115 results about "Virus inactivation" patented technology

The invention relates to the technical field of repair materials, and particularly discloses an extracellular matrix fiber material as well as a preparation method and application thereof. The invention discloses a preparation method of an extracellular matrix fiber material. The preparation method comprises the following steps: taking animal tissues, and carrying out virus inactivation, decellularization and antigen removal, degreasing, drying, grinding and sterilization; the decellularization and antigen removal method comprises the following specific steps: sequentially soaking a sample with a surfactant solution for h; then, soaking treatment is carried out by adopting a salt-alkali solution which contains 0.05-3 mol / L of salt and has the pH value of 8-11; then carrying out soaking treatment by adopting a 0.1-1wt% enzyme solution; and in degreasing, one or more of normal hexane, trichloromethane, dichloromethane, methanol, ethanol, acetone, diethyl ether and ethyl acetate are used for soaking the sample. According to the technical scheme, the ECM powder particle material with high growth factor content is prepared.

The invention relates to the field of ultraviolet sterilization systems, in particular to an integrated ultraviolet sterilization system utilizing a UVC deep ultraviolet chip, which comprises a UVC deep ultraviolet chip lamp bead module serving as a core light source to emit deep ultraviolet light for sterilization; the self-adaptive heat dissipation module is attached to the UVC deep ultraviolet chip lamp bead module through heat conduction silicone grease and used for dynamically guiding out heat of the UVC deep ultraviolet chip lamp bead module; and the optical power closed-loop driving module is electrically connected with the UVC deep ultraviolet chip lamp bead module and is used for outputting stable current and performing feedback regulation according to optical power. According to the integrated ultraviolet sterilization system using the UVC deep ultraviolet chip, the high efficiency is remarkably improved: the NPSS + superlattice insertion layer enables the electro-optical conversion efficiency to be improved to more than 8%, and the deep ultraviolet light of 265-275 nm is 1t; the inactivation rate of escherichia coli and influenza viruses within 1 minute reaches 99.99%, and the disinfection and killing efficiency is improved by 30% compared with that of existing equipment.

The invention relates to a method for inactivating viruses in a fluid, in which a starting fluid (AF) laden with an active virus is mixed in a first mixing device (2) with a virus inactivation reagent (R1) to form a reactive fluid (F) and the active virus is inactivated by incubating the reactive fluid (F), and stopping the inactivation of the virus in the reactive fluid (F) by mixing the reactive fluid (F) with another reagent (R2) or by removing the virus inactivation reagent (R1) from the reactive fluid (F), thereby producing the resulting fluid (RF). It is proposed that the active virus is inactivated by incubating a reactive fluid (F) in a container device (3) different from the first mixing device (2) and comprising a plurality of separate containers (4), the reactive fluid (F) being guided from the first mixing device (2) to the container device (3) before incubation.

The application discloses a kind of enveloped virus inactivation preparation based on medium-chain triglyceride and application thereof, the present application relates to the technical field of biological medicine external preparation, including active ingredient and auxiliary material: the active ingredient includes: high-purity medium-chain triglyceride (MCT), preferably octanoic acid (C8) and capric acid (C10) triglyceride mixture, purity ≥99%; The auxiliary material includes: food-grade antioxidant, for preventing oxidation of preparation, the content of food-grade antioxidant is 0.01-0.05%w / w, with medium-chain triglyceride as active ingredient, through the physical and chemical synergistic effect, enveloped virus is efficiently killed, for treating stubborn skin mucosa infection diseases (such as stubborn flat warts, herpes labialis, genital herpes, etc.) caused by human papillomavirus (HPV), herpes simplex virus (HSV-1 / HSV-2), varicella-zoster virus and other enveloped viruses.

Embodiments of the present disclosure describe a system and method for controlling the pH of a polypeptide-containing mixture. The pH may be controlled for any suitable purpose, for example, to inactivate a virus in the mixture. The method may include the step of eluting a mixture (e.g., an eluent) having a pH greater than, for example, 3.9 and, for example, less than, 8.5 from a chromatography column. The method may further include one or more of the steps of measuring the protein concentration of the mixture and measuring the pH of the mixture. Subsequently, the amount of acid required to reduce the pH of the mixture to a target pH may be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After calculating the amount of acid to be added, a portion of the acid may be added to the mixture, wherein said portion of the acid is sufficient to achieve the target pH.

