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Probiotic strains producing hyaluronic acid and uses thereof

A hyaluronic acid and strain technology, applied in the field of novel probiotic strains, can solve problems such as zoonotic disease virus infection, and achieve the effects of increasing the number, promoting lymphocyte activity, and anti-inflammatory lymphocyte activity.

Active Publication Date: 2016-08-10
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hyaluronic acid extracted from cockscombs still has concerns about zoonotic virus infection in terms of food safety, and it will be decomposed by enzymes after passing through the digestive juices in the stomach and intestines, so it cannot achieve significant effects through food intake

Method used

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  • Probiotic strains producing hyaluronic acid and uses thereof
  • Probiotic strains producing hyaluronic acid and uses thereof
  • Probiotic strains producing hyaluronic acid and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 isolates bacterial strain from breast milk

[0030] Streptococcus thermophilus TCI 633 (deposit number DSM 28121) was isolated from healthy human breast milk. Firstly, fresh breast milk samples were diluted and smeared on MRS-Cys (cysteine) agar plates to isolate Lactobacillus spp. and Streptococcus spp. Under the conditions for 48 hours, identify the colony (colony) type with a pictorial book, select at least 1 representative strain from each colony type on the agar plate, and select 5 to 10 strains with different colony types in total, and use a microscope and Gram Staining to predict possible strains.

Embodiment 2

[0031] Example 2 screening strains

[0032] 2.1 Methods of biochemical identification:

[0033] Gram staining (Gram's stain), the washed cells were fixed with 10% neutral formalin for 30 minutes, washed 4 times with phosphate buffer, and the cells in culture and the strains to be tested were subjected to Gram's staining. dyeing.

[0034] 2.2 Molecular identification methods:

[0035]Because different species of microorganisms must have their unique nucleic acid sequences, as far as bacteria are concerned, the 16S rDNA gene sequence differences of the same strain are very small, so the present invention is used to identify strains.

[0036] The present invention uses 16S DNA Broad-Range polymerase chain reaction (Simo Nikkari, Fred A. Lopez et al., Emerging Infectious Disease Journal 2002, 8, 188-194) detection to confirm the isolated strains. Use taco first TM Total DNA Extraction Kit (Ruiji Marine Biotechnology Co., Ltd., Taiwan) extracted the total DNA of the isolated s...

Embodiment 3

[0038] Example 3 Artificial Intestinal Fluid (AIS) Simulation Experiment (in vitro)

[0039] The artificial intestinal juice was modified according to the formula proposed by Hasjim et al. (Jovin Hasjim, Gautier Cesbron Lacau et al., Biomacromolecules 2010, 11, 3600-3608). Digestive enzymes including pancreatin (8mg / mL), α-amylase (13U / mL) and amyloglucosidase (1.12U / mL) were added to 0.2M acetate buffer (0.49mM MgCl 2 And 200mM calcium chloride, pH6.0), as artificial intestinal juice. The sterilized MRS medium is then added with artificial intestinal fluid to simulate the nutrients that bacteria grow in the intestinal tract and will ingest daily food nutrients, then added TCI 633 for culture, and cultivated at a human body temperature of 37°C to establish an artificial intestinal fluid environment. The bacterial fluid was collected at different time points to monitor the growth of the probiotic strains and the production of hyaluronic acid.

[0040] The plate counting metho...

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Abstract

The invention provides a novel probiotic strain which is streptococcus thermophilus TCI633 (a preservation number is DSM28121), a composition for producing hyaluronic acid and having a probiotic strain with a hyaluronic acid producing function, and the use of the probiotic strain for the production of the hyaluronic acid.

Description

technical field [0001] The present invention relates to a novel probiotic strain and its application in the production of hyaluronic acid. Background technique [0002] Hyaluronic acid (HA) belongs to linear glycosaminoglycan (glycosaminoglycan), which contains repeating disaccharides N-acetyl-glucosamine (N-acetyl-glucosamine) and D-glucuronic acid (D-glucuronic acid), distributed in the human body Connective tissue, epithelial tissue and nerve tissue; in addition, hyaluronic acid is the main component of extracellular matrix, which acts on cell proliferation and migration, and is also involved in the progression of certain malignant tumors. [0003] In the human body, hyaluronic acid is in the form of high-concentration hyaluronic acid in the skin, umbilical cord and vitreous humor. There are about 15 grams of hyaluronic acid in an average 70 kg (154 lbs) human body, but the amount of hyaluronic acid present in the human body will gradually decrease due to age. , the aver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/26A23C9/12A23L33/135A61K35/744A61P39/06A61P31/00A61P17/00A61P19/02C12R1/46
Inventor 林咏翔苏香绫余锦秀
Owner TCI CO LTD
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