71results about How to "Prevent browning" patented technology

The invention discloses a Zhejiang camellia japonica clone tissue culture and rapid propagation method. A Zhejiang camellia japonica clone tissue culture and rapid propagation technical system is established by taking a non-lignified tender stem as an explant, sterilizing and then performing primary induction, subculture multiplication, strong bud culture and rooting culture. The explants are soaked before primary inoculation, and vitamin C and polyvinylpyrrolidone with certain concentration are added into a primary induction culture medium, so that browning of the explants can be effectively inhibited; hormone regulation and control and nutrient addition are combined, so that the primary induction rate reaches up to 95.3%, the multiplication coefficient reaches about 6.8, the rooting rate reaches 83.5%, efficient and rapid propagation can be realized, and the method is not limited by factors such as seasons, climate and regions. The rapid propagation offspring obtained by the system can maintain all excellent characters of a stock plant, and the strong bud obtained in the process can be used as a scion for grafting propagation, so that a technical support is provided for protection, development and utilization of rare germplasm resources and excellent clone of the Zhejiang red camellia.

The invention discloses a treatment method for relieving respiratory climacteric fruit and vegetable oxidation browning, and belongs to the technical field of fruit and vegetable storage and fresh-keeping processing. The method comprises the following steps: soaking treatment equipment and fruits and vegetables with NaClO or 75% ethanol solution, washing with deionized water, draining, and cutting into fresh-cut fruit and vegetable slices; soaking the pretreated fresh-cut fruit and vegetable slices in a 2-50 [mu] mol / L theanine solution, taking out the fresh-cut fruit and vegetable slices after the fresh-cut fruit and vegetable slices are completely soaked, and removing moisture on the surfaces of the fresh-cut fruit and vegetable slices; and storing the fresh-cut fruit and vegetable slices, wherein the sealed storage temperature is 0.5-8 DEG C. The treatment method can effectively slow down browning of the fresh-cut fruits and vegetables, is pollution-free to the environment, safe and sanitary, can improve the edible flavor of the fresh-cut fruits, and has the advantages of being green, environment-friendly, high in quality and low in price; the method not only can be used for relieving oxidation browning of fresh-cut apples, pears and yellow peaches, but also can be applied to browning and preservation of other breath climacteric fruits and vegetables, weight loss is reduced, and the method has good economic value.

The invention relates to nutritional wet noodles rich in mixed beans and a preparation method of the nutritional wet noodles. The nutritional wet noodles comprise the following raw materials in parts by weight: 80-100 parts of flour, 5-10 parts of red beans, 5-10 parts of kidney beans, 5-10 parts of mung beans, 5-10 parts of peas, 5-10 parts of broad beans, 2-5 parts of starch acetate and 10-15 parts of an additive, the additive is in a micro-capsule shape, an internal profile is a composite extract, the composite extract comprises a scutellaria baicalensis extract and lecithin, the scutellaria baicalensis extract has good blood fat and blood pressure reducing effects and has a good dietary therapy effect on people suffering from hypertension, hyperlipidemia and hyperglycemia, and meanwhile the lecithin can reduce cholesterol in serum, so that the blood fat and the blood pressure can be reduced. The wall material is prepared by blending an intermediate 6, deionized water and a gelatin solution, and a coating solution contains chitosan and phosphate structures, so that browning of wet noodles can be effectively inhibited, the product quality is ensured, and the wet noodles can be placed for a longer time.

The invention discloses an apple rootstock tissue culture seedling raising method, and relates to the technical field of tissue culture. The method comprises the following steps: (1) washing annual robust branches with tender shoots in a dormancy period with running water, soaking with soapy water, cleaning, obliquely shearing into stem segments, soaking with alcohol, washing, sterilizing, washing, absorbing moisture, and shearing off wounds to obtain pretreated explants; (2) inoculating the pretreated explant into a differential culture medium, and culturing to obtain an adventitious bud; (3) transferring the adventitious buds into a proliferation culture medium, and performing dark culture and light culture to obtain adventitious bud clusters; (4) inoculating the adventitious bud clusters into a rooting culture medium, and culturing to obtain tissue culture seedlings; and (5) hardening the tissue culture seedlings, washing off the culture medium, disinfecting, putting into a culture medium, culturing to obtain apple seedlings, and transplanting to outdoor soil. According to the method, the seedlings capable of being transplanted outdoors can be rapidly obtained, and the problems of infectious microbe pollution, low survival rate, poor rooting effect and the like in existing tissue culture seedling culture are effectively solved.

