72 results about "Aminoisobutyric acid" patented technology

The invention discloses an application of alpha-aminoisobutyric acid or beta-aminoisobutyric acid in preparation of a fruit preservative as well as the fruit preservative and a using method. Experiments demonstrate that after the fruit is processed by alpha-aminoisobutyric acid or beta-aminoisobutyric acid, the rotting rate of fruit can be effectively reduced, the fruit quality can be maintained,and the preserving time can be prolonged. Thus, the alpha-aminoisobutyric acid or beta-aminoisobutyric acid can be used for preparing the fruit preservative which contains 1-100 mM of alpha-aminoisobutyric acid or beta-aminoisobutyric acid. By processing the fruit such as litchi, longan, yangtao, papaya and mango by use of the fruit preservative and according to the processing method disclosed bythe invention, the rotting rate of fruit can be effectively reduced, the fruit quality can be maintained, the commodity rate of fruit can be improved, and the preserving time can be prolonged. The fruit preservative disclosed by the invention is safe and environmentally-friendly, and the preserving method is simple and convenient.

The invention discloses a metabonomics-based diagnosis marker for chronic obstructive pulmonary disease and application thereof. The diagnosis marker comprises the following 28 blood plasma metabolismmarkers: phosphatidylcholine PC 16:1-36:3, phosphatidylcholine PC 26:0-22:4, phosphatidylcholine PC 26:0-22:3, phosphatidylcholine PC 47:5e, phosphatidylcholine PC 44:11e, phosphatidylcholine PC 16:0-24:5, phosphatidylcholine PC 20:2-20:3, phosphatidylcholine PC 40:10, phosphatidylcholine PC 38:9e, phosphatidylcholine PC 18:1-20:4, phosphatidylcholine PC 16:0-20:3, phenylacetaldehyde, dihydrouracil, nicotinamide, 4-methoxycinnamic acid, ketovaline, threonine, DL-3-aminoisobutyric acid, pyruvic acid, stachyose, caffeic acid, N, N-dimethylaniline, maltotriose, D(-)-gulonate-gamma-lactone, and L-asparaginase. The marker provided by the invention has good classification on metabolome data of patients suffering chronic obstructive pulmonary disease and healthy people, and can accurately distinguish the patients suffering chronic obstructive pulmonary disease from the healthy people.

The invention provides novel amino acid compounds of use in detecting and evaluating brain and body tumors. These compounds combine the advantageous properties of α-aminoisobutyric acid (AIB) analogs namely, their rapid uptake and prolonged retention in tumors with the properties of halogen sustituents, including certain useful halogen isotopes such as fluorine-18, iodine-123, iodine-124, iodine-125, iodine-131, bromine-75, bromine-76, bromine-77, bromine-82, astatine-210, astatine-211, and other astatine isotopes. In addition the compounds can be labeled with technetium and rhenium isotopes using known chelation complexes. The amino acid compounds disclosed herein have a high specificity for target sites when administered to a subject in vivo. Preferred amino acid compounds include [¹⁸F] FAMP, ([¹⁸F]5a) and [¹⁸F]N-MeFAMP, ([¹⁸F]5b). The invention further features pharmaceutical compositions comprised of an α-amino acid moiety attached to eiher a four, five or a six member carbon-chain ring. The labeled amino acid compounds are useful as imaging agents in detecting and/or monitoring tumors in a subject by Positron Emission Tomography (PET) and Single Photon Emission Computer Tomography (SPECT).

The present invention exploits the discovery that amounts of uracil and thymine metabolites, especially β-aminoisobutyric acid, in various bodily fluids, especially urine, are correlated with the occurrence of epilepsy when compared to matched control subjects. Analytical and diagnostic protocols, including a novel high performance liquid chromatography system, for use in the invention are disclosed.

