Rapid non-derivative liquid chromatography-tandem mass spectrometry detection method for free amino acids

A technology of free amino acid and liquid chromatography, which is applied in the direction of measuring devices, material separation, instruments, etc., can solve the problems of inconvenient application, achieve the effect of facilitating popularization and application, and shortening the pretreatment time

Pending Publication Date: 2022-02-08
上海中科新生命生物科技有限公司
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Problems solved by technology

However, there are some problems in these detection methods, which are not convenient for clinical application.

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  • Rapid non-derivative liquid chromatography-tandem mass spectrometry detection method for free amino acids
  • Rapid non-derivative liquid chromatography-tandem mass spectrometry detection method for free amino acids
  • Rapid non-derivative liquid chromatography-tandem mass spectrometry detection method for free amino acids

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Embodiment Construction

[0036] The technical solution of the present invention will be described below in combination with specific embodiments and accompanying drawings.

[0037] A rapid derivatization liquid chromatography tandem mass spectrometry detection method for free amino acids. Firstly, various amino acids in the sample are pretreated by liquid chromatography tandem mass spectrometry, and the pretreated samples are detected by liquid chromatography tandem mass spectrometry. Quantitative analysis of 33 kinds of free amino acids in human blood components, including lysine, tryptophan, phenylalanine, methionine, threonine, isoleucine, leucine, valine , histidine, arginine, tyrosine, ornithine, citrulline, glycine, serine, aspartic acid, asparagine, glutamic acid, alanine, sarcosine, beta-alanine acid, proline, glutamine, hydroxyproline, homoarginine, 1-methylhistamine, 3-methylhistamine, 6-aminocaproic acid, 2-aminobutyric acid, γ-aminobutyric acid acid, 3-aminoisobutyric acid, alpha-aminoadi...

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Abstract

The invention provides a rapid non-derivative liquid chromatography-tandem mass spectrometry detection method for free amino acids, and belongs to the technical field of quantitative detection of amino acids. A sample extract is composed of FA with the final concentration of 0.1%, methanol with the final concentration of 50% and acetonitrile with the final concentration of 50%, and the detection method adopts liquid mass spectrometry-tandem mass spectrometry for detection. A mobile phase A of liquid phase mass spectrometry is an aqueous solution containing 0.11% of formic acid and 10mM of ammonium formate, and a mobile phase B is a 90% acetonitrile aqueous solution containing 0.11% of formic acid and 10mM of ammonium formate. 33 amino acids can be combined and detected, and four groups of isomers can be distinguished at the same time,namely alanine, sarcosine and beta-alanine; 2-aminobutyric acid, gamma-aminobutyric acid and 3-aminoisobutyric acid; leucine, and isoleucine; 1-methyl histamine and 3-methyl histamine; sPE or derivation and other methods are not needed, and the pretreatment process is simple, convenient and easy to operate.

Description

technical field [0001] The invention belongs to the technical field of amino acid detection, and in particular relates to a liquid chromatography tandem mass spectrometry detection method capable of simultaneously detecting 33 kinds of free amino acids and rapidly derivatizing them. Background technique [0002] Amino acid is a general term for a class of organic compounds containing amino and carboxyl groups. Amino acids are the basic constituent units of biologically functional macromolecular proteins, the material basis of life metabolism, and are distributed throughout the body. Amino acids can be divided into essential amino acids (the human body cannot synthesize them, and the synthesis speed is far from meeting the needs of the collective, and must be supplied by food protein) and non-essential amino acids (the human body can synthesize them by itself or obtain them from other amino acids, not necessarily directly ingested from food) . Amino acids have nitrogen bala...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 谢永明王俊霞
Owner 上海中科新生命生物科技有限公司
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