36 results about "Weissella" patented technology

The present invention is directed to a wound or tissue dressing comprising a bacteria having the property of producing lactic acid from sugars by fermentation of the sugars. The bacteria preferably belongs to the family of lactic acid bacteria. The family of lactic acid bacteria refers to any bacteria belonging to a genus selected from the group consisting of Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus and Weissella. There is also provided a wound or tissue dressing comprising an absorbent compound for absorbing wound exudate, wherein said wound or tissue dressing is attached to or comprises a lactic acid bacterium. The utility of the present invention is demonstrated by use of the wound or tissue dressings in methods for treating a wound or damaged tissue in an individual, said method comprising the steps of contacting said wound or damaged tissue with the wound or tissue dressing according to the invention, thereby treating the wound or damaged tissue. The treatment results in healing of the wound or in accelerated healing of the wound. There is also provided the use of a lactic acid bacteria in the manufacture of a wound or tissue dressing for treating or accelerating the healing of a wound in an individual.

The present invention is directed to a wound or tissue dressing comprising a bacteria having the property of producing lactic acid from sugars by fermentation of the sugars. The bacteria preferably belongs to the family of lactic acid bacteria. The family of lactic acid bacteria refers to any bacteria belonging to a genus selected from the group consisting of Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus and Weissella. There is also provided a wound or tissue dressing comprising an absorbent compound for absorbing wound exudate, wherein said wound or tissue dressing is attached to or comprises a lactic acid bacterium. The utility of the present invention is demonstrated by use of the wound or tissue dressings in methods for treating a wound or damaged tissue in an individual, said method comprising the steps of contacting said wound or damaged tissue with the wound or tissue dressing accordingto the invention, thereby treating the wound or damaged tissue. The treatment results in healing of the wound or in accelerated healing of the wound. There is also provided the use of a lactic acid bacteria in the manufacture of a wound or tissue dressing for treating or accelerating the healing of a wound in an individual.

The present invention relates to novel bacteria inhibiting halitosis or oral malodor. In particular, the present invention relates to novel lactic acid bacteria belonging to the genus Weissella, which can inhibit the proliferation of anaerobic bacteria producing volatile sulfur compounds by interacting with them and generating hydrogen peroxide under aerobic and anaerobic conditions. These lactic acid bacteria of the present invention are isolated from lactic acid bacteria naturally existing in the oral cavity of a person, and identified and deposited as Weissella cibaria CMU (Accession No.: KCTC 10650BP), Weissella cibaria CMS-1 (Accession No.: KCTC 10678BP), Weissella cibaria CMS-2 (Accession No.: KCTC 10679BP) and Weissella cibaria CMS-3 (Accession No.: KCTC 10680BP), respectively. The present invention provides the lactic acid bacteria and foods comprising the same for effectively inhibiting the proliferation of halitosis-inducing anaerobic bacteria without corrosion at teeth by using these Weissella cibaria lactic acid bacteria strains.

The present invention relates to novel bacteria inhibiting halitosis or oral malodor. In particular, the present invention relates to novel lactic acid bacteria belonging to the genus Weissella, which can inhibit the proliferation of anaerobic bacteria producing volatile sulfur compounds by interacting with them and generating hydrogen peroxide under aerobic and anaerobic conditions. These lactic acid bacteria of the present invention are isolated from lactic acid bacteria naturally existing in the oral cavity of a person, and identified and deposited as Weissella cibaria CMU (Accession No.: KCTC 10650BP), Weissella cibaria CMS-1 (Accession No.: KCTC 10678BP), Weissella cibaria CMS-2 (Accession No.: KCTC 10679BP) and Weissella cibaria CMS-3 (Accession No.: KCTC 10680BP), respectively. The present invention provides the lactic acid bacteria and foods comprising the same for effectively inhibiting the proliferation of halitosis-inducing anaerobic bacteria without corrosion at teeth by using these Weissella cibaria lactic acid bacteria strains.

The present invention provides a lactic acid bacterium culture containing protease-resistant bacteriocin, which can be produced by cultivating lactic acid bacteria (e.g., from the genus Weissella). As such, the shelf stability of a food can be improved by incorporating the culture of the present invention therein.

