54 results about "Tandem Repeat Sequence" patented technology

The present invention provides a method for constructing a plasmid containing a tandem repeat sequence, comprising: providing a first upstream primer and a first downstream primer; the first upstream primer and the first downstream primer respectively have a tandem repeat sequence, and the tandem repeat sequence of the first upstream primer is complementary to at least part of the tandem repeat sequence in the tandem repeat sequence of the first downstream primer; annealing the first upstream primer and the first downstream primer under a gradient temperature condition to obtain a first double-stranded nucleic acid fragment having a tandem repeat sequence, wherein the tandem repeat sequence complementary to each other in the double strand is the first double-stranded tandem repeat sequence; connecting the first double-stranded nucleic acid fragment to a first vector backbone with a sticky end to construct a plasmid containing the first double-stranded tandem repeat sequence. The present invention can significantly improve the success efficiency of tandem repeat sequence connection, obtain more positive clones, ensure the success rate of the experiment, and reduce the construction time.

The invention relates to an mRNA (messenger Ribonucleic Acid) vaccine targeting FomA and application of the mRNA vaccine in immunotherapy of esophageal squamous carcinoma in the technical field of tumor immunotherapy. The problems that in the prior art, a specific targeting vaccine for the fusobacterium nucleatum membrane protein FomA lacks, traditional antibiotics can only kill extracellular bacteria and cannot remove intracellular colonized fusobacterium nucleatum, and cellular immunity cannot be effectively activated to remove fusobacterium nucleatum colonized in tumor tissue; the immunotherapy drug resistance of esophageal squamous carcinoma caused by fusobacterium nucleatum infection is difficult to reverse. According to the technical scheme, an mRNA molecule with a fusobacterium nucleatum FomA extracellular domain tandem repeat sequence and a lipid nanoparticle delivery system are coded, an extramembrane segment tandem repeat immune enhancement technology is adopted for design, two extracellular ring sequences are repeated for 2-5 times and connected through a connector sequence, lipid nanoparticles are composed of optimized quaternary lipids, and the lipid nanoparticles are prepared from the quaternary lipids. And a PD-1 monoclonal antibody is combined to activate FomA specific CD8 + T cell immunoreaction, so that comprehensive removal of intracellular and extracellular bacteria and bacteria targeted immune remodeling are realized.

Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding the expression product or products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.

The invention provides a microsatellite site stability detection method and an electronic device thereof. The microsatellite locus stability detection method comprises the following steps: S1) utilizing high-throughput targeted sequencing data of a sample to be detected to obtain feature data in a preset microsatellite locus set, the feature data at least comprising length variation of a short tandem repeat sequence and structural variation occurring at a microsatellite locus; according to the microsatellite stability detection method and device, the problem that in the prior art, a method for detecting microsatellite instability has limitation can be solved, and the microsatellite stability detection method and device are suitable for the field of tumor detection.

The present invention belongs to the field of biotechnology, and relates to a composite amplification system and a kit of 23 short tandem repeat sequences, which are used to detect genetic marker genes with polymorphism in the human genome, and belongs to the field of biotechnology. The present invention relates to amplifying multiple short tandem repeat sequences simultaneously in a PCR system, and the specific loci are 22 short tandem repeat sequences with high genetic polymorphism and 1 sex determination site, and primers are designed and labeled with fluorescent groups respectively. The system has extremely high individual recognition rate and non-paternal exclusion rate, large amount of information, and good compatibility. The present invention can be used for forensic individual identification, paternity testing, and population genetics analysis, with higher accuracy and good sensitivity.

The invention provides a primer group, a kit and a detection method for individual identification of treponema pallidum, the primer group comprises 12 pairs of primers with nucleotide sequences respectively corresponding to SEQ ID NO.1-SEQ ID NO.24. The invention provides a specific DNA fingerprint spectrum of treponema pallidum strains formed by 12 loci, a variable number tandem repeat sequence (microsatellite sequence) of a genome is analyzed, and the specific DNA fingerprint spectrum of the treponema pallidum strains is obtained. The method is used for realizing individual identification of treponema pallidum strains. The primer group provided by the invention has high sensitivity, accuracy and specificity, and the detection method based on the primer group can carry out individual identification, genetic identification and genetic typing analysis at the same time, and has wide application prospects in the fields of population genetics and the like.

