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51 results about "Death time" patented technology

Network clustering method of wireless sensor based on fixed cluster heads

The invention discloses a network clustering method of a wireless sensor based on fixed cluster heads, suitable to a network topological structure of a hierarchical wireless sensor. In the method, nodes with relatively larger energy are used as fixed cluster heads to form a static cluster, and a method for establishing an intra-cluster data forwarding path is provided for solving the problem of long span from a first node death time to a last node death time. Under the condition of the same network energy consumption, in compression with an LEACH algorithm, the method can greatly improve the utilization efficiency of network energy and effectively reduce the mean energy consumption of the network in every round without adding any hardware equipment, and thus the method has popularization and application values.
Owner:NAVAL AERONAUTICAL & ASTRONAUTICAL UNIV PLA

Beauveria bassiana strain having high pathogenicity to silkworms and application thereof

The invention discloses a Beauveria bassiana strain having high pathogenicity to silkworms and an application of the Beauveria bassiana strain in the production of Bombyx Batryticatus. The strain is preserved at the China General Microbiological Culture Collection Center on February 24, 2014 and the preservation number is CGMCC NO. 8861. The method for producing Bombyx Batryticatus by using the strain disclosed by the invention comprises the steps of preparing a spore suspension by using Beauveria bassiana and sterile water containing 0.2% of Tween-80, uniformly spraying the spore suspension onto the fifth-instar silkworm and inoculating for 4-5 days and obtaining Bombyx Batryticatus after a large quantity of silkworm died from muscardine. The Beauveria bassiana strain disclosed by the invention has high pathogenicity to the silkworm, the death time of the silkworms is consistent, the rigescence rate is high and is conducive to solving the problems about the standard production, quality control of Bombyx Batryticatus and the like.
Owner:SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI

Improved low energy adaptive clustering hierarchy (LEACH) method

The invention discloses an improved low energy adaptive clustering hierarchy (LEACH) an method. The method comprises the following steps of according to a node position, distributing an Area_ID number, combining residual node energy to carry out cluster head selection and realizing partition clustering; taking a third-order polynomial logarithm distance path loss model as an energy consumption model and ensuring practicality of a simulation result; and according to the Area_ID number of the node, using TD-FDMA to select different channels to carry out data transmission, and determining reliability of data transmission. Compared to an existing LEACH, by using the method in the invention, node death time is concentrated; energy losses of a network node can be balanced; monitoring blind spot appearance time is short; a network life cycle is prolonged and a sensor node is economic and high-efficient. The improved LEACH route algorithm is provided and the route algorithm with high energy efficiency, good robustness and a characteristic approaching intelligent-household practical application is realized.
Owner:HUNAN UNIV OF TECH

Production method of white muscardine silkworms

The invention discloses a production method of white muscardine silkworms. The production method comprises the following steps of: (1) rejuvenescence of pathogens: adopting white muscardine silkworms to infect bombyx mori polypides and collecting fresh beauveria bassiana on the bombyx mori polypides; (2) toxin attacking for the pathogens: preparing the beauveria bassiana into beauveria bassiana liquid, closing doors and windows, uniformly spraying the beauveria bassiana liquid on silkworm bodies with newly-exuviated fifth larva and placing in a sealed space for 1-2 hours; (3) culture of silkworm bodies: opening doors and windows for ventilation, controlling the temperature to 22-26 DEG C and the humidity to 95-98%, and culturing till the silkworm bodies are died; (4) culture of muscardine silkworms: choosing out died silkworm bodies, culturing on a clean silkworm seat paper, controlling the temperature to 24-28 DEG C and the humidity to more than or equal to 80%, culturing till rigidification of the died silkworm bodies, growing white hypha conidia and obtaining semi-finished products of the muscardine silkworms; and (5) preparation of finished products of the white muscardine silkworms: collecting semi-finished products of the white muscardine silkworms, airing and drying to obtain the finished products of the white muscardine silkworms. The production method disclosed by the invention has the advantages that the death time of the silkworm bodies is neat, the rigidification time is short, the rigidification rate is high, the yield of the white muscardine silkworms is high and the quality is stable.
Owner:SICHUAN ACAD OF AGRI SCI SERICULTURE INST +1