The invention aims at the field of chemical industry and relates to a method for preparing alpha-aminoisobutyric acid. The method comprises the step of enabling acetone cyanohydrin and ammonium carbonate to be subjected to pressurized and heated reaction in an aqueous medium, thereby synthesizing alpha-aminoisobutyric acid, wherein ammonium carbonate can be replaced with ammonium bicarbonate or ammonia and carbon dioxide. The invention further relates to preparation using an alpha-aminoisobutyric acid production device, wherein a gas stripping device is especially used for recovering carbon dioxide and ammonia gas which are generated during synthesis reaction and are recycled for the synthesis of alpha-aminoisobutyric acid. According to the method disclosed by the invention, the yield is high, the purity of a product is high, and the equipment investment is small; in a whole process, carbon dioxide and ammonia are hardly consumed, no byproduct inorganic salt is produced, and inorganic base is not consumed, so that a very positive effect on the full and comprehensive utilization of resources and environmental protection is exerted; recovered carbon dioxide and ammonia and crystallized mother liquor are sufficiently utilized, so that the problem of pollution to environment caused by waste gases, waste water and waste residues can be excellently solved, and the environmental-friendly and clean production is really achieved.

The invention provides a preparation process of a prostatic cancer medicine Enzalutamide. The preparation process includes: with N-methyl-4-bromo-2-fluoro-benzamide being a raw material, performing acatalytic nucleophilic substitution reaction with 2-amino isobutyric acid under alkaline condition to generate 2-(3-fluoro-4-(methylcarbamoyl)phenylamino)-2-methylpropionic acid; performing esterification to generate 2-methoxyethyl-2-((3-fluoro-4-(methylcarbamoyl)phenyl)amino)-2-methylpropionate; performing a ring closing reaction on the product with 2-trifluoromethyl-4-isothiocyanobenzonitrile togenerate the finish product 4-(3-(4-cyano-3-trifluoromethyl)phenyl)-5,5-dimethyl-4-oxo-2-sulfoimidazole-1-yl)-2-fluoro-N-methylbenzamide, namely, Enzalutamide. The method overcomes the major defectsin the prior art and avoids use of high-toxic reagent such as iodomethane, is gentle in reaction conditions and is convenient and simple in after treatment, is improved in total yield and reduced in reaction time, is reduced in preparation cost, is green and environment-friendly, and is suitable for industrial large-scale production.

The invention provides a rapid non-derivative liquid chromatography-tandem mass spectrometry detection method for free amino acids, and belongs to the technical field of quantitative detection of amino acids. A sample extract is composed of FA with the final concentration of 0.1%, methanol with the final concentration of 50% and acetonitrile with the final concentration of 50%, and the detection method adopts liquid mass spectrometry-tandem mass spectrometry for detection. A mobile phase A of liquid phase mass spectrometry is an aqueous solution containing 0.11% of formic acid and 10mM of ammonium formate, and a mobile phase B is a 90% acetonitrile aqueous solution containing 0.11% of formic acid and 10mM of ammonium formate. 33 amino acids can be combined and detected, and four groups of isomers can be distinguished at the same time,namely alanine, sarcosine and beta-alanine; 2-aminobutyric acid, gamma-aminobutyric acid and 3-aminoisobutyric acid; leucine, and isoleucine; 1-methyl histamine and 3-methyl histamine; sPE or derivation and other methods are not needed, and the pretreatment process is simple, convenient and easy to operate.

The invention discloses a siraitia grosvenori preservation filming liquid which consists of shellac, glycerol monostearate, tea saponin, penthiopyrad and alpha-amino isobutyric acid, wherein the mass concentration of shellac is 7-10%, the mass concentration of tea saponin is 0.3-0.5%, the mass concentration of penthiopyrad is 0.001-0.004%, and the mass concentration of alpha-amino isobutyric acid is 0.01-0.001%. Through the adoption of the preservative, the freshness cost is lowered, no cold tolerance pathogenic bacterium is generated, growth of bacteria on the surface of siraitia grosvenori is inhibited, and rot is prevented. The surface of the siraitia grosvenori can be smooth and fresh by using the siraitia grosvenori preservation filming liquid, the fruit rot rate is less than 2% after 100 days, and the driage is less than 20%.

