34results about How to "Improved genetic traits" patented technology

The invention discloses an application of an MYB28 protein and an encoding gene thereof in regulation and control of tolerance of plants on ABA. The application provided by the invention is an application of the protein as shown in a sequence 3 in the following aspects: (a1) regulation and control of the tolerance of plants on ABA; (a2) breeding of a plant variety which is lowered or increased in the tolerance on ABA; (a3) regulation and control of vivipary resistance of plants; and (a4) breeding of a plant variety which is increased or lowered in the vivipary resistance. The MYB28 protein can be applied to obtaining of MYB28 low-expressed and herbicide-resistant transgenic crops employing plant hormone ABA as a selective herbicide through genetic engineering means, so as to achieve selective weeding; the germination rate of plant seeds can also be controlled by adjusting the expression level of the MYB28; MYB28 gene high-expressed and vivipary-resistant transgenic crops can be obtained through the genetic engineering means; the application conforms to the requirements of sustainable agriculture development, and has important practical value and market prospects in the aspects such as development of green, environment-friendly and pollution-free weeding methods and improvement of eating quality and storage quality of seeds.

The invention discloses application of OTS1 protein and coding genes thereof to control over ABA tolerance of a plant. According to the application, the protein shown as the sequence 3 is applied to: (a1) control over the ABA tolerance of the plant; (a2) breeding of a plant variety with improved or reduced ABA tolerance; (a3) control over vivipary resistance of the plant; (a4) breeding of a plant variety with improved or reduced vivipary resistance. The plant hormone ABA can be used as selective herbicide, transgenic crops with low OTS1 expression and herbicide resistance can be obtained by means of genetic engineering, and selective weeding is achieved. The germination rate of plant seeds can be controlled by regulating the expression level of OTS1, and transgenic crops with high expression of OTS1 genes and vivipary resistance can be obtained by means of genetic engineering. The application meets the development demand for sustainable agriculture and has significant practical value and market prospect in exploiting green, environment-friendly and pollution-free weeding methods and other aspects.

The invention belongs to the technical field of plant tissue and cell culture, and particularly relates to a preparing method of carnation leaf protoplast. With a carnation tissue culture seedling asthe test material, an efficient protoplast preparing method for purification through a low-speed oscillation enzymolysis-sedimentation method is established. The method is convenient to operate and can be implemented all year round, the yield of the protoplast subjected to low-speed oscillation enzymolysis separation and density gradient purification is high and can reach 183*105 p / gFW, the cell integrity is high, and it is ensured that the activity is 75% or above. By means of the method, a foundation is laid for the research on the aspects of gene function authentication, sub-cellular localization, protein interaction and the like of a carnation leaf instantaneous converting system.

The invention discloses a method for improving the tolerance of a plant to droughts through down-regulation of expression of an eIFiso4G1 gene and an eIFiso4G2 gene. Application of the method in inhibiting expression substances of a specific gene A and a specific gene B in the plant comprises regulation and control over drought tolerance of the plant and seed selection for plant varieties of the improved drought tolerance. The specific gene A is a gene for encoding eIFiso4G1 protein; the specific gene B is a gene for encoding eIFiso4G2 protein. The inventor discovers that through knockout of the eIFiso4G1 gene and the eIFiso4G2 gene, the tolerance of the plant to droughts can be significantly improved. The method accords with the demands of the sustainable agricultural development and hasimportant practical value and an important market prospect in the aspects of studying the drought-tolerance molecule mechanism of the plant, improving the hereditary characters of the plant, improvingthe stress resistance of the plant to natural climatic disasters and bad soil environments, cultivating new efficient drought-tolerance varieties and the like.

The invention discloses a 4-terpilenol-type melaleuca alternifolia tissue culture subculture multiplication method. The method comprises the following steps: selecting a superior individual plant of 4-terpilenol-type melaleuca alternifolia to stump or cut annularly for promoting sprouting, collecting sprouting branches, and culturing through specially designed early-generation conductive culture medium and subculture multiplication culture medium in sequence. Experiments show that, by applying the method, the propagation coefficient of bud organs can be improved, the subculture multiplication period of the bud organs can be shortened and the number of effective buds can be increased, so that the production cost of tissue culture industrialized seedling of the 4-terpilenol-type melaleuca alternifolia is reduced; the superior individual plant of the 4-terpilenol-type melaleuca alternifolia is adopted as an explant, and a tissue culture seedling of the explant remains excellent genetic traits of a female parent, so that improved variety system and clone of the melaleuca alternifolia are achieved; the biomass and oil production per unit area can be increased, and the economic benefit and the ecological benefit of melaleuca alternifolia forest stand are improved.

