A kind of Saccharomyces cerevisiae engineered strain with high pyruvic acid production and fermentation method thereof

Abstract

The invention discloses a genetically engineered strain of Saccharomyces cerevisiae with high pyruvic acid production. The strain is classified and named as Saccharomyces cerevisiae XY-156A, which is preserved in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee, and the preservation number is CCTCC M 2019129. The genetically engineered strain of Saccharomyces cerevisiae XY-156A of the present invention can utilize a large amount of glucose to ferment and produce pyruvic acid, and after 76 hours of fed-batch fermentation, the pyruvate concentration in the fermentation broth can reach 105 g / L, and the pyruvic acid yield is 0.5g / g glucose has potential application value in pyruvate fermentation production.

Description

technical field [0001] The invention relates to a high-pyruvate-producing Saccharomyces cerevisiae engineering strain and a fermentation method thereof, belonging to the technical field of fermentation engineering. Background technique [0002] Pyruvic acid (pyruvic acid), a light yellow to yellow transparent liquid, widely exists in the human body, animals, plants and microorganisms, and is an important intermediate product in the process of cell metabolism; pyruvic acid and its salts serve as an important platform Compounds are widely used in chemical, pharmaceutical, agricultural chemicals, food health care and other fields. For example, pyruvic acid and its salts are precursors for the synthesis of important compounds such as L-tyrosine, L-tryptophan, vitamin B6, N-acetyl-D-neuraminic acid, (R)-acetylbenzyl alcohol; In addition, it has potential applications in human health care such as anti-fatigue, increasing endurance, lowering blood fat, and losing weight. [0003]...

AI Technical Summary

Problems solved by technology

For example, most of the starting Saccharomyces cerevisiae strains used in the literature reports are auxotrophic. In the process of using synthetic medium for fermentation, additional nutrients such as essential amino acids need to be added, which increases the difficulty of fermentation control and fermentation costs; In addition, the obtained Engineering strains still have problems such as insufficient pyruvate production and too long fermentation cycle

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