Pet cat feed capable of promoting hair care and preparation method of pet cat feed

Abstract

The invention discloses a pet cat feed for promoting hair care and a preparation method thereof, and relates to the technical field of pet foods.The pet cat feed comprises, by mass, 90-95% of cat food base materials and 5-10% of nutrient particles; wherein the nutritional particles are prepared by taking fermented agrocybe cylindracea powder, selenomethionine, inositol and taurine as nutritional materials, adsorbing by using porous chitosan microspheres, adding gelatin and performing ultrasonic-assisted embedding. The pet cat feed provided by the invention is reasonable in formula, can meet the nutritional requirements of pet cats, can improve the appetite of the pet cats to a certain extent and promote ingestion, has positive significance in promoting growth of the pet cats, can promote hair maintenance of the pet cats and improve the hair quality of the pet cats, can reduce the peculiar smell degree during feeding of the pet cats, and is beneficial to health care of the pet cats. The physical and mental pleasure degree during pet cat feeding is improved.

Description

Technical Field

[0001] The invention relates to the technical field of pet food, and in particular to a pet cat feed promoting hair maintenance and a preparation method thereof. Background Art

[0002] Pet cat food can be roughly divided into three types: dry, canned, and semi-cooked. Dry cat food is economical and convenient, and has relatively comprehensive nutrition. It is the type of food with the highest demand among pet cats.

[0003] The basic ingredients of dry cat food include wheat, corn and other grains, as well as protein materials such as chicken and beef. In addition, nutritional supplements are added to the basic ingredients for the purpose of nutritional supplementation. At present, traditional dry cat food has poor palatability and poor attractant effect, resulting in insufficient food intake by pet cats, which is not conducive to the growth of pet cats. In addition, the protein materials it contains also have adverse effects such as strong odor in the feces of pet cats after eating.

[0004] The glossiness and beauty of a pet cat's coat will bring a better sense of pleasure to its owner. Therefore, when raising pet cats, we also pay attention to the maintenance of their hair. On the one hand, we can choose bathing products with hair maintenance functions to maintain the hair of pet cats. On the other hand, we can consider adjusting the composition of the feed and increasing nutritional supplements to improve the hair growth of pet cats.

[0005] Therefore, the present invention is proposed to promote the growth and hair maintenance of pet cats and enhance the physical and mental pleasure of raising pet cats. Summary of the invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a pet cat feed that promotes hair maintenance and a preparation method thereof.

[0007] The present invention is achieved by the following technical solution, which comprises the following steps:

[0008] The first aspect of the present invention provides a pet cat feed that promotes hair care, wherein the pet cat feed comprises, by mass percentage, 90-95% of a cat food base and 5-10% of nutrient particles; wherein the nutrient particles are made of fermented Agrocybe agrocybe powder, selenomethionine, inositol and taurine as nutrients, and are first adsorbed by porous chitosan microspheres and then added with gelatin for ultrasound-assisted embedding.

[0009] As a further optimization scheme of the present invention, the cat food base includes, by mass percentage, 30-40% corn, 5-10% rice flour, 1-5% wheat bran, 5-10% flour, 1-5% peanut shell powder, 1-4% soybean meal, 1-5% puffed soybean powder, 15-20% fish meal, 10-12% chicken liver powder, 10-12% chicken breast powder, 2-4% egg yolk powder, 2-4% chicken fat and 3-5% premix.

[0010] As a further optimization scheme of the present invention, the mass ratio of the fermented mushroom powder, thiomethionine, inositol and taurine is 1:(0.2-0.6):0.2:0.2.

[0011] As a further optimization scheme of the present invention, the fermented Agrocybe oryzae powder is first inoculated into a solid fermentation medium containing Agrocybe oryzae powder according to 10% of the total mass of the solid fermentation medium. The fermentation product is then obtained by constant temperature fermentation at 28°C for 48 hours, and finally the fermentation product is vacuum freeze-dried and ground into powder.