The application relates to a double-metal hydroxide, in particular to application of a CuAl double-metal hydroxide, in particular to application of the CuAl double-metal hydroxide in preparation of a preparation for inactivation of pathogenic bacteria, fungi and viruses. The CuAl double-metal hydroxide is prepared by a formamide liquid phase stripping method. Compared with the prior art, the application solves the problem of high cost of a silver ion loaded layered double-metal hydroxide in the prior art, and realizes preparation of a low-cost, high-performance and green antibacterial material with a bactericidal and antiviral effect.

The present invention relates to the field of biomedicine, and more particularly to a method for purifying an inactivated rabies virus vaccine. The method comprises the following steps: preparing an inactivated rabies virus ultrafiltration concentrate: purifying the inactivated rabies virus ultrafiltration concentrate by chromatography; the chromatography purification specifically comprises the following steps: column cleaning: loading a composite filler into a chromatography column, connecting an AKTA to the chromatography column, cleaning, sterilizing, and setting aside; chromatography operation: equilibrating the chromatography column with PBS buffer; sample loading: loading the inactivated rabies virus ultrafiltration concentrate into the chromatography column, eluting with an eluent, and collecting the eluate corresponding to the absorption peak at an ultraviolet wavelength of 280 nm, which is the purified inactivated rabies virus vaccine; column cleaning; and results showing that the residual protein content is as low as 5.0 ng / ml, the antigen content can reach above 16.0 IU / ml, the host protein removal rate can reach above 99%, and the antigen content recovery rate can reach above 86%.

The application discloses a preparation method of a tetravalent influenza virus split vaccine and belongs to the technical field of vaccines. The preparation method of the tetravalent influenza virus split vaccine comprises the following steps: pre-incubation of a chicken embryo, pre-embryo inspection, virus inoculation and culture, post-embryo inspection, cold embryo, virus harvesting, ultrafiltration clarification, zone centrifugation, desugaring, chromatography, virus splitting, virus inactivation, ultrafiltration liquid replacement, sterilization filtration and preparation of a tetravalent influenza virus split vaccine product. Compared with the prior art, the technical means adopted in the application effectively reduces the content of free formaldehyde and improves the antibody level of the vaccine.

The utility model discloses a frost prevention and dust removal device and heat exchange system based on plasma wind, including a plurality of plasma wind generation modules, the plurality of plasma wind generation modules are arranged in array, each plasma wind generation module includes an ionization unit and a high voltage conversion unit, the high voltage conversion unit is connected with a power supply, and the ionization unit is connected with the power supply. The high-voltage conversion unit is used for converting power supply voltage into high-voltage electricity needed by ionization, the ionization unit is connected with the high-voltage conversion unit, the high-voltage electricity generated by the high-voltage conversion unit is applied to the ionization unit to form a high-voltage electric field, gas molecules in air are ionized to form plasma wind, water molecule frosting can be restrained, a formed frost layer is decomposed, and the air quality is improved. Reverse circulation of the heat exchange system is not needed, the heating or refrigerating stability is improved, the service life of a compressor, a valve and other key components is prolonged, in addition, plasma air can further conduct dust removal, organic pollutant decomposition, sterilization and virus inactivation on the surface of the evaporator, the heat exchange efficiency can be improved, the operation and maintenance cost can be reduced, and the application range of the heat exchange system can be expanded.

The invention provides a method for predicting the ultraviolet inactivation effect of viruses in a liquid biological product. The method comprises the following steps: (1) detecting the absorbance value of a to-be-detected liquid biological product at a preset wavelength; (2) constructing a relation curve of the ultraviolet dose of the BSA / virus mixed solution and the decline degree of the virus titer; (3) constructing a relation curve of the ultraviolet dose of the SMZ working solution and the SMZ degradation degree; (4) constructing a relation curve of the operation parameters and the SMZ degradability; and (5) substituting preset operation parameters into the relation curve of the operation parameters and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the decline of the indication virus titer, and judging the ultraviolet inactivation effect of the virus according to a predicted value of the decline of the indication virus titer. The method is accurate in prediction result, does not need to consume liquid biological products, and can be used for various virus inactivation effects under the condition that the ultraviolet dose cannot be accurately calculated.