According to the method for prolonging the fresh-keeping period of the fruits by using the controlled atmosphere fresh-keeping box, the controlled atmosphere fresh-keeping box is prepared by improving the sealing performance of a common fresh-keeping box and covering a certain area of the common fresh-keeping box with a film, and the fruits are put into the box to be stored. When the area of the covering film is 20%, the lotus seeds are stored in a box, and 1-methylcyclopropene can be added; when the area of the covering film is 34.8%, the longan is placed in a box to be stored. Compared with the control, the modified atmosphere preservation box treatment can reduce the weight loss rate and browning degree of the lotus seeds and maintain the contents of Vc, amylose, total phenols and soluble solids; compared with a control group, the modified atmosphere preservation box treatment can inhibit browning of longan peel, delay reduction of soluble solids, acidity and vitamin C, inhibit increase of malondialdehyde content, reduce peroxidase activity and improve catalase activity and glutathione content. According to the technology, the shelf life of the fruits can be effectively prolonged, and the problems that an existing fresh-keeping method is tedious and short in fresh-keeping period are solved.

The invention relates to the technical field of browning inhibition of fermented rose wine, in particular to a composite browning inhibitor for fermented rose wine and application of the composite browning inhibitor. The composite browning inhibitor provided by the invention is a browning inhibitor especially suitable for the fermented rose wine, can effectively inhibit the browning of the fermented rose wine, and can effectively improve the sensory quality of the fermented rose wine. The composite browning inhibitor is prepared from the following components: calcium chloride, glutathione, EDTA-Na2 (Ethylene Diamine Tetraacetic Acid) and D-sodium erythorbate, wherein the mass ratio of the calcium chloride to the glutathione to the EDTA-Na2 to the D-sodium erythorbate is (1.3 to 2.0) to (0.5 to 1.1) to (0.02 to 0.06) to (0.12 to 0.19).

The invention discloses an apple rootstock tissue culture seedling raising method, and relates to the technical field of tissue culture. The method comprises the following steps: (1) washing annual robust branches with tender shoots in a dormancy period with running water, soaking with soapy water, cleaning, obliquely shearing into stem segments, soaking with alcohol, washing, sterilizing, washing, absorbing moisture, and shearing off wounds to obtain pretreated explants; (2) inoculating the pretreated explant into a differential culture medium, and culturing to obtain an adventitious bud; (3) transferring the adventitious buds into a proliferation culture medium, and performing dark culture and light culture to obtain adventitious bud clusters; (4) inoculating the adventitious bud clusters into a rooting culture medium, and culturing to obtain tissue culture seedlings; and (5) hardening the tissue culture seedlings, washing off the culture medium, disinfecting, putting into a culture medium, culturing to obtain apple seedlings, and transplanting to outdoor soil. According to the method, the seedlings capable of being transplanted outdoors can be rapidly obtained, and the problems of infectious microbe pollution, low survival rate, poor rooting effect and the like in existing tissue culture seedling culture are effectively solved.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a genetic transformation method of perennial elymus nutans. According to the genetic transformation method of elymus nutans, mature embryos serve as explants for inducing callus formation, the method has the advantages that material obtaining is convenient, operation is simple and easy, the supply problem of explant material obtaining is solved, and meanwhile the physiological state of the mature embryos is consistent, so that the repeatability of experiments is good; in addition, the infection liquid contains the acetosyringone, and the acetosyringone (AS, Acetosyringone) can promote the growth of agrobacterium tumefaciens and enhance the activity of the agrobacterium tumefaciens, so that the conversion efficiency is improved; the browning phenomenon of the calluses is effectively inhibited through sterilization treatment, the normal growth of the calluses can be recovered, the transgenic elymus nutans plants are obtained through the technical scheme, and the research of researchers on the gene functions of the elymus nutans can be greatly promoted.

