376 results about "Ciprofloxacin" patented technology

The invention provides a preparation method of an oxidized graphene and sodium alginate composite absorbing material capable of removing ciprofloxacin in an aqueous solution. The preparation method comprises the following steps: dispersing a certain amount of oxidized graphene samples in deionized water, putting the oxidized graphene samples into an ultrasonic cleaner, and performing ultrasonic treatment for 6-10 hours; dissolving a certain amount of sodium alginate into an oxidized graphene solution subjected to ultrasonic treatment, and performing ultrasonic treatment for 20 minutes; dropewise adding the mixed solution into a 0.2mol/L calcium chloride solution by virtue of a peristaltic pump, soaking for 1-3 days, and cleaning with deionized water for 3-4 times, thereby obtaining a sodium alginate and oxidized graphene sphere hydrogel material; and freeze-drying, thereby obtaining oxidized graphene and sodium alginate sphere aerogel. The preparation method is performed under a relatively mild condition, and oxidized graphene is packaged in a sodium alginate network structure by an ion bridge method, so that the nano toxicity of graphene powder is avoided, and a new nano material capable of efficiently absorbing ciprofloxacin antibiotics in the aqueous solution is prepared. The preparation process is simple, easily controllable in condition, low in cost and suitable for large-scale batch production.

A pharmaceutical composition comprising a fluoroquinolone such as ciprofloxacin, cyclodextrin, and a hydroxy acid is described. The composition may be an aqueous composition, with such aqueous compositions preferably having a pH between 5 and 7. In some preferred embodiments, the composition further comprises a soluble polymer.

The present invention is directed to a pharmaceutical composition comprising a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. A suitable antibiotic is selected from the group consisting of doxycycline, gentamicin, tobramicin, ciprofloxacin, clindamycin, clarithromycin, azithromycin and metronidazole. Preferred antibiotics are doxycycline and ciprofloxacin. More preferably, the antibiotic is doxycycline. In its second aspect, the present invention is directed to a method for treating periodontal disease in a mammalian patient comprising administering to a mammalian patient in need of such treatment a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. Typically, the mammalian patient is a human.

The present invention relates to a method for determining 10 kinds of antibiotics in a water environment through combination of a sample pre-treatment technology and HPLC-MS, and belongs to the field of detection of safety of trace organic contaminant residue in the water environment. The method is characterized in that a water sample is separated and enriched through combination of solid phase extraction and dispersive liquid-liquid microextraction (SPE-DLLME), and then an ultra-high performance liquid chromatography-mass spectrometry instrument (UPLC-MS/MS) is adopted as a detection tool to directly determine the contents of 10 kinds of common antibiotics in the water environment (drinking water, tap water, river water, sewage treatment plant influent and effluent), wherein the 10 kinds of the common antibiotics respectively are sulfadiazine, sulfamethoxazole, oxytetracycline, tetracycline, doxycycline, ciprofloxacin, levofloxacin, chloramphenicol, cefuroxime axetil and tinidazole. According to the present invention, the water sample pre-treatment method and the instrument detection conditions are investigated and optimized, and the optimal SPE-DLLME-UPLC-MS/MS method is established and is successfully applied for the real sample determination; and compared with the traditional method, the method of the present invention has advantages of high sensitivity, high extraction recovery rate, wide application objects, environmental protection, and the like.

The invention provides a molecular imprinting catalytic membrane capable of selectively degrading ciprofloxacin and a preparation method. The preparation method comprises the following steps: step 1, preparing graphite-like nitrogen carbonate g-C3N4; step 2, preparing a noble metal loaded semiconductor composite material; and step 3, preparing the molecular imprinting catalytic membrane capable of selectively degrading the ciprofloxacin. The material prepared by the preparation method can effectively realize the aims of preferably selectively adsorbing and degrading target pollutants in a mixed sewage system; post-treatment is simple, a photocatalyst is conveniently recycled for a plurality of times and secondary pollution is effectively avoided; and the molecular imprinting catalytic membrane has the advantage of selectively treating antibiotic wastewater very well.