The invention relates to an application of a cerium oxide nano-material in a vaccine adjuvant and / or a vaccine inactivator, in particular to a vaccine, when the cerium oxide nano-material is the vaccine adjuvant, the vaccine comprises the vaccine adjuvant and an immunogen; or when the cerium oxide nano material is a vaccine inactivating agent, the vaccine is a composite vaccine, the composite vaccine comprises the vaccine inactivating agent and a virus, and the virus is embedded in the cerium oxide nano material; the preparation method of the composite vaccine comprises the step of mineralizing and inactivating viruses by using a vaccine inactivator, wherein the vaccine inactivator is cerium salt. The cerium oxide can be used as a vaccine adjuvant and also can be used as a virus inactivator. The maturation of dendritic cells and macrophages can be promoted by means of regulating the level of active oxygen in immune cells and the like, and the antigen presentation efficiency is improved, so that the vaccine-induced body fluid and cellular immune response is enhanced; meanwhile, cerium salt can directly act on virus surface protein for mineralization, efficient and safe virus inactivation is achieved, and preparation of inactivated vaccines is facilitated.

The invention provides a biological product virus inactivation device and application thereof.The biological product virus inactivation device comprises an outer cylinder support, an outer cylinder, an inner cylinder, a collecting bin, an ultraviolet lamp and a driving device, the outer cylinder is arranged on the outer cylinder support and is of a hollow structure with the two ends open, and the axis of the outer cylinder is obliquely arranged relative to the horizontal plane; the inner cylinder is rotatably arranged in the outer cylinder around the axis of the inner cylinder, the inner cylinder is of a hollow structure with two open ends, and the axis of the inner cylinder coincides with the axis of the outer cylinder; the opening of the collecting bin is positioned below the lower end opening of the inner cylinder; the ultraviolet lamp is a strip-shaped lamp and is fixedly arranged in the inner cylinder or between the outer wall of the inner cylinder and the inner wall of the outer cylinder; the driving device is arranged between the outer cylinder and the inner cylinder and can drive the inner cylinder to rotate around the axis of the inner cylinder. The biological product virus inactivation device is more convenient to operate, biological product feed liquid is not prone to bubbling in the virus inactivation process, the activity of a biological product can be better reserved, and the biological product virus inactivation device is suitable for large-scale industrial treatment.

A virus inactivation method is provided. The method includes irradiating a solution containing an artificially expressed antibody or antibody fragment with ultraviolet rays in the presence of a radical scavenger. The irradiating only aggregates 5% or less of the antibody or antibody fragment. A wavelength of the ultraviolet rays is 200 nm or longer and 315 nm or shorter. An irradiation amount of the ultraviolet rays is 300 mJ / cm2 or more. An irradiation time of the ultraviolet rays is 3 minutes or less. A concentration of the radical scavenger in the solution is 0.03 mM or more.

This invention discloses an emergency vehicle-mounted isolation face shield, belonging to the field of personal protective equipment technology. The face shield includes a main body, an airtight fit structure, a dual-path independent airflow module, a multi-stage virus filtration and purification unit, an external air supply interface, an anti-fog window, and quick-fix components. The main body is integrally formed from a transparent anti-fog window and flexible edging; the airtight fit structure uses a three-section silicone sealing gasket combined with a micro-groove air-guiding structure to achieve a 360° low-leakage seal; the dual-path independent airflow module achieves physical separation of inhaled and exhaled air; the multi-stage filtration unit includes a pre-filter layer, a high-efficiency nano-filter layer, a virus inactivation layer, and a deodorizing adsorption layer; the external air supply interface can be connected to a vehicle-mounted air supply system, achieving unlimited endurance and automatic air source switching. This invention solves the problems of poor airtightness, low filtration efficiency, breathing difficulties, limited endurance, and inconvenience in emergency situations found in existing face shields, and is suitable for various scenarios such as infectious disease control, fire escape, and vehicle-mounted emergency response.

