene is as shown in SEQ ID NO.1, and the amino acid sequence of encoded protein of the sfgA gene is as shown in SEQ ID NO.2. By systematically researching the effect of the development signal protein sfgA in the asexual spore production process of the beauveria bassiana and the regulation and control mechanism of the development signal protein sfgA, the growth and development of the beauveria bassiana can be regulated and controlled. A theoretical basis is laid for researching a filamentous fungus asexual sporulation molecular mechanism, and a new scientific basis and gene resources are provided for mining and genetic improvement of biocontrol potentials of biocontrol fungi.

This invention relates to a triple nano-PCR detection method for silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus, belonging to the field of molecular diagnostic technology. Addressing the technical problem of existing silkworm disease diagnostic methods' inability to simultaneously, efficiently, and sensitively identify these three commonly mixed infection pathogens, this invention provides an integrated solution. The key technical points include extracting DNA from the sample to be tested, using this DNA as a template, performing nano-PCR amplification in the same reaction system using a specific primer set, and subsequently detecting the amplification products by agarose gel electrophoresis. The infection status of silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus is determined based on the specific bands at 733 bp, 230 bp, and 860 bp, respectively. This method is mainly used for the rapid, simultaneous diagnosis and identification of mixed infections of microsporidia, Beauveria bassiana, and hemorrhagic septicemia during silkworm rearing.

The present invention provides stable, non-aqueous biopesticides. The present invention relates to biopestic formulations of entomopathogenic fungi or their spores. In particular, the present invention discloses oil dispersions containing entomopathogenic fungi or their spores, especially Beauveria bassiana. The present invention also relates to methods for preparing the formulations and their use.

The present application relates to the field of microorganisms, and particularly relates to a preparation method and application of a Beauveria bassiana BC-01 mycelium polysaccharide, wherein the Beauveria bassiana BC-01 has been preserved in the China General Microbiological Culture Collection Center on January 18, 2024, and the preservation number is CGMCC No. 41044. The strain produces mycelium polysaccharide with an IC 50 value of 0.5302 mg / mL for inhibiting tyrosinase; and a polysaccharide concentration of 1.5 mg / mL shows good whitening effect. The polysaccharide promotes the expression of cytokines IL-6 and TNF-alpha, and shows immune activity. The polysaccharide has the effects of whitening, antioxidation, and immune activation, and can promote the proliferation of Staphylococcus epidermidis and inhibit the proliferation of Propionibacterium acnes, and can be used as a new whitening and skin microecological material for developing cosmetics. The preparation method of the Beauveria bassiana mycelium polysaccharide is simple and easy to operate, the extraction process does not use organic reagents such as ethanol and methanol, and the production is safe and low in price.

The invention discloses a high-virulence beauveria bassiana Zeb-01 strain and application of the high-virulence beauveria bassiana Zeb-01 strain to control of palm pests, the high-virulence beauveria bassiana Zeb-01 strain is beauveria bassiana, and the preservation name of the high-virulence beauveria bassiana Zeb-01 strain is Beauveria bassiana; the strain is preserved in the China General Microbiological Culture Collection Center, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is November 15, 2025; the preservation number of the strain is CGMCC (China General Microbiological Culture Collection The separated beauveria bassiana Zeb-01 strain has extremely high pathogenicity to rhynchophorus ferrugineus and rhinoceros rhinoceros, the lethal effect of the strain is remarkably higher than that of commercially available beauveria bassiana preparations, the strain has more outstanding advantages under the low-concentration condition, the lethal time of the strain to the two pests is remarkably shorter than that of commercially available strains, the pest hazard period is effectively shortened, and the environment pollution is reduced. And the insecticidal composition has the characteristics of high toxicity, high insecticidal rate and wide range of applicable pests.

The present application relates to the field of steroid drugs and biotechnology, and relates to a novel steroid hydroxylase CYP68BE1 from Beauveria bassiana, a coding gene thereof and application. Specifically, the present application provides a novel steroid hydroxylase CYP68BE1 from Beauveria bassiana, a nucleic acid molecule coding the enzyme, a vector containing the nucleic acid molecule, and a recombinant bacterium containing the vector or having the nucleic acid molecule integrated therein. Through recombination of yeast bacteria or microsomal transformation of steroids, it is found that the CYP68BE1 enzyme can catalyze the hydroxylation reaction of C11α and / or C6β and / or C1β and / or C1α of steroid compounds. Among them, the transformation products include various steroid drug intermediates and / or steroid active compounds, and the present application has great application potential and economic value.

