100 results about "Beauveria sp." patented technology

The invention relates to the technical field of microorganisms, in particular to a method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn and application thereof.The method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn comprises the following specific steps that S1, a test field is subjected to scribing treatment and divided into four test fields, each test field is divided into four ridges, the ridge width is 0.61 m, and the ridge width is 0.6 m; the ridge length is 15 m, the aisle between each cell is 2 m, and the front and rear protection rows of the test area are 10 m respectively; s2, a first area is a blank control area for normal planting of corn plants, a second area is a second area for drip irrigation of beauveria bassiana planting (Bb) for the corn plants, a third area is a third area for northern leaf blight inoculation planting (Et) for the corn plants, and a fourth area is a fourth area for drip irrigation of beauveria bassiana planting (Bb + Et) after northern leaf blight inoculation for the corn plants. Beauveria bassiana applied in a drip irrigation mode has good effects on corn growth and resistance.

The invention discloses an intelligent management method for medicinal bombyx batryticatus culture, and belongs to the technical field of intelligent management, and the method comprises the steps: placing silkworm chrysalis inoculated with beauveria bassiana in the same batch at a specimen collection point, and obtaining a monitoring data sequence, a monitoring image set and inoculation parameters of the beauveria bassiana corresponding to the specimen collection point in real time; identifying the silkworm chrysalis by the image identification model, outputting a silkworm chrysalis stiffness degree sequence, and calculating a silkworm chrysalis stiffness period; inputting all the sequence sets into a monitoring model for analysis, generating a state management model, generating a state estimation table corresponding to the specimen collection points, and constructing a state condition list of silkworm chrysalis; preprocessing all collected data of the silkworm chrysalis to be monitored, inputting the preprocessed data into the state management model, outputting a stiffness state, judging whether the stiffness state is qualified or not based on the state condition list, and repeating the step until the stiffness process of the silkworm chrysalis to be monitored is completed to form the medicinal stiff silkworm. According to the invention, the intelligence and accuracy of the breeding management of the medicinal stiff silkworms can be improved.

The invention relates to the technical field of microorganisms, and discloses a Beauveria bassiana strain Bb20240406 and an application of the Beauveria bassiana strain Bb20240406 in prevention and control of diseases and insect pests, and the preservation number of the Beauveria bassiana strain Bb20240406 is CGMCC No.41802. The strain has high insecticidal activity on ground pests and underground pests of peanuts, and has a good control effect; the strain has a strong inhibition effect on pathogenic bacteria of peanut fruit rot, root rot, southern blight and leaf diseases, and has a good field control effect. In addition, the influence of the strain on the population number of orius sauliginosus is lower than that of a conventional chemical pesticide. When the strain provided by the invention is applied to production, the dosage and the use frequency of chemical pesticides can be reduced, and the problems of pesticide residues of agricultural products and environmental pollution are solved; the influence on the number of natural enemy populations is reduced, the farmland ecological environment is protected, and ecological balance is maintained.

The invention relates to a preparation method of a plant disease control fungicide, which comprises the following steps: taking traditional Chinese medicines with an antibacterial effect as raw materials, and carrying out first-step fermentation by adopting Trichoderma harzianum; beauveria bassiana and Penicillium baylii are subjected to second-step fermentation, bacillus velezensis is subjected to third-step fermentation, fermentation liquor is collected, a filter cake is further subjected to reflux extraction, an extracting solution and the fermentation liquor are collected, combined and concentrated into clear paste, an ethanol solution is added, standing is carried out, supernate is taken, borneol, gelatin and cellulose acetate are added to prepare mixed liquor, ultrafiltration is carried out, ultrafiltrate is collected, and freeze-dried powder is obtained. The microbial inoculum required by the invention is obtained. According to the invention, specific traditional Chinese medicines are adopted, an excellent broad-spectrum antibacterial system is formed through specific microbial fermentation, and the antibacterial system is compounded with borneol, gelatin, cellulose acetate and other components to form the fungicide, so that the effectiveness duration and the effect taking rate of the fungicide on the surface of a plant are effectively improved, and efficient prevention and treatment on various diseases such as fungi, bacteria and the like are formed.