Fish protector

The invention relates to a fish protector, in particular to an oxygen supply fish protector which comprises a box, a carbon dioxide filter, an air inlet pipe, an air outlet pipe, an oxygen content sensor, a central processor and an air pump. The air inlet pipe is communicated with an air inlet of the air pump, the carbon dioxide filter is arranged on the air inlet pipe, the air outlet pipe is arranged in the box, a plurality of air outlet holes are formed in the wall of the air outlet pipe, an air inlet of the air outlet pipe is communicated with an air outlet of the air pump, gas pumped from the air outlet of the air pump is exhausted into the box through the air outlet holes, a sensing end of the oxygen content sensor is arranged in water inside the box, and the air pump and the oxygen content sensor are respectively electrically connected with the central processor. The fish protector automatically supplies oxygen for the water in the fish protector to solve the problems of short delayed fish death time, difficulty in fixing, potential safety hazards and short service life of an existing fish protector.
Owner:怀宁县鲶鱼湾生态农业有限公司

Method of wireless sensor network clustering by using Hopfield nerve network

Disclosed is a method of wireless sensor network clustering by using Hopfield nerve network. The method belongs to the technical field of wireless sensor network, improves deficiencies of LEACH (Low Energy Adaptive Clustering Hierarchy) protocol to balance energy consumption of each node and distribution of each cluster-head, and models the clustering protocol into a combination optimization problem. The method improves traditional Hopfield nerve network local extreme value problem and fixed step length problem, and provides a dynamic step length chaotic Hopfield nerve network under the name of DSC-HNN. DSC-HNN achieves best cluster-head selection. The method can effectively achieve the principle of protocol, delay the death time of nodes to maximize the life cycle of the wireless sensor network to prolong the life cycle of network.
Owner:SHANDONG UNIV

Cross-layer routing method utilized in cognitive radio cellular network and based on energy optimization and network lifetime

ActiveCN103179632AMake optimal joint allocations in real timeAvoid premature depletion of energyPower managementHigh level techniquesCognitive userFrequency spectrum
The invention discloses a cross-layer routing method utilized in a cognitive radio cellular network and based on energy optimization and network lifetime, and relates to the cognitive radio field in wireless communication technology. The cross-layer routing method has the advantages that new routing metrics are provided specific to characteristics that various cognitive users in the cognitive radio cellular networks have different dynamic spectrum resources and energy of wireless cognitive nodes is limited, route selection and channel assignment in the cognitive network are considered in a combined manner by the aid of a cross-layer technology, a priority algorithm for the shortest path is modified, the problem that load on individual nodes is excessively high, energy is used up in advance and the individual nodes fail are solved, death time of each node is delayed, the lifetime of the network is prolonged, large quantities of relay nodes can be selected as cognitive nodes owing to large quantities of available effective nodes, accordingly, the average energy consumption is reduced integrally, and a communication access success rate is increased.
Owner:COMM ENG COLLEGE SCI & ENGINEEIRNG UNIV PLA

Use of compound Chinese lobelia preparation for preparing medicament for treating tumor

The invention discloses a new purpose of a compound Chinese Lobelia formulation for treating tumor or treating tumor in conjunction with chemotherapeutic treatment and radiation treatment, which belongs to the field of traditional Chinese medicine. Aiming at various injuries such as universal and serious damage to immunity of the organism, obvious reduction of white blood cells, bacterial infection, pain, and the like caused by simulative chemotherapeutic treatment and radiation treatment to a patient who suffers from tumor, experimental results indicate that the compound Chinese Lobelia formulation can obviously enhance the immunologic function, can remarkably increase the phagocytic function of scavenger cells, has protective action for reducing white blood cells, and has the weighting function for spleen and thymus; the compound Chinese Lobelia formulation has a certain in-vitro antibacterial action for staphylococcus aureus (G+) and colon bacillus (G-), has obvious protective function for a mouse infected by staphylococcus aureus and colon bacillus, can obviously reduce the death rate of the infected mouse and delay the death time to some extent; and the compound Chinese Lobelia formulation has obvious analgestic action for rats and mousse. The experimental results explain that the compound Chinese Lobelia formulation can be used for treating tumor or used for treating tumor in conjunction with chemotherapeutic treatment and radiation treatment and can increase the tolerance and the adaptability of a patient for chemotherapeutic treatment, strengthen the immunity of an organism, shorten the period of chemotherapeutic treatment or radiation treatment, reduce the toxic side reaction in chemotherapeutic treatment or radiation treatment, lower the death rate of a tumor patient and improve the life quality of a patient.
Owner:FUREN PHARMA GROUP