The invention provides a method for delaying post-harvest physiological deterioration of cassava and reducing superoxide anion level of cassava through nickel sulfate treatment, and belongs to the technical field of post-harvest preservation of cassava. According to the method, cassava is treated through nickel sulfate contact, the superoxide anion level of cassava is reduced, and post-harvest physiological deterioration of cassava is delayed; therefore, physiological browning and deterioration of the cassava after picking are effectively prevented, and good storage quality of the cassava can be maintained. The method is high in practicability and simple and convenient to operate, can effectively prolong the storage time of the cassava, and has good development and application values in the links of storage, transportation, industrial production and the like of the cassava as an industrial raw material.

This invention relates to the field of food preservation technology and discloses a liquid preservative for preserving fresh walnut kernels. The preservative comprises a natural preservative, a solubilizing and stabilizing agent, and a solvent. This liquid preservative uses natural phloretin as the preservative component, combined with Tween 80 as a solubilizing and stabilizing agent and ethanol as a solvent. This effectively solves the problems of poor water and oil solubility and inadequate stability of phloretin, allowing it to be stably dispersed in the preservative system and fully exert its antioxidant and antimicrobial effects. Furthermore, the preparation method is simple and easy to implement, and the production process is safe and controllable. It is convenient to use; the preservation of fresh walnut kernels can be achieved through dilution and soaking. The diluted solution can be reused, reducing usage costs. Simultaneously, it can inhibit bacterial growth during the storage of fresh walnut kernels, delay oxidative deterioration, maintain their good appearance and quality, extend shelf life, and meet the market demand for high-quality fresh walnut kernels.

The utility model relates to the technical field of sweet potato peeling, and discloses a sweet potato selecting and peeling device which comprises a peeling box, the top of the peeling box is connected with a box cover through a hinge, a motor is installed at the bottom of the peeling box, and the output end of the motor is fixedly connected with a rotating shaft. The other end of the rotating shaft extends into the peeling box and is fixedly connected with a centrifugal disc, the top of the centrifugal disc is fixedly connected with a frosted plate through a mortise lock mechanism, a spraying box is installed at the position, located above the frosted plate, in the peeling box, and water tanks are fixedly connected to the outer surfaces of the two sides of the peeling box correspondingly; according to the device, spraying water can be continuously added into the spraying box through the pumping and pressing mechanism while the motor drives the centrifugal disc and the frosted plate to rotate and peel, so that the surfaces of sweet potatoes can be isolated from air by utilizing the spraying water, and the surfaces of the peeled sweet potatoes can be prevented from generating browning and breeding bacteria; therefore, the peeling quality of the device is greatly improved.

The invention discloses a cyclamen tissue culture propagation method taking ovules as explants, and belongs to the technical field of plant tissue culture. The method comprises the steps of ovule inoculation, callus induction and screening, bud induction and proliferation, strong seedling culture, rooting induction and acclimatization and transplantation. According to the method, casein hydrolysate nutrition supporting, dynamic hormone regulation and control, bud cluster-shaped proliferation, strong seedling rooting, combination of in-bottle seedling hardening and out-bottle seedling hardening, transplanting matrix improvement and other methods are adopted, and an effective new way is provided for improving the cyclamen tissue culture inoculation survival rate and the seedling emergence survival rate.

The invention relates to the technical field of microorganisms and the field of food, in particular to lactobacillus plantarum for inhibiting brown stain of soybean paste and application of lactobacillus plantarum. The lactobacillus plantarum is screened from traditional naturally-fermented peasant soybean paste in northeast China, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 30, 2025, and the preservation number is CGMCC No.35048. The lactobacillus plantarum has an obvious browning inhibition effect and can be widely applied to fermented food production.

