Newcastle disease virus diluent and preparation method thereof
A technology for chicken Newcastle disease virus and diluent, which is applied to the field of chicken Newcastle disease virus diluent and its preparation, can solve the problems such as lowering of the poison price and low content of allantoic fluid, and achieves an increase in the volume of the allantoic fluid, the ability to tolerate the virus, and the prolongation of the The effect of time of death
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Embodiment 1
[0036] A chicken Newcastle disease virus dilution, the virus dilution contains per 1000ml of phosphate buffer: amino acid combination 8g (wherein phenylalanine 3.2g, cystine 2.4g, leucine 2.4g), vitamin combination 3g (wherein vitamin B20.9g, vitamin D0.9g, vitamin E1.2g), compound Chinese medicine polysaccharide 15g, sodium selenite 16mg, the pH of the diluent is 6.8.
[0037] A kind of preparation method of chicken Newcastle disease virus dilution, its steps are:
[0038] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic conditions, and filter and sterilize for later use.
[0039] (2) Pre...
Embodiment 2
[0048] A chicken Newcastle disease virus dilution, this virus dilution is every 1000ml phosphate buffer (this phosphate buffer is made of sodium chloride 6g, potassium chloride 0.05g, disodium hydrogen phosphate 1g, potassium dihydrogen phosphate 0.05g, calcium chloride 0.05 g, 0.05g magnesium chloride containing 6 crystal waters dissolved in 1000ml double distilled water) contains: amino acid combination 6g (including phenylalanine 3g, cystine 1.2g, leucine 1.8g), vitamin combination 5g ( Among them, vitamin B22g, vitamin D1g, vitamin E2g), compound Chinese medicine polysaccharide 20g (extracted from 20 parts of red peony root, 5 parts of salvia miltiorrhiza, 10 parts of Bai Wei, 5 parts of pokeweed and 10 parts of Cordyceps), 12 mg of sodium selenite, The pH of this dilution is 6.9.
[0049] A kind of preparation method of chicken Newcastle disease virus dilution, its step is the same as embodiment 1.
Embodiment 3
[0051] A chicken Newcastle disease virus dilution, this virus dilution is every 1000ml phosphate buffer (this phosphate buffer is made of sodium chloride 10g, potassium chloride 0.5g, disodium hydrogen phosphate 1.2g, potassium dihydrogen phosphate 0.5g, calcium chloride 0.2g, 0.2g of magnesium chloride containing 6 crystal water dissolved in 1000ml of double distilled water) contains: amino acid combination 10g (including phenylalanine 1.5g, cystine 0.6g, leucine 0.9g), vitamin combination 1g (24g of vitamin B, 2g of vitamin D, 4g of vitamin E), 5g of compound Chinese herbal polysaccharides (extracted from 40 parts of red peony root, 20 parts of salvia miltiorrhiza, 30 parts of Bai Wei, 20 parts of pokeweed and 30 parts of cordyceps), sodium selenite 18mg, the pH of this dilution is 7.
[0052] A kind of preparation method of chicken Newcastle disease virus dilution, its step is the same as embodiment 1.
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