Chicken Newcastle disease virus and its separation method

The invention relates to the field of animal virology and provides a chicken Newcastle disease virus and its separation method. The virus is code-named SDM01 and the invention provides its separation and preparation method. The inventor carries out biological collection for the strain and its collection number is CCTCC NO:V201109. Through chicken embryo passage, biological characteristics tests such as a hemagglutinin and serum neutralization test, an animal regress test and exogenous virus testing, are carried out. The test results confirm that the virus is the Newcastle disease virus. It isproved through chicken embryo mean death time (MDT), one-day-old chick intracebral pathogenicity index (ICPI) measurement and 6-week-old chick intravenous inoculation pathogenicity index (IVPI) measurement that the Newcastle disease virus is a virulent strain. Positive serum is prepared through separated strain and standard strain to carry out an HI cross neutralization test, a chicken embryo neutralization test, a cell neutralization test, an F and HN gene sequence sequencing and comparison and an immunity protective test. Results confirm that there exist large differences between the Newcastle disease virus SDM01 strain and traditional strains (La Sota strain and Clone30 strain) in both genetic typing and antigenicity.
Owner:秦卓明 +1

Method for testing combined pollution toxicity of chlorpyrifos and butachlor

PendingCN109952980AIncreased environmental riskFacilitate the true reflection of objective requirementsData processing applicationsBiological material analysisChlorpyrifosCvd risk
The invention discloses a method for testing the combine pollution toxicity of chlorpyrifos and butachlor. The method comprises the step that A, experimental organisms are prepared, specifically, a zebra fish wild type AB strain is adopted in an experiment and is domesticated in a laboratory after being purchased, and adult fish for collecting fish eggs for the experiment has been bred for more than one month in the laboratory. According to the method, a combined toxicity test and a single pesticide test are combined, so that reference data are conveniently increased according to the influenceof different environments and different reagents on animals, the cross reference of the data is improved, the influence of irrelevant variables is well balanced and counteracted, the experimental result is more persuasive, the toxicity of pesticides on fish fries is calculated by utilizing a probability value analysis method according to the mortality and the death time of the fish fries, reasonable analysis and treatment are carried out on the data, the phenomenon that related evaluation standard for pollution set by dependence on a single effect can be avoided, the objective requirements ofthe real environment quality can be conveniently and truly reflected, and therefore the environmental risk of pollutants in environmental water can be effectively evaluated, and the environmental threshold value which is safer to aquatic organisms can be formulated conveniently.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Method for testing low-temperature-resistant character of Litopenaeus vannamei

The invention provides a method for testing the low-temperature-resistant character of Litopenaeus vannamei. The method comprises the following steps: 1) slow cooling for temporary rearing, 2) low-temperature stress treatment and 3) data and sample collection, wherein cooling rate in step 1) is 1 DEG C / d; cooling rate in step 2) is 2 DEG C / d; families and individuals with good low-temperature-resistant character are determined according to low-temperature accumulated survival time and temperature at death time. One novel method is provided for improving low-temperature adaptability of prawns,with adoption of the method, low-temperature tolerance of the Litopenaeus vannamei can be improved remarkably, energy cost for maintaining high temperature in a breeding process is reduced, and breeding economic benefit is increased.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Oat hybridization technology and hybridization stalk using technology

