158 results about "Virus inactivation" patented technology

The invention belongs to the technical field of medical treatment, and discloses a blood product virus inactivation system based on a synergistic effect of nano-catalysis photochemical inactivation and low-temperature plasma, the system comprises: a nano-photocatalysis inactivation module adopting titanium dioxide TiO2 and graphite phase carbon nitride g-C3N4 nano-materials as photocatalysts; the low-temperature plasma inactivation module adopts a low-temperature plasma generator to generate plasma cloud above the blood product and release active particles; the dynamic fluid stirring module is used for enabling the blood product to uniformly receive photocatalysis and plasma inactivation effects through a microfluid mixing technology so as to ensure the uniformity of virus inactivation; the sensing monitoring and intelligent feedback module is used for monitoring the residual quantity of viral nucleic acid and the activity of protein in the blood in real time by adopting a spectrum sensor and a biochemical sensor. The method can be widely applied to the fields of clinical blood transfusion, blood product manufacturing, blood disease treatment and the like, promotes the progress of a blood safety guarantee technology, reduces the virus infection risk and improves the patient safety.

The invention relates to the technical field of repair materials, and particularly discloses an extracellular matrix fiber material as well as a preparation method and application thereof. The invention discloses a preparation method of an extracellular matrix fiber material. The preparation method comprises the following steps: taking animal tissues, and carrying out virus inactivation, decellularization and antigen removal, degreasing, drying, grinding and sterilization; the decellularization and antigen removal method comprises the following specific steps: sequentially soaking a sample with a surfactant solution for h; then, soaking treatment is carried out by adopting a salt-alkali solution which contains 0.05-3 mol / L of salt and has the pH value of 8-11; then carrying out soaking treatment by adopting a 0.1-1wt% enzyme solution; and in degreasing, one or more of normal hexane, trichloromethane, dichloromethane, methanol, ethanol, acetone, diethyl ether and ethyl acetate are used for soaking the sample. According to the technical scheme, the ECM powder particle material with high growth factor content is prepared.

The present invention provides a method for inactivating viruses in liquid biological products. The method uses a virus inactivation device to inactivate viruses in liquid biological products. The virus inactivation device includes a feeding part, a centrifugation part, and a discharging part. The centrifugation part is provided with an ultraviolet lamp. The liquid biological products are continuously fed into the centrifugation part through the feeding part. Under the action of centrifugation, the liquid biological products are thinned to a liquid layer thickness of 0.01 cm to 0.2 cm, and at the same time, the viruses are inactivated by ultraviolet irradiation through the ultraviolet lamp. After the viruses are inactivated, the liquid biological products are continuously discharged from the centrifugation part to the discharging part under the action of centrifugation, and are discharged from the virus inactivation device through the discharging part. The retention time of the liquid biological products in the centrifugation part is controlled to be 5 to 60 s, the irradiation intensity of the ultraviolet lamp is 500 to 10,000 μW / cm<supgt;2< / supgt;, and the irradiation wavelength of the ultraviolet lamp is 254 to 280 nm. The present invention is a continuous method for inactivating viruses in liquid biological products.

The invention belongs to the technical field of rabies vaccines, and particularly relates to a purification method of a rabies virus inactivated vaccine, which comprises the following steps: step 1, carrying out passage by using a Walvax-2 cell strain through a cell factory method, and carrying out multiplication culture on the passage production cells in a microcarrier bioreactor; 2, cleaning the cells with a treating fluid, inoculating the cells with a rabies virus fixed strain, and preparing and culturing a rabies virus solution; step 3, carrying out ultrafiltration concentration on the rabies virus stock solution; step 4, purifying the rabies virus concentrated solution; step 5, inactivating the rabies virus; and 6, hydrolyzing to obtain the rabies vaccine stock solution. By adopting the composite Capto Core 700 column chromatography, impure proteins can be effectively removed, the recovery rate is high, the quality of the rabies vaccine obtained by adopting a mode of firstly purifying and then adopting beta-propiolactone inactivation is excellent, and the effect of the finally obtained human diploid cell rabies vaccine stock solution is good.

