359 results about "Pyruvic acid acetal" patented technology

The subject invention provides materials and methods wherein unique and advantageous combinations of gene mutations are used to direct carbon flow from sugars to a single product. The techniques of the subject invention can be used to obtain products from native pathways as well as from recombinant pathways. In preferred embodiments, the subject invention provides new materials and methods for the efficient production of acetate and pyruvic acid.

A nucleotide sequence encoding a malic enzyme and a method for preparing succinic acid using the same, more particularly, a maeB nucleotide sequence encoding a malic enzyme B having the activity of converting pyruvic acid or pyruvate to malic acid or malate, or vice versa, a recombinant vector containing the gene, a microorganism transformed with the recombinant vector, and a method for preparing succinic acid using the transformed microorganism.

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).

The invention relates to a method for biosynthesis of tyrosol in Escherichia coli and application of tyrosol, which belongs to the field of bioengineering technology. According to the method for biosynthesis of tyrosol in Escherichia coli, tyrosine or glucose is used as a substrate, (4-hydroxyphenyl)pyruvic acid is produced under the catalysis of ammonialyase coded by Escherichia coli; (4-hydroxyphenyl)acetaldehyde is produced under the action of the microzyme 4-hydroxyphenylpyruvate decarboxylase; (4-hydroxyphenyl)acetaldehyde is catalyzed by alcohol dehydrogenase so as to produce tyrosol; and a phenylacetaldehyde dehydrogenase gene of Escherichia coli is knocked out at the same time so as to block the transformation channel for (4-hydroxyphenyl)acetaldehyde into (4-hydroxyphenyl)acetic acid and promote accumulation of (4-hydroxyphenyl)acetaldehyde and transformation of (4-hydroxyphenyl)acetaldehyde into tyrosol. The invention provides a novel production approach for tyrosol; and the method lays a foundation for large-scale industrial production of tyrosol and has important economic values and social benefits.

The present invention provides a process for selectively producing an enantiomer at position 4 of an optically active compound in the cross aldol reaction of pyruvic acid and indole-3-pyruvic acid. The process comprises the step of reacting pyruvic acid with indole-3-pyruvic acid in the presence of an optically active α-amino acid containing a secondary amine and a metal ion.

The invention relates to an asymmetric synthesis method of (+)-tanshinol, which comprises the following steps: carrying out Knoevenagel condensation on the raw material heliotropin, hydrolyzing for ring opening to obtain beta-(3,4-3,4-methylenedioxyphenyl)pyruvic acid, carrying out key asymmetric reduction reaction to obtain R-beta-(3,4-dibenzyloxyphenyl)-alpha-hydracrylic acid, and finally, deprotecting to obtain the (+)-tanshinol. The method has the advantages of accessible raw material and high optical purity of the product, is simple to operate, and can implement large-scale preparation.

The invention provides a method for synthesizing isoprene from pyruvic acid and 3-phosphoglyceraldehyde, and a reconstituted cell. The pyruvic acid and the 3-phosphoglyceraldehyde are finally obtained from simple starting materials such as glucose.

The invention belongs to the technical field of organisms, and particularly relates to a preparation method of L-tyrosine or L-tyrosine derivative (L-3-hydroxy tyrosine) employing enzyme conversion. The preparation method comprises the steps of: adopting feed liquid containing pyruvic acid as a substrate; adding bacterial cells with tyrosine phenol lyase activity, or crude enzyme, ammonia water, phenol or pyrocatechol; carrying out enzymatic reaction under the conditions that the temperature is 25-55 DEG C and the pH is 7-11; and separately preparing a converted product L-tyrosine or L-dioxyphenylalanine by using an isoelectric point crystallization method. By adopting the method, the L-tyrosine or L-dioxyphenylalanine with high additional value is synthetized by using cheap pyruvic acid liquid as the substrate by an enzymic method; the preparation method has the advantages of being wide in material source, high in enzymatic conversion efficiency, simple and convenient to operate, low in production cost and the like.