The invention relates to an oat hybridization technology and a hybridization stalk using the technology. The oat hybridization technology and the design of the hybridization stalk using the technologycan achieve the effect of fixing and protecting mathernal plants; the risk of mathernal plant press bending or fracture under the rainy or windy climate conditions due to self weight of a cloth bag and great surface area can be effectively avoided. The father plant withering and death time can be effectively delayed; the anther survival and pollination time can be further prolonged. The father plant pollen release time and the mathernal plant pollination rate can be effectively increased. The interference of mathernal plants by other pollen can be effectively avoided; the damage of bad conditions such as hailstone and heavy rain on hybridization mathernal flower organs can be avoided; the seed falling is prevented and controlled in the seed ripening period; the smooth growth of the hybridization seeds can be protected.
Owner:YANGZHOU UNIV

Method for fixing and extracting biological tissue by using immobile liquid to deduce death time of biological tissues

The invention relates to a method for fixing and extracting a biological tissue by using immobile liquid to deduce the death time of the biological tissue. By adopting the method, kinds of detection methods are applied to analyze on the basis of the principle that immobile liquid can quickly terminate self-digestion and decay change processes of an in vitro biological tissue, so as to extract a material composition in the biological tissue and achieve dynamic balance within a certain period of time; finally, the postmortem interval (PMI) is indirectly deduced according to the scheduling rule of occurrence and development of self-digestion and decay of the biological tissue. The method comprises the steps: sampling 'six-fixing' method, drafting of a 'window phase' standard curve, and deducing of the PMI. By adopting the method disclosed by the invention, the in vitro biological tissue can be effectively prevented from continuing to generate self-digestion and decay between the processes of drawing materials and detecting by the sampling 'six-fixing' method, so that the accuracy and the scientificity of deducing the PMI are improved.
Owner:SHANTOU UNIV MEDICAL COLLEGE

Medicine for treating epilepsia and preparation method

The invention relates to the field of treatment of epilepsia and discloses a medicine for treating epilepsia and a preparation method. The medicine is prepared from the following components in parts by weight: 10 to 30 parts of radix gentianae extracts, 10 to 20 parts of radix scutellariae water extracts, 5 to 15 parts of radix salviae miltiorrhizae alcohol extracts, 20 to 40 parts of radix angelicae sinensis alcohol extracts, and 5 to 15 parts of radix ophiopogonis water extracts. The radix angelicae sinensis alcohol extracts of the medicine disclosed by the invention has a convulsion symptomcaused by reducing pentylenetetrazole, has treatment effects on reduction of convulsion death ratios, delay of death time, prolong of convulsion latent time and the like, and also has the treatment effects on resisting convulsion and epilepsia.
Owner:成都图瑞思舟科技有限公司

Method for obtaining postmortem interval of mouse

The invention relates to a method for obtaining the postmortem interval of a mouse. The method comprises the steps of (1) collecting mouse samples, performing extraction to obtain DNA, and performing metagenome analysis on sample oral microorganisms through high-flux sequencing; and (2) according to the structural composition of oral microorganism communities and succession law occurring along with death time increase, obtaining species of which the relative abundance has dependency with death time, and establishing a linear regression model of the postmortem interval and the relative abundance of the species. The method disclosed by the invention is convenient to sample, the influence of external environment and subjective judgment of an inspector is reduced, and increase of death time inference precision is facilitated.
Owner:DONGHUA UNIV +1

Method of using silver nanocluster to detect potassium ion concentration in vitreous fluid to deduce death time

The invention belongs to the technical field of medicine, and particularly relates to a method of using silver nanocluster to detect potassium ion concentration in vitreous fluid to deduce death time. The method includes that a novel DNA strand containing potassium ion aptamer is used as a template to synthesize DNA-AgNCs having fluorescent property, the system specifically recognizes K+ to quench fluorescence of the silver nanocluster, and a standard curve is drawn according to changing of the fluorescence; vitreous fluid to be detected is added into a DNA-AgNCs solution, potassium ions in the vitreous fluid lead to changing of the fluorescence of the silver nanocluster, the potassium ion concentration in the detected vitreous fluid is acquired by comparing with the standard curve; a standard curve between K+ concentration in a vitreous fluid sample and known death time is drawn, and a result has high uniformity to reported death time and vitreous fluid potassium ion relation. The method can be used for deducing death time by legal medical experts, has high specificity and sensitivity, is simple and quick to operate and low in cost and can be applied to detecting potassium ion content in other body fluids and water environment.
Owner:CENT SOUTH UNIV

