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Application of eIFiso4G1 protein in regulating and controlling tolerance of plants to ABA

A tolerance, plant technology, applied in applications, plant peptides, plant products, etc., can solve the problems of consumption of nutrients and storage substances, edible quality of seeds, deterioration of storage quality, loss of agricultural production, etc.

Inactive Publication Date: 2019-07-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fetal germination consumes part of its nutrients and storage substances, resulting in a decline in the edible quality and storage quality of the seeds, causing greater losses to agricultural production

Method used

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  • Application of eIFiso4G1 protein in regulating and controlling tolerance of plants to ABA
  • Application of eIFiso4G1 protein in regulating and controlling tolerance of plants to ABA
  • Application of eIFiso4G1 protein in regulating and controlling tolerance of plants to ABA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the acquisition and identification of mutants

[0065] 1. The acquisition of mutant ei4g1-1

[0066] Seeds of Arabidopsis thaliana with the number SALK_098730 (the background is Arabidopsis ecotype Columbia, a T-DNA insertion mutant) were purchased from the Arabidopsis Biological Research Center and grown into plants. The genomic DNA of the seedling stage plants was extracted, and PCR identification was carried out with the primer pair consisting of ei4g1-1 LP and ei4g1-1 RP and the primer pair consisting of LBb1.3 and ei4g1-1 RP, respectively. If the amplified product is not detected by the primer pair composed of ei4g1-1 LP and ei4g1-1 RP, and the amplified product can be detected by the primer pair composed of LBb1.3 and ei4g1-1 RP, it proves that the plant is two Chromosomally identical homozygous mutants. Homozygous mutants were sequenced for verification.

[0067] ei4g1-1 LP: 5'-TCAAGGGCAAACATATCATCC-3';

[0068] ei4g1-1 RP: 5'-TTTTGACTTCACGTTTCC...

Embodiment 2

[0092] Embodiment 2, the tolerance of plant to ABA

[0093] 1. Seed Germination Experiment

[0094] ABA plays an important regulatory role in promoting seed dormancy and inhibiting seed germination. Genetic studies have shown that processes such as chromatin remodeling, histone methylation, and deacetylation that occur during seed germination are all regulated by ABA. With the increase of exogenous ABA concentration, the germination rate of wild-type Arabidopsis seeds will gradually decrease. Statistical seed germination experiment is one of the important methods to study ABA signal transduction.

[0095] The tested seeds are: seeds of Arabidopsis thaliana ecotype Columbia, seeds of mutant ei4g1-1.

[0096] The first group: sow the tested seeds on the MS medium plate, stratify at 4°C for 3 days, then transfer to the light incubator for cultivation, and culture in the light incubator for 36 hours, 48 ​​hours or 72 hours, then count Seed germination rate.

[0097] The secon...

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Abstract

The invention discloses an application of eIFiso4G1 protein in regulating and controlling the tolerance of plants to ABA. The invention discloses application of a substance for inhibiting expression of a specific gene in a plant, which comprises the following steps: regulating and controlling the tolerance of the plant to abscisic acid; breeding a plant variety with reduced tolerance to the abscisic acid; regulating and controlling the embryo sprouting resistance of the plant; breeding a plant variety with increased embryo sprouting resistance, wherein the specific gene is a gene encoding theeIFiso4G1 protein. According to the application, the eIFiso4G1 protein is an important negative regulator in ABA signal transduction, and the resistance of plants to ABA can be obviously reduced by knocking out the eIFiso4G1 gene. The application meets the requirements of sustainable agricultural development, and has important practical value and market prospect in the aspects of cultivating high-efficiency ABA-tolerant and stress-resistant new varieties and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of eIFiso4G1 protein in regulating plant tolerance to ABA. Background technique [0002] Abscisic acid (ABA) is one of the important hormones in plants. It plays an important role in all stages of plant growth and development, including seed dormancy, germination, seedling growth, stomatal movement, and transition from vegetative growth to reproductive growth. Wait for the process. At the same time, ABA plays an important role in the response of plants to various external adversity stresses, such as abiotic stresses such as drought, low temperature, high temperature, salt, mechanical damage, and biotic stresses such as pests and diseases. Therefore, the elucidation of the molecular mechanism of the plant ABA signal transduction pathway is of great significance for regulating plant growth, improving crop quality, improving genetic characteristics, and promo...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K19/00C12N15/82A01H5/00
CPCC07K14/415C07K2319/00C12N15/8293
Inventor 张大鹏毕超马宇
Owner TSINGHUA UNIV
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