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Bacillus coagulans X26 and application thereof

A technology of Bacillus coagulans and X26, applied in the field of microorganisms, can solve the problem that additives cannot meet the actual production needs, and achieve the effect of increasing abundance

Active Publication Date: 2022-06-07
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the invention is to overcome the problem that microbial feed additives in the prior art cannot meet actual production needs

Method used

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  • Bacillus coagulans X26 and application thereof
  • Bacillus coagulans X26 and application thereof
  • Bacillus coagulans X26 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The present embodiment provides the separation and screening method of Bacillus coagulans X26, as follows:

[0023] S1. Weigh acid soil samples from vegetable fields and orchards in Shihezi, Shanxi, Shuozhou, Hubei, Huanggang, Hubei, Jiangxi, Chengdu, Sichuan, Yinchuan, Ningxia, Heshan, Guangdong, respectively. A total of 14 samples, each 3g, were mixed. Add 100ml of sterile physiological saline, extract at 37°C, 150rpm water bath shaker for 1h to obtain bacterial liquid;

[0024] S2. The bacterial solution is treated in a water bath at 80°C for 10 minutes;

[0025] S3. Inoculate the bacterial solution treated in S2 into YPD liquid medium containing 0.3% bile salts at pH 2.0 with 5% inoculum, and treat at 37°C for 2h;

[0026] S4. Inoculate the bacterial liquid treated by S3 into YPD liquid medium with 5% inoculum for enrichment, culture at 37-45°C for 18h in a water bath and shaker, and spread on MRS pH5.5 plate at 45°C Culture for 48h;

[0027] S5. Take colonies of...

Embodiment 2

[0033] This example evaluates the probiotic properties of Bacillus coagulans X26.

[0034] 1. Antibacterial test

[0035] For each pathogenic bacteria, the following method was used to prepare the experimental plate: draw 1 mL of the test pathogenic bacteria strains preserved in the glycerol tube, insert it into 100 ml of broth shake flask medium, and cultivate at 37 ° C and 150 r / min for 17 h to obtain the pathogenic bacteria liquid; 100 ml of LB agar After the medium is dissolved, cool to 48-50°C, add the pathogenic bacteria solution prepared above, shake it up quickly and pour it into a plate, 20ml per plate, put it in a horizontal position and wait for it to solidify. Oxford Cup. The pathogenic bacteria include Escherichia coli, Streptococcus broada, Staphylococcus aureus, Salmonella, Aeromonas hydrophila.

[0036] Positive control group: 1.5ug / hole ciprofloxacin;

[0037] Negative control group: YPD medium;

[0038] Test group: Bacillus coagulans X26 fermentation broth ...

Embodiment 3

[0055] In this example, the effects of Bacillus coagulans X26 on animal growth performance, blood indicators, immune organs, and intestinal structure were verified through animal breeding experiments; Effects of X26 on carbohydrate enzymes in the intestinal contents of broilers.

[0056] 1. Experimental design

[0057] 150 AA broilers were selected and randomly divided into the following two treatment groups.

[0058] Control group: 5 replicates, each replicate of 15 pigeons, fed a basal diet of corn and soybean meal;

[0059] Experimental group: 5 replicates, 15 pigeons per replicate, fed basal diet + 1×10 12 CFU / t.

[0060] The basal diet was fed with Chia Tai broiler feed, 1-21 day old with broiler chicken feed 510, and 22-42 day old with 511 in broiler chicken feed. The main raw materials are: corn, wheat, soybean meal, bran, rice bran, rapeseed meal, corn dried whole distiller's grains, stone powder, sodium chloride, amino acids, amino acid salts, vitamins and mineral...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly provides bacillus coagulans X26 which is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20211707, and a 16S rDNA sequence is as shown in SEQ ID NO.1. The bacillus coagulans X26 has the advantages that the bacillus coagulans X26 can be used for preparing bacillus coagulans X26; the bacillus coagulans X26 has the characteristics of high temperature resistance, acid resistance and cholate resistance, can be fermented to produce lactic acid, xylanase and cellulase and inhibit pathogenic bacteria, and can also be used in feed additives to improve the growth condition of livestock and poultry.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus coagulans X26 and applications thereof. Background technique [0002] The research and development of green additive products such as microbial preparations has become a development trend, which has accelerated the pace of entering the "anti-antibiotic era" in the field of feed. There are 35 kinds of microecological preparations specified in the feed additives catalog, including 7 kinds of spores, 23 kinds of lactic acid bacteria, 4 kinds of fungi, and 1 kind of photosynthetic bacteria. However, due to the particularity of the feed industry, pellet feeds need to undergo high-temperature processing, and the micro-ecological preparations added need to be able to withstand high temperatures. In addition, after the feed enters the gastrointestinal tract of animals, it also needs to be able to withstand gastric acid bile salts, etc. Therefore, although t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/24C12N9/42C12P7/56A61K35/742A61P31/04A23K10/18A23K50/75C12R1/07
CPCC12N9/248C12N9/2437C12P7/56A61K35/742A61P31/04A23K10/18A23K50/75Y02P60/87
Inventor 徐丽陈向东张成杰周樱詹志春付大波邓晓旭刘文悦
Owner 湖北华扬科技发展有限公司
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