28 results about "Extracellular matrix component" patented technology

The invention discloses a multifunctional hydrogel patch and a preparation method and application thereof.The biological patch comprises decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs), the dFCT is obtained by decellularizing and freeze-drying the tumor tissue, and rich natural extracellular matrix components are reserved; hNPs is extracted from hair and has excellent active oxygen scavenging capacity and photothermal conversion performance; it is preferable that the hydrogel layer is a methacrylated gelatin (GelMA). The patch disclosed by the invention shows a remarkable cell viability promoting effect and antibacterial ability in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch is simple and convenient in preparation process and natural in component source, has multiple functions of resisting bacteria, resisting oxidation, promoting tissue regeneration and the like, and is suitable for wound repair treatment.

The present invention relates to peptides having skin condition improving activity. The peptide of the present invention increases the expression level of an extracellular matrix component, again reduces the expression of a skin cell injury factor increased due to ultraviolet radiation, and again restores the expression level of the extracellular matrix component reduced due to ultraviolet radiation.

The application discloses a Mycoplasma bovum adhesion protein FtsZ and application thereof. The nucleic acid sequence of the Mycoplasma bovum adhesion protein FtsZ is shown as SEQ ID NO. 1. The application also discloses a recombinant plasmid pET-30a-ftsZ and an E. coli containing the recombinant plasmid pET-30a-ftsZ. ftsZ The recombinant protein rFtsZ has the advantages of being capable of specifically combining with EBL cell membrane protein, being capable of combining with extracellular matrix components (fibronectin, fibronectin, laminin and type IV collagen), having the direct adhesion host cell effect, having good antigenicity, being capable of producing high-level antibodies, being capable of providing good immune protection effect, and providing a new target and thought for elucidating the pathogenic mechanism of the Mycoplasma bovum and developing a new vaccine and medicine.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The invention belongs to the technical field of biology, and particularly relates to a nucleus pulposus cell separation and culture method. According to the method, a step-by-step enzyme digestion method is adopted, NB6 collagenase is firstly used for primary digestion, and then compound enzyme liquid composed of trypsin and hyaluronidase is adopted for subsequent digestion; and culturing in combination with a culture vessel coated with a specific concentration of extracellular matrix component and a special culture medium. According to the method, on the premise of not depending on an immortalization technology, the primary nucleus pulposus cells with good activity can be efficiently obtained, stable amplification and long-term passage of the primary nucleus pulposus cells in vitro are realized, and specific marker expression and biological functions of the nucleus pulposus cells can be effectively maintained. The invention provides a stable cell source and technical platform for the development of cell therapy products and the research of spinal degenerative diseases.

The present invention relates to a dispersion comprising acellular dermal matrix microparticles, a filler comprising the same, and uses thereof. More specifically, the present invention relates to a filler comprising a dispersion containing acellular dermal matrix microparticles obtained by supercritical fluid extraction and high pressure dispersion operations, and a method for preparing the same. The acellular dermal filler according to the present invention has excellent biological stability and biocompatibility, and extracellular matrix components such as collagen and elastin are significantly preserved therein. In addition, the filling agent also shows excellent biochemical stability, the pH value or the collagen content and the elastin content are kept unchanged in the storage process, and the filling agent also shows physical stability and can continuously maintain injectability. Therefore, the acellular dermal filler can be effectively used as an injectable filler for repair or volume expansion of soft tissue and reduction of wrinkles in the field of plastic and regenerative medicine.

Microfluidics technology incorporating 3D gel droplets is provided for analysis of tumor cells and their interactions with immune cells, such as natural killer cells. The gel droplets include the presence of extracellular matrix components, which alter cell functions including viability and mobility. The system allows cells co-incorporated into 3D gel droplets to be observed in real-time using fluorescence microscopy, with fluorescence-based sorting of droplets for recovery of individual cells based on their function. High-quality RNA can be extracted from the sorted droplets for transcription profiling.