Control method and system of single photon detector death time

The invention discloses a method for controlling the death time of a single-photon detector, which is characterized in that it comprises the following steps: obtaining a single-photon detection signal; rewriting a register and obtaining a corresponding resistance value; the resistance value and the capacitance jointly determine the death time According to the invention, the value of the dead time of the single photon detector in the quantum key distribution system can be adjusted conveniently, and the stability is better.
Owner:浙江科易理想量子信息技术有限公司

A method for revising big data genealogy

A method for revising big data genealogy is disclosed. The method includes the following steps: firstly, establishment of a character data the character data includes number, name information, sex information, birth and death information, other name information and introduction information, the birth and death information includes birth information and death information, the birth informationincludes birth time and birth place, and the death information include death time and death place; secondly, data searching: including character searching and character relationship searching; thirdly, genealogy revision: linking the data of direct kinship, dividing the generations and perfecting the genealogy. This method of family tree generation based on big data adopts decentralized terminalsto upload branch line genealogy and aggregate it into a large database. Decentralized feeder genealogy is combined and connected by comparing data from character and relationship searches. The more feeder genealogies are entered, the more complete and complete the merged and linked genealogies become.
Owner:GUIZHOU QIANSHANG TECH CO LTD

Corpse temperature real-time measurement recording analyzer and death time analysis method

ActiveCN112113683AComputing scientifically accurateFast automatic calculationThermometer applicationsComplex mathematical operationsAmbient humidityProcess engineering
The invention provides a corpse temperature real-time measurement recording analyzer and a death time analysis method. The corpse temperature real-time measurement recording analyzer comprises a hostand at least one temperature probe, the host integrates a processing unit, a storage unit, a display screen and an environment temperature and humidity sensor. A temperature probe is in a round bar shape and is connected to a first signal acquisition end of the processing unit through a signal line. The output end of the environment temperature and humidity sensor is connected to a second signal acquisition end of the processing unit. The display screen is used for displaying the current environment temperature T1, the environment humidity, the corpse temperature T2 obtained by the current temperature probe and the calculated death time X. Compared with the prior art, the method has the advantages that the corpse temperature, the environment temperature and other influence factors are integrated, and the reliable death time is quickly and automatically calculated.
Owner:SHAANXI PROVINCIAL PUBLIC SECURITY DEPT

Electronic meter type electronic thermometer

The invention provides an electronic meter type electronic thermometer. The electronic meter type electronic thermometer can be worn on one hand of a user, is capable of carrying out real-time monitoring on the temperature of the user through a body temperature monitoring circuit, giving out a body temperature abnormal alarm when the body temperature of the user is abnormal, and is also capable of giving out a death alarm and automatically recording the death time when the body temperature of the user is lower than the life survival temperature, namely, the user is dead. The electronic meter type electronic thermometer is characterized by comprising a real-time body temperature monitoring circuit, a liquid crystal display, an electronic meter type shell and an elastic watchband. The real-time body temperature monitoring circuit is used for monitoring the body temperature of the user in real time, giving out the body temperature abnormal alarm when the body temperature of the user is abnormal, and giving out the death alarm and automatically recording the death time when the body temperature of the user is lower than the life survival temperature, namely, the user is dead. The liquid crystal display is used for displaying the time, the date, the body temperature of the user, working conditions and other information. The elastic watchband is capable of enabling the electronic meter type shell to be closely attached to the hand of the user.
Owner:刘康

