12 results about "Weissella" patented technology

The invention relates to the technical field of microbial fermentation and food processing, and particularly discloses Weissella fusiformis JMF-006 as well as a mutagenesis method and application thereof. The invention provides a Weissella fusiformis JMF-006 with the exopolysaccharide yield of 20-25g / L. The Weissella fusiformis JMF-006 is obtained by breeding through a mutation breeding method of a high-yield EPS strain, and the mutation breeding method can also be used for culturing other high-yield EPS strains. The invention provides a strain composition, the strain composition comprises Weissella fusiformis JMF-006 or a high-yield EPS strain, other lactic acid bacteria strains (such as lactobacillus plantarum JMF-001 and lactobacillus casei JMF-002) can be further compounded, and the obtained strain composition has more excellent fermentation performance. When the Weissella fusiformis JMF-006, the high-yield EPS strain and the strain composition provided by the invention are applied to the fermentation process of food-derived drinks, a fermented product with stable properties and better flavor can be obtained, and the technical problems that the yield of extracellular polymeric substances is low, the fermentation performance is poor and the application range is narrow in an existing lactic acid bacteria starter are solved.

This invention belongs to the field of microbial technology, specifically relating to a method for preparing and applying a fused Weissella extracellular polysaccharide. The extracellular polysaccharide of this invention is composed of glucose monosaccharide, and the glucose linkages include terminal glucose t-Glc(p), 1→4 linked glucose, 1→4,6 linked glucose, 1→3,6 linked glucose, and 1→2,6 linked glucose, with a relative molar ratio of (8~8.5):(1~1.2):(81~82):(7~7.5):(1~1.2):(0.5~1). It possesses excellent water-holding capacity, oil-holding capacity, emulsifying properties, and emulsion stability, and can be used as a highly efficient moisturizer, emulsifier, emulsion stabilizer, cosmetic additive, pharmaceutical additive, and food additive, showing broad application prospects in the food, cosmetic, and pharmaceutical fields.

This invention discloses *Wesleyanus esculenta* strain, its fermentation product filtrate, and its biological products, belonging to the field of cosmetic technology. This strain can be used in cosmetics, exhibiting excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging capabilities, achieving good antioxidant effects. Secondly, at various concentrations, its fermentation product filtrate can significantly downregulate the proportion of SA-β-Gal positive cells and significantly inhibit the gene expression of various matrix metalloproteinases, demonstrating a certain effect in reducing cellular aging markers. Furthermore, its fermentation product filtrate can inhibit tyrosinase activity in vitro and significantly inhibit melanin production in a zebrafish model, showing potential as a tyrosinase inhibitor or melanin production inhibitor. Its fermentation product filtrate can also significantly inhibit the secretion of the cellular inflammatory factor NO, exhibiting good soothing effects and a certain ability to regulate the skin microecology.

The application discloses a low-temperature rotting-promoting composite microbial inoculum, a high-efficiency in-situ straw returning method and application, and belongs to the technical field of microbial inoculums. The low-temperature rotting-promoting composite microbial inoculum comprises Stenotrophomonas maltophilia JGDQ29-2, Bacillus JX-7, Weissella covesi zqw90, Weissella paramesenteroides zqw26 and Neurospora crassa N7, and the mass ratio of the viable bacterial numbers of the Stenotrophomonas maltophilia JGDQ29-2, the Bacillus JX-7, the Weissella covesi zqw90, the Weissella paramesenteroides zqw26 and the Neurospora crassa N7 is 1-2:1-2:1-2:1.5-2:2.5-3. The application realizes the synchronous high-efficiency degradation of each component of the straw, improves the overall rotting efficiency and improves the soil nutrient components by screening high-efficiency cellulose, hemicellulose and lignin degradation strains and constructing a composite microbial system with multiple bacteria symbiosis and function complementation.

Disclosed are universal LAB test kits, assays, apparatus and compositions for detecting and quantifying Lactobacillus, Pediococcus and Weissella bacteria, or combinations thereof that contaminate biological materials such as fermentation mashes for production of ethanol from a crop such as corn. The assays can be performed on-site by ethanol technicians having limited laboratory experience and are based on quantitative PCR (qPCR) methods. Primers and probes are described that specifically hybridize to a LAB bacteria gene. Also disclosed are sample preparation methods and apparatus, including a filtration syringe (100) that can increase sensitivity of the assays up to about 1000-fold compared to traditional sample preparation methods.

The invention discloses Weissella virida and application of Weissella virida in the aspects of hangover alleviating, inflammation resisting, aging resisting, lipid lowering and liver protecting. According to the invention, a Weissella viridescens strain is obtained through screening, the Weissella viridescens strain is named as Weissella viridescens NP12, and the Weissella viridescens strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 23, 2025 in No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.36310. The Weissella viridescens strain is named as Weissella viridescens strain is named as Weissella viridescens. The strain NP12 has multiple effects of dispelling the effects of alcohol, reducing lipid, protecting the liver, resisting inflammation, resisting aging, regulating intestinal flora and the like, and can also be used for fermenting medicinal and edible traditional Chinese medicinal materials such as radix puerariae, and the total polyphenol content, the anti-inflammation activity, the anti-aging activity and the liver protection activity of an obtained fermentation product are obviously superior to those of unfermented radix puerariae, so that the strain NP12 or the fermentation product thereof can be used for developing multifunctional foods or medicines.

