Method for preparing human body or animal accellular tissues

A decellularized tissue and animal tissue technology, applied in biochemical equipment and methods, tissue culture, prostheses, etc., can solve problems such as affecting product quality, tissue structure damage, long processing time, etc., to reduce procurement costs and production costs. , Short degreasing time, good product quality

Active Publication Date: 2013-07-10
上海优越生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing human or animal decellularized tissue preparation, due to the process, treatment agent, etc., takes a long time to process and has low efficiency, which can easily cause damage to the tissue structure and affect product quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing human or animal decellularized tissue, comprising the following steps:

[0023] (1) Primary tissue processing: Human or animal tissues (in this example: pleural peritoneum, esophagus mucosa, small intestine mucosa or pericardium tissue) are mechanically removed from the surface fat, and prepared into various clinically required tissues according to clinical purposes. kind of shape;

[0024] (2) Virus inactivation, degreasing and decellularization:

[0025] Proceed through the following steps in turn:

[0026] (1) Perform virus inactivation and degreasing treatment with the first inactivation and degreasing liquid for 30 minutes on the human body or animal tissue after primary processing; the first inactivation and degreasing liquid is composed of 2% peracetic acid, 1 Sodium chloride solution, 2% surfactant and the rest of water; use peracetic acid and sodium chloride solution to inactivate, and add surfactant to degrease at the same time, and ac...

Embodiment 2

[0036] A method for preparing human or animal decellularized tissue, comprising the following steps:

[0037] (1) Primary processing of tissues: removing surface fat from human or animal tissues (skin tissues) mechanically, and preparing them into various shapes required for clinical use;

[0038] (2) Virus inactivation, degreasing and decellularization:

[0039] Proceed through the following steps in turn:

[0040] (1) Use the first inactivation and degreasing solution to inactivate and degrease the virus for 10 minutes on the skin tissue of the human body or animal after initial processing; the first inactivation and degreasing solution is composed of 0.1% peracetic acid, 3% sodium chloride solution, 1% surfactant and the rest of water; use peracetic acid and sodium chloride solution to inactivate, add surfactant to degrease at the same time, and achieve virus inactivation The effect of degreasing has the characteristics of short degreasing time, high efficiency and strong...

Embodiment 3

[0050]A method for preparing human or animal decellularized tissue, comprising the following steps:

[0051] (1) Primary tissue processing: human or animal tissues (in this example, blood vessels, nerves, heart valves, bones, cartilage or tendon tissues) are mechanically removed from the surface fat, and prepared into various clinically required tissues according to clinical purposes. shape;

[0052] (2) Virus inactivation, degreasing and decellularization:

[0053] Proceed through the following steps in turn:

[0054] (1) The first inactivation and degreasing liquid is used for virus inactivation and degreasing for 20 minutes on the human body or animal tissue after primary processing; the first inactivation and degreasing liquid is composed of 1% peracetic acid, 5% sodium chloride solution, 0.5% surfactant and the rest of the water; use peracetic acid and sodium chloride solution to inactivate, add surfactant to degrease, and achieve degreasing while virus inactivation It...

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PUM

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Abstract

The invention discloses a method for preparing human body or animal accellular tissues. According to the method, the decellularized tissue obtained by treating human or animal tissues, such as skin, pleuroperitoneal membrane, esophagus mucous membrane, small intestine mucous membrane, pericardium, vessel, nervus, cardiac valves, bone, cartilage, muscle tendon, and the like through processes of inactivation of virus, derosination, decellularization, and the like is an acellular three-dimensional network structure tissue, and extracellular matrix components are kept. The tissue prepared by the method can be used as an implantable repair material for a human body and also can be used as a support material of tissue engineering cell culture. The skin, the pleuroperitoneal membrane, the esophagus mucous membrane, the small intestine mucous membrane, the pericardium, and the like also can be used as a wound covering material.

Description

technical field [0001] The invention relates to a method for preparing human or animal decellularized tissue. Background technique [0002] The existing preparation of human or animal decellularized tissue requires long processing time and low efficiency due to reasons such as process and treatment agent, which easily causes damage to the tissue structure and affects product quality. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing human or animal decellularized tissue with high production efficiency and good product quality. [0004] Technical solution of the present invention is: [0005] A method for preparing human or animal decellularized tissue, characterized in that: comprising the following steps: [0006] (1) Primary processing of tissues: remove surface fat from human or animal tissues mechanically, and prepare them into various clinically required shapes according to clinical use; [0007] (2) Virus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L15/40C12N5/00
Inventor 潘华倩
Owner 上海优越生物医学科技有限公司
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