70 results about "Tissue Differentiation" patented technology

The invention discloses a stem cell tissue culture seedling raising technique. The technique comprises the steps of 1, taking a fresh plant, conducting sterilization on the surface and cutting the plant; 2, conducting tissue differentiation and reserving a cambium; 3, culturing the cambium on an isolation medium. According to the technique, existing methods are improved based on a traditional tissue culture technique, plant stem cells are taken as a target, induction, separation and explant culture are conducted, and a corresponding stem cell culture system is established. By the adoption of the plant stem cell culture technique, the plant culture technique is enriched, and a novel research direction and opportunity is provided for commercialized production of natural products and development of the plant biotechnology.

There is provided a simplified and inexpensive method for the in-vitro identification, isolation and culture of human vasculogenic progenitor cells. The method and the progenitor cells isolated thereby can be used for in-vitro vascular engineering, treatment of congenital and acquired vascular and hematological abnormalities, for evaluation and development of drugs affecting vasculo- and angiogenic processes, and for further investigation into tissue differentiation and development.

The present invention provides a bioreacto 1r for incubation of cell cultures, tissue biopsies, cell clusters, tissue-like structures, ‘prototissues’ or similar samples. The bioreactor is generally adapted for rotation for use in microgravity conditions and equipped with an incubation cavity having a small internal fluid volume, generally less than 1 ml. To avoid problems associated with small volume incubation, the bioreactor may include a humidity chamber or other means of avoiding dehydration as well as substantially fluid-tight closures for access ports that avoid introduction of air bubbles to the incubation cavity. The small-volume bioreactor permits long term maintenance of tissue differentiation states in cultures. Also provided are methods of incubating cells or tissues using the bioreactor including methods of creating molecular profiles of biological effects of chemical compositions on differentiated cell or tissue samples maintained in long term culture.

The present invention provides a bioreacto 1r for incubation of cell cultures, tissue biopsies, cell clusters, tissue-like structures,apted for rotation for use in microgravity conditions and equipped with an incubation cavity having a small internal fluid volume, generally less than 1 ml. To avoid problems associated with small volume incubation, the bioreactor may include a humidity chamber or other means of avoiding dehydration as well as substantially fluid-tight closures for access ports that avoid introduction of air bubbles to the incubation cavity. The small-volume bioreactor permits long term maintenance of tissue differentiation states in cultures. Also provided are methods of incubating cells or tissues using thebioreactor including methods of creating molecular profiles of biological effects of chemical compositions on differentiated cell or tissue samples maintained in long term culture.

Systems and methods for image classification include receiving imaging data of in-vivo or excised tissue of a patient during a surgical procedure. Local image features are extracted from the imaging data. A vocabulary histogram for the imaging data is computed based on the extracted local image features. A classification of the in-vivo or excised tissue of the patient in the imaging data is determined based on the vocabulary histogram using a trained classifier, which is trained based on a set of sample images with confirmed tissue types.

The invention discloses a silicosis treatment aerosol containing a human mesenchymal stem cell exosome extract and a preparation method thereof, wherein the main component of the aerosol is the high-concentration mesenchymal stem cell exosome extract. The mesenchymal stem cells can secrete various cell factors in the culture process, and the cell factors can penetrate into the basal layer of an inner membrane, promote tissue differentiation of the inner membrane, angiogenesis and growth of granulation tissues, promote regeneration and repair of damaged skin tissue structures, reduce generation of scar connective tissues and reduce bacterial infection and inflammatory reaction of wound surface tissues. According to the aerosol and the preparation method thereof, a method of continuous starvation of the mesenchymal stem cells is adopted, a high-concentration human mesenchymal extract can be obtained in a short period of time, and a big amount of stem cell exosomes can be separated from the mesenchymal extract by an exosome extractor. The aerosol containing the high-concentration mesenchymal stem cell exosome extract disclosed by the invention is suitable for the restoration and treatment of silicosis.

A simplified and inexpensive method for the in-vitro identification, isolation and culture of human vasculogenic progenitor cells is provided. The method and the progenitor cells provided herein can be used for in-vitro vascular engineering, treatment of congenital and acquired vascular and hematological abnormalities, for evaluation and development of drugs affecting vasculo- and angiogenic processes, and for further investigation into tissue differentiation and development.

The invention provides a fractured end micro-movement and blood supply based fracture healing simulation system, relating to the field of biomedical engineering. The system is used for predicting a complicated process of fracture healing and exploring undetermined mechanisms in fracture healing. The system comprises a fracture healing simulation sub-system, an input module and a display module. The fracture healing simulation sub-system (1) comprises a geometry modeling module, a grid partition module, an environment initialization module, a biomechanical calculation module and a biological environment module. The biological environment module is used for creating a biochemical environment of callus in fracture healing so as to finish tissue differentiation in the callus. According to the system, blood supply is used as a dynamic variable and is introduced to the fracture healing simulation system, so that the fracture healing process can be simulated more accurately. The system can perform experiment simulation repeatedly, thereby helping to find out the best fractured end micro-movement value and the impact of blood on fracture healing.