The invention discloses a bacterial strain suitable for large-scale production of high-quality stiff silkworm and application of the bacterial strain. The strain is a beauveria bassiana KW2 strain, and is preserved in Guangdong Microbial Culture Collection Center on November 19, 2024, and the preservation number is GDMCC NO: 65502. When the KW2 strain is used for producing stiff silkworms, the stiff silkworms are pink in color and then gradually fade, so that efficient sorting of the stiff silkworms is facilitated, the quality of the stiff silkworms can be better controlled, the labor cost can be better reduced, and the method is very suitable for large-scale production of the stiff silkworms. Meanwhile, hyphae on the surface of the stiff silkworm are oily and thick, less spore powder is produced, and production environment management and personnel health are facilitated. The KW2 strain has a silkworm death rate of 100%, the bombyx batryticatus is cylindrical and high in quality, and various indexes such as moisture and the like meet standards. Moreover, the content of beauveria bassiana and ammonium oxalate is relatively high, and the medicinal value is high. The KW2 strain has a very good application prospect in improving the large-scale safe production efficiency of high-quality stiff silkworms.

The invention mainly provides Beauveria bassiana ZABb200102 and application of the Beauveria bassiana ZABb200102, the Beauveria bassiana ZABb200102 is preserved in China General Microbiological Culture Collection Center on October 28, 2024, and the preservation number of the Beauveria bassiana ZABb200102 is CGMCC (China General Microbiological Culture Collection Center) No. 41557. The strain is separated from sick lepidoptera cacalidae larva bodies for the first time, and it is found through toxicity determination that the strain has high pathogenicity to tomato leaf miner larvae. In addition, the strain can also be colonized in tomatoes, so that the growth of the tomatoes is promoted, and the resistance of the tomatoes to pests is indirectly improved. Therefore, the strain can be widely applied to development of tomato bacterial fertilizer and green prevention and control of tomato leaf miner which is an important tomato pest.

The present invention relates to a graphene oxide - Beauveria bassiana complex and its application in pest control and improving crop growth. The invention provides a graphene oxide - Beauveria bassiana complex, and optimizes the ratio of graphene oxide to Beauveria bassiana. Graphene oxide at a concentration of 20 μg / ml - 100 μg / ml has a UV - protecting effect on Beauveria bassiana spores. The experimental results show that the graphene oxide - Beauveria bassiana provided in this application can significantly improve the control effect of Beauveria bassiana on pests, and the seed soaking treatment can also significantly improve the growth indexes of crops such as plant height, leaf length, leaf width and leaf area, having unexpected technical effects and very good agricultural application value.

The present invention provides a dsRNA targeting the BcPfy1 gene, a Beauveria bassiana engineered strain Bb-dsPfy1, and their applications. The Beauveria bassiana engineered strain Bb-dsPfy1 uses the Beauveria bassiana QSE-F1 strain as the chassis strain, and this strain can colonize the tomato leaves. The dsRNA is a double-stranded RNA fragment designed with the Botrytis cinerea BcPfy1 gene as the target, which has the functions of interfering with the expression of the BcPfy1 gene and inhibiting the infection of Botrytis cinerea. This fragment is obtained by in vitro synthesis or synthesized in the QSE-F1 strain through two target gene sequences with the same sequence and opposite directions. The engineered strain Bb-dsPfy1 is obtained by using the ATMT technique to transfer a vector containing the target gene sequence into the Beauveria bassiana QSE-F1 strain, and it is an engineered strain capable of synthesizing dsRNA targeting BcPfy1. It is demonstrated by experiments in the present invention that the engineered strain can effectively interfere with the expression of the target gene BcPfy1 and has a significant control effect on the occurrence of tomato gray mold. This engineered strain can be used to prepare biological agents for controlling gray mold and has good market application prospects.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The present disclosure relates to a Beauveria bassiana KNU-101 strain. The Beauveria bassiana KNU-101 strain is entomopathogenic, exhibits an excellent insecticidal effect on controlling thrips, which are soil pests that harm crops, and also has excellent sporulation ability. Thus, the Beauveria bassiana KNU-101 strain may be effectively used as an environmentally friendly means for controlling pests.

The invention belongs to the technical field of high-temperature koji making and wine brewing, and particularly relates to traditional Chinese medicine-containing anti-koji high-temperature yeast and a preparation method thereof, the raw materials comprise 90-100 parts of wheat, 8-10 parts of mother yeast, 2-4 parts of traditional Chinese medicines, and 0.5-1.5 parts of a complex microbial inoculant; wherein the traditional Chinese medicine comprises at least one of tripterygium wilfordii, radix sophorae flavescentis and mint; the complex microbial inoculants comprise bacillus thuringiensis, bacillus sphaericus, beauveria bassiana and metarhizium anisopliae. The traditional Chinese medicines and the complex microbial inoculant are added into the raw materials, so that the high-temperature yeast has the effects of avoiding, refusal to eat and killing the stomach on yeast insects on the premise of not generating toxic substances to influence food safety, and can also endow the high-temperature yeast with special traditional Chinese medicine fragrance, and the content of flavor substances in the high-temperature yeast is increased.