The invention discloses beauveria bassiana GIZ-0fBb1 for preventing and treating two types of invading ants and application of the beauveria bassiana GIZ-0fBb1. The beauveria bassiana is named as Beauveria bassiana GIZ-0fBb1, and the preservation number of the beauveria bassiana GIZ-0fBb1 is GDMCC No: 67403. The GIZ-0fBb1 is high in growth speed, high in sporulation speed, large in sporulation quantity and good in insecticidal effect, is a biocontrol strain with a good application prospect in sustainable prevention and control of the solenopsis invicta and the small imported fire ants, and can be widely applied to biological prevention and control of the solenopsis invicta and the small imported fire ants.

The application provides a method for preparing Beauveria bassiana protoplast by using lyticase. The method comprises the following steps: obtaining Beauveria bassiana mycelium and washing the mycelium with a NaCl solution; mixing the washed mycelium with an enzyme solution according to a ratio of 1g:50mL and oscillating the enzyme solution for enzymolysis; after the enzymolysis is completed, post-treating the enzyme solution to obtain the Beauveria bassiana protoplast; and the concentration of the lyticase in the enzyme solution is 5-20g / L per gram. Under the optimal enzymolysis condition, the yield of the Beauveria bassiana protoplast reaches 2.56*10 7 6 / mL, which is about 2.56 times higher than that of the prior art.

The invention relates to application of silkworm excrement as a raw material of a culture medium for producing beauveria bassiana, and a method for producing the beauveria bassiana by utilizing silkworm excrement waste, belonging to the field of fermentation. The invention also provides a beauveria bassiana solid culture medium which is characterized in that the raw materials of the culture medium contain silkworm excrement. The invention provides a fermentation culture technology using waste silkworm excrement, and aims to improve the production efficiency of beauveria bassiana and provide a new resource utilization mode for the production of bombyx batryticatus. The technology not only can effectively reduce the environmental pollution caused by wastes, but also can improve the production efficiency, save the raw material cost and improve the product quality.

This invention relates to the field of spore collection technology, specifically to a collection device for Beauveria bassiana spores from the medicinal silkworm. It includes a frame, a horizontal vibrating collection frame slidably connected to the frame, and a horizontal drive mechanism on the frame that drives the horizontal vibrating collection frame to vibrate alternately with a first, second, and third amplitude. A vertical vibrating collection frame is slidably connected to the horizontal vibrating collection frame, and a vertical drive mechanism between the two drives the vertical vibrating collection frame to vibrate alternately with a fourth, fifth, and sixth amplitude. A front collection cylinder and a rear collection cylinder are both fixedly connected to the horizontal vibrating collection frame. A collection shaft tube coaxially arranged with the front collection cylinder and a conveying shaft coaxially arranged with the rear collection cylinder are rotatably connected to the vertical vibrating collection frame. The beneficial effect of this invention is that it organically integrates mechanical peeling, airflow purging, and negative pressure suction to form an integrated continuous collection process of peeling, purging, and suction.

This utility model relates to the field of microbial fermentation engineering technology, specifically to a solid-state fermentation device for Beauveria bassiana, including a fermentation chamber. A fan and a ventilation pipe are respectively installed at the upper and lower ends of the fermentation chamber. A rotating sleeve is rotatably connected inside the fermentation chamber. A motor is fixedly connected to the bottom of the fermentation chamber, and the output end of the motor is fixedly connected to the bottom end of the rotating sleeve. A cooling and oscillation mechanism periodically lifts the culture tray through a cam to generate oscillation, which, together with the air circulation of the fan and ventilation pipe, enhances the heat exchange between the substrate and the cold air. The spiral stirring blade has a double-layer hollow structure and is connected to the drainage hole to guide the discharge of condensate or cleaning wastewater. At the same time, the hollow structure reduces the temperature of the stirring blade itself and assists in the heat dissipation of the substrate.

