15 results about "Locus (genetics)" patented technology

The invention discloses a molecular marker closely linked with peanut yield related traits and application, and belongs to the technical field of plant molecular genetics and molecular breeding. The molecular marker disclosed by the invention is Arahy0036; the nucleotide sequence of Arahy0036 in peanuts with low single plant yield is shown as SEQ ID NO: 1, and the nucleotide sequence of Arahy0036 in peanuts with high single plant yield is shown as SEQ ID NO: 2. The marker is used for detecting the gene locus related to the yield character of the peanut single plant, the single plant with high yield can be identified after seeds are harvested, other single plants are selected, reserved and eliminated, the breeding scale can be effectively controlled, and the progress and efficiency of variety selection breeding are improved.

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.

This invention belongs to the field of animal molecular genetics and breeding technology, specifically relating to SNP molecular marker combinations for identifying kinship in Sunite Mongolian cattle and their applications. The marker combination consists of 141 highly polymorphic SNP loci distributed on bovine autosomes 1-29, with an average physical distance of 19.93 Mb between loci, exhibiting good genome coverage and locus independence. The selected SNP loci show high minimum allele frequency (MAF), expected heterozygosity (He), and polymorphism information content (PIC) in the Sunite Mongolian cattle population, with average values ​​of 0.4843, 0.4990, and 0.3745, respectively, and a cumulative exclusion probability of no less than 0.9999 under unknown maternal genotype conditions. The SNP molecular marker combination and its application method of this invention can provide a reliable molecular tool for the protection of Sunite Mongolian cattle germplasm resources, the construction of conservation populations, the selection of breeding bulls and cows, and the development of the Sunite Mongolian beef cattle industry, offering advantages such as ease of operation, moderate cost, and high accuracy.

The invention belongs to the technical field of plant molecular genetics, and particularly discloses an InDel molecular marker related to the short fruit character of bitter gourd fruits and application of the InDel molecular marker. According to the InDel molecular marker, a long-fruit bitter gourd inbred line '23C57' and a short-fruit bitter gourd inbred line '23C58' are selected as parent materials, a genetic population containing four generations (P1, P2, F1 and F2) is constructed, and the molecular marker is obtained. A BSA (Bovine Serum Albumin) positioning and linkage positioning combined method is used for detecting and controlling genetic loci of bitter gourd fruit types, and InDel are respectively identified at a 22136954 locus and a 22076609 locus of a bitter gourd No.6 chromosome through sequencing analysis and are respectively named as L070 and L901. The invention provides a theoretical basis for molecular breeding of bitter gourd fruit types, and enriches molecular marker resources.

This invention discloses cotton GhGot1L This invention relates to genes and their application in creating heat-resistant cotton breeding materials, belonging to the field of cotton genetics and molecular breeding technology. This invention focuses on genetic analysis of the heat-resistant locus qPV-D05, which has been identified in the upland cotton genome. Through gene editing, it was determined that the causal gene at the qPV-D05 locus encodes a Golgi transporter protein. GhGot1L This invention corrects the problem through cloning experiments. GhGot1L Based on the reference genome annotation information, cloned GhGot1L The actual gene coding sequence was obtained, and molecular markers were developed for the heat-resistant genotypes generated by gene editing, which can be used to breed heat-resistant upland cotton germplasm.

The present application relates to a kind of genetic locus related to peanut leaf size, gene and molecular marker, belong to crop molecular genetics and molecular marker technical field.The two genetic loci related to leaf size of the present application are LA115165101 and LA115027188, wherein LA115165101 is located at the interval of 115165101-115165105 of the 5th chromosome of peanut, is base CGCTC or deletion;LA115027188 is located at 115027188 of the 5th chromosome of peanut, base is C or T;Two genes related to leaf size of the present application are Arahy.09KEBU and Arahy.PRU9CS, two functional KASP molecular markers are developed based on the gene, using the molecular marker can efficiently, accurately distinguish the leaf size trait of peanut, can effectively distinguish large leaf strain and small leaf strain in population, with the characteristics of simple operation, reliable result, low cost, suitable for large-scale molecular marker assisted selection, provide important technical support for cultivating ideal leaf shape, high yield and stable yield of new peanut variety.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for regulating and controlling the length of a corn kernel and application of the SNP molecular marker, and belongs to the technical field of molecular genetics. Based on genome-wide association analysis (GWAS), SNP (single nucleotide polymorphism) sites (C / T variation) remarkably related to grain length are identified at chromosome 8 74, 366 and 816bp of a corn B73RefGenv5 genome, and a C allele is a dominant allele. And the nucleotide sequences of the KASP primer group for the locus typing are shown as SEQ ID NO: 1-3. The molecular marker is combined with a KASP technology, high-precision and high-throughput genetic typing detection can be realized, and the molecular marker has the characteristics of good amplification stability and high detection efficiency, is suitable for early prediction of corn kernel length, germplasm resource identification and molecular marker-assisted selection breeding, and has important application value for breeding of new long-grain corn varieties.

The invention provides an MNP marker site for identifying a rapid growth type litopenaeus vannamei population, a primer group and application of the MNP marker site and the primer group, and belongs to the technical field of molecular genetics of aquatic animals. The MNP marker site, the corresponding primer group and the kit thereof can screen a litopenaeus vannamei population with the character of high growth speed, and prove that the MNP marker site can realize high-throughput analysis, avoid the generation of a large number of nonexistent error genotypes, reduce the detection cost and analysis complexity, and improve the detection efficiency. And the accuracy rate of identifying the rapid growth type litopenaeus vannamei population is up to 100%. The MNP marker site and application provided by the invention have reference significance in the related fields of molecular inheritance and genotype analysis of aquatic animals.

