34results about How to "Improved genetic traits" patented technology

The invention discloses application of eIFiso4G1 protein to regulation and control of the drought resistance of plants. The application required for protecting substances used for inhibiting specificgene expression in the plants comprises the following steps of regulating and controlling the drought resistance of the plants; breeding plant varieties with improved drought resistance. The inventionalso provides a method for preparing transgenic plants. The method comprises the following steps that the specific gene expression in receiver plants is inhibited to obtain the transgenic plants; compared with the receiver plants, the drought resistance of the transgenic plants is improved. A specific gene is a gene encoding the eIFiso4G1 protein. The inventor finds that the drought tolerance ofthe plants can be obviously enhanced by knocking out an eIFiso4G1 gene. The sustainable agriculture development requirement is conformed; important practical values and market prospects are realized in aspects of studying the plant drought resistant molecular mechanism; improving the hereditary character, improving the stress resistance of the plants on natural climatic disasters and poor soil environment, culturing new efficient drought resistant varieties and the like.

The invention discloses a method for improving NaCl tolerance of plants by reducing PAB2 and PAB8. The method specifically provides any application of protein 1 and protein 2 which serve as targets as follows: a1) salt tolerance of the plants is improved; a2) plant varieties with salt tolerance improved are selected and bred, wherein the protein 1 is PAB2 protein, and the protein 2 is PAB8 protein. Research finds that NaCl tolerance of gene double knockout mutants of PAB2 and PAB8 obtained by an arabidopsis biological research center is enhanced. Accordingly, PAB2 and PAB8 can be used for adjusting the NaCl tolerance of the plants. Besides, genetically modified crops low in PAB2 and PAB8 expression and tolerant to salt can be obtained trough a genetic engineering means (RNAi technology). The method meets the requirement for sustainable agricultural development and has important practical value and market prospects in the aspects of research of plant NaCl tolerance molecular mechanism, improvement of genetic characteristics, culture of efficient salt-tolerant new varieties and the like.

The invention discloses a method for improving tolerance of plants for NaCl by reducing the content of PAB2 and PAB4. The method specifically provides an application of protein 1 and protein 2 serving as targets to any one of the following conditionsthat a1, salt tolerance of the plants is improved; a2, plant varieties with improved salt resistance are bred. Protein 1 is PAB2, and protein 2 is PAB4. It is found through the research that tolerance of PAB2 and PAB4 gene double knockout mutants obtained in the arabidopsis thaliana bioresearch center to NaCl is enhanced; thus, the tolerance of the plants to NaCl can be adjusted through PAB2 and PAB4; in addition, PAB2 and PAB4 low-expression and salt-resistant transgenic crops can be obtained through the gene engineering means (PNAi technology). The method meets the sustainable agriculture development demand, and high practical value and market prospects are achieved on the aspects of researching the molecular mechanism for plants to tolerant NaCl, improving hereditary characters, cultivating efficient salt-resistant new varieties and the like.

The invention belongs to the technical field of plant tissue and cell culture, and in particular relates to a method for preparing carnation leaf protoplasts. Taking carnation seedlings as experimental materials, a high-efficiency protoplast preparation system was established by low-speed oscillation enzymatic hydrolysis-sedimentation method. The invention is easy to operate and can be carried out all year round, and the protoplast yield after low-speed shaking enzymatic separation and density gradient purification is high, up to 183×10 5 cells / gFW, the cell integrity rate is high, and the viability is guaranteed to be above 75%. The invention lays a foundation for the study of gene function identification, subcellular localization, protein interaction and the like using the carnation leaf transient transformation system.

The invention provides a method for establishing a lycoris regeneration system. The method comprises the following steps: 1) carrying out sterilization, multiple shoot induction, adventitious bud proliferation and strong bud culture on underground bulbs of lycoris to obtain newborn bulbs; 2) inoculating the newborn bulbs to a callus inducing culture medium to be subjected to dark culture so as to obtain callus tissues of which the diameters are 1.5 to 2.5cm; 3) cutting the callus tissues into clumps of which the diameters are 1 to 2cm and then inoculating to a shoot induction culture medium to differentiate the callus tissues for 15 to 20 d to obtain multiple shoots; and 4) inoculating the multiple shoots to a strong bud rooting culture medium to be subjected to strong bud rooting culture so as to obtain tissue culture seedlings. The multiple shoots induced by the callus tissues are large in number, high in growth speed and capable of greatly improving the lycoris propagation coefficient and further increasing the propagation speed; and in addition, a high-efficient genetic transformation system of the lycoris is expected to be established through the callus tissues to realize genetic improvement on the molecular biology level of the lycoris.