The invention belongs to the field of pharmaceutical chemicals, and particularly relates to a liquid-phase preparation method of a sermaglutide side chain. The preparation method comprises the steps: reacting N-protected-L-histidine with N-hydroxysuccinimide to obtain active ester, reacting the active ester with a TMS derivative obtained by reacting 2-aminoisobutyric acid with N,O-bis (trimethylsilyl)acetamide to obtain a product, and deprotecting the product to obtain the sermaglutide dipeptide side chain compound. The liquid-phase synthesis method provided by the invention simplifies the operation steps; and compared with a conventional solid-phase synthesis method, the liquid-phase synthesis method does not cause resin polycondensation, enhances the degree of amino acid coupling, improves the reaction activity and efficiency of coupling, is beneficial to large-scale production of sermaglutide, and meets the requirements of industrial production.

The invention discloses a production method for 3-aminoisobutyric acid. A 3-aminoisobutyric acid producing strain is constructed through the following steps: A, knocking out the ptsG, fumAC and fumB genes in the MG1655 genome of Escherichia coli so as to obtain the gene knockout strain MG1655 (delta<ptsG>delta<fumAC>delta<fumB>); B, constructing of expression plasmids expressing the genes panD, aspA and C24MTgm; and C, transforming the plasmids obtained in the step B into the gene knockout strain MG1655 (delta<ptsG>delta<fumAC>delta<fumB>) obtained in the step A so as to obtain a genetically-engineered strain. The Escherichia coli engineered strain constructed in the invention can produce 3-aminoisobutyric acid, and the yield of aminoisobutyric acid produced through shake flask fermentation is as high as 100 mg/L.

The invention discloses a mango preservation filming agent which consists of shellac, copper hydroxide, alpha-amino isobutyric acid and a solvent, wherein the mass concentration of the shellac is 10-20%, the mass concentration of the copper hydroxide is 0.3-0.5% and the mass concentration of the alpha-amino isobutyric acid is 0.001-0.0005%. The preparation method comprises the following steps: dissolving the shellac by using ethanol, adding the alpha-amino isobutyric acid and the copper hydroxide, and adding water to dilute. Through the adoption of the mango preservation filming agent, the freshness cost is lowered, cold tolerant pathogenic bacteria are not generated, the surface of the mango can be smooth and fresh by using the preservation filming agent disclosed by the invention, bacterium propagation on the surface of mango is prevented, and after 100 days, the fruit rot rate is less than 3%, and the driage is less than 25%.

The invention provides a composite marker for determining whether a subject has or is about to have esophagus cancer. The composite marker is composed of sarcosine, 1,3-diaminopropane, 3-aminoisobutyric acid, thymidine putrescine and cytidylic acid, and has diagnosis sensitivity of 93.3% and specificity of 0%.

The invention provides a marker used for detecting whether a subject gets laryngocarcinoma or not, or has a trend to laryngocarcinoma, and the marker comprises one or more of creatine, 1,3-diaminopropanes, thymidine, 3-aminoisobutyric acid, cytidylic acid and putrescine, diagnosis sensitivity can reach 93.33%, and specificity is 0%.

The invention provides a fruit fresh-keeping film coating agent and a preparation method thereof, wherein the fruit fresh-keeping film coating agent comprises, by weight, 1-5% of brasenia schreberi polysaccharide, 0.1-1% of myristic acid, 0.2-1.2% of sodium erythorbate, 0.1-0.5% of amino isobutyric acid, 0.1-0.4% of polylysine, 0.06-0.36% of plant polysaccharide gum, and the balance of water. Thepreparation method comprises: 1) mixing plant polysaccharide gum and water, placing the mixture into a water bath pot, heating to a temperature of 60-70 DEG C, stirring until the plant polysaccharidegum is completely dissolved, adding brasenia schreberi polysaccharide and myristic acid, uniformly stirring until completely dissolving, cooling to a room temperature, adding sodium erythorbate, aminoisobutyric acid and polylysine, and mixing and stirring until completely dissolving to obtain a mixed solution; and 2) filtering the mixed solution to remove undissolved substances, degassing under vacuum, standing overnight at a temperature of 4-10 DEG C, recovering to a room temperature, and carrying out vacuum degassing. According to the present invention, the fruit fresh-keeping film coatingagent can effectively reduce the decay rate of fruits, maintain the quality of fruits, improve the commercial rate of fruits, and prolong the fresh-keeping period.