[0012] The second aspect of the present invention also provides a method for preparing a pet cat feed for promoting hair maintenance as described above, comprising the following steps:

[0013] (1) Fermented Agrocybe aegerita powder, selenomethionine, inositol and taurine are mixed uniformly to obtain a nutrient material, porous chitosan microspheres are prepared, the porous chitosan microspheres are dissolved in a 2% by mass acetic acid solution and stirred uniformly, the nutrient material is added, adsorbed to a saturated state at 20-30° C., then gelatin is added and stirred uniformly, ultrasonic treatment is performed, the mixture is allowed to stand and then filtered, and dried to a constant weight to obtain nutrient particles;

[0014] (2) The cat food base material is expanded and mixed with the nutrient particles obtained in step (1) to obtain the pet cat food that promotes hair care.

[0015] As a further optimization scheme of the present invention, in step (1), the amount of gelatin added is 2-3% of the total mass of the porous chitosan microspheres and the nutrient material, the ultrasonic power is 130-150 W, the ultrasonic time is 15-30 min, and the drying method is vacuum drying at a temperature of 30-45°C.

[0016] As a further optimization scheme of the present invention, in step (2), a twin-screw dry extruder is used to extrude the cat food base into oblate particles with a diameter of 5-10 mm and a degree of maturity of 85-90%.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] (1) The pet food provided by the present invention comprises a cat food base and nutrient particles. The formula is reasonable and can meet the nutritional needs of pet cats. The nutrient particles containing fermented Agrocybe oleracea powder, selenomethionine, inositol and taurine have an appetizing effect and can improve the appetite of pet cats to a certain extent and promote feeding, which has a positive significance for promoting the growth of pet cats.

[0019] (2) Before and after the feeding test, the peak breaking force and cystine content of the pet cat's hair were improved in the pet cat feed with added nutrient particles. At the same time, the glossiness and smoothness of the pet cat's hair were rated high, which can promote the maintenance of pet cat's hair and improve the quality of pet cat's hair.

[0020] (3) The pet food provided by the present invention can improve the odor of cat litter and the ammonia concentration in pet cat feces before and after the feeding test, reduce the degree of odor when raising pet cats, and improve the physical and mental pleasure of raising pet cats. DETAILED DESCRIPTION

[0021] The present invention is further described in detail below. It is necessary to point out that the following specific implementation methods are only used to further illustrate the present invention and cannot be understood as limiting the scope of protection of the present invention. Technicians in this field can make some non-essential improvements and adjustments to the present invention based on the above application content.

[0022] 1. Reagents and Materials

[0023] Unless otherwise specified, the reagents and materials used below are commercially available.

[0024] Aspergillus oryzae was Aspergillus oryzae CICC 2436 purchased from China Industrial Microbiological Culture Collection Center.

[0025] Selenomethionine was L-selenomethionine purchased from Hubei Changyao Biotechnology Co., Ltd.;

[0026] The porous chitosan microspheres were prepared in the following manner: chitosan was dissolved in an acetic acid solution with a mass percentage concentration of 2% to obtain a chitosan solution, the mass percentage concentration of the chitosan solution was 2%, the chitosan solution was gradually added dropwise to the emulsified liquid paraffin and then heated to 50°C, the volume ratio of the chitosan solution to the emulsified liquid paraffin was 1:15, and the dispersion was obtained by constant temperature stirring for 60 minutes; while stirring, an appropriate amount of a crosslinking agent formaldehyde and a calcium carbonate suspension were simultaneously added dropwise to the dispersion, and the calcium carbonate suspension and the chitosan solution were mixed. The volume ratio is 1:15, cross-linked and cured for 2 hours, an appropriate amount of isopropanol is added and stirred for 10 minutes, and the mixture is allowed to stand for stratification. The supernatant is discarded, and the precipitate is washed with isopropanol and petroleum ether for multiple times, filtered, and soaked in dilute hydrochloric acid for at least 10 minutes. After filtration, the filtrate is washed with deionized water until neutral to obtain hydrogel microspheres; the hydrogel microspheres are gradiently dehydrated in ethanol with volume percentage concentrations of 20%, 40%, 60%, 80%, and 100% for 20 minutes each, and dried in a vacuum drying oven at 35°C to constant weight.