A method of differentiating tissues in Magnetic Resonance Imaging (MRI) comprising applying a Magnetization Transfer (MT) pre-pulse in combination with the Diffusion Weighted Imaging (DWI) pulse sequence to obtain an image of the tissue under evaluation. Analysis maps and/or measurements are generated from the obtained image, from which values representative of the macromolecular content are computed for obtaining tissue differentiation.

The invention relates to a conjoined grafting method for plant meristem. The conjoined grafting method for the plant meristem is characterized in that the conjoined grafting method through meristem between different phylum, klasse, order, family, genus and species of plantae is provided, and the conjoined grafting method comprises an apcial meristem, a lateral meristem and an intercalary meristem. The meristem possesses massive reversible gene regulatory networks and spatial and temporal expression opportunities due to the fact that the meristem has high cell division ability and is a key tissue of organogenesis and morphogenesis and a starting point of organ and tissue differentiation. Therefore, conducting moderate manual injury intervention and conjoined grafting on the meristem can greatly increase opportunity of successful grafting, and enlarges boundary capable of being conducted grafting between different phylum, klasse, order, family, genus and species of the plantae. Due to the fact that scion and rootstock whole plant are not separated, bud mutation and fructification are promoted to obtain grafting progeny seeds, exchanging and changing of part genetic materials of grafting progeny can be achieved, and consequently, low coat and safe material exchanging of extra distant species of the plantae can be achieved, variation is induced, and then a new germplasm of a scion plant is created.

The invention discloses an agrobacterium-mediated transgenic method for mature seed embryos of corn. The method comprises the following steps of: (1) constructing a plasmid vector expressed by plant genes; (2) constructing an agrobacterium tumefaciens strain containing the plasmid vector expressed by the plant genes in step (1); (3) preparing and wounding bare embryos of mature seeds of the corn; (4) performing genetic transformation on the agrobacterium-mediated, bare and wounded embryos of the mature seeds of the corn in step (2); (5) re-screening the herbicide resistance of regenerated seedlings; (6) transplanting the seedlings which survive after herbicide screening treatment; and (7) performing polymerase chain reaction (PCR) identification on transgenic corn plants. By the method, the problems that the periods of tissue differentiation of maize calluses and plant regeneration are long and the root regeneration is difficult are solved; and the season limitation on the reported transgenic manipulation in which maize rataria is taken as a receptor is effectively avoided, and the time of transformation operation is shortened.

Compositions and methods for inducing tissue differentiation and growth within a localized area of application in humans or other animals are provided. Compositions comprising a biological or pharmacologically active agent such as Insulin-like Growth Factor-1 (IGF-1) and sustained-release nanoparticle formulations comprising IGF-1 and a matrix forming component such as hyaluronan for use in skin repair and regeneration are provided. Nanoparticles of the invention activate endogenous stem cells in the local area of administration to produce new dermal cells and tissues.

The invention provides a high-yield high-quality fast breeding method of sasanqua. The method comprises the following steps of (1) screening breeding parents; (2) hybridizing the breeding parents; harvesting generation F1 hybrid seeds; (3) performing anther induction culture on the generation F1 hybrid seeds; (4) performing callus tissue differentiation culture; (5) performing strong seedling rooting culture; (6) performing domestication transplanting; (7) performing field fixed planting. The anther culture technology is combined with a conventional hybridizing breeding measure to be applied to breeding practice of the sasanqua; sasanqua flower culture regeneration plants are obtained for the first time; through further sasanqua economic index and unsaturated grease quality index screening, the high-yield high-quality sasanqua new strains are successfully obtained. Compared with a pure hybridizing breeding method with dozens of years of breeding period, the method provided by the invention has the advantages that the breeding years are greatly shortened; the breeding efficiency is improved; the sasanqua breeding process is greatly accelerated; the important significance is realizedon the sasanqua improved type breeding and popularization and health and fast development of sasanqua industry.

The invention provides a method for quickly breeding plumeria rubra by tissue culture. The method comprises the following steps of carrying out tissue culture and rapid propagation with MS (Murashige and Skoog) as a minimal medium and plumeria rubra leaves as explants under different hormone components and concentration levels; carrying out sterilization treatment and inoculation induction to form a callus tissue, and then carrying out callus tissue differentiation, bud multiplication and rooting culture to form a complete plant seedling; and finally carrying out acclimatization and transplantation, wherein the minimal medium is based on the MS culture medium, and supplemented with components including 6-benzyl aminoadenine, 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, sucrose, active carbon and the like. According to the invention, tissue culture is applied to overcome the problem that the plumeria rubra is too long in seedling-raising period and low in propagation coefficient, so that factory-like quick seedling raising can be carried out for meeting the demands of the market on the plumeria rubra.