The invention discloses a low-energy photocuring ophthalmic sealant and a preparation method and application thereof.The ophthalmic sealant is in-situ photocuring bi-crosslinking biological hydrogel, a functional complementary composite system is constructed through collaborative optimization, and the ophthalmic sealant mainly comprises methacrylated gelatin (GelMA), a cornea extracellular matrix component, glutamine transaminase and a photoinitiator. According to the invention, a double-network construction strategy combining low-energy photocuring and enzymatic crosslinking is adopted, and curing can be triggered under the extremely low optical power density not higher than 5 mW / cm, so that the clinical application bottlenecks of tissue damage caused by too high energy of a traditional photocuring material for eyes and insufficient mechanical property and stability of a single crosslinking system are overcome. The sealing agent has excellent tissue adhesion, mechanical stability and biocompatibility in a double-crosslinking hydrogel state, can realize minimally invasive sutureless repair of corneal defects, and effectively promotes functional regeneration of corneal tissues.

The present invention relates to peptides having skin condition improving activity. The peptide of the present invention increases the expression level of an extracellular matrix component and a skin barrier enhancing factor, reduces the expression of a skin cell injury factor increased due to ultraviolet radiation, and restores the expression level of an extracellular matrix component and a skin barrier enhancing factor reduced due to ultraviolet radiation.

The application discloses a multifunctional hydrogel patch, a preparation method and application thereof, and belongs to the field of tissue engineering and regenerative medicine. The biological patch comprises a decellularized freeze-dried tumor tissue (dFCT) and a hydrogel layer loaded with hair melanin nanoparticles (HNPs). The dFCT is obtained by decellularization and freeze-drying treatment of tumor tissue, and retains abundant natural extracellular matrix components. The HNPs are extracted from hair and have excellent active oxygen scavenging capacity and photothermal conversion performance. The hydrogel layer is preferably methacrylated gelatin (GelMA). The patch shows significant cell activity promotion and antibacterial capacity in vitro, and can accelerate wound healing by reducing oxidative stress, inhibiting inflammatory response and promoting angiogenesis in vivo. The patch has the advantages of simple preparation process, natural component source, multiple functions such as antibacterial, antioxidant and tissue regeneration promotion, and is suitable for wound repair treatment.

To provide a gel composition that is transparent and has a high modulus of elasticity, containing extracellular matrix components and / or fragmented extracellular matrix components. [Solution] A gel composition containing at least one selected from the group consisting of extracellular matrix components and fragmented extracellular matrix components, and ions of a metal element.

Compositions containing an anesthetic and an extracellular matrix component or fragment thereof and methods for treating pain by topically administering such compositions are described herein.

The application discloses a preparation method of umbilical cord decellularized matrix based on perfusion decellularization technology, which comprises decellularization treatment of a complete umbilical cord by using a programmed perfusion method, and cleaning, enzymatic hydrolysis, freeze-drying and other processes. The application further discloses the umbilical cord decellularized matrix prepared by the method and application thereof. The obtained umbilical cord decellularized matrix has complete structural integrity, retains not only umbilical cord Wharton's jelly but also rich low-immunogenic extracellular matrix components in umbilical cord tissues, and has wide application prospects in the development of soft tissue injury repair, medical beauty and life beauty related products.

The invention provides a biological tissue decellularization method and a biological tissue, and relates to the technical field of biological materials. The biological tissue decellularization method provided by the invention comprises the following steps: soaking a disinfected target biological tissue in a degreasing solution to obtain a degreased target biological tissue, the degreasing solution comprising a detergent, and the degreasing solution further comprising at least one of a polyol reagent and an extracellular matrix component; and soaking the degreased target biological tissue with a pancreatin solution to obtain the decellularized target biological tissue.

The present invention relates to peptides exhibiting skin condition improving activity. The peptide of the present invention increases the expression level of an extracellular matrix component and a skin barrier enhancing factor, reduces the expression of a skin cell injury factor increased due to ultraviolet irradiation, and restores the expression level of an extracellular matrix component and a skin barrier enhancing factor reduced due to ultraviolet irradiation.

This invention discloses a method, system, and apparatus for computer-aided drug screening based on temozolomide and ADAMTS1. This application is the first to discover that ADAMTS1 is significantly enriched in recurrent gliomas following temozolomide exposure; temozolomide strongly induces a stable senescence program, leading to excessive secretion of ADAMTS1. This senescence-induced protease coordinates significant changes in extracellular matrix composition. The remodeled microenvironment acts as a mechanobiochemical signaling agent, recruiting and polarizing host-derived myeloid cells to transform into the immunosuppressive M2 phenotype, thereby conferring chemotherapy resistance to residual glioma cells through paracrine signaling. This application reveals that the "senescence-ADAMTS1-ECM-M2" axis is a key non-cellular autonomous mechanism of temozolomide resistance. Targeting ADAMTS1-mediated matrix reprogramming can eliminate the immunosuppressive niche in gliomas and overcome chemotherapy resistance, showing broad application prospects.