[0027] 2. Methods

[0028] Unless otherwise specified, the methods used in the following examples are conventional methods known to those skilled in the art.

[0029] 1. Extrusion processing of cat food base

[0030] The cat food base material comprises, by mass percentage, 30-40% corn, 5-10% rice flour, 1-5% wheat bran, 5-10% flour, 1-5% peanut shell powder, 1-4% soybean meal, 1-5% puffed soybean powder, 15-20% fish meal, 10-12% chicken liver powder, 10-12% chicken breast powder, 2-4% egg yolk powder, 2-4% chicken fat and 3-5% premix. In the present embodiment, the composition of the cat food base material adopted comprises 30% corn, 5% rice flour, 2% wheat bran, 8% flour, 2% peanut shell powder, 4% soybean meal, 3% puffed soybean powder, 15% fish meal, 10% chicken liver powder, 12% chicken breast powder, 2% egg yolk powder, 3% chicken fat and 4% premix, and the premix is ​​composed of salt, calcium hydrogen phosphate, multiple trace elements and multiple vitamins.

[0031] According to the above preferred formula, the raw materials are weighed and the cat food base is expanded into oblate particles with a diameter of 5-10 mm and a degree of maturity of about 85% using a twin-screw dry extruder.

[0032] 2. Processing of nutrient particles

[0033] The ingredients of the nutrient particles include fermented Agrocybe oryzae powder, selenomethionine, inositol and taurine. The preparation method of the fermented Agrocybe oryzae powder is as follows: 10% of the total mass of the solid fermentation medium is inoculated into a solid fermentation LB medium containing Agrocybe oryzae powder. The fermentation product is obtained by constant temperature fermentation at 28°C for 48 hours. Finally, the fermentation product is vacuum freeze-dried and then ground into powder, and the number of live bacteria of the Aspergillus oryzae is not less than 5×10 8 CFU / mL.

[0034] In order to explore the influence of the component composition and mass ratio of nutrient particles on the effectiveness of pet cat feed, the component composition and mass ratio of the nutrient material were adjusted according to Table 1, and then stirred evenly to prepare nutrient material AG.

[0035] Then, the porous chitosan microspheres are dissolved in a 2% by mass acetic acid solution and stirred evenly, nutrients are added in sequence, adsorbed to saturation at 30°C, gelatin is added and stirred evenly, ultrasonically treated, filtered after standing, and dried to constant weight to obtain nutrient particles AG, wherein the amount of gelatin added is 3% of the total mass of the porous chitosan microspheres and the nutrients, the ultrasonic power is 150W, the ultrasonic time is 15min, and the drying method is vacuum drying at 30°C.

[0036] Table 1. Composition of nutritional microparticles and weight proportion of each raw material

[0037]

[0038] 3. Processing of pet cat feed

[0039] The pet cat feed comprises 90-95% of a cat food base and 5-10% of nutrient particles according to mass percentage. In this embodiment, the cat food base is preferably 90% and the nutrient particles are preferably 10%. According to the preferred mass ratio, 8 portions of the puffed cat food base are mixed evenly with the nutrient particles AF to prepare the pet cat feed AF. Meanwhile, the cat food base alone is used as the pet cat feed of the control group.

[0040] 4. Experimental Animal Grouping and Experimental Period

[0041] Thirty-two healthy 5-month-old British shorthair cats were randomly divided into 8 groups, 4 in each group, with equal gender ratios. The pre-test period was 1 week and the test period was 10 weeks. The test was conducted in a special animal house. Climbing facilities, water dishes, and food dishes were placed in the room; a litter box was placed in a special area for cat defecation. Clean and tidy once in the morning and evening every day; feed in groups, and eat and drink water freely.

