Multiplex amplification system and kit for 23 short tandem repeats

Abstract

The invention relates to a composite amplification system of 23 short tandem repeat sequences and a kit, is used for detecting heredity mark gene of polymorphism in a mankind genome, and belongs to the biology technical field. The invention relates to a scheme that a plurality of short tandem repeat sequences are simultaneously amplified in a PCR system; a specific gene locus comprises 22 short tandem repeat sequences with high level heredity polymorphism and 1 gender determination locus; primers are respectively designed and a fluorophore is marked; the system has very high individual recognition rate and non-father elimination rate; the system and kit can be used for legal medical individual recognition, paternity tests, and colony genetics analysis, is high in accuracy, and good in sensitivity.

Description

Technical field

[0001] The invention belongs to the field of biotechnology and relates to a composite amplification system for identifying multiple short tandem repeats in humans, which can quickly and accurately perform human STR typing and provide a basis for forensic individual identification and paternity identification. Background technique

[0002] Human genome STR (Short Tandem Repeats, short tandem repeats) is a relatively stable sequence in DNA inheritance formed by tandem repeats of several bases as the core unit. Different races, different populations, and different individuals are distinguished by differences in core unit sequences and repeat numbers, which also constitute the genetic polymorphism of STR. In the genome, there is an average of one STR site every 15-20kb, accounting for 10% of the genome. It is mostly found in non-coding regions and introns. The repeat unit is 2-6bp, the number of repeats is 10-60 times, and the fragment size Between 70~500bp, and acco...

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