The invention provides an ethylene biosynthesis RhACO4 gene for regulating and controlling the aging of Chinese rose flowers and application of the ethylene biosynthesis RhACO4 gene, and belongs to the technical field of gene engineering. The coding nucleotide sequence of the RhACO4 gene is as shown in SEQ ID NO. 1. According to the invention, the fact that two genotypes (ProRhACO4A and ProRhACO4B) exist in an RhACO4 promoter region in 358 tetraploid Chinese rose populations is found for the first time, and the difference is that whether a section of GA dinucleotide tandem repeat sequence (GA-peak) which is 11 times is contained or not. The ProRhACO4A type carrying GA-peak is significantly related to a lower gene expression level, a lower endogenous ethylene synthesis amount and a longer vase life. The invention provides a key gene target, a molecular marker and a technical path for cultivating a new variety of rosa chinensis with a long flowering period through molecular marker-assisted selection or genetic engineering means.

The present invention provides a polynucleotide comprising, in a 5' to 3' direction, a 5' untranslated region (5'-UTR) and an open reading frame (ORF), wherein the 5'-UTR comprises at least two tandem repeats of the sequence 5'-GCCNCC-3' operably linked to the ORF, where N is any nucleotide. The present invention also provides compositions comprising lipid nanoparticles and polynucleotides, as well as pharmaceutical compositions and their use in medicines, particularly for use as vaccines or in gene therapy.

The invention relates to the technical field of genomics and microbial science, in particular to a myxobacteria T2T genome assembling method. The assembling method mainly comprises the four steps of global assembling, telomere sequence screening, local sequence clustering screening and second-generation data multi-round correction. According to the method provided by the invention, pollution removal strategies of telomere sequence screening and local sequence clustering screening are newly added, so that the problem that tandem repeat sequences in a myxobacteria genome are complex and short is effectively solved, the analysis of a complex region becomes possible, the integrity and continuity of assembly are improved, and the assembly efficiency is improved. The method does not need fingerprint spectrum, Hi-C technology or optical spectrum sequencing technology, reduces the experiment cost, ensures that all the sequences belonging to myxobacteria are extracted, effectively removes the interference of repetitive sequences and deep pronucleus pollution, enables highly similar repetitive sequence fragments to be reduced and positioned to an accurate genome position, and improves the accuracy of the detection result. The integrity and continuity of the genome are obviously improved.

To provide a method for comparing genetic identity, and to provide a method for determining the identity of individuals from which multiple samples are derived.SOLUTION: Some embodiments of the present disclosure provide a method for comparing the identities of genes, which includes comparing the identities between human leukocyte antigen genes, short tandem repeat sequences, and killer cell immunoglobulin-like receptor genes of a test sample and a target gene group, and determining that the target gene group is identical to the test gene group of the test sample if comparison results of the human leukocyte antigen genes, comparison results of the short tandem repeat sequences, and comparison results of the killer cell immunoglobulin-like receptor genes are all identical. Some embodiments of the present disclosure further provide a method for determining the identities of individuals from whom multiple samples are derived.SELECTED DRAWING: Figure 1

The application provides a microsatellite site stability detection method and an electronic device thereof. The microsatellite site stability detection method comprises the following steps: S1, obtaining characteristic data in a preset microsatellite site set by using high-throughput targeted sequencing data of a to-be-detected sample, wherein the characteristic data at least comprises the following: length variation of a short tandem repeat sequence and structural variation occurring at the microsatellite site; S2, establishing a prediction model by using the characteristic data; and S3, outputting a microsatellite stability result of the to-be-detected sample by using the prediction model. The method can solve the limitation problem of the microsatellite instability detection method in the prior art and is suitable for the tumor detection field.