The invention relates to a preparation method and application of a human immune globulin preparation stock solution, the preparation method comprises the steps of pretreatment of an initial raw material, ultrafiltration concentration and dialysis, and virus inactivation and filtration, the ultrafiltration concentration and dialysis process is completed under the participation of a dialysis buffer solution composed of a main functional component L-proline, and the human immune globulin preparation stock solution is obtained. The L-proline is used for effectively protecting human immune globulin in the preparation process of the human immune globulin preparation stock solution and the preparation, so that the influence of various unstable factors possibly causing polymerization of the human immune globulin product in the preparation process of the human immune globulin product in the prior art is effectively overcome; therefore, the stability and the quality of the human immune globulin preparation stock solution and the prepared product are effectively improved, and compared with the prior art, the technical scheme provided by the invention is simple, convenient and practical, and can achieve an extremely good technical effect, so that the human immune globulin preparation has outstanding beneficial effects and remarkable progress, and has extremely high popularization and application values.

The invention relates to the field of ultraviolet sterilization systems, in particular to an integrated ultraviolet sterilization system utilizing a UVC deep ultraviolet chip, which comprises a UVC deep ultraviolet chip lamp bead module serving as a core light source to emit deep ultraviolet light for sterilization; the self-adaptive heat dissipation module is attached to the UVC deep ultraviolet chip lamp bead module through heat conduction silicone grease and used for dynamically guiding out heat of the UVC deep ultraviolet chip lamp bead module; and the optical power closed-loop driving module is electrically connected with the UVC deep ultraviolet chip lamp bead module and is used for outputting stable current and performing feedback regulation according to optical power. According to the integrated ultraviolet sterilization system using the UVC deep ultraviolet chip, the high efficiency is remarkably improved: the NPSS + superlattice insertion layer enables the electro-optical conversion efficiency to be improved to more than 8%, and the deep ultraviolet light of 265-275 nm is 1t; the inactivation rate of escherichia coli and influenza viruses within 1 minute reaches 99.99%, and the disinfection and killing efficiency is improved by 30% compared with that of existing equipment.

The present invention discloses a preparation method of an active injectable gel that can withstand irradiation sterilization. The active injectable gel is composed of a collagen gel extracted from an animal source, acellular dermal matrix microparticles, bacterial cellulose, glycerol, and amino acids compounded in a certain proportion, and serves as a filling material that plays an exogenous filling and endogenous collagen regeneration role. Through the protection of glycerol and amino acids and the method of cryopackaging with liquid nitrogen or dry ice, the collagen gel can still maintain its activity and gel properties after being sterilized by Co60 irradiation, and achieve the effects of sterility and virus inactivation. By adding acellular dermal matrix microparticles and bacterial cellulose, the injection force is increased and the action time of the pure collagen gel is effectively extended to achieve a long-term effect.

The invention belongs to the field of protein purification, and relates to a method for preparing bevacizumab, which comprises the steps of affinity chromatography, virus inactivation, deep filtration, composite anion exchange chromatography, cation exchange chromatography and the like.

The invention relates to a method for inactivating viruses in a fluid, in which a starting fluid (AF) laden with an active virus is mixed in a first mixing device (2) with a virus inactivation reagent (R1) to form a reactive fluid (F) and the active virus is inactivated by incubating the reactive fluid (F), and stopping the inactivation of the virus in the reactive fluid (F) by mixing the reactive fluid (F) with another reagent (R2) or by removing the virus inactivation reagent (R1) from the reactive fluid (F), thereby producing the resulting fluid (RF). It is proposed that the active virus is inactivated by incubating a reactive fluid (F) in a container device (3) different from the first mixing device (2) and comprising a plurality of separate containers (4), the reactive fluid (F) being guided from the first mixing device (2) to the container device (3) before incubation.

The application discloses a kind of enveloped virus inactivation preparation based on medium-chain triglyceride and application thereof, the present application relates to the technical field of biological medicine external preparation, including active ingredient and auxiliary material: the active ingredient includes: high-purity medium-chain triglyceride (MCT), preferably octanoic acid (C8) and capric acid (C10) triglyceride mixture, purity ≥99%; The auxiliary material includes: food-grade antioxidant, for preventing oxidation of preparation, the content of food-grade antioxidant is 0.01-0.05%w / w, with medium-chain triglyceride as active ingredient, through the physical and chemical synergistic effect, enveloped virus is efficiently killed, for treating stubborn skin mucosa infection diseases (such as stubborn flat warts, herpes labialis, genital herpes, etc.) caused by human papillomavirus (HPV), herpes simplex virus (HSV-1 / HSV-2), varicella-zoster virus and other enveloped viruses.

