Newcastle disease virus diluent and preparation method thereof

A technology for chicken Newcastle disease virus and diluent, which is applied to the field of chicken Newcastle disease virus diluent and its preparation, can solve the problems such as lowering of the poison price and low content of allantoic fluid, and achieves an increase in the volume of the allantoic fluid, the ability to tolerate the virus, and the prolongation of the The effect of time of death

Inactive Publication Date: 2016-03-02
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, 10-day-old chicken embryos are used for Newcastle disease virus inoculation and breeding. Generally, chicken embryo fluid is harvested after 96-120 hours of cultivation, that is, 14-15 days old. At this time, the age is too old and the allantoic fluid content is not high (chicken embryo The allantoic fluid content is the highest at the age of 13 to 14 days of development); if the allantoic fluid content is increased, it must be harvested in advance, but the virus content has not reached the peak at this time, and the reduction of the poison price is not worth the loss.

Method used

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  • Newcastle disease virus diluent and preparation method thereof
  • Newcastle disease virus diluent and preparation method thereof
  • Newcastle disease virus diluent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A chicken Newcastle disease virus dilution, the virus dilution contains per 1000ml of phosphate buffer: amino acid combination 8g (wherein phenylalanine 3.2g, cystine 2.4g, leucine 2.4g), vitamin combination 3g (wherein vitamin B20.9g, vitamin D0.9g, vitamin E1.2g), compound Chinese medicine polysaccharide 15g, sodium selenite 16mg, the pH of the diluent is 6.8.

[0037] A kind of preparation method of chicken Newcastle disease virus dilution, its steps are:

[0038] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic conditions, and filter and sterilize for later use.

[0039] (2) Pre...

Embodiment 2

[0048] A chicken Newcastle disease virus dilution, this virus dilution is every 1000ml phosphate buffer (this phosphate buffer is made of sodium chloride 6g, potassium chloride 0.05g, disodium hydrogen phosphate 1g, potassium dihydrogen phosphate 0.05g, calcium chloride 0.05 g, 0.05g magnesium chloride containing 6 crystal waters dissolved in 1000ml double distilled water) contains: amino acid combination 6g (including phenylalanine 3g, cystine 1.2g, leucine 1.8g), vitamin combination 5g ( Among them, vitamin B22g, vitamin D1g, vitamin E2g), compound Chinese medicine polysaccharide 20g (extracted from 20 parts of red peony root, 5 parts of salvia miltiorrhiza, 10 parts of Bai Wei, 5 parts of pokeweed and 10 parts of Cordyceps), 12 mg of sodium selenite, The pH of this dilution is 6.9.

[0049] A kind of preparation method of chicken Newcastle disease virus dilution, its step is the same as embodiment 1.

Embodiment 3

[0051] A chicken Newcastle disease virus dilution, this virus dilution is every 1000ml phosphate buffer (this phosphate buffer is made of sodium chloride 10g, potassium chloride 0.5g, disodium hydrogen phosphate 1.2g, potassium dihydrogen phosphate 0.5g, calcium chloride 0.2g, 0.2g of magnesium chloride containing 6 crystal water dissolved in 1000ml of double distilled water) contains: amino acid combination 10g (including phenylalanine 1.5g, cystine 0.6g, leucine 0.9g), vitamin combination 1g (24g of vitamin B, 2g of vitamin D, 4g of vitamin E), 5g of compound Chinese herbal polysaccharides (extracted from 40 parts of red peony root, 20 parts of salvia miltiorrhiza, 30 parts of Bai Wei, 20 parts of pokeweed and 30 parts of cordyceps), sodium selenite 18mg, the pH of this dilution is 7.

[0052] A kind of preparation method of chicken Newcastle disease virus dilution, its step is the same as embodiment 1.

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Abstract

The invention relates to a newcastle disease virus diluent and a preparation method thereof and belongs to the technical field of animal biological products. Every 1000 ml of phosphate buffer in the diluent comprises, by weight, 6-10 g of amino acid combination, 1-5 g of vitamin combination, 12-18 mg of sodium selenite and 5-20 g of compound traditional Chinese medicine polysaccharide. The amino acid combination comprises phenylalanine, cystine and leucine. The vitamin combination comprises vitamin B2, vitamin D and vitamin E. The compound traditional Chinese medicine polysaccharide is obtained by extraction from red peony root, salviae miltiorrhizae, cynanchum atratum, radix phytolaccae and cordyceps militaris. After chick embryos are inoculated with the diluent, the virus resistance of the chick embryos can be improved, the death time is prolonged, and the virus multiplication time is prolonged. Compared with the prior art, the virus titer is improved by eight times or above. According to the prepared diluent, the low-day-old chick embryos are inoculated, it is incidentally found that the size of obtained allantoic fluid is greatly increased, and compared with the prior art, the size of the obtained allantoic fluid is increased by 40-50%.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a chicken Newcastle disease virus dilution and a preparation method thereof. Background technique [0002] Newcastle disease (ND) is a severe viral infectious disease caused by Newcastle disease virus (NDV). It is one of the most serious poultry infectious diseases in the world today and the most harmful of all chicken diseases in my country It is classified as a class A infectious disease by the International Office of Epizootics (OIE). [0003] At present, there are three methods for virus culture: animal inoculation, tissue culture and chicken embryo culture. Among them, chicken embryo culture is one of the most commonly used methods in virus culture. Chicken embryos are developing organisms, and a variety of animal viruses can multiply and pass on in chicken embryos. The advantage of chicken embryos is that the tissue differentiation degre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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