To provide a virus inactivation composition having a high inactivating effect against non-enveloped viruses.SOLUTION: A composition for inactivating non-enveloped viruses, comprising (a) monoethanolamine, (b) an aromatic alcohol, and water, and having a pH of 10.5 or more and 14 or less at 25°C.SELECTED DRAWING: None

The present invention relates to methods of viral inactivation with a mixture of solvent and detergent. In particular, the present invention is directed to a method for preparing a virus-inactivated polypeptide solution wherein a polypeptide material is first loaded into an affinity chromatography column then incubated with said mixture of a solvent and a detergent within the affinity chromatography column.

The application relates to a method for inactivating viruses in sewage. The method comprises the following steps: introducing heterotrophic bacteria into sewage to be inactivated; increasing the carbon-nitrogen ratio of the sewage during the activation and expansion of the heterotrophic bacteria; washing and resuspending the activated and expanded heterotrophic bacteria to form a heterotrophic bacteria liquid to be used; inoculating the heterotrophic bacteria liquid into the sewage and reducing the carbon-nitrogen ratio of the sewage, so that the heterotrophic bacteria produce an oxidative stress response to inactivate viruses. The method adjusts the carbon-nitrogen ratio to make the heterotrophic bacteria change from an eutrophic environment to an oligotrophic environment, activates the oxidative stress pathway of the heterotrophic bacteria, increases the generation of oxidative active species such as superoxide free radicals, causes oxidative damage to the virus shell or envelope, and thus promotes the inactivation of viruses. The method is simple and efficient, and has a wide application scenario.

The invention discloses an HPV virus inactivation dressing and a preparation method thereof, and relates to the field of HPV virus inactivation dressings, the HPV virus inactivation dressing comprises, by weight, 50-90 parts of animal extract, 5-15 parts of morchella, 5-10 parts of sodium carboxymethyl cellulose, 5-10 parts of gelatin and 30-35 parts of purified water. Through the synergistic effect of the animal extract, the morchella esculenta and special substances, HPV viruses can be efficiently inactivated, and the detergent has sterilization and anti-inflammation effects and is suitable for various detergent products; through the synergistic effect of the animal extract and the morchella esculenta, the HPV virus can be efficiently inactivated, and experimental results show that the inactivation rates of HPV16 type pseudoviruses and HPV18 type pseudoviruses reach 90.53% and 98.79% respectively.

The invention relates to the technical field of furniture design, in particular to a bed with a negative oxygen ion releasing function, which comprises a bed body, a mattress, a negative ion bed sheet and a negative ion latex mattress, the mattress is fixedly mounted at the top of the bed body, the negative ion bed sheet is laid at the top of the mattress, and the negative ion latex mattress is embedded in the mattress; pine needle extraction spray is evenly sprayed on the surface of cloth of the anion bed sheet, and the core component of the pine needle extraction spray is natural pine needle extract. According to the pine needle extraction spray, the plant ecological level negative oxygen ion technology is adopted, negative oxygen ions are passively and continuously released, the concentration of the negative oxygen ions in the sleep environment is stabilized to be 1000 pieces / cm or above, the health threshold value for improving immunity is achieved, the released negative oxygen ions can adsorb and settle particulate matters such as PM2.5 and dust, meanwhile, external structures of bacteria and viruses are destroyed, and the effect of improving the sleep quality is achieved. The sterilization rate on escherichia coli, staphylococcus aureus and the like is more than 99%, and the inactivation rate on new coronavirus reaches 99.96%. The health preserving effect is realized.

Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange / hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration / diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.