The invention relates to a method for preparing ciprofloxacin by piperazine reaction. The technical problems to be solved are as follows: (1) reducing amount of a solvent or using no solvent, (2) reducing consumption of the piperazine, and (3) shortening the reaction time, simplifying operation, and enhancing overall yield. The method is characterized by (a) mixing the piperazine as shown in formula (iii) with the cyclopropane carboxylic acid as shown formula (ii) at the mol ratio of 1-10:1, adding metal Lewis acid catalyst with the chemical equivalent of 1%-30% for thorough reaction in an aqueous system at the temperature of 80-150 DEG C to obtain reaction solution, wherein, the metal Lewis acid catalyst is selected from one or more of aluminum (III) Lewis acid and iron (III) Lewis acids; (b) adding inorganic base to the reaction solution at the temperature of 20 DEG C-100 DEG C to regulate pH value of the reaction system to 9-12, and filtering while the system is hot; and (c) regulating the pH value of the filtrate to 7-8 with the inorganic base for recrystallization to obtain the product.

The invention provides a C/Fe-FeVO4 composite photocatalyst as well as a preparation method and application thereof, and belongs to the technical field of preparation of inorganic nano-materials. The preparation method comprises the following steps: mixing glucose and ferrous gluconate; adding de-ionized water and stirring until a solution is clarified; dissolving substances, putting the solution into a hydrothermal reaction kettle and reacting; after cooling, taking out a product from the reaction kettle; washing with the de-ionized water and absolute ethyl alcohol; drying washed sediment in vacuum to obtain a carbon colloidal ball; adding the carbon colloidal ball into the de-ionized water and carrying out ultrasonic treatment; taking Fe(NO3)3.9H2O, NH4VO3 and an SDBS (Sodium Dodecyl Benzene Sulphonate) surfactant; adding into the carbon colloidal ball and mixing; continually carrying out the ultrasonic treatment; adjusting the pH (Potential of Hydrogen) value of the solution to obtain a grey solution; putting the grey solution into a stainless steel reaction kettle and reacting under a hydrothermal condition; cooling to room temperature to obtain a muddy solution; centrifuging the muddy solution and washing with water and ethanol respectively, and drying to obtain a C/Fe-FeVO4 precursor; roasting the C/Fe-FeVO4 precursor to obtain C/Fe-FeVO4 composed of mesoporous nano-sheet-shaped structures. The C/Fe-FeVO4 composite photocatalyst provided by the invention is used for treating newly-developing micro-pollutants of ciprofloxacin and norfloxacin under visible light.

The invention discloses a method for preparing a selectively degraded ciprofloxacin photocatalyst, and belongs to the technical fields of material preparation and environmental pollution treatment. The method comprises the following steps of: (1) hydrothermal synthesis of a ZnS semiconductor material; (2) ZnS surface modification; (3) preparation of a molecular imprinting polymer photocatalyst; and (4) elution of template modules in the imprinting polymer from the obtained solid powdery substance. The photocatalytic degradation process of the molecular imprinting polymer photocatalyst can effectively fulfill the purposes of selective identification, adsorption and catalytic degradation of target pollutants, the effective degradation efficiency of the target substance is improved, and the photocatalyst has the advantage of strong selective treatment of antibiotic wastewater.

The present invention generally relates to transdermal delivery and, in particular, to transdermal delivery using nanoemulsions and other emulsions. In one aspect, the present invention is directed to emulsions comprising a first, continuous phase and a second, discontinuous phase. The first phase may be an aqueous liquid and the second phase may comprise a lipid, such as isopropyl myristate. In some cases, a surfactant, such as Pluronic® L61, is used to stabilize the emulsion. Surprisingly, it has been found that such emulsions are effective at delivering pharmaceutically active agents, such as ciprofloxacin, when the formulation has a very low water content, for example, less than 30 wt % or less than 10 wt %. This is surprising because high water contents—not low water contents—are typically correlated with greater transdermal drug delivery, and thus, a low water content would have been considered to be unfavorable for facilitating transdermal drug delivery.

