74 results about "Virus inactivation" patented technology

The invention relates to the technical field of repair materials, and particularly discloses an extracellular matrix fiber material as well as a preparation method and application thereof. The invention discloses a preparation method of an extracellular matrix fiber material. The preparation method comprises the following steps: taking animal tissues, and carrying out virus inactivation, decellularization and antigen removal, degreasing, drying, grinding and sterilization; the decellularization and antigen removal method comprises the following specific steps: sequentially soaking a sample with a surfactant solution for h; then, soaking treatment is carried out by adopting a salt-alkali solution which contains 0.05-3 mol / L of salt and has the pH value of 8-11; then carrying out soaking treatment by adopting a 0.1-1wt% enzyme solution; and in degreasing, one or more of normal hexane, trichloromethane, dichloromethane, methanol, ethanol, acetone, diethyl ether and ethyl acetate are used for soaking the sample. According to the technical scheme, the ECM powder particle material with high growth factor content is prepared.

The application discloses a kind of enveloped virus inactivation preparation based on medium-chain triglyceride and application thereof, the present application relates to the technical field of biological medicine external preparation, including active ingredient and auxiliary material: the active ingredient includes: high-purity medium-chain triglyceride (MCT), preferably octanoic acid (C8) and capric acid (C10) triglyceride mixture, purity ≥99%; The auxiliary material includes: food-grade antioxidant, for preventing oxidation of preparation, the content of food-grade antioxidant is 0.01-0.05%w / w, with medium-chain triglyceride as active ingredient, through the physical and chemical synergistic effect, enveloped virus is efficiently killed, for treating stubborn skin mucosa infection diseases (such as stubborn flat warts, herpes labialis, genital herpes, etc.) caused by human papillomavirus (HPV), herpes simplex virus (HSV-1 / HSV-2), varicella-zoster virus and other enveloped viruses.

Embodiments of the present disclosure describe a system and method for controlling the pH of a polypeptide-containing mixture. The pH may be controlled for any suitable purpose, for example, to inactivate a virus in the mixture. The method may include the step of eluting a mixture (e.g., an eluent) having a pH greater than, for example, 3.9 and, for example, less than, 8.5 from a chromatography column. The method may further include one or more of the steps of measuring the protein concentration of the mixture and measuring the pH of the mixture. Subsequently, the amount of acid required to reduce the pH of the mixture to a target pH may be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After calculating the amount of acid to be added, a portion of the acid may be added to the mixture, wherein said portion of the acid is sufficient to achieve the target pH.

The application relates to a double-metal hydroxide, in particular to application of a CuAl double-metal hydroxide, in particular to application of the CuAl double-metal hydroxide in preparation of a preparation for inactivation of pathogenic bacteria, fungi and viruses. The CuAl double-metal hydroxide is prepared by a formamide liquid phase stripping method. Compared with the prior art, the application solves the problem of high cost of a silver ion loaded layered double-metal hydroxide in the prior art, and realizes preparation of a low-cost, high-performance and green antibacterial material with a bactericidal and antiviral effect.

The application discloses a preparation method of a tetravalent influenza virus split vaccine and belongs to the technical field of vaccines. The preparation method of the tetravalent influenza virus split vaccine comprises the following steps: pre-incubation of a chicken embryo, pre-embryo inspection, virus inoculation and culture, post-embryo inspection, cold embryo, virus harvesting, ultrafiltration clarification, zone centrifugation, desugaring, chromatography, virus splitting, virus inactivation, ultrafiltration liquid replacement, sterilization filtration and preparation of a tetravalent influenza virus split vaccine product. Compared with the prior art, the technical means adopted in the application effectively reduces the content of free formaldehyde and improves the antibody level of the vaccine.