A family tree generation method based on big data

The invention discloses a family tree generation method based on big data, the method comprising steps: 1, establishing a character data the character data includes numbers, name information, sexinformation, birth and death information, other name information and introduction information, wherein the birth and death information includes birth information and death information, the birth information includes birth time and birth place, and the death information include death time and death place; 2, establishing a genealogy database, wherein that data of the person connected with the direct kinship are input into the database according to the last name, and the genealogy is established accord to the generations of the clans; 3, genealogy generation: that genealogy is derived accordingto the genealogy data and the genealogy algebra. The method of family tree generation based on big data adopts decentralized terminals to upload branch line genealogy and aggregate it into a large database. Combining and linking decentralized feeder genealogy by comparing data from character and relationship searches is performed. The more feeder genealogies are entered, the more complete and complete the merged and connected genealogies are, and then the genealogies are generated according to the required algebra.
Owner:GUIZHOU QIANSHANG TECH CO LTD

Cross-layer Routing Method Based on Energy Optimization and Network Lifetime in Cognitive Radio Cellular Networks

The invention discloses a cross-layer routing method utilized in a cognitive radio cellular network and based on energy optimization and network lifetime, and relates to the cognitive radio field in wireless communication technology. The cross-layer routing method has the advantages that new routing metrics are provided specific to characteristics that various cognitive users in the cognitive radio cellular networks have different dynamic spectrum resources and energy of wireless cognitive nodes is limited, route selection and channel assignment in the cognitive network are considered in a combined manner by the aid of a cross-layer technology, a priority algorithm for the shortest path is modified, the problem that load on individual nodes is excessively high, energy is used up in advance and the individual nodes fail are solved, death time of each node is delayed, the lifetime of the network is prolonged, large quantities of relay nodes can be selected as cognitive nodes owing to large quantities of available effective nodes, accordingly, the average energy consumption is reduced integrally, and a communication access success rate is increased.
Owner:COMM ENG COLLEGE SCI & ENGINEEIRNG UNIV PLA

Production method of whole-piece instant fish meat

The invention discloses a production method of whole-piece instant fish meat. The production method comprises the following steps that 1) fish of which the death time is that very day are selected, and the quality of selected fish meat is ensured; 2) external impurities and internal impurities of the fish are removed, secondary cleaning is carried out, the fish meat is cut into slices with a knife, and the thickness of the fish slices does not exceed 5 mm; 3) the fish slices are pickled; 4) drying and dewatering are carried out; 5) drying and cooling are carried out; 6) the cooled cooked fishslices are subpackaged into polyethylene film small bags for vacuum package. The production method of the whole-piece instant fish meat has the advantages that the design is reasonable, the steps aresimple, the operability is strong, the nutritive value and delicious taste of fish cannot be destroyed, different mouthfeels can also be produced, the rich mouthfeels increase the selection of people,it is ensured that the whole-piece instant fish meat cannot contain high-content grease, and the health of consumers is protected.
Owner:青岛一鲁鲜食品有限公司

Application of astragalus polysaccharide on drosophila melanogaster in oxidation resistance and aging resistance

The invention discloses application of astragalus polysaccharide on drosophila melanogaster in oxidation resistance and aging resistance, improvement of oxidation resistance of the drosophila melanogaster, enhancement of constitution of the drosophila melanogaster and improvement of improvement aging resistance of the drosophila melanogaster. By feeding of drosophila melanogaster culture media atdifferent concentration, the change of the astragalus polysaccharide for life and antioxidant enzyme activity of the drosophila melanogaster is studied in order to provide certain experimental basis for development of astragalus polysaccharide. When the concentration of the astragalus polysaccharide is 1.0 [mu] g / mL, the average life, half death time and the highest life of male and female drosophila melanogaster can be prolonged, the inverse-gravity crawling capacity of the drosophila melanogaster can be enhanced, the SOD enzyme activity and T-AOC content of organisms are the highest, and theMDA content is the lowest.
Owner:长治学院

