Preparation method of compound inactivated vaccine for treating avian influenza

A technology of inactivated vaccine and avian influenza, which is applied in the field of preparation of compound inactivated vaccine for avian influenza, can solve the problems of wasting manpower and material resources, poultry stress response, and productivity decline, so as to improve immunity, prolong death time, and improve tolerance. Ability to be affected by viruses

Inactive Publication Date: 2016-05-04
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, my country's preventive measures against poultry diseases are still based on vaccination, supplemented by other methods, but the vaccines used are dominated by conventional vaccines, including inactivated vaccines and attenuated or non-toxic live vaccines, and most of them are single vaccines. Multiple immunizations are required in the prescribed immunization procedures, which not only wastes manpower and material resources, but also easily causes stress reactions in poultry, resulting in a decrease in productivity and disease resistance.

Method used

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  • Preparation method of compound inactivated vaccine for treating avian influenza
  • Preparation method of compound inactivated vaccine for treating avian influenza
  • Preparation method of compound inactivated vaccine for treating avian influenza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] An avian influenza virus dilution, which contains per 1000ml of phosphate buffer: 6g of amino acid combination (0.6g of glycine, 1.8g of nicotinamide, 2.4g of leucine, 1.2g of ascorbic acid), 15g of compound Chinese medicine flavonoids, lipid Body 15g, the pH of the diluent is 6.9.

[0041] A kind of preparation method of avian influenza virus dilution, its step is:

[0042] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic conditions, and filter and sterilize for later use.

[0043] (2) Preparation of compound traditional Chinese medicine flavonoids

[0044] a. Weigh the raw materi...

Embodiment 2

[0053] An avian influenza virus dilution, which contains per 1000ml of phosphate buffer: 8g of amino acid combination (1.6g of glycine, 1.6g of nicotinamide, 2.4g of leucine, 2.4g of ascorbic acid), 18g of compound Chinese medicine flavonoids (from 20 parts, 15 parts of Sophora flavescens, 10 parts of white bark of Toonia, 25 parts of Baiwei, 15 parts of Gentiana and 15 parts of Prunella vulgaris), liposome 20g (prepared according to Example 1), the diluent The pH is 7.2.

[0054] A kind of preparation method of avian influenza virus dilution, its step is the same as embodiment 1.

Embodiment 3

[0056] A dilution of avian influenza virus, the virus dilution contains per 1000ml of phosphate buffer: 10g of amino acid combination (2.5g of glycine, 3.0g of nicotinamide, 3.0g of leucine, 1.5g of ascorbic acid), 5g of compound Chinese medicine flavonoids (from Coptis chinensis 35 parts, 5 parts of Sophora flavescens, 15 parts of Cortex Tooniae, 10 parts of Bai Wei, 20 parts of Gentiana and 15 parts of Prunella vulgaris), liposome 10g (prepared according to Example 1), the diluent The pH is 7.5.

[0057] A kind of preparation method of avian influenza virus dilution, its step is the same as embodiment 1.

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Abstract

The invention provides a preparation method of a compound inactivated vaccine for treating avian influenza, belonging to the technical field of biological products for livestock. The preparation method comprises the following process steps: (1) preparing an inactivated virus suspension; and (2) preparing the compound inactivated vaccine for treating avian influenza. After a dilute solution prepared by the preparation method is inoculated to a chick embryo, the virus resistance of the chick embryo can be increased, and the death time can be prolonged, namely that the virus proliferation time can be prolonged; and compared with the prior art, the virus titer is increased by more than 8 times. According to the invention, a new vaccine adjuvant is adopted; and the prepared vaccine has double characteristics of water in oil and oil in water and integrates the advantages of the slow release effect of the vaccine and the combined action of water-soluble immune enhancers, egg yolk antibodies and the like. According to the invention, because effective immune enhancers are adopted, the immunity of an organism is greatly increased, the humoral immunity is increased, the organism is stimulated to generate an efficient neutralizing antibody, and the disease prevention capability of the organism is increased.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a preparation method of a bird flu compound inactivated vaccine. Background technique [0002] Avian influenza virus (Avian influenza virus, AIV) belongs to Orthomyxoviridae, Influenza virus, type A influenza virus. After AIV infects poultry, it can cause an infectious disease characterized by respiratory system and even systemic sepsis, that is, avian influenza (Avian influenza, AI). AI can not only spread among poultry, seriously affecting the survival and development of animal husbandry; it can also be transmitted from poultry to humans, directly threatening human health and causing great harm to humans. In the 20th century, four worldwide influenza pandemics broke out in human history, among which the "Spanish flu" that broke out in 1918 was the most harmful to human society. According to incomplete statistics, the worldwide influenza pand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61K39/145A61K39/39A61P31/16A61K36/718A61K36/489A61K36/58A61K36/27A61K36/515A61K36/536A61K38/06
CPCA61K39/12A61K36/27A61K36/489A61K36/515A61K36/536A61K36/58A61K36/718A61K38/06A61K39/39A61K39/395A61K2236/331C12N2760/16134
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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