The invention discloses an Aksu rock candy core apple storage, fresh-keeping and pretreatment integrated device, which comprises a film coating integrated device capable of sequentially carrying out cleaning, film coating and draining (or cold wind treatment) treatment on fruits passing through the film coating integrated device; the conveyor is connected with the input end part of the film coating integrated equipment; and the cold air blow-drying device is internally provided with a cold air blow-drying conveying mechanism, the cold air blow-drying conveying mechanism is connected with the output end of the film coating integrated device, and the cold air blow-drying device can conduct cold air blow-drying treatment on fruits passing through the cold air blow-drying device. In the device disclosed by the invention, after a 1% chitosan + 1% gamma-aminobutyric acid aqueous solution is used for coating treatment, the apple cores are stored at 2-4 DEG C in combination with a 1-MCP preservative, so that browning of the apple cores can be effectively inhibited, the retention time of the sugar cores of the rock candy core apples can be prolonged, and the characteristics of the Aksu rock candy core apples can be better maintained.

The present invention relates to a method for freezing and refrigerating chestnut kernels for preservation, and the specific method includes: sterilizing the chestnut kernels with a composite specific bactericide in coordination with slightly acidic electrolyzed water after pre-treatment of the chestnut kernels, then color-protecting the chestnut kernels with L-cysteine ​​in combination with cold shock treatment, and finally slightly freezing and air conditioning after treating the chestnut kernels with chitosan coating. The present invention uses a specific bactericide needle combined with the synergistic effect of slightly acidic electrolyzed water to completely kill the pathogenic bacteria of chestnuts during storage; L-cys is used to change the molecular spatial structure of PPO that causes browning during chestnut kernel storage, thereby achieving the purpose of inhibiting PPO activity; a microenvironment with high CO2 and low O2 is formed on the surface of the fruit by using a chitosan coating treatment method, and combined with cold shock treatment, the respiration intensity can be reduced to inhibit the activity of PPO polyphenol oxidase and POD peroxidase, while also inhibiting the activity of PAL phenylalanine ammonia lyase, thereby reducing the synthesis of phenolic substances.

The invention discloses application of aconitic acid in inhibition of browning of fruits and / or vegetables and inhibition of microorganisms. The use method of the aconitic acid is simple, and the aconitic acid only needs to be added into fruit and vegetable pulp or prepared into an aconitic acid aqueous solution to be used for soaking fresh-cut fruits and vegetables which are cleaned, disinfected, peeled and cut. Experiments prove that aconitic acid can effectively inhibit browning of apple, potato and snow pear slurry, fresh-cut apple slices and shredded potatoes, maintain the original color and flavor of the apple, potato and snow pear slurry, fresh-cut apple slices and shredded potatoes, maintain the original flavor of fresh-cut cucumbers and inhibit the total number of bacterial colonies of fresh-cut fruits and vegetables; the medicine has the advantages of safe use and no toxic or side effect. The treatment method for inhibiting browning and microorganisms of the fresh-cut fruits and vegetables by using aconitic acid provided by the invention is simple, easy to operate and popularize, low in cost and suitable for large-scale production and use.

The invention provides a culture method for obtaining high-quality hemsley rockvine root calluses, belongs to the technical field of tissue culture, and aims to solve the problems of slow growth, high adventitious root occurrence rate, premature aging of the calluses, serious browning, high death rate after subculture and the like in the hemsley rockvine root callus growth process. The culture method comprises the following steps: step 1, pretreatment of explants; step 2, callus induction culture: putting the treated explant into a callus induction culture medium, and putting the callus induction culture medium in an environment with a day-night temperature difference for callus induction culture; and step 3, subculture and enlarged culture of the callus: stripping the callus generated on the stem segment in the step 2, transferring the callus to a subculture multiplication medium, adjusting the light quality ratio of red light to blue light, and carrying out subculture in an environment with gradually changed day temperature to obtain the high-quality hemsley rockvine root callus. In the subculture process, the content of zeatin is increased, the light quality ratio is adjusted, and the high-quality hemsley rockvine root callus can be obtained in a circulation management mode that the daily temperature is gradually changed.