The present disclosure provides systems, methods, and compositions for delivering endothelial cells into Schlemm's canal to restore physiological outflow in glaucoma and other ocular disorders. The document encompasses diverse devices and hydrogel carriers suitable for delivering endothelial cells, including conduits, microcatheters, scaffolds, stents, support devices, Schlemm's canal expanders, viscodilation balloons, tensioning sutures, hydrogel sleeves, and injectable formulations. The endothelial cells may be combined with extracellular matrix components, drugs, or growth factors, and may be delivered using controlled-release hydrogels to ensure uniform circumferential seeding. The system enables removal of dysfunctional Schlemm's canal endothelial cells, OCT / OCTA-guided functional analysis, and re-endothelialization via bioengineered scaffolds. Embodiments further integrate artificial intelligence for intraoperative guidance, predictive analytics, and postoperative monitoring of endothelial integration. Safe dosing strategies are disclosed to avoid canal obstruction, and commercial applications include combination biologic-device products and digital health solutions.

Provided herein are compositions and methods for detecting migration of effector cells towards a target cell, and cytotoxicity of the migrated effector cells against the target cells. The effector cells are modified to express a homing or migratory receptor, and the target cells are modified to express the cognate ligand. The methods can be carried out in a Boyden chamber or tranwells with a porous membrane between the wells. The membrane can be coated with an extracellular matrix component to simulate a solid tumor environment.

A method for functionalizing a hollow fiber membrane for cell expansion, differentiation, harvesting, activation, or any combination thereof of target cells (e.g., natural killer cells) includes contacting a biotinylated molecule to a surface of the hollow fiber membrane including an extracellular matrix component, the biotinylated molecule binding to the extracellular matrix component and having an affinity for the target cells. The biotinylated molecule is selected from the group consisting of: cytokine, epitope, ligand, monoclonal antibody, stains, aptamer, and combinations thereof. The extracellular matrix component selected from the group consisting of: fibronectin, vitronectin, fibrinogen, collagen, laminin, streptavidin, truncations thereof, and combinations thereof.

This invention discloses a method and application for rapidly preparing humanized collagen using umbilical cord mesenchymal stem cell material, belonging to the field of biomaterial preparation technology. The key technical points of the method are as follows: S1, processing and passage-expanding umbilical cord tissue; S2, seeding the expanded umbilical cord mesenchymal stem cell suspension into a culture dish, then adding DMEM / F12 medium containing platelet lysis buffer for culture, followed by induction medium to obtain extracellular matrix; S3, enzymatically hydrolyzing the extracellular matrix, centrifuging the hydrolysate to obtain the supernatant, adjusting the pH, allowing it to stand, centrifuging again to obtain the precipitate, reconstituteing the precipitate, purifying it, centrifuging again, and then lyophilizing it; S4, forming the formulation to achieve efficient secretion and self-assembly of collagen, while utilizing extracellular matrix components (such as fibronectin and laminin) secreted by umbilical cord mesenchymal stem cells to assist collagen in forming an active product with a natural triple helix structure.

To provide a colored, fragmented extracellular matrix component capable of forming a structure with high visibility and suppressed color transfer. [Solution] A colored fragmented extracellular matrix component comprising a fragmented extracellular matrix component and a coloring agent adsorbed to the fragmented extracellular matrix component, wherein the coloring agent comprises at least one selected from the group consisting of water-insoluble and ethanol-soluble dyes and lipid-soluble dyes.

The present invention relates to peptides having skin condition improving activity. The peptide of the present invention increases the expression level of an extracellular matrix component and a skin barrier enhancing factor, again reduces the expression of a skin cell injury factor increased due to ultraviolet irradiation, and again restores the expression level of an extracellular matrix component and a skin barrier enhancing factor reduced due to ultraviolet irradiation.