The invention relates to a forensic physical evidence multi-person identity authentication method. The method comprises the following steps: S1, optimizing a short tandem repeat map mathematical model; s2, preprocessing the short tandem repeat map mathematical model to obtain sites containing information; s3, using a mean shift algorithm to process peak points in the atlas, converging to a density local maximum point, and outputting the number of modes as the preliminary estimation of the number of contributors of the locus; s4, judging the mixing ratio of each contributor by using a gradient descent algorithm; and S5, generating an initial vector depending on the mixing ratio according to the mixing ratio, performing sliding matching by keeping the contribution amount of the contributor unchanged, finding an allele size value suitable for genome information contributed by each contributor, and generating a genotype by searching an id corresponding to the nearest allele size in an original map. By means of the design, DNA information of multiple persons can be processed, and contributor composition and proportion of all components in mixed data are analyzed.

The invention relates to a group of Y-chromosomal short tandem repeat (Y-STR) markers comprising at least one rapidly mutating (RM) Y-STR marker selected from the group consisting of DYF1000, DYF1001, DYF1002, DYR88, DYS685, DYS688, DYS712, DYS1003, DYS1007, DYS1010 and DYS1012. The invention further relates to a set of amplification primers comprising primers for the amplification of at least one Y-STR marker according to the invention, to methods for amplifying an allele of at least one Y-STR marker, to a kit for identifying an allele of a Y-STR marker by amplification and electrophoretic detection or sequencing detection, and by sequencing of non-amplified DNA, and to the use of the group of Y-STR markers for typing male individuals.

This invention relates to the field of bioinformatics analysis of sequencing data, specifically providing a genotyping method for short tandem repeat sequences based on next-generation sequencing. The detection algorithm considers various positional relationships between sequencing read lengths and STR regions, models each case separately, eliminates the effects of partial sequence mismatches, insertions, and deletions, and integrates all read information to calculate the optimal STR genotyping result that best matches the observation. This method can be used for genotyping detection of short tandem repeat sequences in the human genome.

The invention belongs to the technical field of cellular neurobiology, and relates to a method for capturing a dynamic interaction group of fusion protein and intracellular amyloid protein and application of the method. In the capturing method, the fusion protein is formed by fusing tandem repeat amyloid protein and proximity marker enzyme through flexible connecting peptide; through the design of the tandem repeat sequence of the amyloid A beta, the flexible connecting peptide, the proximity marker enzyme and the like, the local multivalence of the protein is remarkably improved, the saturation concentration required by phase separation is further reduced exponentially, more and more lasting liquid condensates can be stably formed in cells, and the phase separation efficiency is improved. Thus, the metastable oligomer droplets formed by the liquid aggregate are stabilized under physiological conditions. Fluorescence bleaching recovery verifies that the agglomerate generated by the method has pathological liquid-solid phase change characteristics (no fluorescence recovery), and successfully simulates the aging process of amyloid protein. The invention provides a powerful tool for researching a phase separation mediated pathogenic mechanism in amyloid protein related neurodegenerative diseases (such as Alzheimer's disease) and screening a therapeutic drug for agglutinate aging or protein hijacking.

The application discloses a method and device for short tandem repeat sequence typing based on third-generation sequencing data and a storage medium. The method for short tandem repeat sequence typing based on third-generation sequencing data provided by the application first extracts all read segments completely covering the short tandem repeat sequence region according to a short tandem repeat sequence site directory; and filters the read segments by average alignment quality to remove low-quality read segments; then calculates the copy number of the short tandem repeat sequence in each read segment, and combines mutation site information of the short tandem repeat sequence to confirm short tandem repeat sequence typing. The method provided by the application can accurately calculate the repetition number of the short tandem repeat sequence, and combines the mutation site to determine the short tandem repeat sequence typing, so that more accurate genotyping results can be provided. Moreover, the method provided by the application is suitable for third-generation sequencing data of different sequencing platforms, has strong applicability, and can maximize the advantages of the third-generation sequencing read length.

The invention discloses a gene expression enhancing element capable of improving a gene transcription level and an application of the gene expression enhancing element. It is found that a delta satellite region from sweet potato mosaic virus (SPLCV) SBG51 comprises a new DNA sequence capable of remarkably enhancing expression of a reporter gene, the gene expression enhancing effect of the delta satellite region does not change polyadenylation of mRNA, the delta satellite region is independent of a promoter and a terminator, and the space-time mode of the promoter is kept. The invention further finds that AT-rich sequences (such as ATAAA or TTAAA or tandem repeat sequences containing a plurality of ATAAA or TTAAA) are key elements of the above effects, even synthetic AT-rich sequences (AT-rich-1 and AT-rich-2) of 29bp are sufficient to increase the expression of the reporter gene GFP by about 3 times, and the gene expression enhancement effect of the AT-rich sequences is well expressed in both plants and yeast cells. Therefore, the invention can be applied to the aspects of improving the activity of the target gene according to the required level, providing high-level and long-lasting protein production and the like.