The invention discloses cold shock induced autolytic saccharomyces cerevisiae as well as a construction method and application thereof. The cold shock induced autolytic saccharomyces cerevisiae expresses a gene for coding and decomposing a cell wall component, and the gene for coding and decomposing the cell wall component comprises a coding gene bgl2 of incision beta-1, 3-glucanase, a coding gene cts1 of chitinase and a coding gene manA of beta-mannase; the promoter for coding the gene for decomposing the cell wall component is a cold shock inducible promoter. According to the prepared cold shock induced autolysis saccharomyces cerevisiae, engineering bacteria can be induced to generate obvious autolysis through one-time cold shock, meanwhile, reaction products do not need to be sterilized at high temperature, and the browning phenomenon caused by the Maillard reaction of polysaccharide and protein in filtrate under the high-temperature condition is avoided.

The invention discloses a red apple juice beverage and a preparation method thereof. The red apple juice beverage comprises 100 parts of freshly squeezed apple juice and 0.1-0.5 parts of water chestnut extract; the freshly squeezed apple juice comprises Fuji apple juice and red apple juice; the water chestnut extract comprises a supernatant extract and a precipitate extract. The water chestnut is pulped, treated with steam, and enzymatically hydrolyzed to obtain a supernatant and a precipitate; the supernatant and the precipitate are ultrasonically treated respectively, and the supernatant is extracted with ethanol to obtain a supernatant extract; the precipitate is extracted with dilute acid to obtain a precipitate extract, and the supernatant extract and the precipitate extract are mixed to obtain a water chestnut extract. The present invention enzymatically hydrolyzes water chestnuts (including peels), and then separately extracts the obtained supernatant and precipitate, and the obtained water chestnut extract is added to the freshly squeezed apple juice, which can not only reduce the content of polyphenol oxidase in apple juice and prevent browning, but also delay juice stratification, and will not affect the taste of apple juice.

The invention relates to the technical field of improved variety breeding of crops, in particular to an efficient breeding method for healthy sugarcane seedlings, which comprises the following steps: step 1, taking materials and inducing; step 2, callus differentiation; step 3, multiplication culture; step 4, root promoting culture; 5, heeling in and transplanting: transplanting rooted tissue culture seedlings outdoors, carrying out single-plant field planting to obtain healthy sugarcane original seedlings, and transplanting the healthy sugarcane original seedlings into a field; step 6, tillering pruning and cuttage: after the sugarcane original seedlings are tillered, cutting off main stems, and performing cuttage breeding through a cuttage device after soaking treatment. According to the method, a large number of healthy sugarcane seedlings are obtained through the tissue culture technology, the seedlings have the advantages of being high in growth speed, good in quality and the like, meanwhile, an oblique cutting mode in the cutting technology is combined, tillering stems are reused, the breeding efficiency is improved, and then efficient breeding of the healthy sugarcane seedlings is achieved.

The invention relates to the field of food, in particular to a stabilizer for improving the stability of Chinese chestnut sauce, a preparation method of the stabilizer and application of the stabilizer in improving the properties of the Chinese chestnut sauce. In the stabilizer, trehalose has good water binding capacity and can reduce the water activity in a system and hinder recrystallization of starch, and hydroxyl groups of trehalose molecules can interact with the starch to form a stable structure and hinder movement of water molecules, so that the stability of the Chinese chestnut sauce is improved; palmitic acid in the palm oil is a saturated fatty acid, can maintain relatively high fatty acid unsaturated index and fatty acid unsaturation degree of the Chinese chestnut sauce, can relieve peroxidation of the Chinese chestnut sauce, effectively maintains integrity and stability of the Chinese chestnut sauce, and delays occurrence of browning of the Chinese chestnut sauce; sodium alginate and xanthan gum have good film-forming properties, and can effectively limit internal and external gas exchange and isolate microorganisms. Therefore, the stabilizer provided by the invention can improve the stability of the Chinese chestnut sauce.