The invention provides Weissella cibaria, a Weissella cibaria and mulberry leaf co-fermentation product and application of the Weissella cibaria and mulberry leaf co-fermentation product in sugar and lipid reduction. The Weissella sinus (Weissella sp.) BYQ07 obtained through separation is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36500, and the preservation date is November 4, 2025. The Weissella sinus (Weissella sp.) BYQ07 has the advantages that the Weissella sinus (Weissella sp. The bacterium has various potential biological functions and metabolic pathways, such as carbon metabolism, nitrogen metabolism, energy generation and conversion and the like. The invention further provides a weissella cibaria BYQ07 and mulberry leaf co-fermented product, and the weissella cibaria BYQ07 and mulberry leaf co-fermented product is obtained by co-fermenting weissella cibaria BYQ07 and mulberry leaves. Researches discover that Weissella cibaria BYQ07 and a co-fermentation product of Weissella cibaria BYQ07 and mulberry leaves have obvious effects in the aspects of reducing blood sugar, reducing fat deposition, improving insulin resistance, improving liver functions, relieving liver fatty degeneration and the like, so that the Weissella cibaria BYQ07 has obvious effects in the aspect of developing products for reducing blood sugar, reducing blood fat, improving insulin resistance and improving liver functions. The important application value is realized.

Aiming at the current situation that probiotics are used for treating periodontitis in the prior art, the invention provides a strain of mixed Weissella confusa LHJY. The mixed Weissella confusa is named as a mixed Weissella confusa LHJY strain, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20251930, and the preservation date is September 1, 2025. The method comprises the following steps: separating and purifying a pickled vegetable sample by an inventor to obtain probiotic mixed Weissella LHJY; a bacteriostatic experiment and a biological membrane removal experiment detect that the confused Weissella sp. LHJY has a remarkable inhibition effect on important periodontal pathogenic bacteria, namely porphyromonas gingivalis (P. gingivalis) and fusobacterium nucleatum (F. nucleatum), and fills up the blank of application of the confused Weissella sp. To treatment of periodontitis in the prior art, and the confused Weissella sp. LHJY has a remarkable inhibition effect on the periodontal pathogenic bacteria, namely porphyromonas gingivalis (P. gingivalis) and fusobacterium nucleatum (F. nucleatum). Moreover, the confused Weissella sp. LHJY can tolerate lysozyme with a relatively high concentration, effectively reduces the expression of inflammatory factors including interleukin 1 beta, tumor necrosis factor alpha and interleukin 6 in an inflammatory cell model, and has an important application prospect in the aspects of inhibiting periodontal disease pathogenic bacteria and regulating immune response.

In view of the current situation of probiotics for periodontitis treatment in the prior art, the application provides a Weissella confusa LHJY. The Weissella confusa LHJY strain is named as Weissella confusa LHJY strain, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M 20251930 and a preservation date of September 1, 2025. The inventors isolate and purify the probiotic Weissella confusa LHJY from a pickled vegetable sample; and find that the Weissella confusa LHJY has a significant inhibitory effect on P. gingivalis and F. nucleatum, which are important periodontal pathogenic bacteria, through a bacteriostatic experiment and a biofilm removal experiment, thereby filling the blank of application of the Weissella confusa to periodontitis treatment in the prior art. Moreover, the Weissella confusa LHJY can tolerate a high concentration of lysozyme, effectively reduces the expression of interleukin 1β, tumor necrosis factor α and interleukin 6 inflammatory factors in an inflammatory cell model, and has an important application prospect in inhibiting periodontal disease pathogenic bacteria and regulating immune responses.

This invention belongs to the field of yeast screening and application technology, and particularly relates to a strain of *Saccharomyces cerevisiae* HM-02 and its application in *Xanthoceras sorbifolium* malt-flavored baijiu. This invention uses HM-02 to ferment *Xanthoceras sorbifolium* cake, which significantly reduces the fat content of *Xanthoceras sorbifolium* and increases the antioxidant level and viable count of HM-02 in the fermentation broth. By adding *Xanthoceras sorbifolium* cake fermentation broth during the stacking stage of the brewing process, followed by fermentation in tanks, distillation, storage, and blending, *Xanthoceras sorbifolium* malt-flavored baijiu can be prepared. Results show that HM-02 in the *Xanthoceras sorbifolium* cake fermentation broth can survive in the mash, significantly increasing the abundance of *Saccharomyces cerevisiae* and *Weissella* species in the mash, decreasing the abundance of acid-tolerant lactic acid bacteria and *Saccharomyces cerevisiae*, increasing the content of aroma components such as β-phenylethanol in the *Xanthoceras sorbifolium* malt-flavored baijiu, increasing pleasant aroma components such as 2,3-butanediol and methyl heptanoate, and reducing the content of undesirable flavors.

The invention discloses fused Weissella sp. Capable of improving the performance of soluble dietary fiber prebiotics in bean dregs and application of the fused Weissella sp. Capable of improving the performance of soluble dietary fiber prebiotics in bean dregs, the fused Weissella sp. Is Weissella sp. Pro131 and has been preserved in Guangdong Microbiological Culture Collection Center on August 14, 2025, and the preservation number of the fused Weissella sp. Pro131 is GDMCC No.66842. The fusion Weissella sp. Has the breakthrough that bean dregs can be directly converted into a high-activity prebiotic product through single strain fermentation, and the function is excellent. The fused Weissella is used for fermentation modification of the bean dregs, the content of soluble dietary fibers (SDF) of the bean dregs is increased by 1.8-3.2 times, and the bean dregs are synchronously endowed with excellent physicochemical and functional characteristics: the water holding capacity is increased by 1.1-1.4 times, the oil holding capacity is increased by 10%-30%, and the cholesterol adsorption capacity in a simulated intestinal environment is increased by 4.5-4.8 times, so that the fermented bean dregs SDF shows a stronger probiotic effect; and a scarce strain resource and an efficient solution are provided for functional food development.