The utility model belongs to virus inactivation technical field discloses a kind of continuous online virus inactivation, incubation system, including sample stirrer, first delivery pump, first inactivator storage tank, second delivery pump, mixed stirrer, first four-way valve, concentration module, third delivery pump, first online detection module, second four-way valve, incubation module, second online detection module and finished product collection tank.Sample in sample stirrer and inactivator in first inactivator storage tank are continuously delivered to mixed stirrer according to required proportion, after mixing, it can be selected to enter concentration module concentration and enter first online detection module or directly enter first online detection module and carry out detection, the solution after detection can be selected to enter incubation module, after incubation, enter second online detection module or directly enter second online detection module and carry out detection, after detection, enter finished product collection tank and collect.The continuous online virus inactivation, incubation system can realize the automation of virus inactivation and incubation.

The inventions provide improved systems and methods for inline concentrating and reducing intermediate process pools to mitigate plant fit challenges associated with high-titer protein purification process. The inventions also provide methods for concentrating and reducing VI pool volumes by integrating columns of single pass tangential flow filtration (SPTFF) system with viral inactivation pool filtration (VIPF) in protein purification process. Therapeutic proteins purified by the methods also are provided.

The invention belongs to the technical field of livestock and poultry feed transportation, and particularly relates to a livestock feed transportation device which comprises a carrying body and a discharging assembly arranged on the carrying body and further comprises a dynamic ventilation mechanism and a pretreatment mechanism, the dynamic ventilation mechanism is arranged on the carrying body, and the pretreatment mechanism is arranged on the carrying body; the pretreatment mechanism comprises an atomization disinfection assembly and a centrifugal dispersion assembly, the atomization disinfection assembly is arranged on the pretreatment mechanism, and the centrifugal dispersion assembly is arranged on the pretreatment mechanism; hydrogen peroxide is atomized through the pretreatment mechanism and the ultrasonic atomization sheet to generate micron-sized particles, centrifugal dispersion of the rotating disc and rolling of the first spiral blade and the second spiral blade are combined, it is ensured that the particles permeate gaps and surfaces of feed, bacterium and virus inactivation is improved, and the problem that a blind area is difficult to cover through traditional spraying is solved.

The invention is suitable for the technical field of medical equipment, and provides a virus inactivation device and an inactivation method. The virus inactivation device comprises: a device body; the rotating mechanism comprises a rotating disc and a driving mechanism for driving the rotating disc to rotate; the rotating disc is rotationally mounted at the bottom of the device body; a plurality of storing and carrying discs are arranged on the top of the rotating disc in an array mode, and a rotation mechanism is further arranged between the storing and carrying discs and the device body and used for driving the storing and carrying discs to rotate; the fixing mechanism is arranged on the storing and carrying disc and comprises a first vertical net plate, a second vertical net plate and a horizontal support, the first vertical net plate and the second vertical net plate are arranged in parallel, the horizontal support is fixedly installed at the bottom of the second vertical net plate, and a storing and carrying cavity is defined by the first vertical net plate, the second vertical net plate and the horizontal support; the storage cavity is used for placing a blood bag; and the sterilizing lamp is arranged on the inner wall of the device main body and is used for carrying out virus inactivation treatment on the blood bag on the fixing mechanism.

The utility model relates to the field of vegetable planting, and discloses a microbial agent large-area spraying device for passivating viruses in vegetable planting, which comprises a base, a stirring box is fixedly mounted at the top end of the base, and a first motor is fixedly mounted at the top end of the stirring box; the output end of the first motor rotationally penetrates through the top end of the stirring box and is fixedly connected with the top end of the rotating shaft through a coupler, a plurality of stirring rods are fixedly connected to the two sides of the rotating shaft, and a mounting frame is fixedly mounted at the position, located on one side of the stirring box, of the top end of the base; the output end of the second motor rotationally penetrates through the center of the top end of the mounting frame and is fixedly connected with the bottom end of a rotating disc, an adjusting assembly is fixedly connected to the top end of the rotating disc, a mounting plate is fixedly connected to the top end of the adjusting assembly, and a spraying pipe is fixedly mounted at the top end of the mounting plate through a plurality of hoops; and a plurality of spray heads are fixedly mounted at the bottom end of one side of the spraying pipe. According to the device, pesticide spraying in the vegetable planting process is facilitated.