Preparation method of compound inactivated vaccine for treating avian influenza

The invention provides a preparation method of a compound inactivated vaccine for treating avian influenza, belonging to the technical field of biological products for livestock. The preparation method comprises the following process steps: (1) preparing an inactivated virus suspension; and (2) preparing the compound inactivated vaccine for treating avian influenza. After a dilute solution prepared by the preparation method is inoculated to a chick embryo, the virus resistance of the chick embryo can be increased, and the death time can be prolonged, namely that the virus proliferation time can be prolonged; and compared with the prior art, the virus titer is increased by more than 8 times. According to the invention, a new vaccine adjuvant is adopted; and the prepared vaccine has double characteristics of water in oil and oil in water and integrates the advantages of the slow release effect of the vaccine and the combined action of water-soluble immune enhancers, egg yolk antibodies and the like. According to the invention, because effective immune enhancers are adopted, the immunity of an organism is greatly increased, the humoral immunity is increased, the organism is stimulated to generate an efficient neutralizing antibody, and the disease prevention capability of the organism is increased.
Owner:浙江美保龙生物技术有限公司

Chicken Newcastle disease virus and its separation method

The invention relates to the field of animal virology and provides a chicken Newcastle disease virus and its separation method. The virus is code-named SDM01 and the invention provides its separation and preparation method. The inventor carries out biological collection for the strain and its collection number is CCTCC NO:V201109. Through chicken embryo passage, biological characteristics tests such as a hemagglutinin and serum neutralization test, an animal regress test and exogenous virus testing, are carried out. The test results confirm that the virus is the Newcastle disease virus. It isproved through chicken embryo mean death time (MDT), one-day-old chick intracebral pathogenicity index (ICPI) measurement and 6-week-old chick intravenous inoculation pathogenicity index (IVPI) measurement that the Newcastle disease virus is a virulent strain. Positive serum is prepared through separated strain and standard strain to carry out an HI cross neutralization test, a chicken embryo neutralization test, a cell neutralization test, an F and HN gene sequence sequencing and comparison and an immunity protective test. Results confirm that there exist large differences between the Newcastle disease virus SDM01 strain and traditional strains (La Sota strain and Clone30 strain) in both genetic typing and antigenicity.
Owner:秦卓明 +1

Chicken infectious bronchitis virus diluent and preparation method thereof

InactiveCN105368790ALow degree of differentiationEasy to proliferateViruses/bacteriophagesPhosphateTryptophan
The invention relates to a chicken infectious bronchitis virus diluent and a preparation method thereof and belongs to the technical field of animal biological products. Every 1000 ml of phosphate buffer in the diluent comprises, by weight, 1-4 g of amino acid combination, 1-5 g of vitamin combination, 12-18 mg of sodium selenite and 5-25 g of compound traditional Chinese medicine polysaccharide. The amino acid combination comprises methionine, tryptophan, tyrosine and leucine. The vitamin combination comprises vitamin B2, vitamin D and vitamin E. The compound traditional Chinese medicine polysaccharide is obtained by extraction from curculigo orchioides, eucommia ulmoides, poria cocos and dipsacus asperoids. After chick embryos are inoculated with the diluent, the virus resistance of the chick embryos can be improved, the death time is prolonged, and the virus multiplication time is prolonged. Compared with the prior art, the virus titer is improved by twice to eight times. According to the prepared diluent, the low-day-old chick embryos are inoculated, it is incidentally found that the size of obtained allantoic fluid is greatly increased, and the size of the obtained allantoic fluid is increased by 30-40%.
Owner:浙江美保龙生物技术有限公司

Freezing method of goat epididymal sperms

The invention discloses a freezing method of goat epididymal sperms. The method includes the following steps of firstly, separating testicles with scrotum from animal bodies to be stored; secondly, separating epididymis from the stored testicles with scrotum; thirdly, incubating sperms in the epididymis separated out; fourthly, processing the incubated sperms through a diluent and a mixed solution; fifthly, freeze-preserving the processed sperms at -250-0 DEG C, wherein the animal death time is 0-72 h and preferentially 0-24 h. Through the research on the freezing effect of the goat epididymalsperms, the method capable of successfully freeze-preserving the goat epididymal sperms is developed.
Owner:柴局
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