The invention relates to an environment-friendly fruit and vegetable cold storage and preservation method, which includes: (1) sterilization treatment; (2) color protection treatment: fruits and vegetables are then precooled in a freezer under 0 DEG C to 5 DEG C for 1 to 3 hours, and are put into a washing basket after being taken out, water which is at 45 DEG C to 50 DEG C is first used for sprinkling for 5 to 10 minutes, water which is at 3 DEG C to 5 DEG C is then used for sprinkling for 5 to 10 minutes, the fruits and the vegetables are taken out and then are immersed for 15 to 20 minutesin water which is at 32 DEG C to 35 DEG C, and finally, the fruits and the vegetables are immersed for 22 to 25 minutes in water which is at 1 DEG C to 5 DEG C; (3) spraying of a preservative; (4) low-temperature storage. The pollution-free greenhouse fruit and vegetable cold storage and preservation method has the advantages of realizing high preservation speed and an excellent melon, fruit and vegetable preservation function, inhibiting the metabolism of the fruits and the vegetables and the breeding and spreading of pathogenic bacteria, delaying the after-ripening and senescence of melons,the fruits and the vegetables, preventing rotting and deterioration, decreasing the browning rate and the rotting rate, and achieving the effects of preservation, peculiar smell elimination, browningresisting, storage time prolongation and outward transportation range broadening.