The invention provides a virus inactivation method of a liquid biological product, which adopts a virus inactivation device to carry out virus inactivation on the liquid biological product, and the virus inactivation device comprises a box body, a console, an overflowing container arranged in the box body and an ultraviolet lamp group arranged in the box body, a liquid biological product is continuously introduced into the liquid inlet buffer bin through the peristaltic pump, upwards overflows through the liquid inlet buffer bin, enters the temporary liquid storage bin after passing through the plurality of quartz round tubes, and is finally discharged from the second flow guide silicone tube; the liquid biological product is irradiated by the first ultraviolet lamp and the second ultraviolet lamp to inactivate viruses in the process of flowing in the quartz round tubes. According to the invention, the virus inactivation effect can be improved while the biological activity of the liquid biological product is ensured, and continuous and stable virus inactivation of the liquid biological product can be realized. The virus inactivation device is easy to clean and maintain and long in service life, and the virus inactivation method based on the virus inactivation device is more economical, simpler and more convenient and suitable for large-scale treatment of liquid biological products.

The application discloses a thrombin solution virus inactivation device, which comprises a pump body one and a rack, the input end of the pump body one is communicated with a liquid storage device for storing thrombin solution through a liquid inlet pipe, the output end of the pump body one is provided with a liquid outlet pipe one, a protective shell is arranged at the middle position of the rack, a light transmission cylinder for placing the thrombin solution is arranged in the protective shell, a plurality of pairs of semicircular protrusions for stirring the liquid are arranged on the inner wall of the light transmission cylinder alternately, a secondary transmission gear is fixedly arranged at one end of the light transmission cylinder, the liquid outlet pipe one penetrates through the secondary transmission gear and extends into the light transmission cylinder, a plurality of ultraviolet lamps are arranged in the annular distribution between the light transmission cylinder and the protective shell, a driving motor is arranged at the top of the rack, a primary transmission gear corresponding to the secondary transmission gear is fixedly connected to the output end of the driving motor, and the primary transmission gear and the secondary transmission gear are transmissionally connected through a transmission belt, the semicircular protrusions arranged on the inner wall of the light transmission cylinder alternately can generate a turbulence effect on the thrombin solution in the light transmission cylinder when the light transmission cylinder rotates, the solution is promoted to be stirred more uniformly, the ultraviolet light can be refracted, the optical path length is increased, the ultraviolet light is more fully contacted with the solution, and thus the virus inactivation efficiency is improved.

The invention relates to a method for separating and purifying fibrinogen with low impure protein, and belongs to the technical field of blood product production. The method comprises the following steps: preparing a raw material component I, dissolving and filtering an FI precipitate, dissolving with a dissolving solution A, and filtering to obtain a filtrate A; purifying and separating polyethylene glycol, adjusting the pH value of the filtrate A, adding polyethylene glycol to precipitate, dissolving the precipitate by using the dissolving solution B, and filtering; s / D inactivation: adding an S / D solution into the filtrate B for virus inactivation; ethanol purification and separation: adding ethanol to the inactivated solution to precipitate protein, dissolving the precipitate with a dissolving solution B, and filtering; purifying and separating glycine, adding glycine precipitation protein into the filtrate C, dissolving the precipitate by using the dissolving solution C, and filtering; carrying out ultrafiltration dialysis, and dialyzing and concentrating the filtrate D; preparation: diluting the stock solution to a proper concentration; the semi-finished product is subpackaged, freeze-dried and subjected to dry heat inactivation treatment. The method can effectively remove impure proteins, improve the purity of fibrinogen and ensure the product safety.

An UV-C device may include several UV-C light sources (e.g., UV-C LEDs) and such UV-C LEDs may have UV-C reflecting structures arranged to direct UV-C in a particular direction and at a particular size and shape. Doing so may, for example, increase the UV-C in a particular direction or working area. A UV-C generating device may be utilized in an air stream, such as an air duct, to sterilize air from that air stream. Sound suppression compartments may be placed around a UV-C generating device inlet and / or a device outlet to reduce sound from the UV-C generating device. Human perceivable (e.g., audible, tactile, and / or visual) notifications may be utilized to provide notification of different modes of operation and / or different efficacy levels (e.g., percent ranges of inactivation of a particular or multiple particular viruses, bacteria, spores, etc.