The utility model discloses a frost prevention and dust removal device and heat exchange system based on plasma wind, including a plurality of plasma wind generation modules, the plurality of plasma wind generation modules are arranged in array, each plasma wind generation module includes an ionization unit and a high voltage conversion unit, the high voltage conversion unit is connected with a power supply, and the ionization unit is connected with the power supply. The high-voltage conversion unit is used for converting power supply voltage into high-voltage electricity needed by ionization, the ionization unit is connected with the high-voltage conversion unit, the high-voltage electricity generated by the high-voltage conversion unit is applied to the ionization unit to form a high-voltage electric field, gas molecules in air are ionized to form plasma wind, water molecule frosting can be restrained, a formed frost layer is decomposed, and the air quality is improved. Reverse circulation of the heat exchange system is not needed, the heating or refrigerating stability is improved, the service life of a compressor, a valve and other key components is prolonged, in addition, plasma air can further conduct dust removal, organic pollutant decomposition, sterilization and virus inactivation on the surface of the evaporator, the heat exchange efficiency can be improved, the operation and maintenance cost can be reduced, and the application range of the heat exchange system can be expanded.

The invention provides a method for predicting the ultraviolet inactivation effect of viruses in a liquid biological product. The method comprises the following steps: (1) detecting the absorbance value of a to-be-detected liquid biological product at a preset wavelength; (2) constructing a relation curve of the ultraviolet dose of the BSA / virus mixed solution and the decline degree of the virus titer; (3) constructing a relation curve of the ultraviolet dose of the SMZ working solution and the SMZ degradation degree; (4) constructing a relation curve of the operation parameters and the SMZ degradability; and (5) substituting preset operation parameters into the relation curve of the operation parameters and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the decline of the indication virus titer, and judging the ultraviolet inactivation effect of the virus according to a predicted value of the decline of the indication virus titer. The method is accurate in prediction result, does not need to consume liquid biological products, and can be used for various virus inactivation effects under the condition that the ultraviolet dose cannot be accurately calculated.

The invention relates to an application of a cerium oxide nano-material in a vaccine adjuvant and / or a vaccine inactivator, in particular to a vaccine, when the cerium oxide nano-material is the vaccine adjuvant, the vaccine comprises the vaccine adjuvant and an immunogen; or when the cerium oxide nano material is a vaccine inactivating agent, the vaccine is a composite vaccine, the composite vaccine comprises the vaccine inactivating agent and a virus, and the virus is embedded in the cerium oxide nano material; the preparation method of the composite vaccine comprises the step of mineralizing and inactivating viruses by using a vaccine inactivator, wherein the vaccine inactivator is cerium salt. The cerium oxide can be used as a vaccine adjuvant and also can be used as a virus inactivator. The maturation of dendritic cells and macrophages can be promoted by means of regulating the level of active oxygen in immune cells and the like, and the antigen presentation efficiency is improved, so that the vaccine-induced body fluid and cellular immune response is enhanced; meanwhile, cerium salt can directly act on virus surface protein for mineralization, efficient and safe virus inactivation is achieved, and preparation of inactivated vaccines is facilitated.

A virus inactivation method is provided. The method includes irradiating a solution containing an artificially expressed antibody or antibody fragment with ultraviolet rays in the presence of a radical scavenger. The irradiating only aggregates 5% or less of the antibody or antibody fragment. A wavelength of the ultraviolet rays is 200 nm or longer and 315 nm or shorter. An irradiation amount of the ultraviolet rays is 300 mJ / cm2 or more. An irradiation time of the ultraviolet rays is 3 minutes or less. A concentration of the radical scavenger in the solution is 0.03 mM or more.

This invention discloses an emergency vehicle-mounted isolation face shield, belonging to the field of personal protective equipment technology. The face shield includes a main body, an airtight fit structure, a dual-path independent airflow module, a multi-stage virus filtration and purification unit, an external air supply interface, an anti-fog window, and quick-fix components. The main body is integrally formed from a transparent anti-fog window and flexible edging; the airtight fit structure uses a three-section silicone sealing gasket combined with a micro-groove air-guiding structure to achieve a 360° low-leakage seal; the dual-path independent airflow module achieves physical separation of inhaled and exhaled air; the multi-stage filtration unit includes a pre-filter layer, a high-efficiency nano-filter layer, a virus inactivation layer, and a deodorizing adsorption layer; the external air supply interface can be connected to a vehicle-mounted air supply system, achieving unlimited endurance and automatic air source switching. This invention solves the problems of poor airtightness, low filtration efficiency, breathing difficulties, limited endurance, and inconvenience in emergency situations found in existing face shields, and is suitable for various scenarios such as infectious disease control, fire escape, and vehicle-mounted emergency response.