The invention relates to a KZO mineralization fertilizer synergistic additive. According to the technical scheme, the additive comprises the following components in percentage by weight: 80 to 85 percent of attapulgite clay, 8 to 10 percent of DA-6, 1 to 4 percent of silk peptide, 1 to 4 percent of metalloprotease and 0.5 to 2 percent of compound sodium nitrophenolate. The attapulgite clay is activated, and mineral elements are activated by adding a photocatalyst TiO2 and through ultraviolet irradiation. The defect of the conventional chemical fertilizer is overcome, the effect and utilization rate of the chemical fertilizer are improved, various beneficial mineral elements are provided for plants, the cell division and tissue differentiation of the plants are promoted, and the formation of root systems is promoted; and the validity period of the chemical fertilizer can be prolonged, the using amount of the chemical fertilizer can be reduced by over 30 percent, environmental pollution is effectively reduced, and the aims of increasing yield and efficiency and reducing cost are fulfilled.

A multi-cellular virtual tissue having the emergent properties of self-repair, adaptive response to an altered environment, or tissue differentiation, and a method of generating the tissue by computer modeling are disclosed. The tissue is formed of a plurality of virtual cells, each having a heritable virtual genome containing a set of virtual genes relating to each of (a1) intercellular adhesion, (a2) cell division, (a3) cell growth, (a4) intercellular signaling, and (a5) the state of one cell relative to an adjacent cell. In forming the tissue, the sequential operation and actions of the genes are guided by (1) chemical-interaction rules that govern the extra-genetic behavior of one or more molecules placed or produced in the environment, (2) action rules that specify a cell's adhesion, growth, or cell-division condition, in response to molecules produced by a cell's genes relating to intercellular adhesion, cell growth, or cell division, respectively, and (3) physical-interaction rules that govern how a cell will move in response to its own growth or division or the growth or division of neighboring cells.

The invention discloses a tissue culture rapid propagation method for Cerasus xueluoensis C.H.Nan & X.R.Wang. The method comprises the steps of: A, sterilization of leaves and stem sections; B, adventitious bud induction and proliferation; C, anti-browning test; D, callus induction and proliferation; E, test for callus tissue differentiation of bud clusters, seedling strengthening and rooting culture; and G, embryonic callus induction and differential test. By adopting a plant tissue culture method to propagate Cerasus xueluoensis C.H.Nan & X.R.Wang, a large amount of foreign exchange can be saved, and the influence subjected to the outside is relatively less, the method can be performed in the four seasons, the seedling occupied land is saved, and the cost is lowered. Moreover, all excellent characters and hereditary stability of the parent are preserved. The rapid propagation method can be used for performing a large amount of test-tube plantlet scaled industrial production in a short term.

The invention discloses repair spray containing high-concentration human mesenchymal stem cell extracts for a small pet and a preparation method of the repair spray. The repair spray mainly comprises the high-concentration mesenchymal stem cell extracts. Mesenchymal stem cells can secrete various cell factors in the culture process, the cell factors can penetrate into an endomembrane base layer, endomembrane tissue differentiation, vessel regeneration and granulation tissue growth are promoted, injured skin tissue structure regeneration and repair are promoted, generation of scar connective tissues is decreased, a skin immune microenvironment is regulated, and bacterial infection and inflammatory reaction of wound tissues are decreased. By a continuous mesenchymal stem cell starving method, high-concentration human umbilical cord mesenchymal extracts can be obtained in a short time. The repair spray containing the high-concentration mesenchymal stem cell extracts is applicable to repair of eczema, chronic surface ulcers and various skin injuries.

The invention discloses a rapid rooting method for olive cutting slips. A composite rooting solution is used for soaking cutting slips in the cutting process, and materials of gamma-polyglutamic acid,dopamine hydrochloride and nano chitosan and the like are added into the rooting solution on the basis of taking indoleacetic acid as an exogenous hormone growth regulator, so that high-activity gel-like thin films can be formed at the roots of the cutting slips, the absorption efficiency of the roots to the nutrient elements in the soil is improved, the cell tissue differentiation is promoted, and the sprouting of the root system is accelerated. In addition, the effective components in the rooting solution are fixed to the root part, so that the long-term promotion effects of preserving moisture and resisting bacteria are achieved, the adaptability of the roots of the cutting slips in the soil is improved, rich nutrients can be improved, and the cutting survival rate can be notably improved. The rooting rate of the olive cutting slips soaked by the rooting solution can be up to 85%, and the seedling breeding period at a same condition is averagely shortened by 2-3 months.