Embodiments of the present disclosure describe a system and method for controlling the pH of a polypeptide-containing mixture. The pH may be controlled for any suitable purpose, for example, to inactivate a virus in the mixture. The method may include the step of eluting a mixture (e.g., an eluent) having a pH greater than, for example, 3.9 and, for example, less than, 8.5 from a chromatography column. The method may further include one or more of the steps of measuring the protein concentration of the mixture and measuring the pH of the mixture. Subsequently, the amount of acid required to reduce the pH of the mixture to a target pH may be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After calculating the amount of acid to be added, a portion of the acid may be added to the mixture, wherein said portion of the acid is sufficient to achieve the target pH.

The application relates to a double-metal hydroxide, in particular to application of a CuAl double-metal hydroxide, in particular to application of the CuAl double-metal hydroxide in preparation of a preparation for inactivation of pathogenic bacteria, fungi and viruses. The CuAl double-metal hydroxide is prepared by a formamide liquid phase stripping method. Compared with the prior art, the application solves the problem of high cost of a silver ion loaded layered double-metal hydroxide in the prior art, and realizes preparation of a low-cost, high-performance and green antibacterial material with a bactericidal and antiviral effect.

The invention relates to a cherry valley duck PLV virus vaccine as well as a preparation method and application thereof, and belongs to the technical field of virus immunity. The vaccine is prepared by taking a Parry's Lagon virus (PLV) virus strain PLV-20155 as an immunogen, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.46296. The invention further discloses a preparation method of the vaccine. The vaccine is a PLV-20155 virus inactivated vaccine, and the preparation method of the vaccine comprises the following steps: inoculating the PLV-20155 virus into an SPF chick embryo for propagation, harvesting a virus liquid, inactivating with formaldehyde, verifying safety, emulsifying with white oil to prepare the vaccine and the like. In addition, the vaccine disclosed by the invention can be used for effectively preventing diseases such as laying rate reduction, follicle necrosis and fallopian tube atrophy of the cherry valley ducks caused by the PLV-20155 virus, and the economic benefit of breeding is remarkably improved.

The present invention relates to the field of biomedicine, and more particularly to a method for purifying an inactivated rabies virus vaccine. The method comprises the following steps: preparing an inactivated rabies virus ultrafiltration concentrate: purifying the inactivated rabies virus ultrafiltration concentrate by chromatography; the chromatography purification specifically comprises the following steps: column cleaning: loading a composite filler into a chromatography column, connecting an AKTA to the chromatography column, cleaning, sterilizing, and setting aside; chromatography operation: equilibrating the chromatography column with PBS buffer; sample loading: loading the inactivated rabies virus ultrafiltration concentrate into the chromatography column, eluting with an eluent, and collecting the eluate corresponding to the absorption peak at an ultraviolet wavelength of 280 nm, which is the purified inactivated rabies virus vaccine; column cleaning; and results showing that the residual protein content is as low as 5.0 ng / ml, the antigen content can reach above 16.0 IU / ml, the host protein removal rate can reach above 99%, and the antigen content recovery rate can reach above 86%.

The application discloses a preparation method of a tetravalent influenza virus split vaccine and belongs to the technical field of vaccines. The preparation method of the tetravalent influenza virus split vaccine comprises the following steps: pre-incubation of a chicken embryo, pre-embryo inspection, virus inoculation and culture, post-embryo inspection, cold embryo, virus harvesting, ultrafiltration clarification, zone centrifugation, desugaring, chromatography, virus splitting, virus inactivation, ultrafiltration liquid replacement, sterilization filtration and preparation of a tetravalent influenza virus split vaccine product. Compared with the prior art, the technical means adopted in the application effectively reduces the content of free formaldehyde and improves the antibody level of the vaccine.

The utility model discloses a frost prevention and dust removal device and heat exchange system based on plasma wind, including a plurality of plasma wind generation modules, the plurality of plasma wind generation modules are arranged in array, each plasma wind generation module includes an ionization unit and a high voltage conversion unit, the high voltage conversion unit is connected with a power supply, and the ionization unit is connected with the power supply. The high-voltage conversion unit is used for converting power supply voltage into high-voltage electricity needed by ionization, the ionization unit is connected with the high-voltage conversion unit, the high-voltage electricity generated by the high-voltage conversion unit is applied to the ionization unit to form a high-voltage electric field, gas molecules in air are ionized to form plasma wind, water molecule frosting can be restrained, a formed frost layer is decomposed, and the air quality is improved. Reverse circulation of the heat exchange system is not needed, the heating or refrigerating stability is improved, the service life of a compressor, a valve and other key components is prolonged, in addition, plasma air can further conduct dust removal, organic pollutant decomposition, sterilization and virus inactivation on the surface of the evaporator, the heat exchange efficiency can be improved, the operation and maintenance cost can be reduced, and the application range of the heat exchange system can be expanded.