The invention belongs to the technical field of pest control, and relates to a red imported fire ant control agent taking compound microorganisms as raw materials and a preparation method thereof, and the preparation method specifically comprises the following steps: step 1, culturing beauveria bassiana to obtain beauveria bassiana spore powder; step 2, culturing bacillus amyloliquefaciens and bacillus subtilis to obtain a compound bacteria metabolite; 3, modifying and roasting diatomite to obtain an active carrier; step 4, mixing and shearing the beauveria bassiana spore powder, the compound bacteria metabolite, the active carrier, the plant source synergist, the slow-release protective agent, the surfactant, the stabilizer, the buffer solution and the water to obtain the solenopsis invicta prevention and control agent.

The present application belongs to the technical field of preparation of white-backed caterpillar. More particularly, it relates to a Beauveria bassiana agent for greatly improving the time concentration of death of domestic silkworm and a high-quality white-backed caterpillar production method. The present application provides a Beauveria bassiana agent containing a strain of Beauveria bassiana and a fermentation product of another strain of Beauveria bassiana without bacteria bodies. The use of the agent in the present application to infect domestic silkworm can shorten the time of complete death of domestic silkworm, and concentrate the death time of domestic silkworm at the 6th day after inoculation, with a time span of only 24 hours, which helps to improve the production efficiency of white-backed caterpillar, effectively reduces the labor of feeding and picking silkworms during the death stage, and reduces the production cost. In addition, the method for producing white-backed caterpillar in the present application can achieve good infection effect by spraying the agent once, reducing the process of white-backed caterpillar production. And the white-backed caterpillar produced by the method in the present application is uniform in size, the consistency of product quality parameters is good, and the quality is stable, which helps to improve the clinical application effect.

The invention discloses a method for cultivating and producing white muscardine silkworms by using 5-year-old silkworm larvae as a culture medium. The method specifically comprises the following steps: (1) quality improvement and optimization of a mother strain of beauveria bassiana; (2) carrying out stress stimulation culture on the beauveria bassiana solid-liquid mixed leavening agent; (3) carrying out inoculation treatment on a culture medium of 5-year-old silkworm larvae; and (4) white muscardine silkworm spawn running management. According to the method, the silkworm larvae of 5 years old are used as the culture medium to breed and produce the silkworm larva, a new way for preparing the medicinal silkworm larva is provided, and the silkworm larvae of 5 years old can be utilized and converted into the silkworm larva to accumulate nutrition and bioactive components to the greatest extent; the method is not limited by weak infection lethal ability of the beauveria bassiana strain and strong resistance of the hybridized combined silkworm variety, the beauveria bassiana with high bioactive component attribute and the large hybridized combined silkworm variety with rich nutrition and mass can be fully compatible, the quality of the silkworm larva medicinal material is ensured, meanwhile, the yield is remarkably improved, and the method is stable, efficient and wide in development prospect.

The invention discloses a composite agent for preventing and treating rice blast, and belongs to the technical field of crop sterilization agents. The agent comprises a fungicide, an antiviral agent, a disease and pest resistant agent and a compound microbial agent according to a mass ratio. Wherein the fungicide is mainly formed by mixing iprodione, spinosad and cyprodinil, the antiviral agent is formed by mixing glucosamine, ribonuclease and beta-carotene, the disease and pest resistant agent is formed by mixing glyphosate and glufosinate-ammonium, and the compound microbial agent is formed by compounding beauveria bassiana and beauveria bassiana. Through reasonable matching of the components, the synergistic effect is achieved, the resistance of the components is reduced, and the pesticide effect is improved. In addition, the composite agent not only can effectively control the occurrence of rice blast, but also can improve the resistance of plants to the rice blast by enhancing the stress resistance and immune function of the plants, thereby achieving the purpose of prevention and treatment.

The invention belongs to the technical field of biological control, and particularly relates to beauveria bassiana TYW001 and application thereof. The invention provides beauveria bassiana TYW001, the Latin name of the beauveria bassiana TYW001 is Beauveria bassiana, the beauveria bassiana TYW001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 30, 2025, and the preservation number is CGMCC NO.42149. The strain not only can resist high temperature and drought, but also can resist high temperature and drought, but also can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and can resist high temperature the soybean megalurothrips can be effectively prevented and treated; moreover, the beauveria bassiana TYW001 can enhance the predation effect of the orius minutus on the megalurothrips megalurothrips, and a reliable green prevention and control technology is provided for prevention and control of the megalurothrips megalurothrips.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The present invention belongs to the field of pesticides, and specifically relates to a pesticide composition for controlling wheat aphids. The present invention provides a Beauveria bassiana strain HFW-05, which is pathogenic to wheat aphids. When combined with sulfoxaflor, the composition exhibits significant synergistic effects, improving control efficacy, significantly reducing the dosage of a single chemical pesticide, reducing environmental pollution, and delaying the development of pesticide resistance in target pests. The composition has promising application prospects.

