68 results about "Extracellular matrix component" patented technology

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

A three-dimensional mammary gland model comprising a three-dimensional tissue comprising cells and fragmented extracellular matrix components, the cells comprising mature adipocytes and mammary gland epithelial cells.

The present invention relates to peptides having skin condition improving activity. The peptide of the present invention increases the expression level of an extracellular matrix component, again reduces the expression of a skin cell injury factor increased due to ultraviolet radiation, and again restores the expression level of the extracellular matrix component reduced due to ultraviolet radiation.

The present disclosure pertains to a method for producing a three-dimensional mammary gland model that comprises a three-dimensional tissue body including mature adipocytes and mammary epithelial cells, the method comprising: a step for gelling a mixture that contains a fragmented extracellular matrix component, an alginate gel precursor, and cells that include adipose-derived stem cells and mammary epithelial cells; and a step for subjecting the gelled mixture to differentiation induction culture to thereby differentiate at least a portion of the adipose-derived stem cells into the mature adipocytes to form the three-dimensional tissue body.

The invention belongs to the technical field of biomedicine, and particularly relates to hydrogel for evaluating corneal endothelium seed cell functions and a preparation method and application thereof.The hydrogel is of a three-dimensional cross-linked network structure formed by acrylamide in the presence of N, N '-methylene bisacrylamide through free radical polymerization; meanwhile, an extracellular matrix component is fixed on the surface of the three-dimensional cross-linked network structure, and the hydrogel is obtained. According to the hydrogel prepared by the method, the problems of mismatching of mechanical properties, large batch difference and the like of a traditional material are solved, and a more reliable standardized tool is provided for HCE cell behavior research. The breakthrough technology solves the problem that a traditional material cannot give consideration to both physical support and function evaluation.

The application discloses a Mycoplasma bovum adhesion protein FtsZ and application thereof. The nucleic acid sequence of the Mycoplasma bovum adhesion protein FtsZ is shown as SEQ ID NO. 1. The application also discloses a recombinant plasmid pET-30a-ftsZ and an E. coli containing the recombinant plasmid pET-30a-ftsZ. ftsZ The recombinant protein rFtsZ has the advantages of being capable of specifically combining with EBL cell membrane protein, being capable of combining with extracellular matrix components (fibronectin, fibronectin, laminin and type IV collagen), having the direct adhesion host cell effect, having good antigenicity, being capable of producing high-level antibodies, being capable of providing good immune protection effect, and providing a new target and thought for elucidating the pathogenic mechanism of the Mycoplasma bovum and developing a new vaccine and medicine.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

A clinical combination therapy comprising one or more pharmaceutical compositions for treating various cancers. The one or more pharmaceutical compositions include at least a first composition of an immune checkpoint inhibitor and a second composition that interacts with an extracellular matrix component of the tumor microenvironment. The first composition may, for example, include pembrolizumab, and the second composition may include an LAIR-2Fc fusion protein, such as NC410. The compositions follow a dosing and timing regimen.

To provide a preparation effective in promoting the production of any one of collagen, elastin, or hyaluronic acid, which are extracellular matrix components, or to provide a preparation effective in promoting epithelial wound healing.SOLUTION: Provided are: a composition for promoting the production of extracellular matrix components, the composition containing extracellular vesicles derived from bacteria and / or fungi, and the extracellular matrix components being at least one selected from the group consisting of collagen, elastin and hyaluronic acid; or a composition for promoting the expression of COL1A1, COL3A1, ELN, HAS1 or HAS2 genes; a composition for improving the physical properties or appearance of skin, eyeball or connective tissue; and a composition for promoting epithelial wound healing, containing extracellular vesicles derived from bacteria and / or fungi.SELECTED DRAWING: None

The invention belongs to the technical field of biology, and particularly relates to a nucleus pulposus cell separation and culture method. According to the method, a step-by-step enzyme digestion method is adopted, NB6 collagenase is firstly used for primary digestion, and then compound enzyme liquid composed of trypsin and hyaluronidase is adopted for subsequent digestion; and culturing in combination with a culture vessel coated with a specific concentration of extracellular matrix component and a special culture medium. According to the method, on the premise of not depending on an immortalization technology, the primary nucleus pulposus cells with good activity can be efficiently obtained, stable amplification and long-term passage of the primary nucleus pulposus cells in vitro are realized, and specific marker expression and biological functions of the nucleus pulposus cells can be effectively maintained. The invention provides a stable cell source and technical platform for the development of cell therapy products and the research of spinal degenerative diseases.