The present invention relates to methods of viral inactivation with a mixture of solvent and detergent. In particular, the present invention is directed to a method for preparing a virus-inactivated polypeptide solution wherein a polypeptide material is first loaded into an affinity chromatography column then incubated with said mixture of a solvent and a detergent within the affinity chromatography column.

The invention discloses an HPV virus inactivation dressing and a preparation method thereof, and relates to the field of HPV virus inactivation dressings, the HPV virus inactivation dressing comprises, by weight, 50-90 parts of animal extract, 5-15 parts of morchella, 5-10 parts of sodium carboxymethyl cellulose, 5-10 parts of gelatin and 30-35 parts of purified water. Through the synergistic effect of the animal extract, the morchella esculenta and special substances, HPV viruses can be efficiently inactivated, and the detergent has sterilization and anti-inflammation effects and is suitable for various detergent products; through the synergistic effect of the animal extract and the morchella esculenta, the HPV virus can be efficiently inactivated, and experimental results show that the inactivation rates of HPV16 type pseudoviruses and HPV18 type pseudoviruses reach 90.53% and 98.79% respectively.

The invention relates to the technical field of furniture design, in particular to a bed with a negative oxygen ion releasing function, which comprises a bed body, a mattress, a negative ion bed sheet and a negative ion latex mattress, the mattress is fixedly mounted at the top of the bed body, the negative ion bed sheet is laid at the top of the mattress, and the negative ion latex mattress is embedded in the mattress; pine needle extraction spray is evenly sprayed on the surface of cloth of the anion bed sheet, and the core component of the pine needle extraction spray is natural pine needle extract. According to the pine needle extraction spray, the plant ecological level negative oxygen ion technology is adopted, negative oxygen ions are passively and continuously released, the concentration of the negative oxygen ions in the sleep environment is stabilized to be 1000 pieces / cm or above, the health threshold value for improving immunity is achieved, the released negative oxygen ions can adsorb and settle particulate matters such as PM2.5 and dust, meanwhile, external structures of bacteria and viruses are destroyed, and the effect of improving the sleep quality is achieved. The sterilization rate on escherichia coli, staphylococcus aureus and the like is more than 99%, and the inactivation rate on new coronavirus reaches 99.96%. The health preserving effect is realized.

Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange / hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration / diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.

The utility model belongs to virus inactivation technical field discloses a kind of continuous online virus inactivation, incubation system, including sample stirrer, first delivery pump, first inactivator storage tank, second delivery pump, mixed stirrer, first four-way valve, concentration module, third delivery pump, first online detection module, second four-way valve, incubation module, second online detection module and finished product collection tank.Sample in sample stirrer and inactivator in first inactivator storage tank are continuously delivered to mixed stirrer according to required proportion, after mixing, it can be selected to enter concentration module concentration and enter first online detection module or directly enter first online detection module and carry out detection, the solution after detection can be selected to enter incubation module, after incubation, enter second online detection module or directly enter second online detection module and carry out detection, after detection, enter finished product collection tank and collect.The continuous online virus inactivation, incubation system can realize the automation of virus inactivation and incubation.

The inventions provide improved systems and methods for inline concentrating and reducing intermediate process pools to mitigate plant fit challenges associated with high-titer protein purification process. The inventions also provide methods for concentrating and reducing VI pool volumes by integrating columns of single pass tangential flow filtration (SPTFF) system with viral inactivation pool filtration (VIPF) in protein purification process. Therapeutic proteins purified by the methods also are provided.