The invention belongs to the technical field of Schima superba tissue culture seedlings, and is specifically related to a tissue culture seedling method of a Schima superba excellent family. A combination of primary culture, subculture, root culture, domestication and seedling hardening methods is adopted, wherein two culture media are used for alternating culture in subculture, one for promoting budding, and the other for making the seedlings more robust and the leaves more stretched, and the alternating culture makes the Schima superba seedlings maintain the high quality of the subculture seedlings while maintaining a high value-added coefficient, which is conducive to subsequent root culture, and camphor essential oil / hydrosol or Melaleuca alternifolia essential oil / hydrosol is added to the culture medium, which is conducive to antibacterial disinfection, prevents the culture medium from getting moldy and the explant from browning, and in the domestication and hardening of the Schima superba tissue culture seedlings, firstly, the roots are stained with yellow heart soil slurry, and the root system will be more capable of retaining water after being wrapped with a layer of mud, and the yellow heart soil and perlite in the cultivation medium ensure the water retention and air permeability of the matrix, so that the transplant survival rate is greatly improved, and finally high-quality tissue culture seedlings are obtained.

The application discloses a method for reducing the browning rate of Zanthoxylum bungeanum in tissue culture, and belongs to the technical field of Zanthoxylum bungeanum tissue culture. The method is characterized by adopting polyvinylpyrrolidone and modified chitosan as raw materials to prepare a prevention and control agent, coating the tissue culture material with the prevention and control agent, inoculating the coated tissue culture material into a culture medium, and then carrying out tissue culture, so that the phenolic substances released by the tissue culture material and the quinone products formed by oxidation are adsorbed and fixed and covalently combined, the damage to the tissue culture material is reduced, the browning rate of Zanthoxylum bungeanum in tissue culture is effectively reduced, the propagation efficiency of Zanthoxylum bungeanum in tissue culture is ensured, and the method has a good application prospect in the field of Zanthoxylum bungeanum tissue culture.

The invention relates to the technical field of microorganisms, and particularly discloses bacillus subtilis KG25 and application thereof. The bacillus subtilis KG25 is preserved in the China Center for Type Culture Collection on September 1, 2025, the classification name of the bacillus subtilis KG25 is bacillus subtilis KG25, and the preservation number of the bacillus subtilis KG25 is CCTCC (China Center for Type Culture Collection) NO: M 20251927. The bacillus subtilis KG25 provided by the invention can be used for synthesizing surfactin with relatively high content, has a function of inhibiting enzymatic browning, and has a very remarkable fresh-keeping function on fresh-cut fruits and vegetables. The composite preservative containing the bacillus subtilis KG25 fermentation liquor provided by the invention is used for soaking fresh-cut fruits and vegetables, so that the normal-temperature storage period can be prolonged to 7 days, and the composite preservative has a wide application prospect in the field of fruit and vegetable preservation.

The present invention provides a type of ammonium cyclopentane salt and its preparation method and application. The ammonium cyclopentane salt is represented by the following structural formula (I), wherein the group R is selected from a hydrocarbon group with a carbon number of 1 to 22. The ammonium cyclopentane salt of the present invention can be used in the medical field and the daily chemical field to play the effects of freckle removal, whitening, anti-inflammatory, soothing, repairing, antibacterial and moisturizing; or used as a surfactant to play the functions of cleaning, emulsification, solubilization and dispersion. #imgabs0#

