80 results about "Beauveria sp." patented technology

The invention relates to a preparation method of a plant disease control fungicide, which comprises the following steps: taking traditional Chinese medicines with an antibacterial effect as raw materials, and carrying out first-step fermentation by adopting Trichoderma harzianum; beauveria bassiana and Penicillium baylii are subjected to second-step fermentation, bacillus velezensis is subjected to third-step fermentation, fermentation liquor is collected, a filter cake is further subjected to reflux extraction, an extracting solution and the fermentation liquor are collected, combined and concentrated into clear paste, an ethanol solution is added, standing is carried out, supernate is taken, borneol, gelatin and cellulose acetate are added to prepare mixed liquor, ultrafiltration is carried out, ultrafiltrate is collected, and freeze-dried powder is obtained. The microbial inoculum required by the invention is obtained. According to the invention, specific traditional Chinese medicines are adopted, an excellent broad-spectrum antibacterial system is formed through specific microbial fermentation, and the antibacterial system is compounded with borneol, gelatin, cellulose acetate and other components to form the fungicide, so that the effectiveness duration and the effect taking rate of the fungicide on the surface of a plant are effectively improved, and efficient prevention and treatment on various diseases such as fungi, bacteria and the like are formed.

The invention mainly provides Beauveria bassiana ZABb200102 and application of the Beauveria bassiana ZABb200102, the Beauveria bassiana ZABb200102 is preserved in China General Microbiological Culture Collection Center on October 28, 2024, and the preservation number of the Beauveria bassiana ZABb200102 is CGMCC (China General Microbiological Culture Collection Center) No. 41557. The strain is separated from sick lepidoptera cacalidae larva bodies for the first time, and it is found through toxicity determination that the strain has high pathogenicity to tomato leaf miner larvae. In addition, the strain can also be colonized in tomatoes, so that the growth of the tomatoes is promoted, and the resistance of the tomatoes to pests is indirectly improved. Therefore, the strain can be widely applied to development of tomato bacterial fertilizer and green prevention and control of tomato leaf miner which is an important tomato pest.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The invention belongs to the technical field of high-temperature koji making and wine brewing, and particularly relates to traditional Chinese medicine-containing anti-koji high-temperature yeast and a preparation method thereof, the raw materials comprise 90-100 parts of wheat, 8-10 parts of mother yeast, 2-4 parts of traditional Chinese medicines, and 0.5-1.5 parts of a complex microbial inoculant; wherein the traditional Chinese medicine comprises at least one of tripterygium wilfordii, radix sophorae flavescentis and mint; the complex microbial inoculants comprise bacillus thuringiensis, bacillus sphaericus, beauveria bassiana and metarhizium anisopliae. The traditional Chinese medicines and the complex microbial inoculant are added into the raw materials, so that the high-temperature yeast has the effects of avoiding, refusal to eat and killing the stomach on yeast insects on the premise of not generating toxic substances to influence food safety, and can also endow the high-temperature yeast with special traditional Chinese medicine fragrance, and the content of flavor substances in the high-temperature yeast is increased.

The invention belongs to the technical field of pest control, and relates to a red imported fire ant control agent taking compound microorganisms as raw materials and a preparation method thereof, and the preparation method specifically comprises the following steps: step 1, culturing beauveria bassiana to obtain beauveria bassiana spore powder; step 2, culturing bacillus amyloliquefaciens and bacillus subtilis to obtain a compound bacteria metabolite; 3, modifying and roasting diatomite to obtain an active carrier; step 4, mixing and shearing the beauveria bassiana spore powder, the compound bacteria metabolite, the active carrier, the plant source synergist, the slow-release protective agent, the surfactant, the stabilizer, the buffer solution and the water to obtain the solenopsis invicta prevention and control agent.