The application discloses a kind of real fly luring and killing agents.The real fly luring and killing agent is composed of the following components by mass percentage: 75-85% of apple snail meat hydrolysate, 3-7% of pest toxic components, 5-15% of auxiliary materials and 2-5% of stabilizers.The apple snail meat hydrolysate is a mixture of apple snail meat after protease hydrolysis and enzyme inactivation, and the pest toxic components are Metarhizium or Beauveria.The luring and killing agent uses the protein hydrolysate of invasive species apple snail meat as the main raw material to prepare food lure, and then adds a small amount of Metarhizium / Beauveria to prepare the luring and killing agent.The synergistic effect of food lure and Metarhizium / Beauveria significantly improves the luring and killing effect on real flies.The new real fly luring and killing agent has the characteristics of high efficiency and green environmental protection.

The utility model discloses a beauveria bassiana solid state fermentation device which comprises a constant temperature box body, a supporting mechanism and a washing mechanism, a guide rail is arranged on the inner wall of the constant temperature box body, the supporting mechanism comprises a vertical rod, a top rod arranged on the side wall of the vertical rod and a plate frame movably matched with the vertical rod, and the washing mechanism is arranged on the vertical rod. The flushing mechanism comprises an electric telescopic rod, a telescopic sleeve and a high-pressure spraying pipeline, the telescopic sleeve is movably matched with the telescopic end of the electric telescopic rod, the high-pressure spraying pipeline is arranged on the side wall of the telescopic sleeve, an inlet of the high-pressure spraying pipeline is communicated with a movable connector, and the movable connector is arranged on the side wall of the constant-temperature box body. The tray frame can be put down and hung on the guide rail through the ejector rod, dirt on the surface can flow down along water flow conveniently during washing, cleaning is more thorough, the electric telescopic rod is matched with the telescopic sleeve, the high-pressure spraying pipeline is pushed to turn over, and comprehensive cleaning work on the interior of the constant-temperature box is achieved.

The invention discloses a beauveria bassiana strain with high yield of beauveria bassiana, which is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M2022052, and the preservation time is January 17, 2022. The invention further discloses a microbial agent, a preparation method thereof and application of the microbial agent in preparation of stiff silkworms. The beauveria bassiana can effectively infect silkworm bodies, bombyx batryticatus with the high beauveria bassiana content is obtained, compared with common strains, the beauveria bassiana can more quickly infect and overgrow the surfaces of the silkworm bodies, the content of the beauveria bassiana in the obtained bombyx batryticatus medicinal materials is higher, and the beauveria bassiana has the remarkable advantages of inhibiting bacterial growth, improving the yield of the bombyx batryticatus and improving the content of medicinal components and is good in application prospect.

The invention belongs to the technical field of microbiology, and particularly relates to a beauveria bassiana strain JC-01 and application of the beauveria bassiana strain JC-01 to prevention and treatment of heortia vitessoides. The invention relates to a beauveria bassiana strain JC-01, which is preserved in Guangdong Microbial Culture Collection Center on March 11, 2025, the preservation number is GDMCC No: 65998, and the taxonomic name is Beauveria bassiana. The beauveria bassiana JC-01 disclosed by the invention has relatively high pathogenicity to the heortia vitessoides, can effectively kill the heortia vitessoides, and has a relatively high bombyx batryticatus rate. The beauveria bassiana JC-01 disclosed by the invention is a biological prevention and control strain with potential significance in prevention and control of the heortia vitessoides.

The invention relates to application of a bombyx mori BmSPP gene in preparation of silkworm larva, and belongs to the field of biological genetic engineering. The method aims at solving the problems that traditional silkworm larva production depends on natural infection, the source of silkworm larva is unstable, and the yield is low. Existing silkworm variety breeding is not sensitive to beauveria bassiana, so that the artificial breeding efficiency is low. According to the invention, a BmSPP gene of bombyx mori is knocked out through a CRISPR / Cas9 gene editing technology, and a bombyx mori strain BmSPPKO with high sensitivity to beauveria bassiana is created. The method comprises the following steps: designing an sgRNA vector targeting BmSPP, microinjecting the sgRNA vector into silkworm eggs, carrying out fluorescence screening and PCR verification to obtain a heterozygous mutant, and inoculating the heterozygous mutant with beauveria bassiana to produce bombyx batryticatus. According to the method, the infection efficiency is remarkably improved, the production period is shortened, the production cost is reduced, the limitation of traditional breeding is broken through, special silkworm eggs are provided for large-scale and standardized production of silkworm larva, and the industrial pain point of unstable sources is solved.