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High-yield high-quality fast breeding method of sasanqua

A Camellia oleifera, high-quality technology, applied in the field of rapid breeding of high-yield and high-quality Camellia oleifera, can solve the problems of not many, achieve the effect of shortening the breeding period and improving the breeding efficiency of Camellia oleifera

Inactive Publication Date: 2019-01-15
LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many domestic studies on the culture of camellia oleifera anthers, and most of them only focus on the induction of callus by anther culture. Only Long Zhenxiong once reported that ordinary camellia oleifera obtained callus and formed green buds and roots through anther culture. , which shows that there are still many problems in the practice of Camellia oleifera anther culture

Method used

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  • High-yield high-quality fast breeding method of sasanqua

Examples

Experimental program
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Effect test

Embodiment 1

[0030] A method for rapid breeding of high-yield and high-quality camellia oleifera, the method comprising the following steps:

[0031] 1) Screening of breeding parents: According to the breeding goals of high yield and high quality (high yield per plant, high dry seed yield, high fresh seed yield, high oil content of seed kernels, high oleic acid content), we screened out suitable ones for planting in Zhejiang. Changlin 3 and Zhejiang Qingtian 25 were used as breeding parents. The fruit size of Changlin No. 3 is medium. The six-year-old plant has a fruit yield of about 3.0kg per plant, a dry seed yield of 24.0%, and an oil content of 46.8%. The yield is relatively stable. Zhejiang Qingtian No. 25 kernel has an oil content of 52.1%, an unsaturated fatty acid content of 90.6%, and an oleic acid content of 81.3%. The oil quality is excellent.

[0032] 2) Crossbreed the breeding parents and harvest the F1 generation hybrids: In 2007, at the flowering stage of Changlin 3 and Zhe...

Embodiment 2

[0042] In order to prove the effect of the cold plasma pretreatment method of the present invention on the induction and differentiation of Camellia oleifera anthers, this example cancels the cold plasma pretreatment of flower buds in Step 3) of Example 1, and only puts the flower buds at a low temperature of 4°C Pretreatment 6d, other operations were the same as in Example 1, and the callus induction rate and green shoot differentiation rate were counted.

Embodiment 3

[0044] In order to prove the influence of the differentiation medium of the present invention on the differentiation of Camellia oleifera anthers, in this example, the differentiation medium used in Step 4) of Example 1 was replaced by N6+2.0mg / LBA+0.5mg / LIAA+0.5mg / L5Br+ 1.0mg / L riboflavin, the differentiation medium is derived from Long Zhenxiong's "Cultivation of Camellia Camellia Anthers to Obtain Green Buds and Roots", and the green shoot differentiation rate is counted.

[0045] Result analysis

[0046] 1. The influence of different pretreatment methods on anther induction and differentiation of camellia oleifera, the callus induction rate and green shoot differentiation rate of embodiment 1 and embodiment 2 are counted in the following table 1.

[0047]

[0048] As can be seen from the results in the table above, the cold plasma pretreatment method adopted in the present invention can improve the callus induction rate and the green shoot differentiation rate in Camell...

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Abstract

The invention provides a high-yield high-quality fast breeding method of sasanqua. The method comprises the following steps of (1) screening breeding parents; (2) hybridizing the breeding parents; harvesting generation F1 hybrid seeds; (3) performing anther induction culture on the generation F1 hybrid seeds; (4) performing callus tissue differentiation culture; (5) performing strong seedling rooting culture; (6) performing domestication transplanting; (7) performing field fixed planting. The anther culture technology is combined with a conventional hybridizing breeding measure to be applied to breeding practice of the sasanqua; sasanqua flower culture regeneration plants are obtained for the first time; through further sasanqua economic index and unsaturated grease quality index screening, the high-yield high-quality sasanqua new strains are successfully obtained. Compared with a pure hybridizing breeding method with dozens of years of breeding period, the method provided by the invention has the advantages that the breeding years are greatly shortened; the breeding efficiency is improved; the sasanqua breeding process is greatly accelerated; the important significance is realizedon the sasanqua improved type breeding and popularization and health and fast development of sasanqua industry.

Description

technical field [0001] The invention relates to the field of nursery stock breeding, in particular to a rapid breeding method for high-yield and high-quality camellia oleifera. Background technique [0002] Camellia oleifera in a narrow sense refers to ordinary camellia oleifera ( Camellia oleifera Abel.), also known as tea tree, tea oil tree, white flower tea, is a member of the family Theaceae ( Theaceae ) Camellia ( Camellia ), the main morphological feature is a small tree or shrub, with white flowers, terminal, and capsules. It is named for its seeds that can be extracted for oil (tea oil). Camellia oleifera in a broad sense is a general term for species of Camellia oleifera with a high seed oil content and a certain area of ​​cultivation and management in the Theaceae family. Camellia oleifera, Camellia oleifera Gaozhou, Camellia oleifera narrow leaves, Camellia oleifera of Vietnam, Camellia oleifera, Zhejiang safflower camellia, Nanrong camellia, Tengchong saffl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/001A01H4/008
Inventor 许圆君姜磊
Owner LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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