The present application belongs to the technical field of preparation of white-backed caterpillar. More particularly, it relates to a Beauveria bassiana agent for greatly improving the time concentration of death of domestic silkworm and a high-quality white-backed caterpillar production method. The present application provides a Beauveria bassiana agent containing a strain of Beauveria bassiana and a fermentation product of another strain of Beauveria bassiana without bacteria bodies. The use of the agent in the present application to infect domestic silkworm can shorten the time of complete death of domestic silkworm, and concentrate the death time of domestic silkworm at the 6th day after inoculation, with a time span of only 24 hours, which helps to improve the production efficiency of white-backed caterpillar, effectively reduces the labor of feeding and picking silkworms during the death stage, and reduces the production cost. In addition, the method for producing white-backed caterpillar in the present application can achieve good infection effect by spraying the agent once, reducing the process of white-backed caterpillar production. And the white-backed caterpillar produced by the method in the present application is uniform in size, the consistency of product quality parameters is good, and the quality is stable, which helps to improve the clinical application effect.

The invention discloses a method for cultivating and producing white muscardine silkworms by using 5-year-old silkworm larvae as a culture medium. The method specifically comprises the following steps: (1) quality improvement and optimization of a mother strain of beauveria bassiana; (2) carrying out stress stimulation culture on the beauveria bassiana solid-liquid mixed leavening agent; (3) carrying out inoculation treatment on a culture medium of 5-year-old silkworm larvae; and (4) white muscardine silkworm spawn running management. According to the method, the silkworm larvae of 5 years old are used as the culture medium to breed and produce the silkworm larva, a new way for preparing the medicinal silkworm larva is provided, and the silkworm larvae of 5 years old can be utilized and converted into the silkworm larva to accumulate nutrition and bioactive components to the greatest extent; the method is not limited by weak infection lethal ability of the beauveria bassiana strain and strong resistance of the hybridized combined silkworm variety, the beauveria bassiana with high bioactive component attribute and the large hybridized combined silkworm variety with rich nutrition and mass can be fully compatible, the quality of the silkworm larva medicinal material is ensured, meanwhile, the yield is remarkably improved, and the method is stable, efficient and wide in development prospect.

The invention discloses a composite agent for preventing and treating rice blast, and belongs to the technical field of crop sterilization agents. The agent comprises a fungicide, an antiviral agent, a disease and pest resistant agent and a compound microbial agent according to a mass ratio. Wherein the fungicide is mainly formed by mixing iprodione, spinosad and cyprodinil, the antiviral agent is formed by mixing glucosamine, ribonuclease and beta-carotene, the disease and pest resistant agent is formed by mixing glyphosate and glufosinate-ammonium, and the compound microbial agent is formed by compounding beauveria bassiana and beauveria bassiana. Through reasonable matching of the components, the synergistic effect is achieved, the resistance of the components is reduced, and the pesticide effect is improved. In addition, the composite agent not only can effectively control the occurrence of rice blast, but also can improve the resistance of plants to the rice blast by enhancing the stress resistance and immune function of the plants, thereby achieving the purpose of prevention and treatment.

The invention belongs to the technical field of biological control, and particularly relates to beauveria bassiana TYW001 and application thereof. The invention provides beauveria bassiana TYW001, the Latin name of the beauveria bassiana TYW001 is Beauveria bassiana, the beauveria bassiana TYW001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 30, 2025, and the preservation number is CGMCC NO.42149. The strain not only can resist high temperature and drought, but also can resist high temperature and drought, but also can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and can resist high temperature the soybean megalurothrips can be effectively prevented and treated; moreover, the beauveria bassiana TYW001 can enhance the predation effect of the orius minutus on the megalurothrips megalurothrips, and a reliable green prevention and control technology is provided for prevention and control of the megalurothrips megalurothrips.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The present invention belongs to the field of pesticides, and specifically relates to a pesticide composition for controlling wheat aphids. The present invention provides a Beauveria bassiana strain HFW-05, which is pathogenic to wheat aphids. When combined with sulfoxaflor, the composition exhibits significant synergistic effects, improving control efficacy, significantly reducing the dosage of a single chemical pesticide, reducing environmental pollution, and delaying the development of pesticide resistance in target pests. The composition has promising application prospects.

