195 results about "Beauveria sp." patented technology

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil/land and especially can be used for effectively improving soil polluted by heavy metals.

A wettable powder of bacillus thuringiensis and beauveria bassiana relates to a mixed biopesticide, aiming to the shortcomings of prevention and treatment limitations of beauveria bassiana and bacillus thuringiensis (Bt), particularly poor quick-acting property of microbial pesticides. The wettable powder provided in the invention is prepared from the following components: 10-14wt.% of bacillus thuringiensis, 4-14wt.% of beauveria bassiana, 3-5wt..% of wetting agent, 10-20wt.% of dispersant, 0.1-0.5wt.% of ultraviolet protectant and 50-60 wt.% of carrier. The wettable powder of bacillus thuringiensis and beauveria bassiana provided in the invention is high in activity, stable in effect, long in persistence and convenient for use.

The invention relates to a novel microbial organic fertilizer with anti-actinomyces active compound bacteria and a preparation method thereof. The novel microbial organic fertilizer comprises a microbial inoculum and an organic fertilizer, wherein the microbial inoculum comprises colonies of Bacillus siliceous, Nocardia actinomyces, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus laterosporus, azotobacter, Beauveria bassiana, Metarrhizium anisopliae, Trichoderma and Saccharomyces, and peptide proteins and rare-earth elements having unique effects on inhibiting wireworms and enhancing plant root system stress tolerancen; and the organic fertilizer comprises animal manure and plant fibers. The novel microbial organic fertilizer is prepared by fermenting the animal manure and plant fibers by using the microbial inoculum. The microbial organic fertilizer can quickly and effectively kill wireworm ova in soil, has strong effects on inhibiting and killing larvae and imagos, can continuously improve the growth environment of the plant root by using the growth of the microbial colonies and the stress tolerance of the peptide proteins, enables the plant to have strong roots, thick stems and green leaves, postpones the aging of the plant, and promotes prematurity and yield increase.

The present invention provides a strain of entomopathogenic Beauveria bassiana, compositions comprising the entomopathogenic fungi strain or metabolites of the strain, and the use of the entomopathogenic fungi strain and compositions as biological control agents. Methods for the biological control of phytopathogenic insects using an entomopathogenic Beauveria bassiana fungi strain or one or more metabolites thereof, optionally together with other entomopathogenic fungi including fungi selected from strains of Lecanicillium spp., Paecilomyces fumosoroseus, and compositions comprising said fungi or metabolites thereof are also provided.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, can survive for a long term in a plurality of improved soil to play a role, is good in adaptation of improving soil/land and especially can be used for effectively improving a plurality of soil, such as secondary salinization land, desertification soil.

The invention relates to the technical field of agricultural planting, in particular to a composite microbial agent for citrus tristeza virus, a preparation method thereof and a special biological agent for the citrus tristeza virus prepared by using the same. According to the invention, bacillus thuringiensis, beauveria bassiana and trichoderma harzianum are used to control the pathogen of citrustristeza virus pathogens on the ground and kill a spreading source wood louses and eggs thereof so as to effectively control the occurrence of the citrus tristeza virus, and four kinds of microorganism bacteria underground can effectively control the invasion of pathogenic microorganisms, effectively control the spread of pathogenic bacteria by nematodes, and simultaneously regulate the soil structure, decompose organic matters and improve the soil fertility. By promoting the growth of citrus roots with citrus specific AM fungi, citrus can grow new roots in clusters, the number of the new roots is enlarged, the tree vigor is enhanced, the immunity and disease resistance of trees are improved, a nitrogen fixation system is formed, and the pathogen control is combined with the improvement of the ecological environment for the growth of the citrus to achieve the effect of preventing and treating the citrus tristeza virus.

An agricultural chemical for preventing and killing pine moth is prepared from Beauveria bassiana (CGMCC No.1573) through slant culture, culturing in jar, culturing in bag, and mixing with nutritive wet material. It has high effect (70-80%) and no pollution.

The invention discloses a Beauveria bassiana BbL25 strain for preventing and treating solenopsis invicta. The strain is preserved at China Center for Type Culture Collection on February 16, 2017 and the preservation number is CCTCC NO: M2017045. The Beauveria bassiana BbL25 strain is highly pathogenic to the solenopsis invicta, and half lethal concentration (LC50) to the solenopsis invicta is 8.15x10<5> conidium/mL on the 14th day after inoculation, and the strain has a good field controlling effect on the solenopsis invicta; the controlling effect of 1x10<7>/mL condinal suspension on the solenopsis invicta reaches 82.52% on the 14th day after drug delivering, and the effect is same as chemical insecticide 0.25% fluoxane. The Beauveria bassiana BbL25 strain can be used as a living biological insecticide and has no pollution to the environment and is non-toxic to human beings and animals and is suitable for the production of organic agricultural products, and great application prospects are achieved.