[0042] 5. Detection indicators

[0043] 5.1 Growth Indicators

[0044] Growth indexes were measured by the average daily weight gain and average feed intake of each group of pet cats after the end of the trial period.

[0045] (1) Average daily weight gain: The average initial weight of each group of cats and the average final weight after the end of the trial period were recorded, and the average daily weight gain of each cat in each group was calculated.

[0046] (2) Average food intake: The daily food intake of each cat in each group from the beginning to the end of the experimental period was recorded, and the average food intake of each cat in each group was calculated.

[0047] The results are shown in Table 2.

[0048] Table 2. Effects of different pet cat diets on pet cat growth indicators

[0049]

[0050] As can be seen from Table 1, compared with feeding cat food base alone, feeding cat food AF has no adverse effects on the weight gain and food intake of cats. On the contrary, feeding cat food AF has a certain promoting effect on the weight gain and food intake of cats. Compared with cat food B, cat food E does not contain the Agrocybe aegerita powder added to the added nutritional particles that have not been fermented. From the data, it can be seen that the fermented Agrocybe aegerita powder has an appetizing effect that can improve the appetite of cats to a certain extent and promote the feeding of cats.

[0051] 5.3. Detection of coat indicators

[0052] The coat beauty and flexibility indexes include the coat brightness and softness after the test period, as well as the peak breaking force and cystine content of the pet cat's coat before and after the test period. The first two of these indicators reflect the coat beauty, and the last two reflect the coat flexibility.

[0053] Method for scoring the sensory quality of coat: the brightness of the coat is based on natural light as the light source and the brightness of the coat as the standard; the softness is judged comprehensively based on the consistency of the coat's appearance and direction and the feel; both are measured on a 10-point scale, the brighter and smoother the coat, the higher the score.

[0054] The results are shown in Table 3-4.

[0055] Table 3. Effects of different cat diets on peak force and cystine content of pet cat hair

[0056]

[0057]

[0058] As can be seen from Table 3, compared with simply feeding cat food base, feeding pet cat feed AG has a positive effect on the increase in peak force of hair pulling off and the increase in cystine content in the hair before and after the test period. Specifically, the different composition of nutrient particles in pet cat feed has different effects on the increase in peak force of hair pulling off and the increase in cystine content in the hair of pet cats. Comparing pet cat feed B with pet cat feed D, it can be seen that the effect of using selenomethionine in improving the flexibility of hair is better than methionine. After the flexibility of hair is improved, the degree of hair loss of pet cats can be reduced accordingly, which promotes the hair care of pet cats.

[0059] Table 4. Effects of different cat diets on peak force and cystine content of pet cat hair

[0060]

[0061] It can be seen from Table 4 that the results for improving the aesthetics of pet cats' coats are similar to the results for improving the flexibility of pet cats' coats in Table 3. Compared with simply feeding cat food base, feeding pet cat feed AG has a positive effect on improving the brightness and softness of pet cats' coats after the trial period. From the comparison of data in the table, it can be seen that the addition of selenomethionine in the nutritional particles has a positive effect on promoting the brightness and softness of pet cats' coats, which can promote pet cats' hair care and improve the quality of pet cats' hair.

[0062] 5.3. Detection of cat litter odor and ammonia concentration in pet cat feces

[0063] The smell of cat litter of each group of pet cats was recorded at the beginning and end of the test period. The smell of cat litter was counted by the breeder, who collected cat litter with a sampling spoon, fanned it with his hand, and counted it, which was divided into three levels: light, medium, and heavy.

[0064] In addition, the ammonia concentration in the feces of each group of pet cats at the beginning and end of the test period was recorded. When measuring, the pet cat feces sample was diluted 10 times, and then a 10 mol / L NaOH solution (ionic strength regulator) was added. The volume ratio of NaOH solution to feces sample was 1:100, so that the pH value of the feces sample solution increased to about 11, and the ammonium ions were completely converted into ammonia, which diffused through the hydrophobic polymer breathable film into the extremely thin liquid layer between the breathable membrane and the sensitive membrane of the ion selective electrode until the ammonia partial pressure in the test solution and this extremely thin liquid layer was equal. The ammonia pressure was obtained by measuring the solution potential, and then the ammonia concentration was obtained.