The invention discloses a method for improving NaCl tolerance of plants by reducing PAB2 and PAB8. The method specifically provides any application of protein 1 and protein 2 which serve as targets as follows: a1) salt tolerance of the plants is improved; a2) plant varieties with salt tolerance improved are selected and bred, wherein the protein 1 is PAB2 protein, and the protein 2 is PAB8 protein. Research finds that NaCl tolerance of gene double knockout mutants of PAB2 and PAB8 obtained by an arabidopsis biological research center is enhanced. Accordingly, PAB2 and PAB8 can be used for adjusting the NaCl tolerance of the plants. Besides, genetically modified crops low in PAB2 and PAB8 expression and tolerant to salt can be obtained trough a genetic engineering means (RNAi technology). The method meets the requirement for sustainable agricultural development and has important practical value and market prospects in the aspects of research of plant NaCl tolerance molecular mechanism, improvement of genetic characteristics, culture of efficient salt-tolerant new varieties and the like.

The invention discloses application of eIFiso4G1 protein to regulation and control of the drought resistance of plants. The application required for protecting substances used for inhibiting specificgene expression in the plants comprises the following steps of regulating and controlling the drought resistance of the plants; breeding plant varieties with improved drought resistance. The inventionalso provides a method for preparing transgenic plants. The method comprises the following steps that the specific gene expression in receiver plants is inhibited to obtain the transgenic plants; compared with the receiver plants, the drought resistance of the transgenic plants is improved. A specific gene is a gene encoding the eIFiso4G1 protein. The inventor finds that the drought tolerance ofthe plants can be obviously enhanced by knocking out an eIFiso4G1 gene. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of studying the plant drought resistant molecular mechanism; improving the hereditary character, improving the stress resistance of the plants on natural climatic disasters and poor soil environment, culturing new efficient drought resistant varieties and the like.

The invention discloses a Chinese wild East China grape Baihe-35-1 calcium-dependent protein kinase gene VpCDPK13 and an application thereof. The invention relates to the field of gene engineering, inparticular to the field of grape disease-resistant breeding. The calcium-dependent protein kinase gene VpCDPK13 is separated from the disease-resistant Chinese wild East China grape Baihe-35-1 plantgenome through a gene cloning technology, and is transferred into a powdery mildew susceptible grape plant to obtain a powdery mildew resistant transgenic grape plant, so that the powdery mildew resistance of grapes is improved.

The invention discloses an application of eIFiso4G1 protein in regulating and controlling the tolerance of plants to ABA. The invention discloses application of a substance for inhibiting expression of a specific gene in a plant, which comprises the following steps: regulating and controlling the tolerance of the plant to abscisic acid; breeding a plant variety with reduced tolerance to the abscisic acid; regulating and controlling the embryo sprouting resistance of the plant; breeding a plant variety with increased embryo sprouting resistance, wherein the specific gene is a gene encoding theeIFiso4G1 protein. According to the application, the eIFiso4G1 protein is an important negative regulator in ABA signal transduction, and the resistance of plants to ABA can be obviously reduced by knocking out the eIFiso4G1 gene. The application meets the requirements of sustainable agricultural development, and has important practical value and market prospect in the aspects of cultivating high-efficiency ABA-tolerant and stress-resistant new varieties and the like.

The invention discloses application of eIFiso4G1 protein to regulation and control of the drought resistance of plants. The application required for protecting substances used for inhibiting specificgene expression in the plants comprises the following steps of regulating and controlling the drought resistance of the plants; breeding plant varieties with improved drought resistance. The inventionalso provides a method for preparing transgenic plants. The method comprises the following steps that the specific gene expression in receiver plants is inhibited to obtain the transgenic plants; compared with the receiver plants, the drought resistance of the transgenic plants is improved. A specific gene is a gene encoding the eIFiso4G1 protein. The inventor finds that the drought tolerance ofthe plants can be obviously enhanced by knocking out an eIFiso4G1 gene. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of studying the plant drought resistant molecular mechanism; improving the hereditary character, improving the stress resistance of the plants on natural climatic disasters and poor soil environment, culturing new efficient drought resistant varieties and the like.

