Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A new type of Pediococcus lactis and its application

A technology of Pediococcus lactis and porcine-derived lactic acid tablets, applied to novel Pediococcus lactis and its application field, can solve the problems of not involving the adhesion of porcine-derived Pediococcus lactis to epithelial cells, not involving the verification effect, etc., and achieve a strong inhibitory effect , good antibacterial, strong adhesion effect

Active Publication Date: 2020-03-10
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above patents do not involve the adhesion of Pediococcus lactis to epithelial cells, nor do they involve animal experiments and other aspects of verification effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A new type of Pediococcus lactis and its application
  • A new type of Pediococcus lactis and its application
  • A new type of Pediococcus lactis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation, screening and identification of Pediococcus lactis

[0038] From the fresh feces of weaned piglets from healthy Beijing black pigs, aseptically weighed an appropriate amount and added it to sterile normal saline, oscillated evenly and made a ten-fold gradient dilution, spread it on the MRS medium plate, anaerobic tank method 37 Cultivate at ℃ for 24-48 hours. Pick milky white colonies from them for preliminary screening, all Gram-stained positive, H 2 o 2 The strains with negative enzyme test and negative contact enzyme can be preliminarily determined to be lactic acid bacteria. The strains obtained from the preliminary screening are tested for acid resistance, bile salt resistance, antibacterial and other tests, and finally a strain of lactic acid bacteria ZPA017 with excellent performance is screened out and sent to China General Microbiology Bacteria Co., Ltd. Species Collection Center (CGMCC) for identification, cell morphology characteristics...

Embodiment 2

[0045] Example 2 Study on the characteristics of Pediococcus lactis

[0046] 1. Determination of acid resistance

[0047] The activated Pediococcus acidilactici (Pediococcus acidilactici) ZPA017 obtained in Example 1 was inserted into the liquid MRS medium with a pH of 2, 3 and 4 respectively at an inoculum size of 1%, and incubated at 37°C for 2, 4 and 6 hours respectively , with 0 hour as the control, the number of viable bacteria was measured, and the survival rate was calculated. The test results are shown in Table 2.

[0048] Table 2 Acid resistance of Pediococcus lactis (%)

[0049]

[0050] As can be seen from the results, this Pediococcus lactis acts in the acidic environment of pH=2 for 2 hours, and its survival rate is 98.45%, and the survival rate at the time of 4 hours is 82.17%, and the survival rate at the time of 6 hours is 77.91%; In the environment of 3, the survival rate at 2 hours was 100.78%, the survival rate at 4 hours was 88.33%, and the survival r...

Embodiment 3

[0072] Embodiment 3, the preparation of Pediococcus lactis preparation

[0073] 1. Fermentation of Pediococcus lactis

[0074] The Pediococcus acidilactici ZPA017 (preservation number: CGMCC No.9040) obtained in Example 1 was inoculated in the MRS liquid medium with an inoculum size of 1%, and cultured at 37°C for 24 hours, so that seeds were obtained after 2 generations of activation Culture: Inoculate the seed culture with a 5% inoculum size in a 50L fermenter equipped with 30L optimized liquid medium, set the temperature at 37°C, rotate at 100rpm, and maintain a constant pH of 4.8, when fermented to 16 hours Feed feeding was started, and 200 mL of feed liquid was added to the fermenter at a time, and then fed every 2 hours for a total of 5 feeds; when the pH of the fermentation broth no longer decreased, the fermentation was terminated.

[0075] Wherein, the optimized liquid medium is as described in Example 2. The feeding liquid is glucose solution with a concentration o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a new strain of Pediococcus acidilactici (Pediococcus acidilactici) ZPA017, the preservation number of which is CGMCC No.9040, which has the characteristics of acid resistance, bile salt resistance, strong adhesion and good bacteriostasis. The invention also discloses a preparation containing Pediococcus lactis ZPA017. The preparation is used as a feed additive to feed weaned piglets, which can inhibit the reproduction of harmful bacteria, regulate the micro-ecological balance of the intestinal tract, and have the effects of enhancing body immunity and improving production performance.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a novel Pediococcus lactis and application thereof. Background technique [0002] Because the digestive system and physiological functions of weaned piglets are not yet fully developed, they are easily affected by stress factors such as weaning, feed nutrition, and feeding management, and the intestinal flora disorder occurs, causing diarrhea in piglets, which in turn leads to decreased feed intake and growth retardation. Even death, bring great loss to pig production. [0003] Pediococcus acidilactici (Pediococcus acidilactici) is a lactic acid bacterium in the Pediococcus genus, which is commonly used in the fermentation of meat, dairy products, vegetables and silage, etc. Its main advantages are: rapid growth, the ability to produce a large amount of organic acids such as lactic pH value; produce organic acids and antibacterial substances, which can inhibit the growth ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23K10/18A23K50/30C12R1/01
CPCC12N1/04C12N1/20C12N1/205C12R2001/01
Inventor 刘辉季海峰张董燕王四新王晶张伟王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com