[0065] The results are shown in Table 5.

[0066] Table 5. Effects of different cat diets on cat litter odor and ammonia concentration in cat feces

[0067]

[0068] As can be seen from Table 5, simply feeding cat food base has no obvious improvement effect on the smell of cat litter and the ammonia concentration in cat feces before and after the test. After adding nutrient particles to the cat food base, at the end of the test period, the smell of cat litter and the ammonia concentration in cat feces decreased compared with the beginning of the test period. From the data in the table, it can be further seen that the composition of nutrient particles has an effect on improving the smell of cat litter and the ammonia concentration in cat feces. Specifically, by comparing pet cat feed B with pet cat feed E and pet cat feed G, it can be seen that compared with the commonly used alfalfa powder, the use of mushroom powder will improve the smell of cat litter and reduce the ammonia concentration in cat feces accordingly. Further using fermented Agrocybe agrocybe powder instead of unfermented Agrocybe agrocybe powder has a better improvement effect.

[0069] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A pet cat food for promoting hair care, characterized in that: The pet cat feed comprises 90-95% of a cat food base and 5-10% of nutrient particles in terms of mass percentage; wherein the nutrient particles are made of fermented Agrocybe agrocybe powder, selenomethionine, inositol and taurine as nutrients, and are first adsorbed by porous chitosan microspheres and then added with gelatin for ultrasound-assisted embedding.

2. A pet cat food for promoting hair care according to claim 1, characterized in that: The cat food base material comprises, by mass percentage, 30-40% corn, 5-10% rice flour, 1-5% wheat bran, 5-10% flour, 1-5% peanut shell powder, 1-4% soybean meal, 1-5% puffed soybean powder, 15-20% fish meal, 10-12% chicken liver powder, 10-12% chicken breast powder, 2-4% egg yolk powder, 2-4% chicken fat and 3-5% premix.

3. A pet cat food for promoting hair care according to claim 1, characterized in that: The mass ratio of the fermented mushroom powder, thiomethionine, inositol and taurine is 1:(0.2-0.6):0.2:0.

2.

4. The pet cat food for promoting hair maintenance according to claim 1, characterized in that: The fermented Agrocybe agrocybe powder is obtained by first inoculating Aspergillus oryzae into a solid fermentation medium containing Agrocybe agrocybe powder according to 10% of the total mass of the solid fermentation medium, then subjecting the fermentation product to constant temperature fermentation at 28°C for 48 hours, and finally subjecting the fermentation product to vacuum freeze-drying and grinding into powder.

5. A method for preparing a pet cat feed for promoting hair care as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Fermented Agrocybe aegerita powder, selenomethionine, inositol and taurine are mixed uniformly to obtain a nutrient material, porous chitosan microspheres are prepared, the porous chitosan microspheres are dissolved in a 2% by mass acetic acid solution and stirred uniformly, the nutrient material is added, adsorbed to a saturated state at 20-30° C., then gelatin is added and stirred uniformly, ultrasonic treatment is performed, the mixture is allowed to stand and then filtered, and dried to a constant weight to obtain nutrient particles; (2) The cat food base material is expanded and mixed evenly with the nutrient particles obtained in step (1) to obtain the pet cat food for promoting hair care.

6. The method for preparing a pet cat feed for promoting hair maintenance according to claim 5, characterized in that: In step (1), the amount of gelatin added is 2-3% of the total mass of the porous chitosan microspheres and the nutrient material, the ultrasonic power is 130-150 W, the ultrasonic time is 15-30 min, and the drying method is vacuum drying at a temperature of 30-45°C.

7. The method for preparing a pet cat feed for promoting hair maintenance according to claim 5, characterized in that: In step (2), a twin-screw dry extruder is used to extrude the cat food base into oblate particles with a diameter of 5-10 mm and a degree of maturity of 85-90%.

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