The invention discloses a method for improving NaCl tolerance of plants by reducing PAB2 and PAB8. The method specifically provides any application of protein 1 and protein 2 which serve as targets as follows: a1) salt tolerance of the plants is improved; a2) plant varieties with salt tolerance improved are selected and bred, wherein the protein 1 is PAB2 protein, and the protein 2 is PAB8 protein. Research finds that NaCl tolerance of gene double knockout mutants of PAB2 and PAB8 obtained by an arabidopsis biological research center is enhanced. Accordingly, PAB2 and PAB8 can be used for adjusting the NaCl tolerance of the plants. Besides, genetically modified crops low in PAB2 and PAB8 expression and tolerant to salt can be obtained trough a genetic engineering means (RNAi technology). The method meets the requirement for sustainable agricultural development and has important practical value and market prospects in the aspects of research of plant NaCl tolerance molecular mechanism, improvement of genetic characteristics, culture of efficient salt-tolerant new varieties and the like.

The invention discloses application of MYB37 protein and coding genes thereof to control of plant seed germination. Specifically, the protein (namely the MYB37 protein) formed by an amino acid sequence shown as the sequence 3 in a sequence table is applied to (a1) improvement in vivipary resistance of a plant or (a2) breeding of a plant variety with improved vivipary resistance. According to the application, the germination rate of plant seeds can be controlled by regulating the expression level of the MYB37, and high-expression vivipary-resistant transgenic crops containing MYB37 genes are obtained by means of gene engineering. The application meets the sustainable agricultural development demand and has important practical value and market prospect for improving eating quality, storage quality and the like of seeds.

The invention discloses application of eIF4G protein to regulation and control of the tolerance of plants on ABA (abscisic acid). The application required for protecting substances used for inhibitingspecific gene expression in the plants comprises the following steps of regulating and controlling the tolerance of the plants on the ABA; breeding plant varieties with reduced tolerance on the ABA;regulating and controlling vivipary resistance of the plants; breeding plant varieties with increased vivipary resistance, wherein a specific gene is a gene encoding the eIF4G protein. The conditionsthat the eIF4G protein is an important negative regulator in ABA signal conduction, and the tolerance of the plants on the ABA can be obviously reduced by knocking out the eIF4G gene are found. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of culturing new efficient ABA and adversity stress resistant varieties and the like.

The invention discloses a method for improving tolerance of plants for NaCl by reducing the content of PAB2 and PAB4. The method specifically provides an application of protein 1 and protein 2 serving as targets to any one of the following conditionsthat a1, salt tolerance of the plants is improved; a2, plant varieties with improved salt resistance are bred. Protein 1 is PAB2, and protein 2 is PAB4. It is found through the research that tolerance of PAB2 and PAB4 gene double knockout mutants obtained in the arabidopsis thaliana bioresearch center to NaCl is enhanced; thus, the tolerance of the plants to NaCl can be adjusted through PAB2 and PAB4; in addition, PAB2 and PAB4 low-expression and salt-resistant transgenic crops can be obtained through the gene engineering means (PNAi technology). The method meets the sustainable agriculture development demand, and high practical value and market prospects are achieved on the aspects of researching the molecular mechanism for plants to tolerant NaCl, improving hereditary characters, cultivating efficient salt-resistant new varieties and the like.

The invention belongs to the technical field of plant tissue and cell culture, and in particular relates to a method for preparing carnation leaf protoplasts. Taking carnation seedlings as experimental materials, a high-efficiency protoplast preparation system was established by low-speed oscillation enzymatic hydrolysis-sedimentation method. The invention is easy to operate and can be carried out all year round, and the protoplast yield after low-speed shaking enzymatic separation and density gradient purification is high, up to 183×10 5 cells / gFW, the cell integrity rate is high, and the viability is guaranteed to be above 75%. The invention lays a foundation for the study of gene function identification, subcellular localization, protein interaction and the like using the carnation leaf transient transformation system.

The invention provides a method for establishing a lycoris regeneration system. The method comprises the following steps: 1) carrying out sterilization, multiple shoot induction, adventitious bud proliferation and strong bud culture on underground bulbs of lycoris to obtain newborn bulbs; 2) inoculating the newborn bulbs to a callus inducing culture medium to be subjected to dark culture so as to obtain callus tissues of which the diameters are 1.5 to 2.5cm; 3) cutting the callus tissues into clumps of which the diameters are 1 to 2cm and then inoculating to a shoot induction culture medium to differentiate the callus tissues for 15 to 20 d to obtain multiple shoots; and 4) inoculating the multiple shoots to a strong bud rooting culture medium to be subjected to strong bud rooting culture so as to obtain tissue culture seedlings. The multiple shoots induced by the callus tissues are large in number, high in growth speed and capable of greatly improving the lycoris propagation coefficient and further increasing the propagation speed; and in addition, a high-efficient genetic transformation system of the lycoris is expected to be established through the callus tissues to realize genetic improvement on the molecular biology level of the lycoris.