The invention discloses the application of the eIFiso4G1 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G1 protein. The present invention finds that the eIFiso4G1 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G1 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

The invention discloses application of a CPN20 protein and a coding gene thereof in regulating the ABA (abscisic acid) tolerance of a plant. The application provided by the invention is particularly application of a protein composed of an amino acid sequence as shown in Sequence 3 in a sequence table in a1) or a2) below: a1) regulating the ABA tolerance of a plant; and a2) breeding ABA tolerance improved plant species. The invention can be used for using phytohormone ABA as a selective weedicide, the selectivity depends on the ABA sensitivity of the plant, and the CPN20 gene is used to regulate the ABA sensitivity or tolerance of the plant, thus obtaining CPN20 gene highly-expressed anti-weedicide transgenic crops and further realizing selective weed control. The invention meets the sustainable agricultural development requirements, and has important practical values and market prospects in the aspects of improving hereditary characters, developing green environment-friendly nuisance-free weed control methods and approaches and the like.

The invention discloses a method for preparing lily mesophyll protoplasts, which comprises the steps of: (1) taking lily plants with unopened flower buds, and performing dark treatment; (2) taking leaves of the plants, cleaning and disinfecting them; (3) feeding the mesophyll materials Add enzymolysis solution in the dark place, vacuum extraction in the dark, and then stand in the dark for several hours to obtain the enzymolysis solution containing mesophyll protoplasts; (4) Dilute the obtained enzymolysis solution with W5 solution, filter through a mesh sieve, and obtain the filtrate After low-speed centrifugation, discard the supernatant, resuspend the protoplast pellet with W5 solution, centrifuge at low speed, discard the supernatant, and repeat washing once; resuspend the protoplast pellet with MMG solution. The invention overcomes the problem of low separation efficiency of the lily mesophyll protoplast, and effectively establishes a method for preparing the lily mesophyll protoplast.

The invention discloses a gene for regulating plant seed germination and seedling growth, its encoded protein and application. The inventors of the present invention identified and obtained the NtCIPK23 gene from the common tobacco (Nicotiana tabacum) cultivar Zhongyan 100, the nucleotide sequence of the NtCIPK23 gene is shown in SEQ ID NO.1, and proved by transgenic technology that overexpressing NtCIPK23 can Accelerate the germination rate of tobacco seeds and promote the growth of seedlings, while knocking out the gene will delay the germination rate of seedlings and inhibit the growth of seedlings. By using the present invention, the germination rate and uniformity of plant seeds can be controlled by adjusting the expression level of CIPK23, and the transgenic crops with moderate expression of CIPK23 gene and uniform emergence rate can be obtained by means of genetic engineering. The invention meets the requirements of sustainable agricultural development, and has important practical value and market prospect for cultivating economic crops that grow early, grow fast, and emerge neatly.

The invention discloses a method for improving tolerance of plants to NaCl by lowering PAB4 and PAB8. The invention provides application of protein 1 and protein 2 used as targets in improving salt tolerance of plants or selecting and breeding plant varieties with improved salt tolerance; the protein 1 is PAB4 protein, and the protein 2 is PAB8 protein. Researches show that the tolerance of a PAB4 and PAB8 gene double knockout mutant obtained from the arabidopsis thaliana biological research center to NaCl is enhanced; accordingly, the tolerance of plants to NaCl can be adjusted through PAB4 and PAB8. In addition, PAB4 and PAB8 low-expression and salt-tolerance transgenic crops can be obtained through a genetic engineering means (RNAi technology). The method meets the requirements for sustainable agriculture development, and has the important practical value and market prospect in the aspects of researching molecular mechanisms of tolerance of plants to NaCl, improving hereditary characters, breeding efficient salt-tolerance new variety and the like.

The invention discloses the application of the eIFiso4G2 protein in regulating the tolerance of plants to ABA. The present invention claims to protect the application of substances used to inhibit the expression of specific genes in plants: regulating the tolerance of plants to abscisic acid; breeding plant varieties with reduced tolerance to abscisic acid; regulating the embryonic resistance of plants; breeding A plant variety with increased fetal germination resistance; the specific gene is the gene encoding eIFiso4G2 protein. The present invention finds that the eIFiso4G2 protein is an important negative regulator in ABA signal transduction, and knocking out the eIFiso4G2 gene can significantly reduce the tolerance of plants to ABA. The invention meets the requirement of sustainable agricultural development, and has important practical value and market prospect for cultivating new varieties with high efficiency and tolerance to ABA and adversity stress.

The invention discloses a 4-terpineol-type Melaleuca alternifolia tissue culture subculture propagation method. In the method, an excellent individual plant of the 4-terpineol-type Melaleuca alternifolia is selected for pile cutting or circumcision to promote germination. Germination strips were collected and cultured in the specially designed primary induction medium and secondary proliferation medium. Experiment shows, application of the present invention can improve bud organ reproduction coefficient, shortens bud organ subculture propagation cycle, increases effective bud number, thereby reduces the production cost in 4 terpineol type Melaleuca alternifolia tissue culture industrialized seedling cultivation; The explants are collected from 4‑terpineol-type Melaleuca alternifolia excellent individual plants, and its tissue culture seedlings maintain the excellent genetic traits of the female parent, so that the Melaleuca alternifolia seedlings can be improved and cloned, and the unit can be increased The biomass and oil production of the area can be improved, and the economic and ecological benefits of the Melaleuca forest stand can be improved.