The invention relates to the technical field of prevention and control of bactrocera cucurbitae, in particular to high-temperature-resistant beauveria bassiana WZS-5 and application thereof to prevention and control of bactrocera cucurbitae, and the preservation number of the high-temperature-resistant beauveria bassiana WZS-5 is GDMCCNO: 66358. The optimal growth temperature of the strain is 28-30 DEG C, and the strain still keeps certain growth and reproduction capability at the high temperature of 35 DEG C. Biological assay results show that under the conditions that the temperature is 30 DEG C and the relative humidity is 70%, the prevention and treatment effect of the strain on beauveria bassiana is remarkably better than that of common beauveria bassiana, and the accumulative death rate of bactrocera cucurbitae adults under the concentration of 1 * 10 < 9 > spores / mL reaches 89%. Results show that the strain has excellent high-temperature adaptability and strong pathogenic activity, can efficiently control bactrocera cucurbitae populations in tropical and subtropical high-temperature environments, and provides new microbial resources and technical approaches for green prevention and control of fruit and vegetable pests.

The invention provides recombinant beauveria bassiana for catalyzing a high-concentration substrate as well as a construction method and application of the recombinant beauveria bassiana. Based on a metabolic engineering and efflux transport engineering strategy, cytochrome P450 enzyme or a transmembrane transport protein coding gene selected from an NPF protein family and an SWEET protein family is constructed into beauveria bassiana for producing R-2-(4-hydroxyphenoxy) propionic acid; the recombinant beauveria bassiana capable of stably catalyzing the conversion of the high-concentration R-2-phenoxypropionic acid into the R-2-(4-hydroxyphenoxy) propionic acid is constructed. Compared with a control strain, an experimental group has the advantages that the tolerance to the concentration of R-2-phenoxypropionic acid is obviously improved, and the fermentation period is shortened. The construction method provided by the invention provides an important target for transforming high-concentration R-2-phenoxypropionic acid into R-2-(4-hydroxyphenoxy) propionic acid under the catalysis of the transformed beauveria bassiana, and has an industrial application prospect.

The invention discloses a method for synthesizing R-2-(4-hydroxyphenoxy) propionic acid by submerged culture of beauveria bassiana, and provides a submerged culture method of R-2-(4-hydroxyphenoxy) propionic acid, which comprises the following steps: adding soybean oil and a phase transfer catalyst; the phase transfer catalyst is prepared from one or more of cyclodextrin, tetrabutylammonium bromide, 1-butyl-3-methylimidazolium hexafluorophosphate and polyethylene glycol 1000. By adopting a supplementary material synergistic method, the yield of the R-2-(4-hydroxyphenoxy) propionic acid produced by using the beauveria bassiana ZJB23323 as a production strain through deep culture is remarkably improved.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.

The invention relates to the technical field of microorganisms, in particular to beauveria bassiana QZ and application thereof. The collection number of the beauveria bassiana QZ is GDMCC No: 66669. The beauveria bassiana QZ provided by the invention has strong pathogenicity to coffee berry bark beetles, the strain is modified to express and deliver double-stranded RNA-dsDorsal, the pathogenicity is stronger, laboratory data shows that the fatality rate is almost 100% within 8 days, and the lethal ability is improved by 20% compared with that of a non-transgenic strain.

The invention relates to the technical field of beauveria bassiana microbial preparations, in particular to application and a method of a beauveria bassiana microbial preparation in tomato preservation. 7-11% of a composite protective agent; and 0.5%-1.5% of a synergist. The three-level application scheme of dip-coating, spraying and modified atmosphere packaging is designed for normal temperature, low temperature and cold chain, and the refreshing time is prolonged by 3-5 times; through strain improvement, preparation innovation and application technology optimization, the beauveria bassiana is developed into the special fresh-keeping agent for tomatoes for the first time.

Disclosed herein are compositions comprising A) compound of Formula One (F1) or Formula Two (F2); and B) biological agent selected from the group consisting of Bacillus species, Baclovirus species, Pseudomonas species, Beauveria species, Mycobacterium species, Mycorrhizal fungi, Trichoderma species, and Metarhizium species.

The invention discloses a culture box for efficiently culturing beauveria bassiana conidial powder and a culture method.The culture box comprises a transparent box body, a cover body is arranged at the upper end of the box body, a sealing ring matched with the box body for use is arranged at the lower end of the cover body, an air hole is formed in the cover body, and a supporting net is arranged in the air hole; the bottom of the box body is provided with air holes, the air holes are provided with air-permeable sealing films installed on the supporting net, the length and the width of the box body are 20-25 cm, the height of the box body is 5-8 cm, the diameter of the air holes is 2-3 cm, and the bottom of the box body is in a continuous wave shape from left to right. When the culture box is used for culturing beauveria bassiana, pollution can be effectively avoided, the culture box has the advantages of being easy to operate, low in equipment cost and low in technical requirement, and the wavy bottom of the culture box can increase the bottom area, improve the ventilation effect and guarantee balance of the temperature of the bottom in the culture box.