The invention discloses a mango preservation film coating agent which is prepared from lac, alpha-aminoisobutyric acid and a solvent, wherein the mass percent of the lac is 10-20%, and the mass percent of the alpha-aminoisobutyric acid is 0.01-0.001%. The preservation film coating agent is prepared by dissolving the lac in the ethanol, adding the alpha-aminoisobutyric acid and diluting with water. The preservation film coating agent can lower the preservation cost, and does not generate cold-resistant pathogens. By using the preservation film coating agent, the mango has smooth surface; and after 100 days, the rotting rate is less than 3%, and the water loss is less than 28%.

The invention belongs to the field of pharmaceutical chemicals, and particularly relates to a preparation method of a semaglutide dipeptide side chain. According to the preparation method, Fmoc-His(Trt)-OH and thionyl chloride are subjected to heating reflux, and a obtained product further reacts with 2-aminoisobutyric acid to obtain the important semaglutide side chain Fmoc-His-Aib-OH; the semaglutide dipeptide side chain synthesis method provided by the invention simplifies the operation steps,that is, in the first step, Trt can be removed, carboxylic acid can be made into acyl chloride, the target product can be obtained only through two-step reaction, the cost is low, and byproducts are few; and meanwhile, the next acylation reaction speed is high, the yield is high, large-scale production of semaglutide is facilitated, and the requirements of industrial production are met.

A method for synthesizing a peptide having the sequence His-X-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Y-Glu-Phe-Ile-Ala-Trp-Leu-Val-Z-Gly-Arg-Gly is disclosed. The method includes enzymatically coupling:

• • (a) a peptide C-terminal ester or thioester having a first peptide fragment with the sequence His-X-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-(thio)ester; and
  • (b) a peptide nucleophile having an N-terminally unprotected amine having a second peptide fragment with the sequence H-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Y-Glu-Phe-Ile-Ala-Trp-Leu-Val-Z-Gly-Arg-Gly; wherein:
  • X is Ala or an α-amino-isobutyric acid (Aib) residue;
  • Y is Lys, which Lys has a free side-chain ε-amino group or of which Lys the side-chain ε-amino group is protected with a protective group or of which Lys the side-chain ε-amino group is functionalized with an amino acid or another functional group; and
  • Z is Arg or Lys.

The invention discloses a mango preservative which is prepared from lac and alpha-aminoisobutyric acid, wherein the mass percent of the alpha-aminoisobutyric acid is 0.1-0.01%. The application method comprises the following steps: dissolving the mango preservative in ethanol, diluting with water, and carrying out dip coating on the mangos in the obtained dilute solution to obtain a film. By using the preservative, the preservation cost can be lowered, and the mango has smooth surface; and after 100 days, the rotting rate is less than 3%, and the water loss is less than 30%.

The invention relates to a method for preparing a coupling product having the sequence P_q-W_v-His-X-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Y-Glu-Phe-Ile-Ala-Trp-Leu-Val-Z-Gly-Arg-Gly. The method includes enzymatically coupling

• • (a) a peptide C-terminal ester or thioester having a first peptide fragment represented by the formula P_q-W_v-His-X-Glu-(thio)ester; and
  • (b) a peptide nucleophile having an N-terminally unprotected amine having a second peptide fragment with the sequence H-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Y-Glu-Phe-Ile-Ala-Trp-Leu-Val-Z-Gly-Arg-Gly
    • wherein
      • P represents a protective group at the N-terminal α-amino function of the peptide C-terminal ester or thioester and q is an integer having a value of 1 or 0;
      • W represents one or more amino acid residues, which may be the same or different and v is an integer having a value of 1 or more representing the number of amino acid residues W;
      • X is Ala or an α-amino-isobutyric acid unit (Aib);
      • Y is Lys, which Lys has a free side-chain ε-amino group or a side-chain ε-amino group that is protected with a protective group or a side-chain ε-amino group that is functionalized with an amino acid or another functional group; and
    • Z is Arg or Lys.