Multiplex amplification system and kit for 23 short tandem repeats

A compound amplification system and short tandem repeat technology, applied in the biological field to achieve high system efficiency and good independence

Active Publication Date: 2015-10-21
SUZHOU MICROREAD GENETICS
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] Most of the kits currently used are 13 CODIS (Combined DNA Index System) selected by the US FBI: CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11 are Basic CODIS locus kit, but in some assays, more non-CODIS loci are needed to provide more information

Method used

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  • Multiplex amplification system and kit for 23 short tandem repeats
  • Multiplex amplification system and kit for 23 short tandem repeats
  • Multiplex amplification system and kit for 23 short tandem repeats

Examples

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Embodiment 2

[0121] Example 2 Application of 23 short tandem repeats composite amplification kit for paternity testing

[0122] 1. Collect blood samples for paternity testing (blood samples are provided by a genetic testing center)

[0123] 2. Use Chelex-100 method to extract genomic DNA (refer to "Forensic DNA Protocol". Humana Press, 1998), take 0.5-5μl of anticoagulated whole blood or 1-3mm×2-5mm blood spots into a 500μl centrifuge tube, shake Mix the Chelex solution to make the Chelex fully suspended, add 195μl Chelex-100 (5%) solution and 5μl proteinase K (20mg / ml) to each tube, shake and mix well, incubate at 56°C for two hours or overnight, take out and shake for 2 minutes, boil water After heating for 10 minutes, centrifuge at 13000 rpm for 5 minutes, and carefully transfer 150 μl of supernatant to a new centrifuge tube.

[0124] 3. Perform PCR amplification according to the amplification conditions in Example 1.

[0125] 4. Perform the test on the genetic analyzer in accordance with Exam...

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Abstract

The invention relates to a composite amplification system of 23 short tandem repeat sequences and a kit, is used for detecting heredity mark gene of polymorphism in a mankind genome, and belongs to the biology technical field. The invention relates to a scheme that a plurality of short tandem repeat sequences are simultaneously amplified in a PCR system; a specific gene locus comprises 22 short tandem repeat sequences with high level heredity polymorphism and 1 gender determination locus; primers are respectively designed and a fluorophore is marked; the system has very high individual recognition rate and non-father elimination rate; the system and kit can be used for legal medical individual recognition, paternity tests, and colony genetics analysis, is high in accuracy, and good in sensitivity.

Description

Technical field [0001] The invention belongs to the field of biotechnology and relates to a composite amplification system for identifying multiple short tandem repeats in humans, which can quickly and accurately perform human STR typing and provide a basis for forensic individual identification and paternity identification. Background technique [0002] Human genome STR (Short Tandem Repeats, short tandem repeats) is a relatively stable sequence in DNA inheritance formed by tandem repeats of several bases as the core unit. Different races, different populations, and different individuals are distinguished by differences in core unit sequences and repeat numbers, which also constitute the genetic polymorphism of STR. In the genome, there is an average of one STR site every 15-20kb, accounting for 10% of the genome. It is mostly found in non-coding regions and introns. The repeat unit is 2-6bp, the number of repeats is 10-60 times, and the fragment size Between 70~500bp, and acco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 陈初光于在亮宋欣欣付亮剑
Owner SUZHOU MICROREAD GENETICS
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