The invention discloses a fruit and vegetable fresh keeping carton with high ventilation, moisture preservation and bacteria resistance. The invention further discloses a preparation method of the fruit and vegetable fresh keeping carton with high ventilation, moisture preservation and bacteria resistance; after scirpoides holoschoenus is mixed with wood slag and bamboo slag for stewing, calcite powder, acadizcolite powder, mordenite powder, nanometer silicon dioxide and nanometer titanium dioxide are added and uniformly stirred for stewing, mixing, papermaking, drying, polishing, paper rolling, cropping, sorting and packing to obtain a paper material; linear low-density polyethylene, polystyrene, a preservative, an antifogging agent, an antibacterial agent, white carbon black, silica gel, Dagu stones, ceramic particles and polyolefin are mixed and homogenized, are heated, shorn, extruded and pelletized by a double-screw extruder, and are blown by a single-screw film blowing machine to obtain a material e; bonding layers are uniformly coated at the periphery of the paper material; and the material e is coated at one sides, far from the paper layer, of the bonding layers. The fruit and vegetable fresh keeping carton has high ventilation and high moisture preservation, and has the antibacterial effect.