A genetically engineered bacterium producing 2-phenylethanol and an applying method thereof are disclosed. The recombinant bacterium is constructed by coexpressing aromatic amino acid aminotransferase and exogenous phenylpyruvate decarboxylase (Aro10) (or indole-3-pyruvic acid decarboxylase), phenylethanol dehydrogenase or phenylacetaldehyde reductase in escherichia coli to obtain the recombinant bacterium producing the 2-phenylethanol. Glutamate dehydrogenase is coexpressed on this basis to achieve self-balancing cofactor circulation, thus increasing the yield of the 2-phenylethanol. The conversion ratio of the 2-phenylethanol prepared through the recombinant bacterium is high. The bacterium and the method have important application value in production of the 2-phenylethanol in the future.

A blood test method including (a) centrifuging a mammalian blood sample into a plasma and blood cells; (b) removing buffy coats from the blood cells to obtain red blood cells containing solutes confined therewithin; (c) washing the red blood cells with a buffered physiological saline solution and isolating the washed blood cells; (d) mixing the washed red blood cells with a buffered physiological saline solution to obtain a suspended liquid; (e) centrifuging the suspended liquid to remove a supernatant and to obtain a red blood layer; (f) mixing the red blood layer with a hypertonic solution and maintaining the resulting suspension at a temperature of 25 to 40° C. for a period of time sufficient for the solutes confined in the red blood cells to penetrate into the hypertonic solution; (g) centrifuging the suspension to obtain a supernatant containing the solutes; and (h) measuring the supernatant for at least one factor selected from a glucose concentration, a pyruvic acid concentration, a lactic acid concentration and an oxidation-reduction potential.

The invention discloses an alpha-ketoglutarate producing yeast engineering strain and a construction method thereof, which belong to the field of genetic engineering. In the method, a molecular approach is adopted, and the yeast engineering strain Y.lipolytica ACL with improved ATP-citrate lyase activity is obtained by over-expressing an ATP-ATP-citrate lyase ACL coding gene from a mouse into a strain Yarrowia lipolytica WSH-Z06 for producing alpha-ketoglutarate by a fermentation method. Compared with a parent strain, the genetic engineering strain provided by the invention can intracellularly accumulate acetyl coenzyme A with the dry cell weight (DCW) of 0.89 mM/g by adopting glycerol as the unique carbon source so as to achieve the ACL activity of 3.56 U/mg protein improved by 7.5 times, achieve the alpha-ketoglutarat yield of 45.3 g/L which is 1.29 times that of parent protein and reduce the pyruvic acid yield to 17.2 g/L which is 68.8 percent of that of the parent strain, and has vast application prospect.

The invention discloses a metabonomics-based diagnosis marker for chronic obstructive pulmonary disease and application thereof. The diagnosis marker comprises the following 28 blood plasma metabolismmarkers: phosphatidylcholine PC 16:1-36:3, phosphatidylcholine PC 26:0-22:4, phosphatidylcholine PC 26:0-22:3, phosphatidylcholine PC 47:5e, phosphatidylcholine PC 44:11e, phosphatidylcholine PC 16:0-24:5, phosphatidylcholine PC 20:2-20:3, phosphatidylcholine PC 40:10, phosphatidylcholine PC 38:9e, phosphatidylcholine PC 18:1-20:4, phosphatidylcholine PC 16:0-20:3, phenylacetaldehyde, dihydrouracil, nicotinamide, 4-methoxycinnamic acid, ketovaline, threonine, DL-3-aminoisobutyric acid, pyruvic acid, stachyose, caffeic acid, N, N-dimethylaniline, maltotriose, D(-)-gulonate-gamma-lactone, and L-asparaginase. The marker provided by the invention has good classification on metabolome data of patients suffering chronic obstructive pulmonary disease and healthy people, and can accurately distinguish the patients suffering chronic obstructive pulmonary disease from the healthy people.

The invention discloses rhodotorula mucilaginosa and a fermentation culture substance and application thereof. The rhodotorula mucilaginosa is rhodotorula mucilaginosa TZR<2014>, and the preservation number is CCTCC NO:M2015574. Liquid state fermentation is conducted on the rhodotorula mucilaginosa by means of the fermentation culture medium, the biomass of fermentation liquor is 18 g/L, and the yield of carotenoid is 36 mg/L. The rhodotorula mucilaginosa has the effects of resisting staphylococcus aureus, gastric acid and cholate, and a solid fermentation substance of the rhodotorula mucilaginosa further contains pyruvic acid which has the functions of accelerating fat consumption, increasing the muscle yield, phagocytosing free radicals in bodies and inhibiting generation of the free radicals. Therefore, the rhodotorula mucilaginosa can be used for feed additives of livestock, poultry and aquatic animals and has a wide application prospect.