The invention provides a virus inactivation method of a liquid biological product, which adopts a virus inactivation device to carry out virus inactivation on the liquid biological product, and the virus inactivation device comprises a box body, a console, an overflowing container arranged in the box body and an ultraviolet lamp group arranged in the box body, a liquid biological product is continuously introduced into the liquid inlet buffer bin through the peristaltic pump, upwards overflows through the liquid inlet buffer bin, enters the temporary liquid storage bin after passing through the plurality of quartz round tubes, and is finally discharged from the second flow guide silicone tube; the liquid biological product is irradiated by the first ultraviolet lamp and the second ultraviolet lamp to inactivate viruses in the process of flowing in the quartz round tubes. According to the invention, the virus inactivation effect can be improved while the biological activity of the liquid biological product is ensured, and continuous and stable virus inactivation of the liquid biological product can be realized. The virus inactivation device is easy to clean and maintain and long in service life, and the virus inactivation method based on the virus inactivation device is more economical, simpler and more convenient and suitable for large-scale treatment of liquid biological products.

The invention relates to a method for separating and purifying fibrinogen with low impure protein, and belongs to the technical field of blood product production. The method comprises the following steps: preparing a raw material component I, dissolving and filtering an FI precipitate, dissolving with a dissolving solution A, and filtering to obtain a filtrate A; purifying and separating polyethylene glycol, adjusting the pH value of the filtrate A, adding polyethylene glycol to precipitate, dissolving the precipitate by using the dissolving solution B, and filtering; s / D inactivation: adding an S / D solution into the filtrate B for virus inactivation; ethanol purification and separation: adding ethanol to the inactivated solution to precipitate protein, dissolving the precipitate with a dissolving solution B, and filtering; purifying and separating glycine, adding glycine precipitation protein into the filtrate C, dissolving the precipitate by using the dissolving solution C, and filtering; carrying out ultrafiltration dialysis, and dialyzing and concentrating the filtrate D; preparation: diluting the stock solution to a proper concentration; the semi-finished product is subpackaged, freeze-dried and subjected to dry heat inactivation treatment. The method can effectively remove impure proteins, improve the purity of fibrinogen and ensure the product safety.

This provides a method for developing an acid-based inactivation protocol for viruses in mixtures. [Solution] A method comprising: preparing a pool of eluate samples of a mixture containing a target molecule purified by a chromatography process; generating a pool of acidified samples having a pH below a first inactivation pH; generating a first data point for the acidified samples; generating a second data point for the inactivated samples; and using the first and second data points, regressing the relationship between the protein concentration of the mixture, the pH of the mixture, the inactivation pH range, and the amount of acid required to bring the mixture into the inactivation pH range.

Detergents for use to inactivate enveloped viruses, including detergents that are considered eco-friendly that can be used in biomanufacturing operations.

The present disclosure provides surfactant-based compositions, methods, kits, and systems for use in cell permeabilization, analytical and diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. In certain aspects, permeabilization buffers comprising defined concentrations of nonionic surfactants in polar protic solvents permeabilize diverse cell types while preserving intracellular targets and compatibility with downstream detection chemistry and nucleic acid amplification. Related compositions include loading, blocking, running, and mountant buffers, as well as polypeptide stabilization and viral inactivation solutions formulated with the surfactants. In additional aspects, the surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows, including determination of encapsulation efficiency. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes of buffer performance and recommend protocol parameters.

To provide a human norovirus inactivating agent and a sanitary material excellent in human norovirus inactivating effect.SOLUTION: A human norovirus deactivator comprising: (a) ethanol; and (b) at least two acids selected from the group consisting of gluconic acid, fumaric acid, lactic acid, malic acid, succinic acid, citric acid, and phosphoric acid, wherein the human norovirus deactivator has a pH of 1 to 6.SELECTED DRAWING: None

The application discloses a virus inactivation method in a recombinant human interleukin 10 (IL-10) purification process and application thereof. The pH of a sample containing the recombinant human IL-10 is adjusted to 7.0-9.0, mixed with a virus inactivator, and subjected to inactivation treatment, and the sample after the inactivation treatment is subjected to chromatography treatment by using a weak cation exchange column; the virus inactivator contains triphosylbutane and Tween-80. The application designs a new virus inactivation method, does not affect the physical and chemical stability and biological activity of IL-10, and has a virus inactivation index greater than or equal to 5 log values, can achieve inactivation effect in 2h, shortens the inactivation time of the sample, can effectively remove the organic reagents TNBP and Tween-80, and has a residual amount lower than the international standard requirement, and can also remove IL-10 monomer impurities generated by hydrolysis of IL-10 in the purification process, and improve the purity of the IL-10 sample.

