Production method for 3-aminoisobutyric acid

Abstract

The invention discloses a production method for 3-aminoisobutyric acid. A 3-aminoisobutyric acid producing strain is constructed through the following steps: A, knocking out the ptsG, fumAC and fumB genes in the MG1655 genome of Escherichia coli so as to obtain the gene knockout strain MG1655 (delta<ptsG>delta<fumAC>delta<fumB>); B, constructing of expression plasmids expressing the genes panD, aspA and C24MTgm; and C, transforming the plasmids obtained in the step B into the gene knockout strain MG1655 (delta<ptsG>delta<fumAC>delta<fumB>) obtained in the step A so as to obtain a genetically-engineered strain. The Escherichia coli engineered strain constructed in the invention can produce 3-aminoisobutyric acid, and the yield of aminoisobutyric acid produced through shake flask fermentation is as high as 100 mg / L.

Description

technical field

[0001] The invention belongs to the field of metabolic engineering, in particular, relates to a method for constructing 3-aminoisobutyric acid production strains, in particular to a method for producing 3-aminoisobutyric acid and / or β-alanine through metabolic engineering strains sour method. Background technique

[0002] 3-Aminoisobutyric acid is also called β-aminoisobutyric acid (β-AIB) or 3-amino-2-methylpropionic acid. Its chemical structure is as follows:

[0003]

[0004] 3-Aminoisobutyric acid is a non-protein amino acid, a metabolite of thymine and valine, which is excreted in urine, in which the R-configuration is from thymine, and the S configuration is from valine. The R-configuration in the liquid accounts for more than 90%. The study found that β-AIB, as the first representative small molecule myokine of the non-adrenergic activator family in the thermogenic program of white adipose tissue, has good druggability for type II diabetes and met...

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