The invention discloses Weissella mesenteroides for promoting production of flavor substances in fermented food and application of the Weissella mesenteroides, and belongs to the field of microorganisms, the technical field of bioengineering and the field of food. The Weissella mesenteroides with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.22238, which is obtained by screening, comes from fermented grains for brewing Chinese liquor, has the properties of acid resistance, ethanol resistance, high temperature resistance and high salt resistance, and can adapt to the production environment of various foods; the strain has a gene for coding a plurality of substrate degrading enzymes, does not have a gene for coding an enzyme related to a biogenic amine or nitrite synthesis route, and is an excellent and safe brewing microorganism; when co-cultured with saccharomyces cerevisiae, Wickerhamomyces anomalus, lactobacillus acidophilus or brewage flavor flora, the strain can promote the flavor yield and the generation of substance types, and can be applied to pickles, enzymes, fermented meat products and other foods to improve the flavor characteristics of the fermented foods and reduce the production cost.

The invention discloses a Weissella paramestanoides and application thereof. The bacterial strain disclosed by the invention is Weissella paramestanoides with the preservation name of Weissella paramestanoides LL2018353; the bacterial strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is October 21, 2019; and the preservation number is CGMCC NO. 18710. The 16S rDNA gene sequence of the Weissella paramestanoides is a nucleotide sequence as shownin SEQ ID No. 1. The Weissella paramestanoides is strong in bacteriostasis and acidogenesis, can be preserved for a long time at a low temperature (4 DEG C), and can reduce the diarrhea rate and constipation rate of pigs.

The invention discloses a weissella viridescens loop-mediated isothermal gene amplification rapid detection kit and a detection method. The kit conducts large-scale genome analysis by means of a bioinformatic platform, four specific primers are designed according to a recN gene of weissella viridescens, an LAMP technology is combined, the rapid, sensitive and accurate detection method is established for the weissella viridescens, and the rapid detection kit used for the method is constructed. By means of the rapid detection kit and detection method, observing and identifying can be conducted with naked eyes after a reaction is conducted, other analysis steps of electrophoresis and the like are not needed, and the rapid detection kit and detection method have the advantages of being short in detection time, high in specificity, low in instrument and equipment requirement, easy and convenient to operate and the like and can be used for safe and rapid detection of samples of foods and environments, clinic samples and the like.

The invention discloses Weissella confuse YM5S2 with antibacterial and antioxidant activity and an application thereof. The Weissella confusa YM5S2 is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 07th, 2020 at the address of Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard No.1, Beichen West Road, Chaoyang District, Beijing, and the accession number is CGMCC No:19308. The Weissella confusa YM5S2 with antibacterial and antioxidant activity can inhibit growth and propagation of enteric pathogenic bacteria such as salmonella typhimuriumand the like in vitro, has no hemolytic property, is sensitive to most antibiotics, has high safety and has high survival rate in gastric acid and intestinal high-bile salt environments.

The invention discloses a Weissella fusion MP4 strain with an antibacterial effect and an application of the Weissella fusion MP4 strain. According to the invention, the MP4 strain with an inhibitingeffect on plant wilt caused by botrytis cinerea and fusarium oxysporum is obtained through screening; the strain is specifically classified and named as Wissella confuse MP4, is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M 2019701. According to the invention, experiments on botrytis cinerea and fusarium oxysporum spores, germ tube elongation and in vitro leaves are carried out; the MP4 strain has a high inhibition effect on botrytis cinerea and fusarium oxysporum, can be prepared into a biocontrol agent for preventing and treating botrytiscinerea and fusarium oxysporum, and has a good market application prospect.

The present invention pertains to a Weissella bacteria-derived vesicle and a use thereof. The present invention have experimentally confirmed that Weissella bacteria-derived vesicles efficiently inhibit inflammatory mediator secretion due to inflammation-inducing pathogenic vesicles, and it is thus expected that a Weissella bacteria-derived vesicle according to the present invention will be able to be usefully used for the purpose of developing a composition for preventing, alleviating, or treating inflammatory diseases.

The invention discloses a Weissella based high cadmium wastewater treatment method. The cadmium concentration in industrial wastewater can be reduced by using Weissella ZY-6. Industrial wastewater isintroduced into a sedimentation basin to perform precipitation, precipitated primary wastewater is conveyed to a filter tank provided with a biological filling material through a pipeline, and the Weissella ZY-6 grows in the biological filling material; and the initial sedimentation silt at the bottom of the sedimentation basin is conveyed to a silt treatment pool through a silt pump and a silt pipeline, bioleaching is performed on the initial sedimentation silt, and bioleaching bacteria liquid contains the Weissella ZY-6. The Weissella ZY-6 is used to perform treatment on high cadmium wastewater, the solid cadmium tolerance concentration of strains can reach up to 9,000 mg / L, and the liquid cadmium tolerance concentration of the strains can reach up to 900 mg / L, so that the strains are far superior to existing cadmium-tolerant strains; and solid-liquid separation and removal of cadmium can be performed on the wastewater, so that the method is strong in pertinency, and therefore, the removing rate of cadmium can be greatly enhanced, and biological costs can be saved.