The invention discloses two odontotermes formosanus OfCTLs genes and application of dsRNA of the odontotermes formosanus OfCTLs genes. Wherein the two odontotermes formosanus OfCTLs genes are an OfCTL-S3 gene and an OfCTL-S15 gene respectively, and the corresponding nucleotide sequences of the odontotermes formosanus OfCTL-S3 gene and the OfCTL-S15 gene are SEQ ID NO: 1 and SEQ ID NO: 2 respectively. Recombinant OfCTL-S3 and OfCTL-S15 proteins are obtained through a prokaryotic expression system, and an in-vitro antibacterial test proves that the OfCTL-S3 and OfCTL-S15 proteins have antibacterial activity and agglutination effects on beauveria bassiana, gram-positive bacterium bacillus thuringiensis and gram-negative bacterium serratia marcescens in the presence of calcium ions. According to the invention, an odontotermes formosanus OfCTLs RNA interference system carried out by using a feeding method is established, and an obvious gene silencing effect is obtained by feeding odontotermes formosanus. The dsRNA of the odontotermes formosanus OfCTLs is combined with the biocontrol bacteria, so that the control effect of the biocontrol bacteria can be remarkably improved.

The application discloses a Beauveria bassiana strain SL-066 and application thereof, and relates to the technical field of biological strains. The strain provided by the application is preserved in the China General Microbiological Culture Collection Center on August 8, 2022, and the preservation number is CGMCC No. 40269. The Beauveria bassiana strain SL-066 provided by the application can effectively prevent and control various rice pests, is a Beauveria bassiana strain capable of preventing and controlling the coleopteran pest Curculionidae (rice water weevil adult), the coleopteran pest Chrysomelidae (rice water weevil larva) and the lepidopteran pest Chilo suppressalis (Chilo suppressalis larva), and has a good prevention and control effect on the three common pests on rice.

The application provides a Beauveria bassiana strain Bb-YY for preventing and controlling various diseases and pests, a derivative product and application thereof, and belongs to the technical field of functional microorganisms. Beauveria bassiana The strain Bb-YY is screened from the rhizosphere soil of a peanut healthy plant planted in a serious continuous cropping obstacle plot, and is identified as Beauveria bassiana (Bb) by molecular identification, has high efficient prevention and control effects on white grubs, peanut fruit rot pathogens and peanut root rot pathogens, and has certain inhibition effects on Bacterospora coriacea causing tobacco black foot disease and Pyricularia oryzae causing rice blast. It can be seen that the Bb-YY strain can also play a prevention and control effect on tobacco, rice and other crops in addition to peanut planting, and can effectively reduce the use of chemical pesticides, and provides a strong support for green prevention and control of peanut diseases and pests.

The invention belongs to the technical field of stiff silkworm preparation. More specifically, the invention relates to a beauveria bassiana microbial inoculum capable of greatly improving silkworm death time concentration and a high-quality stiff silkworm production method. The invention provides a beauveria bassiana microbial inoculum which contains a strain of beauveria bassiana and a fermentation product of another strain of beauveria bassiana, wherein the fermentation product is obtained by removing thalli. When the bombyx mori is infected by the microbial inoculum, the time that all bombyx mori is dead can be shortened, the bombyx mori dead time is concentrated on the sixth day after inoculation, the time span is only 24 h, the production efficiency of bombyx mori can be improved, the labor for feeding and picking silkworms in the dead stage is effectively reduced, and the production cost is reduced; in addition, according to the method for producing the bombyx batryticatus, a very good infection effect can be achieved by spraying the fungicide once for inoculation, and the working procedures for producing the bombyx batryticatus are reduced. And the bombyx batryticatus prepared by the method for producing the bombyx batryticatus is uniform in size, good in consistency of product quality parameters and stable in quality, and the clinical application effect can be improved.