The application relates to the technical field of biomaterials, in particular to a stem cell aggregate-derived decellular extracellular matrix and application thereof in bone defect repair. The decellular extracellular matrix is formed by subjecting a 3D cell aggregate in the form of a sphere to decellularization treatment, and the 3D cell aggregate is induced to form by transferring adult bone marrow mesenchymal stem cells after culture to a low-absorption cell culture carrier. The 3D dECM has a complete three-dimensional scaffold structure and abundant pores, while retaining key extracellular matrix components such as collagen, fibronectin and laminin, and is closer to the in-vivo tissue microenvironment; the 3D dECM significantly enhances the migration ability of cells, effectively improves the stemness of BMSCs, promotes extracellular matrix synthesis, and improves the mitochondrial function of BMSCs, has excellent cell regulation ability and functional activity, and thus solves the problems of low bioactivity and insufficient bionics of traditional 2D dECM.

The present invention relates to a dispersion comprising acellular dermal matrix microparticles, a membrane comprising the same, and uses thereof. More specifically, the present invention relates to a membrane comprising a dispersion containing acellular dermal matrix microparticles obtained by supercritical fluid extraction and high pressure dispersion operations, and a method for preparing the same. The acellular dermal membrane according to the present invention exhibits excellent biological stability and biocompatibility, and shows significantly better preservation of extracellular matrix components such as collagen. In addition, the material has excellent mechanical properties, so that the material is easily applied to transplantation operation and suture, and has a porous structure, so that body fluid flow is facilitated, and tissue regeneration can be supported. Thus, the acellular dermal membrane can be applied as wound dressing, adhesion barrier and implantable biomaterials such as drug delivery grafts and artificial skin in the field of regenerative medicine.

We provide protease-cleavable cytokine prodrugs. [Solution] A protease-activated procytokine is provided, comprising a cytokine polypeptide sequence; an inhibitory polypeptide sequence capable of blocking the activity of the cytokine polypeptide sequence; a linker between the cytokine polypeptide sequence and the inhibitory polypeptide sequence, comprising a protease-cleavable polypeptide sequence; and a targeting sequence designed to bind to extracellular matrix components, integrins, or syndecans, or designed to bind to extracellular matrix components, IgB (CD79b), integrins, cadherins, heparan sulfate proteoglycans, syndecans, or fibronectin with pH-sensitive functionality; or a targeting sequence comprising a variant having one or two mismatches compared to a specific sequence.

The application belongs to the technical field of biomedicine, and particularly relates to a hydrogel for evaluating functions of corneal endothelial seed cells, and a preparation method and application thereof. The hydrogel is a three-dimensional cross-linked network structure formed by free radical polymerization of acrylamide in the presence of N,N'-methylene bisacrylamide. Meanwhile, extracellular matrix components are fixed on the surface of the three-dimensional cross-linked network structure, and the hydrogel is obtained. The hydrogel prepared by the application improves the problems of mismatching of mechanical properties of traditional materials, large batch difference and the like, and provides a more reliable standardized tool for HCE cell behavior research. This breakthrough technology solves the problem that traditional materials cannot simultaneously provide physical support and function evaluation.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses a preparation method and application of a composite scaffold based on GelMA coated fat foam and bioactive glass, and the method comprises the steps of fat foam preparation, bioactive glass preparation, GelMA solution preparation, composite dipping, photo-crosslinking curing and post-treatment. A fat foam matrix and bioactive glass powder are compounded, a GelMA solution is adopted for wrapping and photo-crosslinking curing, the composite scaffold of a three-dimensional network structure is formed, the advantages of an organic matrix and an inorganic material are combined, the biocompatibility and mechanical performance of the scaffold are improved, and the scaffold is suitable for tissue engineering application; a porous fat foam matrix is prepared by decellularizing and foaming adipose tissues, natural extracellular matrix components are reserved, cell adhesion and growth are facilitated, and meanwhile, by uniformly loading bioactive glass powder, the biological activity and osteogenesis performance of the scaffold are enhanced.

The present invention relates to a peptide having an activity to improve skin conditions. The peptide of the present invention increases the expression level of an extracellular matrix component and a skin barrier function enhancing factor, reduces the expression of a skin cell injury factor increased by UV radiation, and restores the expression level of an extracellular matrix component and a skin barrier function enhancing factor reduced by UV radiation or thermal stimulation.