It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.

The present invention relates to a nasal spray containing sodium pyruvate and a preparation method thereof, belonging to the field of medical technology, being characterized in that: the nasal spray containing sodium pyruvate comprises the following components: sodium pyruvate, pyruvic acid, an isoosmotic adjusting agent, a preservative and water. Because the sodium pyruvate is capable of alleviating nasal obstruction and inflammation caused by rhinitis, a medical fluid provided in the invention can be made into a nasal spray medical fluid which is capable of entering nasal cavities by adopting a portable spraying device. The nasal spray medical fluid is particularly suitable for cleaning and removing harmful pollutants in nasal passages, paranasal sinuses and mucosal cilia and is capable of being used in nursing after a nasal cavity operation, relieving rhinostegnosis and reducing respiratory tract irritation and risks of infection. By adding the pyruvic acid to form a buffer solution, tingling caused by using the flushing fluid to wash the cavities can be reduced, and inflammation is alleviated. Allergic symptoms can be prevented or gradually alleviated for the people of special professional who often use the product provided in the invention to wash and care nasal cavities.

The invention discloses a flammulina velutipes culture medium. The flammulina velutipes culture medium comprises raw materials in parts by weight as follows: 50-60 parts of corncob, 9-19 parts of bran, 5-8 parts of rice bran, 5-15 parts of oilseed rape meal, 5-15 parts of soybean hull, 1-2 parts of gypsum powder, 1-2 parts of shell fossil and 1-2 parts of peppermint oil. A preparation method of the flammulina velutipes culture medium comprises preparation of raw materials, bottling, sterilization and cooling, wherein preparation of raw materials comprises preparation of a dry material and preparation of a wet material; preparation of the wet material is as follows: a part of materials are mixed to sufficiently suck water, citric acid or pyruvic acid are added to adjust the pH value after alcoholic fermentation, then gypsum powder, shell fossil and a nutrition liquid are added, the mixture is treated on a shaking table at a constant temperature of 25 DEG C for 10-20 minutes, and the mixture is rotationally distillated for 1-2 hours till the water content is 50%-60%; and the cooled culture medium is used for inoculating flammulina velutipes. The flammulina velutipes culture medium is rich in iron, the use amount of rice bran and the cost are reduced, the water content in the culture medium is appropriate, and nutrition components are easy to absorb by flammulina velutipes.

The invention provides an Hcy concentration detecting method. According to the principle, oxidized form Hcy is changed into free Hcy, the free Hcy reacts with serine under the catalysis of CBS to generate L-cystathionine, and then the L-cystathionine generates Hcy, pyruvic acid and NH3 under the catalysis of CBL. The pyruvic acid generated through circular reaction can be detected through LDH and NADH, the rate of the NADH which is changed into NAD is in direct proportion to the content of the Hcy in a sample, and the content of the Hcy can be detected by detecting the lowering rate of NADH+ at the position of 340 nm. The invention further provides a high-sensitivity Hcy concentration detecting kit.

The present invention is directed to labeled compounds, of the formulae

wherein C* is each independently selected from the group consisting of ¹³C and ¹²C with the proviso that at least one C* is ¹³C, each hydrogen of the methylene group can independently be either hydrogen or deuterium, the methyl group includes either zero or three deuterium atoms, Q is from the group of sulfide, sulfinyl, and sulfone, Z is an aryl group from the group of 1-naphthyl, substituted 1-naphthyl, 2-naphthyl, substituted 2-naphthyl, and phenyl groups with the structure

wherein R₁, R₂, R₃, R₄ and R₅ are each independently from the group of hydrogen, a C₁-C₄ lower alkyl, a halogen, and an amino group from the group of NH₂, NHR and NRR′ where R and R′ are each independently from the group of a C₁-C₄ lower alkyl, a phenyl, and an alkoxy group, and the methyl group can include either zero or three deuterium atoms.