The invention discloses the application of the eIFiso4G1 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G1 protein. The present invention finds that the eIFiso4G1 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G1 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

The invention discloses a preparation method of lily petal protoplasts, which comprises the steps of: (1) taking a lily plant with unopened flower buds and performing dark treatment; (2) taking the inner round petals of the plant, cleaning and disinfecting them; Add enzymolysis solution to the petal material, vacuum extract in the dark, and then stand in the dark for several hours to obtain the enzymolysis solution containing petal protoplasts; (4) The obtained enzymolysis solution is diluted with W5 solution, filtered through a mesh screen, The obtained filtrate was centrifuged at low speed, the supernatant was discarded, the protoplast precipitate was resuspended with W5 solution, the protoplast pellet was resuspended with MMG solution, the protoplast pellet was resuspended, and the supernatant was discarded. The invention overcomes the problem of low separation efficiency of the lily petal protoplast, and effectively establishes a method for preparing the lily petal protoplast.

The invention discloses a method for improving tolerance of plants to NaCl by lowering PAB4 and PAB8. The invention provides application of protein 1 and protein 2 used as targets in improving salt tolerance of plants or selecting and breeding plant varieties with improved salt tolerance; the protein 1 is PAB4 protein, and the protein 2 is PAB8 protein. Researches show that the tolerance of a PAB4 and PAB8 gene double knockout mutant obtained from the arabidopsis thaliana biological research center to NaCl is enhanced; accordingly, the tolerance of plants to NaCl can be adjusted through PAB4 and PAB8. In addition, PAB4 and PAB8 low-expression and salt-tolerance transgenic crops can be obtained through a genetic engineering means (RNAi technology). The method meets the requirements for sustainable agriculture development, and has the important practical value and market prospect in the aspects of researching molecular mechanisms of tolerance of plants to NaCl, improving hereditary characters, breeding efficient salt-tolerance new variety and the like.

The invention discloses an application of an MYB28 protein and an encoding gene thereof in regulation and control of tolerance of plants on ABA. The application provided by the invention is an application of the protein as shown in a sequence 3 in the following aspects: (a1) regulation and control of the tolerance of plants on ABA; (a2) breeding of a plant variety which is lowered or increased in the tolerance on ABA; (a3) regulation and control of vivipary resistance of plants; and (a4) breeding of a plant variety which is increased or lowered in the vivipary resistance. The MYB28 protein can be applied to obtaining of MYB28 low-expressed and herbicide-resistant transgenic crops employing plant hormone ABA as a selective herbicide through genetic engineering means, so as to achieve selective weeding; the germination rate of plant seeds can also be controlled by adjusting the expression level of the MYB28; MYB28 gene high-expressed and vivipary-resistant transgenic crops can be obtained through the genetic engineering means; the application conforms to the requirements of sustainable agriculture development, and has important practical value and market prospects in the aspects such as development of green, environment-friendly and pollution-free weeding methods and improvement of eating quality and storage quality of seeds.

The invention discloses application of eIF4G protein to regulation and control of the tolerance of plants on ABA (abscisic acid). The application required for protecting substances used for inhibitingspecific gene expression in the plants comprises the following steps of regulating and controlling the tolerance of the plants on the ABA; breeding plant varieties with reduced tolerance on the ABA;regulating and controlling vivipary resistance of the plants; breeding plant varieties with increased vivipary resistance, wherein a specific gene is a gene encoding the eIF4G protein. The conditionsthat the eIF4G protein is an important negative regulator in ABA signal conduction, and the tolerance of the plants on the ABA can be obviously reduced by knocking out the eIF4G gene are found. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of culturing new efficient ABA and adversity stress resistant varieties and the like.

The invention discloses a method for improving the tolerance of plants to drought by down-regulating an eIF4G gene and an eIFiso4G1 gene. The invention provides application of an expression substanceused for inhibiting a specific gene A in the plants and an expression substance used for inhibiting a specific gene B in the plants, which comprises regulation of the drought resistance of the plantsand breeding of plant varieties with improved drought resistance. The specific gene A is a gene encoding eIF4G protein. The specific gene B is a gene encoding eIFiso4G1 protein. The inventor finds that the knockout of the eIF4G gene and eIFiso4G1 gene can make the tolerance of the plants to the drought significantly enhanced. The method for improving the tolerance of the plants to the drought by down-regulating the eIF4G gene and the eIFiso4G1 gene meets the requirements of sustainable agricultural development, and has important practical value and market prospects for studying the molecular mechanism of drought tolerance of the plants, improving genetic characteristics, improving the resistance of crops to natural climatic disasters and adverse soil environment, cultivating novel high-efficiency drought-resistant varieties and the like.