The invention discloses a chemiluminescence enzyme-linked immunosorbent test kit of ciprofloxacin, which includes a test kit box body, an enzyme label plate and reagent arranged in the test kit. The test kit is characterized in that holes of the enzyme label plate are coated with envelope antigen which is namely the conjugate of ciprofloxacin and carrier protein; the reagent includes polyclonal antibodies of the ciprofloxacin, enzyme-labeled second antibody which is namely the goat anti rabbit antibody marked by horseradish peroxidase, standard liquid of the ciprofloxacin, concentrated washing liquid and luminescence liquid. With simplicity, celerity and accuracy, the chemiluminescence enzyme-linked immunosorbent test kit has the advantages that the sensitivity is high, and the sensitivity can be enhanced by one order of magnitude compared with traditional colour comparison ELISA; thus playing an important role in ciprofloxacin residue detection of animal food (such as milk, animal tissue and urine samples).

The invention discloses a quantum dot-based method for detecting ciprofloxacin by immunofluorescence and a special kit. The immunofluorescence detection kit for detecting the ciprofloxacin comprises a ciprofloxacin peridium and a ciprofloxacin antibody marked by a quantum dot. By the method, the quantitative detection of ciprofloxacin residual medicines can be realized, the detection limit is low, the detection sensitivity is high, the specificity is high, and the method is suitable for detecting various samples. Therefore, the method has a bright application prospect in the field of the detection of the ciprofloxacin.

The invention belongs to the field of drugs and pharmaceutics and relates to a pulmonary delivery ciprofloxacin pharmaceutical composition. In particular, a liposome modified by PEG (Polyethylene Glycol) is used as a drug carrier in the composition; the liposome modified by the PEG is that PEG molecules are directly connected to the liposome; or the liposome is coated by the PEG molecules. The invention further relates to the preparation method of the composition. According to the composition disclosed by the invention, the stability is good; the action time is prolonged; the better antibacterial effect is obtained; furthermore, the pulmonary effective treatment concentration can be achieved rapidly; the bioavailability is high; the composition is convenient to carry; and the composition can be taken independently.

The invention provides a kind of product that can prevent the contamination in cell cultivating. The characteristic of the product is that every liter product contains 1-50g ethyl ciprofloxacin. The invention adds drug of certain density which can prevent and clear the contamination to the cell culture fluid to prevent the common contamination of bacteria and mycoplasma. The invention has overcome many defects of common drugs such as short drug action period, short shelf life, high price and unable to preserve in ambient temperature. The invention can not only be used in cell cultivating, it can also be used to clarify other organic medium materials, wash the commonly used apparatus or clarify the separating and purifying materials in emergency.

The invention is a kind of immune colloidal gold reagent for measuring the ciprofloaxcin and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of ciprofloaxcin resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by ciprofloaxcin-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

The invention discloses immunochromatography test paper for detecting Ciprofloxacin and a preparation method thereof. The immunochromatography test paper for detecting the Ciprofloxacin comprises a sample pad, a nitrocellulose membrane and an absorbent pad, wherein the sample pad contains a superparamagnetic compound particle marker Ciprofloxacin antibody; the nitrocellulose membrane is connected to one end of the sample pad; the absorbent pad is connected to the other end of the nitrocellulose membrane; a detection line and a quality control line which are separated with each other are coated on the nitrocellulose membrane; the detection line contains a Ciprofloxacin antigen; and the quality control line contains an antiantibody which can be specifically bound with the Ciprofloxacin antibody. Proved by the experiment of the invention, the test paper for detecting the Ciprofloxacin provided by the invention has high sensitivity, strong specificity and is rapid, simple and convenient, and objective determination can be realized.

The invention provides a novel flavonoid glycoside and isoquinoline alkaloid complex structure, preparation, antibacterial application and three complex carrier-free nano-drugs with self-assembly performance. The structural general formula of the complex is shown in the specification, the complex has excellent selective antibacterial ability, and the activity of endophyte escherichia coli and probiotics bacillus subtilis and enterococcus faecium is not influenced while pathogenic bacteria staphylococcus aureus is killed; meanwhile, the compound has a good inhibition effect on multiple drug-resistant staphylococcus aureus, and is superior to norfloxacin, oxacillin, tetracycline and ciprofloxacin. The nano-drug formed by self-assembling the three complexes is uniform in form and good in dispersity and stability, gel or fiber is formed under the condition that auxiliary materials are not needed, and support is provided for development of a carrier-free pure drug delivery preparation.

