2 results about "Tissue Differentiation" patented technology

The present invention relates to a preparation method of a composition for culturing lung organoids, a composition thereby, and an organoid culture method using the same. The present invention is capable of creating an environment that is more similar to an actual tissue than a conventional MATRIGEL®-based culture system, and in particular, exhibits an effect of facilitating tissue differentiation in lung organoid culture and effectively developing into a form that is similar to an actual tissue.

The invention relates to the technical field of gene engineering, in particular to a nucleotide sequence for improving the differentiation efficiency of European spruce calluses, an expression vector and application of the nucleotide sequence, the nucleotide sequence is a coding sequence of an AP2L2 gene, and the expression vector comprises double-stranded RNA composed of the nucleotide sequence and a reverse complementary sequence of the nucleotide sequence. The invention discloses the negative regulation effect of the AP2L2 gene in European spruce callus differentiation for the first time, and the industrial problem that the differentiation efficiency is effectively promoted to be reduced through a specific gene silencing technology is solved. The scheme is feasible in operation, remarkable in effect and suitable for large-scale breeding and production of improved forest varieties.