The invention discloses a color-protecting bacteriostatic agent for long-term fresh-keeping storage of fresh-cut potatoes and a preparation method of the color-protecting bacteriostatic agent, and the color-protecting bacteriostatic agent comprises 0.5-1 wt% of a tea polyphenol solution and has good color-protecting and bacteriostatic effects. The preparation method of the color-protecting bacteriostatic agent can reduce the decomposition of antioxidant components of the color-protecting bacteriostatic agent. The invention also discloses a long-term fresh-keeping storage method of the fresh-cut potatoes, which comprises the following steps: step 1, cleaning the potatoes, and immersing the cleaned potatoes in flowing water for treatment to obtain the fresh-cut potatoes; step 2, soaking in a color-protecting bacteriostatic agent for 3-5 minutes; and step 3, draining water, sealing and packaging, and storing at 2-4 DEG C. According to the long-term fresh-keeping storage method, the fresh-keeping period of the fresh-cut potatoes can be prolonged, and browning and microorganisms of the fresh-cut potatoes are effectively inhibited.

The invention discloses a preparation method of calcium threonate, which comprises the following steps: (1) adding a certain amount of vitamin C into a closed high-pressure reaction kettle, degassing, injecting 99% pure oxygen, heating and stirring for dehydration reaction; (2) transferring calcium oxide powder into a reaction kettle by using a powder transfer machine, continuously degassing for a period of time, heating and stirring to generate calcium hydroxide; (3) injecting hydrogen into the reaction kettle after the hydroxylation reaction of calcium oxide, heating and stirring to synthesize calcium ascorbate; (4) after calcium ascorbate reaction synthesis, injecting 99% pure oxygen, heating and stirring to carry out calcium threonate synthesis reaction; and (5) after the synthesis reaction of the calcium threonate is completed, discharging nitrogen into a water recovery tank connected with the lower part of the reactor. The method is short in process route and free of solid waste, the prepared calcium threonate product is stable in performance, the purpose of balancing and supplementing microelements can be achieved, the process is simple, and the method is suitable for large-scale industrial production.

This invention provides a method for producing allulose using an allulose-producing enzyme having high pH stability in a low-pH region. The method comprises a step of allowing a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 6 or a polypeptide consisting of a sequence having 90% or higher identity to the amino acid sequence shown in SEQ ID NO: 6 and capable of producing allulose to react with fructose under acidic conditions in which pH is less than 6.

The invention belongs to the technical field of plant tissue culture, and discloses a Paeonia fulva tissue culture and rapid propagation method for inducing cluster buds by using immature embryos, which comprises the following steps: collecting fresh seeds at a specific development stage, sterilizing, and stripping embryos aseptically; inoculating the embryos into a germination culture medium containing 6-BA, IAA and GA3 to start culture; after radicle tail ends of the germinating embryos are cut off, the embryos are directionally inoculated to a subculture medium according to the original polarity, and sterile seedlings are obtained; transferring the seedlings into an induction culture medium containing 6-BA, IAA and charcoal to induce cluster buds, and further proliferating; cutting robust tender shoots, removing cotyledons, and inoculating into a rooting culture medium containing IBA (indolebutyric acid), NAA (naphthylacetic acid) and charcoal to induce rooting; and finally, carrying out strong seedling culture and domestication transplanting to obtain a survived plant. According to the method, the deep dormancy obstacle of the seeds is broken through, efficient and stable regeneration from embryos to seedlings is achieved, the breeding cycle is short, the rooting and transplanting survival rate is high, and the method is suitable for large-scale protection and production of rare resources.

The invention discloses a production method of high-dietary-fiber cat litter bean dregs. The production method comprises the following steps: soaking soybeans in water and cleaning; the pretreated soybeans and water are mixed and ground into thick liquid, the obtained ground paste is subjected to high-speed centrifugation and then separated, and soybean milk and wet bean dregs are obtained; adding water into the obtained wet bean dregs and stirring; and sequentially carrying out filter pressing, crushing, steam explosion, airflow flash drying and dry crushing on the wet bean dregs which are stirred by adding water to obtain the high-dietary-fiber cat litter bean dregs. The bean dregs generated in the bean product processing process are subjected to high-value development and utilization, and the dried bean dregs for the cat litter, which are low in moisture content and protein content, high in dietary fiber content, easy to store and transport, safe, sanitary, good in water absorption, easy to loosen without caking, clean and sanitary, are prepared.