The present invention is also directed to labeled compounds of the formula

where X is from the group of —NR₆R₇ and —OR₈ where R₆ and R₇ are each independently selected from the group of C₁-C₄ lower alkyl, alkoxy and aryl and R₈ is from the group of C₁-C₄ lower alkyl, alkoxy and aryl, T is from the group of —CH₃, —CD₃, —CH₂—S—R₉, —CH₂—S(O)—R₉, and —CH₂—(O)S(O)—R₉, J is from the group of —CH(OH)—CH₂—S—R₉, —CH(OH)—CH₂—S(O)—R₉, and —CH(OH)—CH₂—(O)S(O)—R₉ where R₉ is an aryl group from the group of 1-naphthyl, substituted 1-naphthyl, 2-naphthyl, substituted 2-naphthyl, and phenyl groups with the structure

wherein R₁, R₂, R₃, R₄ and R₅ are each independently from the group of hydrogen, a C₁-C₄ lower alkyl, a halogen, and an amino group from the group of NH₂, NHR and NRR′ where R and R′ are each independently from the group of a C₁-C₄ lower alkyl, a phenyl, and an alkoxy group, and C* are each independently selected from the group consisting of ¹³C and ¹²C with the proviso that at least one C* is ¹³C.

A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

The present invention discloses L-alanine dehydrogenase mutant zymoprotein and a preparation method thereof. According to a homologous sequence secondary structure comparison result, site-specific mutagenesis of 73th lysine near an L-alanine dehydrogenase activity center of Bacilluspseudofirmus into alanine through PCR is performed to construct a mutant expression vector, and prokaryotic expression and Ni-NTA affinity chromatography purification are performed to obtain a mutant enzyme K73A, wherein specific activity of the obtained mutant enzyme K73A is 202% of specific activity the wild type, and other enzymatic properties are not changed. In addition, a turnover number Kcat on a substrate L-alanine by the mutant zymoprotein is 376.7 min<-1> and is 2.0 times the turnover number Kcat of the wild type, a turnover number Kcat on beta-NAD<+> by the mutant zymoprotein is 290.1 min<-1> and is 2.1 times the turnover number Kcat of the wild type, and the mutant zymoprotein can be applicable for production processes of production of pyruvic acid, L/D-alanine, and the like through improvement biology methods.

The invention discloses a preparation for promoting fibroblasts to secrete extracellular matrix components, and a preparation method and application thereof. The preparation comprises the components of A) the egg white of eggs, B) egg white lysozyme, and C) pyruvic acid or pyruvate, can also comprise ovotransferrin and/or glucose, and is obtained by mixing the components uniformly. The preparationcan serve as an externally used paste for skin in application, and can obviously stimulate the secretion of collagen and hyaluronic acid synthase in the fibroblasts of the skin and improve the resiliency and the moisture content of the skin. The preparation is derived from a biologic extractive technique, has no harmful substance residues, safe and high-efficiency use, and is different from the prior method for promoting the secretion of a single fibroblast ECM component; besides, the preparation has multiple effects, wide application range and extensive application prospect, and can be applied in the fields of the preparation of skin substitutes, the preparation of anti-aging skin care products, cosmetics, medical treatment and the like.

The invention discloses a bio-organic fertilizer specially used in saline-alkali soil. The bio-organic fertilizer comprises, by weight, 100 parts of crop straw powder, 3 to 5 parts of humic acid, 20 to 22 parts of chitosan, 1 to 10 parts of diatomite, 4.6 to 4.8 parts of a ferment bacterial fertilizer, 5 to 6 parts of shell powder, 5 to 6 parts of a solid inoculum, 0.5 to 1 part of borax, 2 to 4 parts of zinc sulfate, 3 to 3.2 parts of rice husk carbon micro powder, 2.5 to 2.6 parts of gypsum powder, 2 to 3 parts of sodium dodecyl benzene sulfonate, 34 to 36 parts of pine needle rotted leaf soil, 1 to 1.2 parts of para molybdic acid, 0.9 to 1.1 parts of pyruvic acid, 1 to 1.2 parts of ulmic acid, 6 to 7 parts of nitro humic acid, 1.2 to 1.4 parts of a metal complex ion, and an appropriate amount of water.