The present application relates to the technical field of biological medicine, and particularly discloses a virus inactivation method and application. The virus inactivation method of the present application uses formaldehyde as an inactivator, and further includes a formaldehyde blocking step after the virus inactivation with formaldehyde. The active ingredients of the blocking agent used in the formaldehyde blocking step are sodium bisulfite and EDTA-2Na, and the mass ratio of the sodium bisulfite and the EDTA-2Na is (3.8-4.2):1. The method of the present application can effectively reduce the toxicity of formaldehyde when formaldehyde is used as an inactivator, and reduce the residual amount of formaldehyde, and is particularly suitable for the inactivation of bovine dermatophilus virus and the blocking of formaldehyde.

The invention provides an anti-virus medical mask and a preparation method and application thereof.The anti-virus medical mask comprises at least one filter layer, and a virus inactivation polymer coating is anchored to the surface of the filter layer through covalent bonds; the virus inactivation polymer is formed by polymerizing a (methyl) acrylamide monomer containing a tertiary amine group or a (methyl) acrylate monomer containing a tertiary amine group and a cross-linking agent containing a long alkyl hydrophobic chain segment. The medical mask can rapidly inactivate respiratory viruses, is safe and durable, keeps good air permeability, and can be used for daily protection of respiratory viruses or clinical environment protection.

A continuous flow reactor having a plurality of interwoven flow paths in fluid communication to form a single continuous flow reactor tube having a single flow path. 20 25 20 20 02 19 M ar 2 02 5 A B S T R A C T 2 0 2 5 2 0 2 0 0 2 1 9 M a r 2 0 2 5

The application relates to the field of biological medicine, and discloses a pH response phase transition sulfonic acid liposome for destroying a virus envelope structure as well as a preparation method and application thereof. The liposome is composed of dioleoyl phosphatidyl ethanolamine, (2,3-dioleoyl-propyl)-trimethylammonium chloride, oleic acid, sodium hexadecyl sulfonate and cholesterol. The liposome constructed by the application can neutralize viruses in the form of adhesion and wrapping of viruses in a high-pH environment in the early stage of virus infection, is endocytosed by cells, and is phase transitioned in lysosomes to realize intracellular inactivation of the viruses. In a low-pH microenvironment of inflammatory tissues caused by the late stage of virus infection, the liposome can directly destroy the virus envelope to realize virus inactivation through phase transition outside cells after adhesion of the viruses. Therefore, the liposome can realize effective killing of viruses in the early stage and the late stage of virus infection, has a spectrum antiviral effect, and has a wide application prospect.

The invention discloses a virus inactivation evaluation method based on image recognition and data processing, and relates to the technical field of biotechnology and medical detection, and the method comprises the following steps: preprocessing a collected microscopic image, and adopting a gray conversion and contrast adjustment method to enhance the light and shade difference between virus particles and polysaccharide residues in the image, so as to obtain a virus inactivation evaluation result; obtaining first image data after primary processing; according to the virus inactivation evaluation method based on image recognition and data processing, through extraction and analysis of morphological characteristics such as boundary integrity and shape regularity of virus particles, objective judgment of a virus inactivation state is realized, so that uncertainty caused by manual subjective judgment is reduced, misjudgment and missed judgment are reduced, and the accuracy of virus inactivation evaluation is improved. The accuracy, the stability and the repeatability of a virus inactivation evaluation result are improved.

A continuous flow reactor having a plurality of interwoven flow paths in fluid communication to form a single continuous flow reactor tube having a single flow path.