Rapid identification of MLO (mildew resistance locus o) powdery mildew resistance Poncirus trifoliata genes

A powdery mildew resistance gene and powdery mildew resistance technology, applied in the field of plant biotechnology science, can solve problems such as long time and difficult cloning, and achieve the effect of shortening breeding years, shortening time, and improving identification efficiency

Inactive Publication Date: 2012-10-03
常熟市支塘镇新盛技术咨询服务有限公司
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Problems solved by technology

However, because the basic research of trifoliate trifoliate fruit is not deep enough, it is not only time-consuming to use these methods but also it is difficult to accurately clone these genes

Method used

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  • Rapid identification of MLO (mildew resistance locus o) powdery mildew resistance Poncirus trifoliata genes
  • Rapid identification of MLO (mildew resistance locus o) powdery mildew resistance Poncirus trifoliata genes
  • Rapid identification of MLO (mildew resistance locus o) powdery mildew resistance Poncirus trifoliata genes

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Experimental program
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Effect test

Embodiment Construction

[0030] 1) Collection and identification of Hovenia trifoliate MLO gene

[0031] In order to obtain all the members of the MLO-type gene family of Hovenia trifoliate, we first constructed an HMM model based on the MLO-type genes of Arabidopsis thaliana, and the MLO gene sequences of resistance to powdery mildew of tomato, pea, pepper, rose, pepper, and japonicus japonicus. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC3159259, 3 TC TC315944, TC315912, TC322759, TC322059, TC330654, TC282713, TC293173, TC281861, TC283253, TC283383, TC285032, TC290021, TC302716, TC283487, TC282866, TC283441, TC281428, TC285118, TC285090; 来自GenBank数据库:AY967408, AF384145, AF384144,AY029312- AY029315, AY029317-AY029319, Z95352, AF369563-AF369565, AF369567, AF369569-AF369576, Z83834, Z95496, AY581255), select similarit...

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Abstract

The invention relates to rapid identification of powdery mildew resistance Poncirus trifoliata genes, relates to knowledge of subjects such as plant comparative genomics, genetics and bioinformatics, and belongs to the technical field of plant biotechnology. The rapid identification mainly includes the steps of firstly, downloading full genomic sequences of Poncirus trifoliate and collecting MLO genes; secondly, identifying the MLO genes; thirdly, identifying phylogenetic relationship of the MLO genes; and fourthly, comparing the MLO powdery mildew resistance genes. By the rapid identification, mining cycle of the powdery mildew resistance Poncirus trifoliata genes is shortened effectively, and the powdery mildew resistance genes can be identified quickly. Corresponding co-separation functional marks (SNP (single nucleotide polymorphism), Scar and the like) can be developed through candidate powdery mildew resistance genes identified, the rapid identification is also available for molecular marker-assisted selection of the powdery mildew resistance genes, and accuracy is high. The rapid identification can also be used with other resistance gene molecular markers to create multiresistance breeding materials, breeding period is shortened, breeding efficiency is improved, and basis for elaborating powdery mildew resistance Poncirus trifoliata molecular mechanisms is laid.

Description

technical field [0001] The present invention uses the method of plant comparative genomics, genetics, bioinformatics and candidate gene strategies to quickly identify the powdery mildew gene of trifoliate trifoliate by means of sequencing the whole genome sequence of trifoliate trifoliate, and mainly involves the download of the whole genome sequence of trifoliate trifoliate and the identification of candidate genes , gene comparison, clustering and other means, and then identify the powdery mildew gene, which belongs to the field of plant biotechnology science. Background technique [0002] orange( Poncirus trifoliata ), also known as Tielizhai, stinky orange, wolfberry, wolfberry, and stinky berry. It belongs to Rutaceae Kumquat and is a deciduous shrub. It is cultivated in Shaanxi, Gansu, Hebei, Shandong, Jiangsu, Zhejiang, Hunan, Guangdong and other provinces in my country. The whole body of Citrus aurantium is densely covered with thick and long thorns of about 2cm. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12Q1/68
Inventor 钱孝英袁伟
Owner 常熟市支塘镇新盛技术咨询服务有限公司
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