The invention relates to the technical field of prevention and control of bactrocera cucurbitae, in particular to high-temperature-resistant beauveria bassiana WZS-5 and application thereof to prevention and control of bactrocera cucurbitae, and the preservation number of the high-temperature-resistant beauveria bassiana WZS-5 is GDMCCNO: 66358. The optimal growth temperature of the strain is 28-30 DEG C, and the strain still keeps certain growth and reproduction capability at the high temperature of 35 DEG C. Biological assay results show that under the conditions that the temperature is 30 DEG C and the relative humidity is 70%, the prevention and treatment effect of the strain on beauveria bassiana is remarkably better than that of common beauveria bassiana, and the accumulative death rate of bactrocera cucurbitae adults under the concentration of 1 * 10 < 9 > spores / mL reaches 89%. Results show that the strain has excellent high-temperature adaptability and strong pathogenic activity, can efficiently control bactrocera cucurbitae populations in tropical and subtropical high-temperature environments, and provides new microbial resources and technical approaches for green prevention and control of fruit and vegetable pests.

The invention provides recombinant beauveria bassiana for catalyzing a high-concentration substrate as well as a construction method and application of the recombinant beauveria bassiana. Based on a metabolic engineering and efflux transport engineering strategy, cytochrome P450 enzyme or a transmembrane transport protein coding gene selected from an NPF protein family and an SWEET protein family is constructed into beauveria bassiana for producing R-2-(4-hydroxyphenoxy) propionic acid; the recombinant beauveria bassiana capable of stably catalyzing the conversion of the high-concentration R-2-phenoxypropionic acid into the R-2-(4-hydroxyphenoxy) propionic acid is constructed. Compared with a control strain, an experimental group has the advantages that the tolerance to the concentration of R-2-phenoxypropionic acid is obviously improved, and the fermentation period is shortened. The construction method provided by the invention provides an important target for transforming high-concentration R-2-phenoxypropionic acid into R-2-(4-hydroxyphenoxy) propionic acid under the catalysis of the transformed beauveria bassiana, and has an industrial application prospect.

The invention discloses a method for synthesizing R-2-(4-hydroxyphenoxy) propionic acid by submerged culture of beauveria bassiana, and provides a submerged culture method of R-2-(4-hydroxyphenoxy) propionic acid, which comprises the following steps: adding soybean oil and a phase transfer catalyst; the phase transfer catalyst is prepared from one or more of cyclodextrin, tetrabutylammonium bromide, 1-butyl-3-methylimidazolium hexafluorophosphate and polyethylene glycol 1000. By adopting a supplementary material synergistic method, the yield of the R-2-(4-hydroxyphenoxy) propionic acid produced by using the beauveria bassiana ZJB23323 as a production strain through deep culture is remarkably improved.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.

The invention relates to the technical field of microorganisms, in particular to beauveria bassiana QZ and application thereof. The collection number of the beauveria bassiana QZ is GDMCC No: 66669. The beauveria bassiana QZ provided by the invention has strong pathogenicity to coffee berry bark beetles, the strain is modified to express and deliver double-stranded RNA-dsDorsal, the pathogenicity is stronger, laboratory data shows that the fatality rate is almost 100% within 8 days, and the lethal ability is improved by 20% compared with that of a non-transgenic strain.

The invention relates to the technical field of beauveria bassiana microbial preparations, in particular to application and a method of a beauveria bassiana microbial preparation in tomato preservation. 7-11% of a composite protective agent; and 0.5%-1.5% of a synergist. The three-level application scheme of dip-coating, spraying and modified atmosphere packaging is designed for normal temperature, low temperature and cold chain, and the refreshing time is prolonged by 3-5 times; through strain improvement, preparation innovation and application technology optimization, the beauveria bassiana is developed into the special fresh-keeping agent for tomatoes for the first time.

