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A kind of Lactobacillus salivarius inhibiting poultry pathogenic Salmonella and its application

A technology of Lactobacillus salivarius and Salmonella, applied in the direction of bacteria, antibacterial drugs, and methods based on microorganisms, can solve the problems of salmonellosis outbreaks, losses, etc., and achieve good adhesion, strong inhibition, and promote the effect of poultry growth

Active Publication Date: 2018-10-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the survey, the positive rate of Salmonella in farms all over my country is extremely high. Although some chicken farms control the infection at a low level by using drugs and other methods, and there is no current incidence, but if the chicken farms are not managed properly and the immunity of the chickens declines, then Prone to outbreaks of salmonellosis, leading to serious losses

Method used

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  • A kind of Lactobacillus salivarius inhibiting poultry pathogenic Salmonella and its application
  • A kind of Lactobacillus salivarius inhibiting poultry pathogenic Salmonella and its application
  • A kind of Lactobacillus salivarius inhibiting poultry pathogenic Salmonella and its application

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Embodiment 1

[0028] 1. The source of Lactobacillus salivarius SCL87

[0029] The Lactobacillus salivarius was isolated by the inventor from healthy chickens in a Guangdong farm in mid-2013, and is now preserved and provided by the Poultry Disease Laboratory of the Veterinary College of South China Agricultural University.

[0030] 2. Identification and classification of Lactobacillus salivarius SCL87

[0031] The inventor's laboratory system identification shows that the strain is Lactobacillus salivarius.

[0032] (1) Biochemical identification

[0033] Referring to the "Berger Bacteria Identification Manual", the results of the seven biochemical tests of the isolated strain SCL87, catalase test, nitrate reduction test, gelatin liquefaction test, indole test, hydrogen sulfide test, V-P test and dynamic test, were all consistent. is negative. It was preliminarily determined that the strain SCL87 belonged to the genus of Lactobacillus.

[0034] (2) PCR identification

[0035] Refer to ...

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Abstract

The invention discloses a lactobacillus salivarius for efficiently inhibiting avian pathogenic salmonella and an application of the lactobacillus salivarius. The lactobacillus salivarius is separated from healthy chicken flocks and is named lactobacillus salivarius SCL87 with the preservation number of CCTCC NO: M2015338. The strain has acid resistance, cholate resistance, trypsin resistance and good adhesion capacity. A supernatant secreted by the strain has the strong inhibition effect on the avian salmonella, and can effectively competitively inhibit growth of the avian salmonella, prevent and treat the avian salmonella and promote growth of birds. The strain can be applied to preparation of preparations for preventing infection of the avian salmonella or preparation of preparations for improving ecological balance of intestinal flora of the birds and promoting growth of the birds.

Description

technical field [0001] The invention relates to a new bacterial strain and its application in biopharmaceutical engineering. The invention relates to a Lactobacillus salivarius which inhibits avian pathogenic Salmonella and its application. Background technique [0002] Lactobacillus salivarius is one of the probiotics with important physiological functions in animals, and it is also one of the normal flora in the intestinal tract of animals. Most sialobacteria can produce organic acids, hydrogen peroxide, bacteriocins, and similar antibiotics to inhibit the growth of pathogenic bacteria. Organic acids can penetrate the bacterial membrane of pathogenic bacteria and hinder their metabolic activities such as oxidation and phosphorylation; hydrogen peroxide can cause oxidative denaturation of bacterial proteins, inactivate certain enzymes in the metabolic process, and eventually lead to bacterial death. When an animal ingests a certain amount of lactic acid bacteria, the lacti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P1/00A61P1/12C12R1/225
Inventor 罗开健王媚廖明张建民万红张宗尧黄永兴
Owner SOUTH CHINA AGRI UNIV
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