The invention discloses a preparation method and application of a composite starter. The preparation method comprises the following steps of: step 1, establishing a culture medium; step 2, separating and identifying lactic acid bacteria; step 3, screening excellent strains; step 4, preparing a microbial agent; and step 5, preparing the composite starter. Weissella mesenteroides cooperates with lactobacillus plantarum and lactobacillus brevis to form the composite starter for fermenting leaf mustard, so that the flavor of a product is improved, and the composite starter has a better potential application value; by using the composite starter, the fermentation period of the leaf mustard can be shortened, the fermentation end point can be reached in advance, the harm of infectious microbes is reduced, the defects of the fermented leaf mustard in taste and flavor can be regulated and controlled, the use is convenient, and the flavor and health trend of food development are met; and the composite starter can be directly put into the production of the leaf mustard, so that the product quality is improved, the use is convenient, and the key problems of long fermentation period, poor product quality stability, poor flavor and the like in the processing and production of the leaf mustard are solved.

The invention provides a quantitative real-time fluorescence PCR (Polymerase Chain Reaction) quick detection kit of Weissella viridescens, and a detection method thereof. The quantitative real-time fluorescence PCR quick detection kit of the Weissella viridescens comprises reaction liquid 1 and reaction liquid 2, wherein the reaction liquid 1 comprises 10mmol / L of deoxyribonucleoside triphosphate,2.5mu L of 10*Taq Buffer reaction buffer solution, 1mu L of 20*SYBR Green I fluorochrome and 15.5 mu L of RNase-Free ddH2O; the reaction liquid 2 comprises 1.5mu L of 10mu mol / L upstream primer, 1.5mu L of 10 mu mol / L downstream primer and 5U / mu L Taq DNA (deoxyribonucleic acid) polymerase. The invention also specifically discloses a quantitative detection method and a qualitative detection method for the quantitative real-time fluorescence PCR quick detection kit of Weissella viridescens. Compared with a conventional PCR technology, the method has the advantages of high specificity, high sensitivity, simpleness in operation, high speed in detection, and accuracy in quantification, can be used for the quick detection of the Weissella viridescens, and has a high practical value.

The invention relates to a composite microecological preparation and application thereof in increasing intestinal short-chain fatty acid. The composite microecological preparation comprises the following components: xylooligosaccharide and lactic acid bacteria, wherein the lactic acid bacteria are one or more than two of lactobacillus brevis, enterococcus faecium, pediococcus acidilactici or weissella strains. The composite microecological preparation provided by the invention has the advantages that the content of cecum short-chain fatty acid is obviously improved, the contents of acetic acid, propionic acid and butyric acid are improved, the content of acetic acid is improved to the most obvious, the probiotic effect is better exerted, and the intestinal health is improved.

The invention discloses Weissella for promoting zygosaccharomyces rouxii to produce guaiacol substances, and belongs to the technical field of biological engineering. By co-culturing Weissella paramesenteroides LCW-28 and zygosaccharomyces rouxii ZQ-01 in a culture medium, the yield of guaiacol substances is effectively increased; the total yield of the guaiacol substances is the highest; the total yield of the guaiacol substances is increased by 3.87 times compared with the total yield of the guaiacol substances cultured independently, is increased by 15.71 times compared with candida, and is increased by 17.28 times compared with torulopsis glabrata, wherein the yield of 4-ethyl guaiacol is 2.12 mg / L, and is 8.8 times of that of the single culture of the zygosaccharomyces rouxii.

The present invention relates to prediction of preterm birth by using a change in blood microbiome and, more particularly, provides a composition for diagnosing a preterm birth risk by detecting strains of Weissella spp., Thermacetogenium spp., Faecalibacterium spp., Clostridium spp., or Lactobacillus spp. in the blood of pregnant women.