Provided is use of the watermelon flesh color intensity control gene C1FCI and a promoter region tandem repeat sequence thereof in identifying watermelon flesh color intensity. Aiming at the presence of the C1FCI gene promoter tandem repeat region in watermelon varieties, a PCR marker that is convenient to detect is designed, enabling rapid identification of possible flesh color phenotypes in the hybrid progeny of watermelons with light and dark flesh at the seedling stage. When applied in assisted breeding, the gene marker can improve watermelon fruit quality.

The present invention provides a polynucleotide comprising a 5'untranslated region (5 '-UTR) and an open reading framework (ORF) in the 5'to 3' direction wherein the 5 '-UTR comprises at least two tandem repeat sequences 5'-GCCNCC-3 'operatively linked to the ORF, and wherein N is any nucleotide. The invention also provides a composition and a pharmaceutical composition comprising the lipid nanoparticles and the polynucleotide, and their use in medicine, in particular as a vaccine or for gene therapy.

To provide systems and methods for reliably controlled expression of recombinant products in both in vitro and in vivo expression systems, especially with minimal labor for preparation.SOLUTION: Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding one or more expression products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.SELECTED DRAWING: None

The invention discloses three tandem repeat PAV markers related to thousand seed weight of highland barley and application of the three tandem repeat PAV markers. The three PAV markers adopt an oligonucleotide probe sleeve # 8 as a probe, detection is carried out through chromosome fluorescence in situ hybridization in the mitosis metaphase of root tip cells, variations with repetitive sequence insertion fragments are respectively named as 1H-C, 3H-A and 6H-2, and types without insertion fragments are respectively named as 1H-D, 3H-B and 6H-1. Results show that the thousand seed weights of varieties containing 1H-C, 3H-A and 6H-2 are obviously higher than those of other polymorphic chromosomes respectively. The invention provides a cytological marker for thousand seed weight detection for highland barley breeding, and is beneficial to improving highland barley marker-assisted selective breeding efficiency. The influence of chromosome structure variation on the thousand seed weight of highland barley is disclosed, and important information is provided for mining environment adaptability genes and gene clusters.

This invention relates to the field of biotechnology, specifically to a MiniSTR fluorescent multiplex amplification system and kit containing 18 short tandem repeat sequences. It discloses primer sequences targeting 17 autosomal STR loci and 1 sex locus. The core PCR products of these specific amplification primers are all less than 300 bp, increasing the number of loci detected in degraded samples. This system offers advantages such as speed, high sensitivity, and adaptability for detecting trace amounts or degraded samples, making it suitable for highly degraded or inhibitory samples.

Nucleic acid preparation and analysis techniques described. In an embodiment, techniques for generating tandem repeats include using adaptor sets having two types of adaptors with respective complementary regions present. When an adaptor of each type is present on end of a nucleic acid fragment, the complementary regions can bind to one another to generate tandem repeats of an insert, e.g., a fragment generated from a target nucleic acid.

The invention belongs to the technical field of microbial engineering, and particularly relates to an engineering bacterium for efficiently expressing tandem repeat protein as well as a construction method and application of the engineering bacterium. By optimizing precursor amino acid supply, the engineering bacterium for efficiently expressing tandem repeat protein is provided, GHK tripeptide tandem protein and mussel mucin are taken as examples, the yield of tandem repeat protein expressed by the engineering bacterium is increased by enhancing a biosynthetic pathway of an amino acid substrate, the expression bottleneck is relieved, and the expression efficiency is improved. The intracellular synthesis efficiency of the target peptide is remarkably improved, and the production cost is reduced. On the basis of the prior art, the method focuses on yield increase, and provides a solution with higher industrial potential for efficient biosynthesis of oligopeptide.

