Complete medium and human amnion-derived mesenchymal stem cell culture method

A technology of complete medium and culture method, applied in the field of culture of complete medium and human amniotic mesenchymal stem cells
CN102191218AActive Publication Date: 2011-09-21AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
Publication Date
2011-09-21

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Abstract

The invention discloses a complete medium and a human amnion-derived mesenchymal stem cell (hAMSCs) culture method. The complete medium is prepared by adding 3 to 10 percent of autologous umbilical cord blood serum into low-sugar Dulbecco minimum essential medium solution according to a volume ratio. The culture method comprises: (1) separation; (2) primary culture; and (3) subculture. The methodusing the complete medium in the hAMSCs culture has the advantages that: the risk of using fetal calf serum is avoided; although the need of adding L-glutamine, non-essential amino acid, 2-mercapitoethanol, pyruvic acid and the like is obviated, the high proliferation properties and phenotypic characteristics of the hAMSCs and expression of multilineage differentiation marker genes sand proteins of some stem cells can still be retained; and in subculture, the wall adherence fastness of the hAMSCs is much lower than that in fetal bovine serum (FBS) culture, the digestion time is reduced obviously, and the damage of trypsinization to cells and loss of cells are reduced.
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Description

technical field

[0001] The invention relates to a complete culture medium and a culture method of human amniotic mesenchymal stem cells (human amniotic mesenchymalstem cells, hAMSCs), belonging to the technical fields of cell engineering and biomedicine. Background technique

[0002] Human amnion is a layer of translucent film attached to the surface of the placental chorion, which is a part of the fetal membrane tissue and evolved from the cytotrophoblast during embryonic development. In addition to human amniotic epithelial cells, hAMSCs are one of the main cell types constituting human amniotic membrane tissue, express embryonic stem cells and mesenchymal stem cells (mesenchymal stem cells, MSCs) markers, and have multi-lineage differentiation potential. MSCs. Under specific induction conditions in vitro, hAMSCs can differentiate into all cells from the three germ layers, including nerve cells, cardiomyocytes, pancreatic cells, and liver cells. Due to its multi-directio...

Claims

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