Rhodotorula mucilaginosa and fermentation culture substance and application thereof

A technology of Rhododendron glabrata and culture medium, applied to Rhododendron glabrata and its fermented culture and application fields, can solve the problems of high air humidity and limited mining of Rhododendron species resources, so as to accelerate fat consumption, improve animal growth and improve efficiency. Product quality, low cost effect

Active Publication Date: 2016-10-12
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Southwest my country has abundant rainfall and high air humidity, and the climate is suitable for the growth of environmen

Method used

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  • Rhodotorula mucilaginosa and fermentation culture substance and application thereof
  • Rhodotorula mucilaginosa and fermentation culture substance and application thereof
  • Rhodotorula mucilaginosa and fermentation culture substance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: strain isolation and identification

[0027] Strain Isolation

[0028] Collect 100g of soil from the citrus orchard of Chongqing Citrus Research Institute, take 10g of soil samples in a 250ml triangular flask filled with 100ml of sterile water, vibrate on a vortex instrument and let it stand for a while, take 1ml of bacterial suspension and 9ml of Mix normal saline, then take 1ml of bacterial suspension and mix with 9ml of normal saline, and so on, set different concentration gradients, the gradient is 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , inoculate 200 μl of the bacterial suspension on the separation plate in turn, place it at 28°C for 2-3 days, and pick a typical single colony according to the colony shape (the colony is round or oval, viscous, and the color is pink. Color, large and thick colony, easy to provoke, micro-gloss), through the coarse sieve of the microscopic examination of Rhodotorula colloides.

[0029] PDA medium: 20g potato, 20g g...

Embodiment 2

[0035] Rhodotorulamuciladinosa TZR 2014 Large-scale fermentation of:

[0036] Glycine Rhodotorula TZR 2014 Inoculated on PDA medium, placed in a constant temperature incubator at 28°C for 36 hours, picked a seed culture medium (mass fraction, %): glucose 2%, peptone 1%, yeast powder 1%, natural pH, the rest is double-distilled water), cultivated at 28°C for 24h on a constant temperature shaker at 200rpm.

[0037]500 liters of fermentation tank liquid volume is 65% (V / V), liquid fermentation medium includes, (mass fraction, %): glucose 5%, peptone 1%, yeast powder 1.5%, pH 6.0, through 121 ℃ high temperature Steam sterilize for 20 minutes, cool down to 40°C, inoculate 10% seed solution with 24h of bacterial age, and ferment at 28°C and 200r / min for 96h, the biomass of the fermentation liquid is 18g / L, and the output of carotenoids is 36mg / L L.

[0038] After putting in the tank, the filter plate is concentrated to 1 / 8 of the original volume at a gauge pressure of 0.2-0.3 MP...

Embodiment 3

[0040] Rhodotorula gum TZR 2014 protein and amino acid content

[0041] The fermentation broth in Example 2 was centrifuged to collect the bacteria sludge, and dried at 55° C. to obtain dry bacteria powder.

[0042] Amino Acid Determination: Accurately weigh 120 mg of dry bacterial powder into a test tube, add 10 ml of 16 mol hydrochloric acid to the test tube containing the sample, shake and mix. Use an alcohol blowtorch to thin the lower 1 / 3 of the test tube to 4-6mm, vacuumize for 10 minutes and then seal the tube. The treated test tube was hydrolyzed in a sand bath in a constant temperature oven at 110°C for 22 hours, taken out and cooled to room temperature, shaken and filtered, took 1ml of the filtrate in a 50ml beaker, evaporated the filtrate to dryness with a constant temperature water bath at 60°C, added 0.02mol hydrochloric acid to dilute 4 times , Determinated by Hitachi L-8800 automatic amino acid analyzer with 0.22 μm filter membrane. The crude protein content ...

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Abstract

The invention discloses rhodotorula mucilaginosa and a fermentation culture substance and application thereof. The rhodotorula mucilaginosa is rhodotorula mucilaginosa TZR<2014>, and the preservation number is CCTCC NO:M2015574. Liquid state fermentation is conducted on the rhodotorula mucilaginosa by means of the fermentation culture medium, the biomass of fermentation liquor is 18 g/L, and the yield of carotenoid is 36 mg/L. The rhodotorula mucilaginosa has the effects of resisting staphylococcus aureus, gastric acid and cholate, and a solid fermentation substance of the rhodotorula mucilaginosa further contains pyruvic acid which has the functions of accelerating fat consumption, increasing the muscle yield, phagocytosing free radicals in bodies and inhibiting generation of the free radicals. Therefore, the rhodotorula mucilaginosa can be used for feed additives of livestock, poultry and aquatic animals and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a red yeast cellulite and its fermentation culture and application. technical background [0002] Rhodotorula gum belongs to basidiomycete, the colony is creamy, pink to red mucus, is a simple pseudohyphae, the cell shape is round, and it is a vegetative somatic cell that buds and reproduces. Rich in astaxanthin, carotenoids and beta-glucans. Carotenoids are orange-yellow, fat-soluble compounds that function as nutritional supplements and food colorants. Carotenoids cannot be synthesized in animals and must be provided by food. It is an important source of vitamin A in animals. It has the effect of nourishing the liver and improving eyesight, and can treat night blindness. Carotenoids are also antioxidants, which in turn protect against aging and various degenerative diseases caused by aging. At the same time, it has significant effects on anti-cancer, prevention o...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00A61K36/07A61P31/04A23K10/18A23K50/75A23K50/80C12R1/645
CPCA61K36/07C12N1/16C12P23/00C12N1/145C12R2001/645Y02A40/818
Inventor 唐志如毛俊霞孙志洪李貌
Owner SOUTHWEST UNIVERSITY
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