The present invention relates to a dispersion comprising acellular dermal matrix microparticles, a filler comprising the same, and uses thereof. More specifically, the present invention relates to a filler comprising a dispersion containing acellular dermal matrix microparticles obtained by supercritical fluid extraction and high pressure dispersion operations, and a method for preparing the same. The acellular dermal filler according to the present invention has excellent biological stability and biocompatibility, and extracellular matrix components such as collagen and elastin are significantly preserved therein. In addition, the filling agent also shows excellent biochemical stability, the pH value or the collagen content and the elastin content are kept unchanged in the storage process, and the filling agent also shows physical stability and can continuously maintain injectability. Therefore, the acellular dermal filler can be effectively used as an injectable filler for repair or volume expansion of soft tissue and reduction of wrinkles in the field of plastic and regenerative medicine.

The present invention discloses the application of hydrolyzed aloe flower protein in skin repair products. The amino acid sequence of the hydrolyzed aloe flower protein is at least one of Leu-Pro-Phe, Leu-Gly, and Ala-Phe. The hydrolyzed aloe flower protein of the present invention improves dermal homeostasis, especially the functional homeostasis of dermal fibroblasts, by enhancing the proliferation and migration abilities of dermal fibroblasts, promoting the synthesis of extracellular matrix components of dermal fibroblasts, and releasing fibroblast growth factors, thereby achieving the purpose of skin repair.

Microfluidics technology incorporating 3D gel droplets is provided for analysis of tumor cells and their interactions with immune cells, such as natural killer cells. The gel droplets include the presence of extracellular matrix components, which alter cell functions including viability and mobility. The system allows cells co-incorporated into 3D gel droplets to be observed in real-time using fluorescence microscopy, with fluorescence-based sorting of droplets for recovery of individual cells based on their function. High-quality RNA can be extracted from the sorted droplets for transcription profiling.

A three-dimensional mammary gland model includes a three-dimensional tissue containing cells and fragmented extracellular matrix components, and the cells include mature adipocytes and mammary epithelial cells.

The invention discloses a low-energy photocuring ophthalmic sealant and a preparation method and application thereof.The ophthalmic sealant is in-situ photocuring bi-crosslinking biological hydrogel, a functional complementary composite system is constructed through collaborative optimization, and the ophthalmic sealant mainly comprises methacrylated gelatin (GelMA), a cornea extracellular matrix component, glutamine transaminase and a photoinitiator. According to the invention, a double-network construction strategy combining low-energy photocuring and enzymatic crosslinking is adopted, and curing can be triggered under the extremely low optical power density not higher than 5 mW / cm, so that the clinical application bottlenecks of tissue damage caused by too high energy of a traditional photocuring material for eyes and insufficient mechanical property and stability of a single crosslinking system are overcome. The sealing agent has excellent tissue adhesion, mechanical stability and biocompatibility in a double-crosslinking hydrogel state, can realize minimally invasive sutureless repair of corneal defects, and effectively promotes functional regeneration of corneal tissues.

The present invention relates to a biocomposite, regenerative, high-strength ligament graft, the ligament graft being woven from at least four types of ligament fiber bundles; the ligament fiber bundles being woven from different proportions of porcine small intestinal submucosal fibers and composite fibers; wherein the porcine small intestinal submucosal fibers are made by curling the porcine small intestinal submucosal layer; and the composite fibers are made by wrapping the porcine small intestinal submucosal layer around the surface of the high-strength fibers. The ligament graft of the present invention is woven from porcine small intestinal submucosal fibers and porcine small intestinal submucosal composite high-strength fibers. The porcine small intestinal submucosal layer retains extracellular matrix components and microstructures, facilitating cell migration, adhesion, and proliferation. The composite high-strength fibers are prepared by combining the composite high-strength fibers with a degradable polymer material, which serves as a mechanical support component. The complementary advantages of the degradable polymer material give the ligament graft of the present invention both good biocompatibility and strength.

