Preparation for promoting fibroblasts to secrete extracellular matrix components and preparation method thereof

A technology of fibroblasts and extracellular matrix, applied in the direction of medical preparations containing active ingredients, peptide/protein ingredients, skin care preparations, etc., can solve the problem of not maintaining the balance of extracellular matrix and improving extracellular matrix, There is no in-depth research and the addition of ECM compound components to achieve the effects of preventing and treating thinning hair, maintaining balance, and improving biological safety

Inactive Publication Date: 2010-02-17
BEIJING ZHONGKE YINUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, increasing a certain matrix component secreted by cells at a single level cannot maintain the balance of the extracellular matrix and improve the state of the extracellular matrix. There is no in-depth study on how to increase the secretion of ECM complex components at the same time.

Method used

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  • Preparation for promoting fibroblasts to secrete extracellular matrix components and preparation method thereof
  • Preparation for promoting fibroblasts to secrete extracellular matrix components and preparation method thereof
  • Preparation for promoting fibroblasts to secrete extracellular matrix components and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. Preparation and RT-PCR detection of preparations for promoting fibroblasts to secrete extracellular matrix components

[0018] Take the egg white of fresh eggs (Bailaihang, Shouguang or Bailock) and let it stand overnight at 4°C, centrifuge at 4000r / min for 10min to get 2mg of the supernatant, dilute it with ultrapure water at a volume ratio of 1:10, and add 200mg / ml of egg white lysozyme and 20 mg / ml of sodium pyruvate were mixed to obtain the preparation for promoting fibroblasts to secrete extracellular matrix components provided by the present invention.

[0019] Take a Kunming white mouse with a gestational age of 14.5 days, remove the limbs and viscera, take the back skin, and cut it into pieces.

[0020] Soak 600U / ml collagenase and 400U / ml hyaluronidase overnight at 4°C, digest at 37°C for 1 hour, pass through a 200-mesh cell sieve to obtain mouse skin fibroblast suspension, add 10% fetal bovine serum to DMEM medium, and pass passage After purificat...

Embodiment 2

[0035] Example 2. Preparation of a preparation for promoting fibroblasts to secrete extracellular matrix components and the effect of the preparation on the content of hydroxyproline in mouse skin fibroblasts

[0036] Take the egg white of fresh eggs (both Bailaihang, Shouguang and Bailock) and let it stand overnight at 4°C, centrifuge at 4000r / min for 10min to get 4mg of the supernatant, dilute it with ultrapure water at a volume ratio of 1:10, and add 400mg / ml of egg white lysozyme and 5 mg / ml of sodium pyruvate were mixed to obtain the preparation provided by the invention for promoting the secretion of extracellular matrix components by fibroblasts.

[0037] Take a Kunming white mouse with a gestational age of 14.5 days, remove the limbs and viscera, take the back skin, and cut it into pieces.

[0038] Soak 600U / ml collagenase and 400U / ml hyaluronidase overnight at 4°C, digest at 37°C for 1 hour, pass through a 200-mesh cell sieve to obtain mouse skin fibroblast suspensio...

Embodiment 3

[0042] Example 3. Preparation of a preparation for promoting the secretion of extracellular matrix components by fibroblasts and the effect of the preparation on the content of hydroxyproline in human skin fibroblasts

[0043] Take fresh eggs (Bailaihang, Shouguang or Bailock can all be used), take the egg white and let it stand overnight at 4°C, centrifuge at 4000r / min for 10min to get 0.75mg of the supernatant, dilute it with ultrapure water at a volume ratio of 1:10, and add 15mg Egg white lysozyme / ml and 0.2 mg / ml sodium pyruvate were mixed to obtain the preparation for promoting fibroblasts to secrete extracellular matrix components provided by the present invention.

[0044] Culture of human skin fibroblasts: DMEM medium, add 10% fetal bovine serum, add 5% of the preparation obtained by the above preparation method in the medium, as the preparation group; in the control group, the culture medium is DMEM medium, add 10% Fetal bovine serum, culture medium was changed every...

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Abstract

The invention discloses a preparation for promoting fibroblasts to secrete extracellular matrix components, and a preparation method and application thereof. The preparation comprises the components of A) the egg white of eggs, B) egg white lysozyme, and C) pyruvic acid or pyruvate, can also comprise ovotransferrin and/or glucose, and is obtained by mixing the components uniformly. The preparationcan serve as an externally used paste for skin in application, and can obviously stimulate the secretion of collagen and hyaluronic acid synthase in the fibroblasts of the skin and improve the resiliency and the moisture content of the skin. The preparation is derived from a biologic extractive technique, has no harmful substance residues, safe and high-efficiency use, and is different from the prior method for promoting the secretion of a single fibroblast ECM component; besides, the preparation has multiple effects, wide application range and extensive application prospect, and can be applied in the fields of the preparation of skin substitutes, the preparation of anti-aging skin care products, cosmetics, medical treatment and the like.

Description

technical field [0001] The invention relates to a preparation for promoting fibroblasts to secrete extracellular matrix components, a preparation method and application thereof. Background technique [0002] The extracellular matrix (ECM), including collagen, noncollagenous glycoproteins, aminoglycans and proteoglycans, and elastin, maintains tissue function by maintaining cell survival, determining cell shape, regulating cell proliferation, controlling cell differentiation, and participating in cell migration Normal, maintain a strong metabolism, so as to maintain the youthful state of the body. Fibroblasts are involved in the secretion of various extracellular matrix components, including the regulation of the secretion of collagen, fibronectin, hyaluronic acid, chondroitin sulfate, dermatan sulfate, and elastin. [0003] Fibroblasts are also the main cell components in the skin dermis. The collagen fibers, elastic fibers and matrix components they secrete together with f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/47A61K9/06A61K8/98A61P17/00A61Q19/08A61Q19/00A61K35/54A61K38/40A61K31/7004A61K31/19A61K38/43A61K38/39A61L27/60A61K35/57
Inventor 连正兴韩红兵李想于坤
Owner BEIJING ZHONGKE YINUO BIOTECH
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