The invention relates to the technical field of agricultural microorganism application, in particular to a beauveria bassiana Bb455 strain with efficient nitrogen fixation capacity and application of the strain in promotion of corn growth, and the beauveria bassiana Bb455 strain is suitable for green planting of grain crops such as corn and the like, can reduce application of chemical nitrogen fertilizer and can promote sustainable development of agricultural ecology.

The present invention discloses an intelligent management method for medicinal silkworm farming, which belongs to the field of intelligent management technology. The method comprises the following steps: placing silkworm pupae inoculated with Beauveria bassiana from the same batch at a specimen collection point, acquiring a monitoring data sequence, a monitoring image set, and Beauveria bassiana inoculation parameters corresponding to the specimen collection point in real time; using an image recognition model to identify the silkworm pupae, outputting a sequence of the degree of rigidity of the silkworm pupae, and calculating the rigidity period of the silkworm pupae; inputting all sequence sets into a monitoring model for analysis, generating a state management model, generating a state estimation table corresponding to the specimen collection point, and constructing a state condition list for the silkworm pupae; pre-processing all collected data of the silkworm pupae to be monitored and inputting them into the state management model, outputting a rigidity state, judging whether the rigidity state is qualified based on the state condition list, and repeating this step until the silkworm pupae to be monitored complete the rigidity process and form medicinal silkworm pupae. The present invention can improve the intelligence and accuracy of medicinal silkworm farming management.

The invention belongs to the technical field of biocontrol microorganism genetic engineering, and particularly relates to an sfgA gene for regulating and controlling growth and development of beauveria bassiana and application of the sfgA gene. The invention discloses an sfgA gene for regulating and controlling growth and development of beauveria bassiana, the nucleotide sequence of the sfgA gene is as shown in SEQ ID NO.1, and the amino acid sequence of encoded protein of the sfgA gene is as shown in SEQ ID NO.2. By systematically researching the effect of the development signal protein sfgA in the asexual spore production process of the beauveria bassiana and the regulation and control mechanism of the development signal protein sfgA, the growth and development of the beauveria bassiana can be regulated and controlled. A theoretical basis is laid for researching a filamentous fungus asexual sporulation molecular mechanism, and a new scientific basis and gene resources are provided for mining and genetic improvement of biocontrol potentials of biocontrol fungi.

The invention relates to the technical field of biological control, in particular to a Beauveria bassiana and attractant compounding and screening method, which comprises the following steps: collecting fruit flies with Beauveria bassiana colonies and obtaining a plurality of Beauveria bassiana strains; obtaining a biological prevention and control evaluation value of the strain through indoor biological testing, and judging whether the performance of the strain meets a preset standard or not according to the biological prevention and control evaluation value; the strains with the performance meeting the preset standard are compounded with a plurality of attractants, and a plurality of mixed agents are obtained; the spore germination rate of each mixed agent is obtained through detection, and the optimal attractant is determined; according to an attraction effect evaluation value obtained through the attraction test, whether attraction meets a preset standard or not is judged; the spore concentration is increased under the condition that the attraction does not meet the preset standard, or the reason that the attraction does not meet the preset standard is determined according to the survival bacterium carrying rate, and the attraction temperature or the attraction duration is adjusted. The biological control effect is improved.

This invention relates to a triple nano-PCR detection method for silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus, belonging to the field of molecular diagnostic technology. Addressing the technical problem of existing silkworm disease diagnostic methods' inability to simultaneously, efficiently, and sensitively identify these three commonly mixed infection pathogens, this invention provides an integrated solution. The key technical points include extracting DNA from the sample to be tested, using this DNA as a template, performing nano-PCR amplification in the same reaction system using a specific primer set, and subsequently detecting the amplification products by agarose gel electrophoresis. The infection status of silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus is determined based on the specific bands at 733 bp, 230 bp, and 860 bp, respectively. This method is mainly used for the rapid, simultaneous diagnosis and identification of mixed infections of microsporidia, Beauveria bassiana, and hemorrhagic septicemia during silkworm rearing.

The present invention provides stable, non-aqueous biopesticides. The present invention relates to biopestic formulations of entomopathogenic fungi or their spores. In particular, the present invention discloses oil dispersions containing entomopathogenic fungi or their spores, especially Beauveria bassiana. The present invention also relates to methods for preparing the formulations and their use.