The invention discloses a method for improving tolerance of plants to NaCl by lowering PAB4 and PAB8. The invention provides application of protein 1 and protein 2 used as targets in improving salt tolerance of plants or selecting and breeding plant varieties with improved salt tolerance; the protein 1 is PAB4 protein, and the protein 2 is PAB8 protein. Researches show that the tolerance of a PAB4 and PAB8 gene double knockout mutant obtained from the arabidopsis thaliana biological research center to NaCl is enhanced; accordingly, the tolerance of plants to NaCl can be adjusted through PAB4 and PAB8. In addition, PAB4 and PAB8 low-expression and salt-tolerance transgenic crops can be obtained through a genetic engineering means (RNAi technology). The method meets the requirements for sustainable agriculture development, and has the important practical value and market prospect in the aspects of researching molecular mechanisms of tolerance of plants to NaCl, improving hereditary characters, breeding efficient salt-tolerance new variety and the like.

The invention discloses application of eIFiso4G2 protein for regulating tolerance of plants to ABA. The application of the eIFiso4G2 protein for regulating tolerance of the plants to ABA, the eIFiso4G2 protein is used for protecting substances which inhibit expression of specific genes in the plants, regulating the tolerance of the plants to abscisic acid, selectively breeding plant varieties of which the tolerance to the abscisic acid is reduced, regulating vivipary resistance of the plants and selectively breeding plant varieties with increasing vivipary resistance, and the specific genes are genes which encode the eIFiso4G2 protein. According to the application of the eIFiso4G2 protein for regulating tolerance of the plants to ABA, the eIFiso4G2 protein is an important negative regulator in conduction of ABA signals, and by knocking eIFiso4G2 genes out, the tolerance of the plants to the ABA can be obviously reduced. The application of the eIFiso4G2 protein for regulating toleranceof the plants to ABA accords with sustainable agricultural development requirements and has high practical value and market prospects on cultivation of new efficient ABA-tolerating adversity stress varieties.

The invention discloses the application of the eIFiso4G2 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G2 protein. The present invention finds that the eIFiso4G2 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G2 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

The invention discloses a method for improving the tolerance of plants to drought by down-regulating an eIF4G gene and an eIFiso4G1 gene. The invention provides application of an expression substanceused for inhibiting a specific gene A in the plants and an expression substance used for inhibiting a specific gene B in the plants, which comprises regulation of the drought resistance of the plantsand breeding of plant varieties with improved drought resistance. The specific gene A is a gene encoding eIF4G protein. The specific gene B is a gene encoding eIFiso4G1 protein. The inventor finds that the knockout of the eIF4G gene and eIFiso4G1 gene can make the tolerance of the plants to the drought significantly enhanced. The method for improving the tolerance of the plants to the drought by down-regulating the eIF4G gene and the eIFiso4G1 gene meets the requirements of sustainable agricultural development, and has important practical value and market prospects for studying the molecular mechanism of drought tolerance of the plants, improving genetic characteristics, improving the resistance of crops to natural climatic disasters and adverse soil environment, cultivating novel high-efficiency drought-resistant varieties and the like.

The invention discloses a 4-terpineol-type Melaleuca alternifolia tissue culture subculture propagation method. In the method, an excellent individual plant of the 4-terpineol-type Melaleuca alternifolia is selected for pile cutting or circumcision to promote germination. Germination strips were collected and cultured in the specially designed primary induction medium and secondary proliferation medium. Experiment shows, application of the present invention can improve bud organ reproduction coefficient, shortens bud organ subculture propagation cycle, increases effective bud number, thereby reduces the production cost in 4 terpineol type Melaleuca alternifolia tissue culture industrialized seedling cultivation; The explants are collected from 4‑terpineol-type Melaleuca alternifolia excellent individual plants, and its tissue culture seedlings maintain the excellent genetic traits of the female parent, so that the Melaleuca alternifolia seedlings can be improved and cloned, and the unit can be increased The biomass and oil production of the area can be improved, and the economic and ecological benefits of the Melaleuca forest stand can be improved.