Injectable compositions and use of the injectable compositions in tissue engineering applications are described. The injectable compositions are hydrogel-based compositions that can be crosslinked in situ following placement. The injectable compositions include microcapsules having predetermined erosion profiles that are loaded with nanogels having predetermined sustained release profiles for signaling molecules conjugated to the nanogels. Following crosslinking, the compositions are designed to sequentially release signaling molecules over a predetermined period of time with various release profiles. The compositions can carry additional components to stimulate tissue generation such as stem cells and extracellular matrix (ECM) components.

The present invention relates to peptides having skin condition improving activity. The peptide of the present invention increases the expression level of an extracellular matrix component and a skin barrier enhancing factor, reduces the expression of a skin cell injury factor increased due to ultraviolet radiation, and restores the expression level of an extracellular matrix component and a skin barrier enhancing factor reduced due to ultraviolet radiation.

Novel methods of creating personalized mesenchymal stem cells from pluripotent stem cells, including the utilization of extracellular matrix components, optionally seeded with exogenous differentiation factors for creation of a substrate for promotion of mesenchymal stem cell formation from pluripotent cell populations. Matrices useful for stimulation of differentiation include decellularized bone matrix, placental matrix, and adipose tissue derived matrix. Growth factors such as BMP2, BMP4, GDF-11, and stem cell factor can be added to said matrix. Stimulation of differentiation can be induced by exposure to conditioned media isolated from mesenchymal stem cells.

The invention belongs to the technical field of biology, and particularly relates to an embryo biopsy culture solution, an embryo biopsy method and application of the embryo biopsy culture solution. A polypeptide growth factor; a signal path modulator; wherein the signal channel regulator comprises an ROCK signal channel inhibitor and / or an MLCK signal channel inhibitor. According to the biopsy culture solution disclosed by the invention, all the components can efficiently and synergistically act on an embryo biopsy microenvironment through careful screening and optimized combination and collocation; further, the physiological environment of the embryo can be accurately simulated, the integrity and stability of the morphological structure of the embryo are maintained, the operation damage of embryo biopsy is remarkably reduced, key nutrition support and anti-apoptosis protection are provided, and the development potential and transplantation conception rate of the embryo are maintained; the invention effectively solves the core problems of poor embryo survival ability, low re-cavity rate, impaired developmental potential, low transplantation conception rate and the like caused by embryo biopsy in the prior art, provides a standardized, efficient, stable and safe special culture solution for early embryo biopsy operation of mammals, and is highly compatible with a genetics detection technology. The application prospect is wide.

Provided are preparation methods for an islet extracellular matrix scaffold and a biomimetic islet organoid. The scaffold is a gel, and the gel contains 3-5 mg / mL Collagen I, 3-5 mg / mL Collagen IV, and 0.8-1.2 mg / mL Laminin. For the scaffold, Collagen I, Collagen IV, and Laminin are simultaneously selected as extracellular matrix components, which have relatively strong interactions with islet cells, and effectively promote the islet cells and enhance insulin secretion function stimulated by glucose after binding to the islet cells. Collagen VI can also be used. Collagen VI can act on the islet cells to effectively enhance the survival of the islet cells and the insulin secretion function stimulated by glucose. The gel can form a relatively intact network structure without needing to use a cross-linking agent, thereby enhancing the support capacity for the islet organoid.

The application discloses a multifunctional hydrogel patch, a preparation method and application thereof, and belongs to the field of tissue engineering and regenerative medicine. The biological patch comprises a decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs). The dFCT is obtained by decellularization and freeze-drying treatment of tumor tissue, and retains abundant natural extracellular matrix components. The HNPs are extracted from hair and have excellent active oxygen scavenging capacity and photothermal conversion performance. The hydrogel layer is preferably methacrylated gelatin (GelMA). The patch shows significant cell activity promotion and antibacterial capacity in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch has the advantages of simple preparation process, natural component source, multiple functions such as antibacterial, antioxidant and tissue regeneration promotion, and is suitable for wound repair treatment.

