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Composition for preventing browning of plant tissue culture and using method of composition

A technology of plant tissue culture and composition, applied in the field of plant tissue culture, to achieve the effects of reducing production and expansion, convenient operation, and preventing browning

Inactive Publication Date: 2013-04-03
SUZHOU HEMEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have reduced the browning rate in tissue culture to a certain extent, there is still room for further improvement.

Method used

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  • Composition for preventing browning of plant tissue culture and using method of composition
  • Composition for preventing browning of plant tissue culture and using method of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] In the tissue culture of strawberry, MS medium is used as the basic primary medium, and different antioxidants, oxidase system enhancers and adsorbents are added to it, and the statistics of the browning of the primary culture are as follows:

[0021] Application of Strawberry Primary Culture

[0022]

[0023] Among them, 0.001-0.5 mg / L BR can be replaced by 50-150 mg / L SA, and 5-150 mg / L PVP can be replaced by 1-5 g / L activated carbon.

Embodiment 2

[0025] In the subculture of yew callus, the optimized MS medium (with the addition of 0.5 mg / L NAA, 0.5 mg / L 6-BA and 0.08 mg / L 2.4-D) was used as the basic primary generation Medium, in which different antioxidants, oxidase system enhancers and adsorbents are added, the statistics of the browning of the primary culture are as follows:

[0026]

[0027] Among them, 0.001-0.5 mg / L BR can be replaced by 50-150 mg / L SA, and 5-150 mg / L PVP can be replaced by 1-5 g / L activated carbon.

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Abstract

The invention discloses a composition for preventing browning of a plant tissue culture. The composition contains a direct antioxidant against phenolic substances and a plant antioxidase system reinforcing agent, and can play the systematic synergic action of two anti-browning mechanisms during the plant tissue culture process, on the one hand, the anti-oxidation action of the anti-oxidant can be realized against the phenolic substances infiltrating into a culture medium so as to reflect the direct reduction action, on the other hand, the plant antioxidase system reinforcing agent can reinforce the activity of antioxidase in strawberry tissues in plant tissues, and the production and the expansion of basic factors of the browning, namely quinone type substances can be reduced; and the combination of the two can effectively prevent the browning phenomenon in primary culture of strawberries and reduce the browning rate from the conventional level of above 30% to below 20%. The composition disclosed by the invention is widely suitable for various plants which are prone to browning in different tissue culture stages. The invention further discloses a using method of the composition.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a composition for preventing browning of plant tissue culture and an application method thereof. technical background [0002] Currently, in many plant tissue culture processes, browning is one of the most important factors limiting the success rate. Browning often occurs in primary culture of explants, subculture of plant callus, suspension cell culture, and isolation and culture of protoplasts. Browning products not only brown explants, cells, medium, etc., but also inhibit many enzymes, thereby affecting the growth and differentiation of culture materials, and even leading to death in severe cases. [0003] At present, it is believed that browning in plant tissue culture is mainly caused by enzymatic browning, that is, the browning of culture materials is mainly caused by phenolic compounds secreted by wounds. Its occurrence must have three conditions, namely en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00A01H4/00
Inventor 毕德
Owner SUZHOU HEMEI BIOTECH
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