This invention discloses a SNP molecular genetic marker for the WNT6 gene related to feed conversion ratio in chickens and its application, belonging to the fields of animal genetics and breeding and molecular biology. The aforementioned SNP molecular genetic marker is located at locus 22401119 of the rs733687399 sequence on chicken chromosome 7, and the locus is a T>G mutation. On the one hand, this invention provides the SNP molecular genetic marker chr7-22401119, which is significantly correlated with the feed conversion ratio trait in yellow-feathered broilers. It can be applied to the breeding of yellow-feathered broilers, allowing for the selection of yellow-feathered broilers with low feed conversion ratios, effectively reducing feed consumption during production and improving the economic benefits and competitiveness of enterprises. On the other hand, this invention also provides primers and a kit for identifying the SNP molecular genetic marker chr7-22401119, which can be used for efficient screening of individuals or parents with excellent feed conversion ratio traits in yellow-feathered broilers.

This invention discloses a SNP molecular genetic marker for the NFE2L1 gene related to feed conversion ratio in chickens and its application, belonging to the fields of animal genetics and breeding and molecular biology. The aforementioned SNP molecular genetic marker is located at locus 6493284 on chromosome 27, with the sequence number rs731344472, and is a G>T mutation. The SNP molecular genetic marker chr27-6493284 provided by this invention has been experimentally verified to be significantly associated with the feed conversion ratio trait in yellow-feathered broilers. It can be applied to the breeding of yellow-feathered broilers with low feed conversion ratios, effectively reducing feed consumption during production and improving the economic benefits and competitiveness of enterprises. Primers and kits for SNP molecular genetic marker identification can be used for efficient screening of individuals or parents with excellent feed conversion ratio traits in yellow-feathered broilers.

This invention relates to the field of molecular genetics and breeding technology, and in particular to a SNP molecular marker associated with body weight in beef cattle and its application. This invention utilizes a beef cattle promoter breeding chip to conduct GWAS studies on a beef cattle population, discovering an SNP locus highly correlated with body weight. This locus is located at position 71001780 on chromosome 17 of the bovine reference genome, exhibiting G / A polymorphism. The upstream and downstream nucleotide sequences are as shown in SEQ ID NO.1. Beef cattle with the genotype AA at this SNP locus have higher body weights than those with the genotypes GG or GA, indicating that the A allele is a superior allele associated with higher body weight. The SNP molecular marker provided by this invention can accurately and efficiently identify the growth potential of individual beef cattle, and has significant value in early selection and breeding programs for beef cattle.

The invention belongs to the technical field of plant molecular genetics, and particularly discloses an InDel molecular marker for bitter gourd long-fruit character identification and application thereof.The InDel molecular marker is characterized in that a long-fruit bitter gourd inbred line '23C57' and a short-fruit bitter gourd inbred line '23C58' are selected as parent materials, four genetic populations containing three generations (P1 (23C57), P2 (23C58), F1 and F2) are constructed, and the genetic populations are constructed; a BSA (Bovine Serum Albumin) positioning and linkage positioning combined method is used for detecting and controlling genetic loci of melon and fruit types and developing molecular markers closely linked with the genetic loci. The InDel molecular marker site excavated by the invention is different from an annotation gene of a homologous long fruit gene reported in cucurbitaceae species in the past, a theoretical basis is provided for molecular breeding of melon and fruit types, and molecular marker resources are enriched.

The invention belongs to the technical field of aquaculture molecular genetics detection, and particularly relates to a specific SNP site combination and method for grass carp genetic sex identification and application. The SNP sites are located on a chromosome 2 of the grass carp, and the physical positions of the SNP sites are 29859892 and 29859893 respectively; when the genotype of the SNP site is a homozygous deletion type, the individual grass carp is female; when the genotype of the SNP site is a homozygous type, the individual grass carp is male. On the basis, the invention provides a method for carrying out sex identification by directly detecting the site, the sex identification can be accurately and quickly carried out on early grass carp only by a small amount of tissues without dissection, the accuracy is extremely high, and a core technical means is provided for grass carp sex control breeding and efficient culture.

The invention relates to a reference-free genome species individual identification method for simple genome assembly, and belongs to the technical field of bioinformatics and genetics. Aiming at species lacking a reference genome, the invention provides a complete individual identification process which comprises the steps of assembling a simple genome, constructing a population SNP site library and performing individual identification verification on low-depth sequencing data. The reference-free genome species individual identification method for simple genome assembly solves the problem that a traditional RFID chip is insufficient in individual identity identification, and also solves the problems that existing molecular identification mainly depends on STR and SNP site amplification, a large number of specific primers need to be designed and repeatedly optimized in the earlier stage, and the process is tedious and time-consuming. Meanwhile, for species without high-quality reference genomes, a convenient, efficient and low-cost individual identification method is established, and the method is suitable for individual tracking of endangered species and research of non-mode species and has high universality and practicability.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker for identifying fat tail type sheep and application of the SNP marker, and the SNP molecular marker is located at the 19248082nd nucleotide site G / T mutation on the No.3 chromosome of AR-UIRamv3.0 reference genome (GCF016772045.2). The invention also relates to a detection method for detecting the SNP molecular marker by using a PCR technology. The technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. According to the invention, SNP locus detection is used for carrying out fat-tail type sheep early selection, shortening the breeding period and accelerating the breeding process, an fat-tail type sheep early selection technology is established, the breeding time of excellent traits of fat-tail type sheep is shortened, the breeding cost is reduced, and the application value is very high.