The invention belongs to the technical field of genetic engineering, and particularly provides a recombinant human-like collagen polypeptide and a preparation method and application thereof, the recombinant human-like collagen polypeptide comprises an N-terminal tandem repeat sequence and a C-terminal sequence; the repetitive sequence is as shown in SEQ ID NO. 1; 1, and the C-terminal sequence is as shown in SEQ ID NO. 2. The recombinant human-like collagen polypeptide provided by the invention can correctly express active collagen with a triple helix structure in a prokaryotic expression system, so that the production cost of the active human collagen is greatly reduced, and the production efficiency is improved; compared with natural human I-type collagen, the prepared recombinant human-like collagen polypeptide has better water retention capacity and degradation resistance, and shows a cell migration promoting effect superior to that of a commercially available collagen product in a cell scratch experiment.

The present invention belongs to the field of recombinant protein engineering, and specifically relates to a biosynthesis method of a recombinant transdermal cyclic peptide and a transdermal absorption application. The biosynthesis method of the recombinant transdermal cyclic peptide of the present invention comprises the following steps: (1) constructing a tandem repeat sequence of a TDP gene fragment into an Escherichia coli expression vector, and transferring it into Escherichia coli for expression, so as to obtain a recombinant transdermal cyclic peptide; (2) releasing the TDP fragment by pepsin cleavage. The present invention provides an effective method for synthesizing cTDP in an engineered bacterial recombinant expression system. The method cleverly introduces a pepsin site, has a high enzymatic cleavage efficiency, can use low-cost pepsin to achieve polypeptide enzymatic cleavage, and does not produce redundant amino acids. The obtained polypeptide is consistent with the designed molecular structure, and can be used to obtain a variety of active polypeptides in large quantities and at low cost.

The invention relates to the field of gene detection, in particular to a trace short tandem repeat sequence detection method, which comprises the following steps: separating stem cells from a biological sample, combining the stem cells into a cell sample, cracking the cell sample, carrying out polymerase chain reaction and electrophoresis detection, and analyzing obtained data. When the method is used for trace short tandem repeat detection, a high-accuracy sequencing result can be obtained with a small sample size, and detection and analysis of trace samples are facilitated.

The invention provides a chromosome outer circular DNA analysis method and device based on long-read-long sequencing data, a terminal, a medium and a product. After the long-read-long sequencing data to be analyzed is obtained and a reference genome database is established, tandem repeat detection and first comparison analysis are sequentially carried out on the long-read-long sequencing data; performing first comparison analysis and third comparison analysis on unrecognized data in the long-read-long sequencing data to respectively obtain a second chromosome outer circular DNA analysis result and a third chromosome outer circular DNA analysis result, and finally merging the outer circular DNA analysis results of the chromosomes to obtain a first chromosome outer circular DNA analysis result. A final chromosome outer circular DNA analysis result is obtained. Through three times of comparative analysis, the accuracy of circular DNA analysis is improved, and the utilization rate of long reading length data is improved.

The invention belongs to the technical field of bioengineering and polypeptide preparation, and particularly relates to a method for efficiently preparing GHK tripeptide by using recombinant tandem repeat GHK oligopeptide. More specifically, a high-purity GHK tripeptide product is obtained by constructing a recombinant expression vector containing a multi-copy GHK tandem repeat sequence, efficiently expressing a recombinant tandem protein in escherichia coli, and then performing salting-out-isoelectric point-re-salting-out precipitation purification and enzyme digestion release.

The application belongs to the technical field of genetic engineering, and specifically provides a recombinant human-like collagen polypeptide, a preparation method and application thereof. The recombinant human-like collagen polypeptide comprises a tandem repeat sequence at an N terminal and a C terminal sequence; the repeat sequence is shown as SEQ ID NO. 1; and the C terminal sequence is shown as SEQ ID NO. 2. The recombinant human-like collagen polypeptide provided in the application can correctly express active collagen with a triple helix structure in a prokaryotic expression system, greatly reducing the production cost of active human collagen and improving the production efficiency. The prepared recombinant human-like collagen polypeptide has better water retention capacity and anti-degradation capacity than natural human type I collagen, and shows a cell migration promoting effect superior to that of a commercial collagen product in a cell scratch experiment.