The invention belongs to the technical field of biology, and particularly relates to a primer group for detecting spores and hyphae of beauveria bassiana and application of the primer group. The primer group comprises a primer for detecting spores of the beauveria bassiana and a primer for detecting hyphae of the beauveria bassiana; the primer for detecting the beauveria bassiana spores is a primer for detecting an A5201 gene of the beauveria bassiana, and the nucleotide sequence of the A5201 gene is as shown in SEQ ID NO.7; the primer for detecting the beauveria bassiana hypha is a primer for detecting an A1176 gene of the beauveria bassiana, and the nucleotide sequence of the A1176 gene is shown as SEQ ID NO. 1. The nucleotide sequence of the primer for detecting the beauveria bassiana A5201 gene is as follows: an upstream primer 5 '-ATGAGCACTTTGAGCTTCGAC-3', and a downstream primer 5 '-TTATCTTCTCGTGGGCTGTC-3', and the nucleotide sequence of the primer for detecting the beauveria bassiana A5201 gene is as follows: the upstream primer 5 '-ATGAGCACTTTGAGCTGTC-3'; the nucleotide sequence of the primer for detecting the beauveria bassiana A1176 gene is as follows: an upstream primer 5 '-ATGAAGACTGCTGTTGGTATTGTTT-3', and a downstream primer 5 '-TCATATTCTCGCGAGAACGAC-3'. The primer for detecting the beauveria bassiana A1176 gene is characterized in that the upstream primer 5 '- The method is suitable for RT-qPCR detection, the operation is simple, and the hyphae and spores of the beauveria bassiana are separated from the counting aspect for the first time.

The utility model discloses a beauveria bassiana strain marking and storing device, which relates to the technical field of storing devices, and comprises an ultra-low temperature refrigerator for ultra-low temperature refrigeration of beauveria bassiana strains; the placing box is movably arranged in the ultralow-temperature refrigerator and used for placing beauveria bassiana strains; the adjusting mechanism is arranged in the ultralow-temperature refrigerator and used for adjusting the position of the containing box and changing the space layout in the ultralow-temperature refrigerator; according to the beauveria bassiana strain marking device, due to the arrangement of the containing box, the adjusting mechanism and the marking mechanism, the containing box can be adjusted, the adjusted position is fixed, the beauveria bassiana strains can be conveniently placed, and the beauveria bassiana strain marking device is convenient to use and high in practicability. The beauveria bassiana strain marking device can be repeatedly used for marking different batches of beauveria bassiana strains, only the beauveria bassiana strains on one placing box need to be marked at a time, and operation is convenient and fast.

Disclosed herein are compositions comprising A) compound of Formula One (F1) or Formula Two (F2); and B) biological agent selected from the group consisting of Bacillus species, Baclovirus species, Pseudomonas species, Beauveria species, Mycobacterium species, Mycorrhizal fungi, Trichoderma species, and Metarhizium species.

The invention discloses a culture box for efficiently culturing beauveria bassiana conidial powder and a culture method.The culture box comprises a transparent box body, a cover body is arranged at the upper end of the box body, a sealing ring matched with the box body for use is arranged at the lower end of the cover body, an air hole is formed in the cover body, and a supporting net is arranged in the air hole; the bottom of the box body is provided with air holes, the air holes are provided with air-permeable sealing films installed on the supporting net, the length and the width of the box body are 20-25 cm, the height of the box body is 5-8 cm, the diameter of the air holes is 2-3 cm, and the bottom of the box body is in a continuous wave shape from left to right. When the culture box is used for culturing beauveria bassiana, pollution can be effectively avoided, the culture box has the advantages of being easy to operate, low in equipment cost and low in technical requirement, and the wavy bottom of the culture box can increase the bottom area, improve the ventilation effect and guarantee balance of the temperature of the bottom in the culture box.

The invention discloses two odontotermes formosanus OfCTLs genes and application of dsRNA of the odontotermes formosanus OfCTLs genes. Wherein the two odontotermes formosanus OfCTLs genes are an OfCTL-S3 gene and an OfCTL-S15 gene respectively, and the corresponding nucleotide sequences of the odontotermes formosanus OfCTL-S3 gene and the OfCTL-S15 gene are SEQ ID NO: 1 and SEQ ID NO: 2 respectively. Recombinant OfCTL-S3 and OfCTL-S15 proteins are obtained through a prokaryotic expression system, and an in-vitro antibacterial test proves that the OfCTL-S3 and OfCTL-S15 proteins have antibacterial activity and agglutination effects on beauveria bassiana, gram-positive bacterium bacillus thuringiensis and gram-negative bacterium serratia marcescens in the presence of calcium ions. According to the invention, an odontotermes formosanus OfCTLs RNA interference system carried out by using a feeding method is established, and an obvious gene silencing effect is obtained by feeding odontotermes formosanus. The dsRNA of the odontotermes formosanus OfCTLs is combined with the biocontrol bacteria, so that the control effect of the biocontrol bacteria can be remarkably improved.