The invention relates to a composite microecological preparation and its application in improving intestinal short-chain fatty acids. The components of the composite microecological preparation include: xylooligosaccharides and lactic acid bacteria; the lactic acid bacteria are: Lactobacillus pumilus, intestinal One or more of coccus, Pediococcus lactis or Weissella species; the composite microecological preparation of the present invention can significantly increase the content of short-chain fatty acids in the cecum, and increase the content of acetic acid, propionic acid, and butyric acid , the most significant increase in acetic acid content, better play a probiotic role, and improve intestinal health.

Disclosed are universal LAB test kits, assays, apparatus and compositions for detecting and quantifying Lactobacillus, Pediococcus and Weissella bacteria, or combinations thereof that contaminate biological materials such as fermentation mashes for production of ethanol from a crop such as corn. The assays can be performed on-site by ethanol technicians having limited laboratory experience and are based on quantitative PCR (qPCR) methods. Primers and probes are described that specifically hybridize to a LAB bacteria gene. Also disclosed are sample preparation methods and apparatus, including a filtration syringe (100) that can increase sensitivity of the assays up to about 1000-fold compared to traditional sample preparation methods.

The invention discloses a Weissella cibaria and an application, screening and testing method thereof. The Weissella cibaria is preserved in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee, and the preservation number is CGMCC No. 15075; The Weissia sinensis has a fast growth rate and a high acid-producing efficiency; the Weissia antrophagum is used as a forage silage additive to improve the quality of forage silage, and has a wide application prospect.

The invention discloses Weissella sinus with broad-spectrum antibacterial activity, and belongs to the field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20211696, the preservation address is Wuhan University, Wuhan, China, and the preservation date is December 29, 2021. The Weissella cibaria BYL4.2 disclosed by the invention is separated from pickles, and experiments prove that the Weissella cibaria BYL4.2 can inhibit growth and reproduction of penicillium chrysogenum, aspergillus versicolor, escherichia coli, staphylococcus aureus, listeria monocytogenes and pseudomonas aeruginosa in vitro, and has broad-spectrum antifungal and bacterial activity; moreover, the compound is sensitive to antibiotics such as erythromycin and rifampicin, is relatively high in safety, is high in survival rate in gastric acid and intestinal high-bile-salt environments, and has potential application prospects in the fields of improvement of the intestinal environment and preservation and fresh keeping of foods.

The invention relates to a pickle fermentation compound bacteria and a preparation method thereof. The preparation method comprises the following steps: firstly, selecting and activating leuconostoc viride, lactobacillus brevis and Weissella bursa-pastoris, carrying out enlarged culture in an MRS broth culture medium, carrying out centrifugal resuspension, measuring the concentration of bacterialliquid, and mixing according to a ratio of 1: 1: 1 to prepare compound bacteria; cleaning, draining and cutting fresh green vegetable heads into blocks with the same size, adding 4% of edible salt, 3%of rock candy, 4% of ginger, 5% of garlic, 5% of chilli, 0.5% of Chinese prickly ash and a mixed bacterial solution, wherein the ratio of the green vegetable heads to water is 1: 2; and finally, adding compound bacteria according to the amount of 3mL / L, sealing, and fermenting at room temperature for 7 days to obtain the fermented mature pickle. The pickle prepared by the preparation method disclosed by the invention is fragrant and crisp in taste, low in nitrite content, low in titratable acid content, good in palatability and high in health degree.

The invention discloses a Weissella viridescens rapid detection method based on droplet digital PCR. The method comprises the following steps: firstly, extracting genome DNA of to-be-detected sample enrichment broth, and performing diluting for later use; designing a specific primer and a probe by utilizing a conservative DNA sequence of a Weissella viridescens genome; then carrying out droplet digital PCR amplification and detection on the genome DNA of the to-be-detected sample enrichment broth by using the primer and the probe; after the PCR amplification, detecting each droplet one by oneby using a droplet analyzer, wherein the droplet with a fluorescence signal is interpreted as 1, the droplet without the fluorescence signal is interpreted as 0; and finally, calculating the concentration or copy number of to-be-detected target molecules according to the Poisson distribution principle and the proportion of positive droplets. The number of DNA molecules can be directly obtained through droplet digital PCR, and absolute quantification can be conducted on Weissella viridescens in a sample. The method has the advantages of high sensitivity, accurate quantification, wide detectionlinear range, good specificity and moderate cost.

