8 results about "Locus (genetics)" patented technology

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.

This invention belongs to the field of animal molecular genetics and breeding technology, specifically relating to SNP molecular marker combinations for identifying kinship in Sunite Mongolian cattle and their applications. The marker combination consists of 141 highly polymorphic SNP loci distributed on bovine autosomes 1-29, with an average physical distance of 19.93 Mb between loci, exhibiting good genome coverage and locus independence. The selected SNP loci show high minimum allele frequency (MAF), expected heterozygosity (He), and polymorphism information content (PIC) in the Sunite Mongolian cattle population, with average values ​​of 0.4843, 0.4990, and 0.3745, respectively, and a cumulative exclusion probability of no less than 0.9999 under unknown maternal genotype conditions. The SNP molecular marker combination and its application method of this invention can provide a reliable molecular tool for the protection of Sunite Mongolian cattle germplasm resources, the construction of conservation populations, the selection of breeding bulls and cows, and the development of the Sunite Mongolian beef cattle industry, offering advantages such as ease of operation, moderate cost, and high accuracy.

This invention discloses cotton GhGot1L This invention relates to genes and their application in creating heat-resistant cotton breeding materials, belonging to the field of cotton genetics and molecular breeding technology. This invention focuses on genetic analysis of the heat-resistant locus qPV-D05, which has been identified in the upland cotton genome. Through gene editing, it was determined that the causal gene at the qPV-D05 locus encodes a Golgi transporter protein. GhGot1L This invention corrects the problem through cloning experiments. GhGot1L Based on the reference genome annotation information, cloned GhGot1L The actual gene coding sequence was obtained, and molecular markers were developed for the heat-resistant genotypes generated by gene editing, which can be used to breed heat-resistant upland cotton germplasm.

The present application relates to a kind of genetic locus related to peanut leaf size, gene and molecular marker, belong to crop molecular genetics and molecular marker technical field.The two genetic loci related to leaf size of the present application are LA115165101 and LA115027188, wherein LA115165101 is located at the interval of 115165101-115165105 of the 5th chromosome of peanut, is base CGCTC or deletion;LA115027188 is located at 115027188 of the 5th chromosome of peanut, base is C or T;Two genes related to leaf size of the present application are Arahy.09KEBU and Arahy.PRU9CS, two functional KASP molecular markers are developed based on the gene, using the molecular marker can efficiently, accurately distinguish the leaf size trait of peanut, can effectively distinguish large leaf strain and small leaf strain in population, with the characteristics of simple operation, reliable result, low cost, suitable for large-scale molecular marker assisted selection, provide important technical support for cultivating ideal leaf shape, high yield and stable yield of new peanut variety.

This invention discloses a SNP molecular genetic marker for the WNT6 gene related to feed conversion ratio in chickens and its application, belonging to the fields of animal genetics and breeding and molecular biology. The aforementioned SNP molecular genetic marker is located at locus 22401119 of the rs733687399 sequence on chicken chromosome 7, and the locus is a T>G mutation. On the one hand, this invention provides the SNP molecular genetic marker chr7-22401119, which is significantly correlated with the feed conversion ratio trait in yellow-feathered broilers. It can be applied to the breeding of yellow-feathered broilers, allowing for the selection of yellow-feathered broilers with low feed conversion ratios, effectively reducing feed consumption during production and improving the economic benefits and competitiveness of enterprises. On the other hand, this invention also provides primers and a kit for identifying the SNP molecular genetic marker chr7-22401119, which can be used for efficient screening of individuals or parents with excellent feed conversion ratio traits in yellow-feathered broilers.

This invention discloses a SNP molecular genetic marker for the NFE2L1 gene related to feed conversion ratio in chickens and its application, belonging to the fields of animal genetics and breeding and molecular biology. The aforementioned SNP molecular genetic marker is located at locus 6493284 on chromosome 27, with the sequence number rs731344472, and is a G>T mutation. The SNP molecular genetic marker chr27-6493284 provided by this invention has been experimentally verified to be significantly associated with the feed conversion ratio trait in yellow-feathered broilers. It can be applied to the breeding of yellow-feathered broilers with low feed conversion ratios, effectively reducing feed consumption during production and improving the economic benefits and competitiveness of enterprises. Primers and kits for SNP molecular genetic marker identification can be used for efficient screening of individuals or parents with excellent feed conversion ratio traits in yellow-feathered broilers.

This invention relates to the field of molecular genetics and breeding technology, and in particular to a SNP molecular marker associated with body weight in beef cattle and its application. This invention utilizes a beef cattle promoter breeding chip to conduct GWAS studies on a beef cattle population, discovering an SNP locus highly correlated with body weight. This locus is located at position 71001780 on chromosome 17 of the bovine reference genome, exhibiting G / A polymorphism. The upstream and downstream nucleotide sequences are as shown in SEQ ID NO.1. Beef cattle with the genotype AA at this SNP locus have higher body weights than those with the genotypes GG or GA, indicating that the A allele is a superior allele associated with higher body weight. The SNP molecular marker provided by this invention can accurately and efficiently identify the growth potential of individual beef cattle, and has significant value in early selection and breeding programs for beef cattle.

The invention belongs to the technical field of aquaculture molecular genetics detection, and particularly relates to a specific SNP site combination and method for grass carp genetic sex identification and application. The SNP sites are located on a chromosome 2 of the grass carp, and the physical positions of the SNP sites are 29859892 and 29859893 respectively; when the genotype of the SNP site is a homozygous deletion type, the individual grass carp is female; when the genotype of the SNP site is a homozygous type, the individual grass carp is male. On the basis, the invention provides a method for carrying out sex identification by directly detecting the site, the sex identification can be accurately and quickly carried out on early grass carp only by a small amount of tissues without dissection, the accuracy is extremely high, and a core technical means is provided for grass carp sex control breeding and efficient culture.