The invention relates to a preparation method of Beauveria sp. extract with functiosn of a tyrosinase inhibitor and an antioxidant. The preparation method comprises the following operation steps: 1. selecting and culturing Beauveria sp. Strain; and 2. extracting and refining active components from a fermented product, wherein the refining process comprises primary refining and chromatographic refining. The Beauveria sp. and Beauveria sp. extract provided by the invention have tyrosinase inhibiting and antioxidation active effects, and the refined product has the minimum IC50 (50% inhibiting concentration) of 134.5 mu g/ml to tyrosinase and the minimum IC50 of 87.2 mu g/ml to rat liver cell lipid oxidation; the Beauveria sp. extract can be used as articles for daily use to take into effects of whitening, antioxidation, antiaging, preserving, sterilizing, anti-inflammatory and the like; because the Beauveria sp. extract is produced by microorganism fermentation, the production of the Beauveria sp. extract is not influenced by environment and natural resources, and is easy to industrialize and automatize; the process method has low production cost, simple process, stable process, and high success rate and is easy to adjust and control; and the preparation method is suitable for investment of enterprises in various scales.

The invention discloses a recombinant beauveria brongniartii proteinase K mutant PK-M1 and a preparation method of the recombinant beauveria brongniartii proteinase K mutant PK-M1. According to the technical scheme, starting from transforming the molecular structure of proteinase K of recombinant beauveria brongniartii, the proteinase K mutant PK-M1 with the activity and stability both improved isobtained, and the amino acid sequence is as shown in SEQ ID NO:1; an expression system is optimized, by utilizing the yeast cell external secretion expression technology, the recombinant proteinase Kmutant can be fermented in large scale and efficiently expressed, and further, the yield of proteinase K is improved; and with the protein purification manner realizing efficient affinity chromatography, the yield of the proteinase K in the purifying process is improved, and thus the large-scale production of the proteinase K is realized.

The invention discloses a protease inhibitor BmSPI38, which comprises the 23rd and 80th amino acids of SEQIDNo. 2. The temperature and the pH of the BmSPI38 are very stable, the activity of subtilisin, proteinase K, degrading protease CDEP-1 on the wall of beauveria bassiana and semialkaline paotease can be inhibited strongly, the CDEP-1-induced excessive or harmful blackening of silkworms can be blocked, and the protease inhibitor can be applied in preparation of subtilisin inhibitors, blockers against CDEP-1-induced insecticidal blackening reaction, and can also be used for culturing silkworms with pathogenic fungi resistance and improving the resistance of the silkworms against invasion of pathogenic fungi. In addition, the operation of the BmSPI38 prokaryotic expression is simple, and the expression quantity is large.

The invention relates to a preparation method of an organic fertilizer special for burdock. The method includes: preparing a beauveria bassiana liquid seed; preparing an organic fertilizer raw material 1; adding an EM strain into brown sugar water according to a mass ratio of 1:80-100, fully stirring the substances evenly, then spraying the mixture into a solid matrix; preparing an organic fertilizer raw material 2; mixing the organic fertilizer raw material 1 prepared in step 2 and the organic fertilizer raw material 2 prepared in step 4 according to a mass ratio of 1:30-50, performing granulation, then conducting hot air drying at a temperature of less than 50DEG C, and carrying out packaging so as to obtain the beauveria bassiana hypha and spore powder-rich organic fertilizer special for burdock. The organic fertilizer provided by the invention contains a lot of organic matters, and simultaneously contains beneficial microbial floras having strong environmental adaptability, can easily give play to the population advantages, has strong nutritional functions, and can achieve good rhizosphere growth promoting effect. Integrating the advantages of organic fertilizers' ''stability'' and bacterial fertilizers' ''promotion'', the organic fertilizer provided by the invention can meet the nutritional needs of burdock at different growth stages, and can enhance the fertilizer's nutrient utilization rate from about 30% to 40%-50%.

The invention discloses a new beauveria bassiana strain XWY-1 which has high poisoning capability on forestry important insects and especially on coleoptera chrysomelidae insect pyrrhalta aenescens. Toxicity tests show that XWY-1 strain has extremely toxicity on coleoptera chrysomelidae insect pyrrhalta aenescens, and therefore beauveria bassiana XWY-1 can be prepared into pesticides for controlling forestry insects, and thus the use amount of pesticides is reduced, the environmental pollution is reduced, natural enemies are protected, and beauveria bassiana XWY-1 has important economical value and application prospect.

The invention relates to the field of agricultural microorganisms, and discloses a beauveria bassiana and an application thereof. The preservation number of the beauveria bassiana disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 21047. In addition, the invention also provides an application of the beauveria bassiana in preparation of a hydroxamic acid type siderophore and regulation of plant diseases. The beauveria bassiana disclosed by the invention is a dominant strain of high-yield siderophore, and can be used for synthesizing and secreting the hydroxamic acid type siderophore. The secondary metabolites of the beauveria bassiana disclosed by the invention have antibacterial activity on pseudomonas solanacearum, pectobacterium carotovorumand ralstonia solanacearum.