The invention relates to a medicine for inhibiting breast cancer angiogenesis, and discloses an application of genipin in preparation of the medicine for inhibiting breast cancer angiogenesis. Research results of the invention show that the genipin can directly inhibit expression of HIF-1alpha so as to interfere hypoxia-related pathways and prevent hypoxia-related transcription factors from playing a role. In addition, the genipin can also reduce the expression of HSPG2, so that the accumulation of extracellular matrix components is reduced, and cell proliferation, migration and angiogenesis damage are finally caused, and therefore, the genipin can be used for preparing the medicine for inhibiting breast cancer angiogenesis.

To provide a gel composition that is transparent and has a high modulus of elasticity, containing extracellular matrix components and / or fragmented extracellular matrix components. [Solution] A gel composition containing at least one selected from the group consisting of extracellular matrix components and fragmented extracellular matrix components, and ions of a metal element.

Compositions containing an anesthetic and an extracellular matrix component or fragment thereof and methods for treating pain by topically administering such compositions are described herein.

The application discloses a preparation method of umbilical cord decellularized matrix based on perfusion decellularization technology, which comprises decellularization treatment of a complete umbilical cord by using a programmed perfusion method, and cleaning, enzymatic hydrolysis, freeze-drying and other processes. The application further discloses the umbilical cord decellularized matrix prepared by the method and application thereof. The obtained umbilical cord decellularized matrix has complete structural integrity, retains not only umbilical cord Wharton's jelly but also rich low-immunogenic extracellular matrix components in umbilical cord tissues, and has wide application prospects in the development of soft tissue injury repair, medical beauty and life beauty related products.

Provided herein are methods of treating cancer comprising utilization of tumor infiltrating lymphocytes expressing tumor-targeted and membrane-anchored IL-12. Also provided herein are methods of treating cancer comprising utilization of T cells expressing tumor-targeted and membrane-anchored IL-12 with an additional therapeutic agent, particularly a therapeutic agent that inhibits CCKAR and / or CCKAR signaling pathways.

The invention provides a biological tissue decellularization method and a biological tissue, and relates to the technical field of biological materials. The biological tissue decellularization method provided by the invention comprises the following steps: soaking a disinfected target biological tissue in a degreasing solution to obtain a degreased target biological tissue, the degreasing solution comprising a detergent, and the degreasing solution further comprising at least one of a polyol reagent and an extracellular matrix component; and soaking the degreased target biological tissue with a pancreatin solution to obtain the decellularized target biological tissue.

The present invention relates to a dispersion comprising acellular dermal matrix microparticles, a film comprising same, and uses thereof. More specifically, the present invention relates to a film containing a dispersion comprising acellular dermal matrix microparticles obtained through supercritical fluid extraction and high-pressure dispersion operations, and a manufacturing method therefor. The acellular dermal film according to the present invention exhibits excellent biostability and biocompatibility, and demonstrates significantly superior preservation of extracellular matrix components such as collagen. In addition, the present invention has excellent mechanical properties and thus can be easily applied to graft surgery and suturing, and has a porous structure that facilitates the movement of body fluids, and thus may support tissue regeneration. Accordingly, the acellular dermal film can be usefully employed in the field of regenerative medicine as as wound dressings, adhesion barriers, and as implantable biomaterials such as drug delivery implants and artificial skin.

The invention discloses a perfusion decellularization technology-based preparation method of an umbilical cord decellularization matrix, which comprises the following steps: carrying out decellularization treatment on a complete umbilical cord by using a programmed perfusion method, cleaning, carrying out enzymolysis, freeze-drying and the like, and also discloses the umbilical cord decellularization matrix prepared by the method and application thereof. The obtained umbilical cord acellular matrix has structural integrity, not only retains umbilical cord Wharton jelly, but also retains rich low-immunogenicity extracellular matrix components in umbilical cord tissues, and has wide application prospects in development of soft tissue injury repair, medical cosmetology and life cosmetology related products.