The invention discloses a fused Weissella MP4 strain with antibacterial effect and its application. The present invention obtains the MP4 strain with inhibitory effect on plant wilt caused by Botrytis cinerea and Fusarium oxysporum through screening. The specific taxonomy named fusion Weissella MP4 Weissella confuse MP4, preserved in China Center for Type Culture Collection, its preservation number is CCTCC NO: M 2019701. The present invention proves that the MP4 bacterial strain obtained by the present invention has a stronger inhibitory effect on Botrytis cinerea and Fusarium oxysporum through experiments on Botrytis cinerea and Fusarium oxysporum spores, germ tube elongation and isolated leaves, and can It is prepared as a biocontrol agent for preventing and treating Botrytis cinerea and Fusarium wilt, which has a good market application prospect.

The invention relates to an SsDNA aptamer capable of specifically recognizing Weissella viridescens as well as a screening method and application of the ssDNA aptamer. The screening method comprises the following steps: constructing a random library and a primer pair, taking the Weissella viridescens as a forward screening strain and taking lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides, pediococcus pentosaceus and staphylococcus aureus as inverse screening strains, excluding sequences with low specificity, excluding non-specifically bound sequences through bovine serum albumin and tRNA, and obtaining an ssDNA library through screening; and sequencing the ssDNA library obtained by screening, selecting a sequence with a stable structure and relatively low free energy, and then screening out a sequence with high affinity and high specificity to the Weissella viridescens, so as to obtain the ssDNA aptamer capable of specifically recognizing the Weissella viridescens. The ssDNA aptamer has a potential application prospect in the aspect of accurately, quickly and sensitively detecting the Weissella viridescens.

The invention discloses a strain of Weissia bacterium that promotes the production of guaiacol by Zygomyces ruckeri, belonging to the technical field of bioengineering. The production of guaiacols was effectively improved by co-cultivating Weissella enteritidis LCW‑28 and Zygosaccharomyces rouckeri ZQ‑01 in the medium, and the total production of guaiacols was the highest compared with the single culture Increased by 3.87 times, compared with Candida, increased by 15.71 times, and compared with Toruula glabrata, increased by 17.28 times; wherein, the 4-ethyl guaiacol output was 2.12mg / L, which is the only method for culturing Lushi 8.8 times that of Zygomyces.

The invention discloses a strain of Weissella enterica-like and its application. A strain of Weissella enteroides of the present invention, the strain is Weissella paramesenteroides; the preservation name is Weissella enteritidis LL2018353; it is preserved in China Microorganism Culture Preservation Management Committee Common Microorganisms Center (CGMCC), the deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing; date of deposit: October 21, 2019; deposit number: CGMCC NO.18710. The 16S rDNA gene sequence of Weissella enteroides is the nucleotide sequence shown in SEQ ID No.1. The invention has strong bacteriostasis and acid production, can be stored at low temperature (4 degrees Celsius) for a long time, and can reduce the diarrhea rate and constipation rate of pigs.

The invention belongs to the technical field of microorganisms, and discloses weak post-acidification Weissella fusiformis and application thereof. The weak post-acidification Weissella fusiformis PCYLJY-1 provided by the invention is suitable for preparing weak post-acidification fermented fruit and vegetable products, while a weak post-acidification strain in the prior art is only suitable for solving the post-acidification problem of fermented milk, and the strain not only has a remarkable weak post-acidification effect, but also has excellent fermentation quality; and the weak post-acidification Weissella fusiformis has huge application potential in solving the post-acidification problem of fermented fruit and vegetable products. The weak post-acidification Weissella fusiformis PCYLJY-1 provided by the invention is a natural weak post-acidification strain screened by evaluating the acidification characteristics of the strain, and the weak post-acidification performance is stable.

The invention discloses dominant flora in a white gourd cooked curing process and an amplification primer thereof. In the preliminary stage of white gourd cooked curing, dominant bacteria in the curing system include acinetobacter, Weissella and bacillus; in the early stage, the dominant bacteria include Weissella, bacillus and enterobacter; in the middle stage, the dominant bacteria include lactobacillus, bacillus and Weissella; in the later stage, the dominant bacteria include Weissella, bacillus and staphylococcus; the upstream and downstream primers of the similar leuconostoc mesenteroides are shown by SEQ ID No.1 and No.2; the upstream and downstream primers of acinetobacter calcoaceticus are shown by SEQ ID No.3 and No.4; the upstream and downstream primers of Weissella cibarium are shown by SEQ ID No.5 and No.6; and the upstream and downstream primers of lactobacillus curvatus are shown by SEQ ID No.7 and No.8. According to the invention, the dynamic change conditions of the dominant microorganisms in a white gourd fermentation process are revealed so as to provide theoretical basis to the technological improvement and quality control.