The invention discloses a method for producing a heavy metal absorbent through microbiological treatment on lignite and a related compound microbial agent, relating to a method for preparing the heavy metal absorbent through the microbiological treatment. The compound microbial agent is composed of fermentation liquor of beauveria bassiana, bacillus subtilis and candida tropicalis. The compound microbial agent can react with various groups in the lignite, so that the capacity of the lignite for adsorbing heavy metal ions is further promoted, and the synergy of the three microbial strains is superior to the effect of any one microbial strain and the effect of any two microbial strains.

The invention belongs to the technical field of microorganisms, and particularly relates to beauveria bassiana USCM6, a fungicide, application of the beauveria bassiana USCM6 and a pest control method. The invention provides Beauveria bassiana Bb0901, and the collection number of the Beauveria bassiana Bb0901 is CGMCC (China General Microbiological Culture Collection Center) No.23232. The Beauveria bassiana Bb0901 can be used for preparing a strain of Beauveria bassiana. The strain provided by the invention can effectively prevent and treat spodoptera frugiperda, and has the advantages of environmental protection and difficult generation of resistance.

The invention discloses an insect-resistant medicament capable of controlling amaralin biological sources of corn borers, which comprises the following components in percent by weight: 5-9% of amaralin, 2-5% of matrine, 4-8% of citrus oil, 35-45% of beauveria bassiana spore solution and 40-50% of bacillus cereus spore solution. The insect-resistant medicament preferably comprises the following best components in percent by weight: 7% of amaralin, 3% of matrine, 5% of citrus oil, 40% of beauveria bassiana spore solution and 45% of bacillus cereus spore solution. Through actual field planting, effects of effectively inhibiting corn borer eggs and larvae as well as expelling imagoes can be achieved, and crops can be induced to generate protectin, thereby promoting the robust growth of the crops and improving the quality of the crops.

The invention discloses a microbial preparation for preventing and treating plant diseases, which is characterized by comprising the following microbial preparations in parts by weight: 10-20 parts of bacillus velezensis HAB-2, 5-10 parts of trichoderma harzianum, 1-5 parts of beauveria bassiana and 1-3 parts of metarhizium anisopliae. Wherein the preservation number of the bacillus velezensis HAB-2 is CCTCC (China Center For Type Culture Collection) NO: M 2015070. Correspondingly, the invention further provides a preparation method and application of the microbial preparation. The microbial preparation disclosed by the invention has remarkable prevention and treatment effects on areca-nut anthracnose, guava leaf blight, navel orange anthracnose, longan leaf spot disease, wax apple fruit rot and the like, and can effectively reduce the morbidity.

The invention provides a method for improving the beauveria bassiana chitinase gene disease resistance and culturing disease resistance plants adopting the method. The method is characterized in that a chitin binding structure domain of silkworm chitinase genes is fused with beauveria bassiana chitinase genes, and the disease resistance of the beauveria bassiana chitinase genes is improved. A 35S promoter is used for controlling fusion genes SwBbchit1, in addition, plant expression vectors are built and are respectively transferred into cotton and tomatoes, and the disease resistance of the transgenic cotton and tomatoes can be further improved. Compared with the disease resistance of the Bbchit1 transgenic cotton and tomatoes, the disease resistance of the SwBbchit1 cotton and tomatoes obtained by the method provided by the invention is obviously improved.

The invention discloses a strain of beauveria bassiana bh001 for preventing and treating curculio arakawai pests and application thereof. The invention provides the beauveria bassiana bh001 which is preserved in CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC No.17975. The biocontrol strain of the beauveria bassiana bh001 provided by the invention can be used for effectively preventing and treating curculio arakawai, has a good biocontrol potential, provides new resources for biological control on forest pests of the curculio arakawai, andalso provides help for protecting Mongolian oak seed resources.

The invention discloses a composition containing a microorganism complex. The composition containing the microorganism complex comprises, by weight, 1-2 parts of Bacillus cereus, 1-2 part sof thiamethoxam and 5-25 parts of Beauveria bassiana. The pesticide composition containing the microorganism complex can be processed to prepare a wettable powder, a water-dispersible granule, a missible oil, amicroemulsion, a suspending agent or a dispersible oil suspending agent, is used for controlling pests and diseases of rice, peanut, soybean, potato, citrus, beet, rape, pepper, cucumber and eggplant,and has the use of controlling Lepidoptera, Coleoptera, Homoptera and Diptera pests, underground pests and soil-borne bacterial diseases on vegetables. The pesticide composition containing the microorganism complex can reduce the use amount of pesticides and delay the drug resistance, has a better control effect than the single doses, and reduces the cost of farmers.