The invention discloses a detection kit for ciprofloxacin and a detection method thereof, which belong to the technical field of TRFIA and are used for detecting CIP in animal derived food, blood, urine and fodder. The prepared kit adopts the TRFIA to detect the CIP, and the measurement base is labeled immune response. A micro-pore plate is clad with CIP-carrier protein; and CIP standard or sample and CIP antibody are added into the micro-pore plate. The free CIP and the CIP-carrier protein on the micro-pore plate compete for the CIP antibody, the unconnected CIP antibody is removed by washing, then Eu3+-goat anti-rabbit antibody is added into the micro-pore plate, and the unconnected Eu3+-goat anti-rabbit antibody is removed by washing after the labeled immune response. After the enhancement liquid is added, the fluorescence intensity cps is measured by using a time-resolved fluorescence instrument, the fluorescence intensity is in inverse proportion to the concentration of the CIP in the sample, and the content of the CIP in the detected sample can be determined by referring to a stand curve. The kit provided by the invention for detecting the CIP has the advantages of simple structure, using convenience, low price and high sensitivity, and can reach over 0.01ng/mL.

The invention relates to a method for treating antibiotic and heavy metal combined pollution water by using modified graphene oxide. Specifically, nitrilotriacetic acid modified magnetic graphene oxide is taken as an adsorbent and then added to ciprofloxacin and heavy metal ion combined pollution water. An experiment result proves that each gram of the adsorbent contains almost hundreds of milligrams of exchanging ciprofloxacin by mass, the mass of exchanging copper ions reaches more than 60 milligrams, amination modified magnetic graphene oxide has a very good adsorption effect on ciprofloxacin and copper ions, and solids and liquids can be well separated after adsorption. Compared with the prior art, the treatment method is simple, the material preparation and operation cost is low, and antibiotics and heavy metals in the water can be effectively and simultaneously removed.

The invention provides a method for adsorbing and removing TC (tetracycline) and CIP (ciprofloxacin) single and compound polluted water by using a DTPA modified magnetic graphene oxide composite. Theexperiment result shows that the mass of the TC exchanged through each gram of adsorbent in the single antibiotic water is near to hundreds of milligrams, the mass of the exchanged CIP is up to more than 100 milligrams, the adsorbing effects of the DTPA modified magnetic graphene oxide on the TC and the CIP are both quite excellent, and meanwhile, the solid and the liquid can be well separated after the adsorption. The influence of the competitive adsorption on the ciprofloxacin is relatively weak in the compound polluted water, and a new thought is provided for the removal of the ciprofloxacin in the compound polluted water. Compared with the prior art, the treatment method is simple, the material preparation cost and the operation are low, a new way is provided for the treatment of the pollution of antibiotics in the wastewater, and the method can be popularized in the treatment of the antibiotic pollution as a novel adsorbent.

The invention provides Co2FeAl-LDH, a preparation method thereof and a method for degrading pollutants, and the method for the Co2FeAl-LDH comprises the following steps: dissolving nitrate of cobalt,nitrate of iron, nitrate of aluminum and urea in water to obtain a uniform solution; and reacting the uniform solution for 22 to 26 hours at the temperature of between 105 and 115 DEG C to obtain theCo2FeAl-LDH nanosheet. The Co2FeAl-LDH prepared by co-precipitating the nitrate of cobalt, nitrate of iron and nitrate of aluminum by using the urea can be used as a catalyst. By means of the synergistic effect of cobalt and iron on strongly oxidative sulfate radicals which are generated by activation of peroxymonosulfate and the carrier effect of aluminum, pollutants such as sulfamethoxazole, ciprofloxacin and tetracycline in sewage are rapidly catalyzed and degraded. The process is simple. The Co2FeAl-LDH is a heterogeneous catalyst, is easy to recycle, and has the advantages of favorable stability and favorable reusability.