The invention provides a method for predicting the ultraviolet inactivation effect of viruses in a liquid biological product. The method comprises the following steps: (1) detecting the absorbance value of a to-be-detected liquid biological product at a preset wavelength; (2) constructing a relation curve of the ultraviolet dose of the BSA / virus mixed solution and the decline degree of the virus titer; (3) constructing a relation curve of the ultraviolet dose of the SMZ working solution and the SMZ degradation degree; (4) constructing a relation curve of the operation parameters and the SMZ degradability; and (5) substituting preset operation parameters into the relation curve of the operation parameters and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the SMZ degradability, substituting the obtained result into the relation curve of the ultraviolet dose and the decline of the indication virus titer, and judging the ultraviolet inactivation effect of the virus according to a predicted value of the decline of the indication virus titer. The method is accurate in prediction result, does not need to consume liquid biological products, and can be used for various virus inactivation effects under the condition that the ultraviolet dose cannot be accurately calculated.

The invention relates to an application of a cerium oxide nano-material in a vaccine adjuvant and / or a vaccine inactivator, in particular to a vaccine, when the cerium oxide nano-material is the vaccine adjuvant, the vaccine comprises the vaccine adjuvant and an immunogen; or when the cerium oxide nano material is a vaccine inactivating agent, the vaccine is a composite vaccine, the composite vaccine comprises the vaccine inactivating agent and a virus, and the virus is embedded in the cerium oxide nano material; the preparation method of the composite vaccine comprises the step of mineralizing and inactivating viruses by using a vaccine inactivator, wherein the vaccine inactivator is cerium salt. The cerium oxide can be used as a vaccine adjuvant and also can be used as a virus inactivator. The maturation of dendritic cells and macrophages can be promoted by means of regulating the level of active oxygen in immune cells and the like, and the antigen presentation efficiency is improved, so that the vaccine-induced body fluid and cellular immune response is enhanced; meanwhile, cerium salt can directly act on virus surface protein for mineralization, efficient and safe virus inactivation is achieved, and preparation of inactivated vaccines is facilitated.

The invention provides a biological product virus inactivation device and application thereof.The biological product virus inactivation device comprises an outer cylinder support, an outer cylinder, an inner cylinder, a collecting bin, an ultraviolet lamp and a driving device, the outer cylinder is arranged on the outer cylinder support and is of a hollow structure with the two ends open, and the axis of the outer cylinder is obliquely arranged relative to the horizontal plane; the inner cylinder is rotatably arranged in the outer cylinder around the axis of the inner cylinder, the inner cylinder is of a hollow structure with two open ends, and the axis of the inner cylinder coincides with the axis of the outer cylinder; the opening of the collecting bin is positioned below the lower end opening of the inner cylinder; the ultraviolet lamp is a strip-shaped lamp and is fixedly arranged in the inner cylinder or between the outer wall of the inner cylinder and the inner wall of the outer cylinder; the driving device is arranged between the outer cylinder and the inner cylinder and can drive the inner cylinder to rotate around the axis of the inner cylinder. The biological product virus inactivation device is more convenient to operate, biological product feed liquid is not prone to bubbling in the virus inactivation process, the activity of a biological product can be better reserved, and the biological product virus inactivation device is suitable for large-scale industrial treatment.

A virus inactivation method is provided. The method includes irradiating a solution containing an artificially expressed antibody or antibody fragment with ultraviolet rays in the presence of a radical scavenger. The irradiating only aggregates 5% or less of the antibody or antibody fragment. A wavelength of the ultraviolet rays is 200 nm or longer and 315 nm or shorter. An irradiation amount of the ultraviolet rays is 300 mJ / cm2 or more. An irradiation time of the ultraviolet rays is 3 minutes or less. A concentration of the radical scavenger in the solution is 0.03 mM or more.

This invention discloses an emergency vehicle-mounted isolation face shield, belonging to the field of personal protective equipment technology. The face shield includes a main body, an airtight fit structure, a dual-path independent airflow module, a multi-stage virus filtration and purification unit, an external air supply interface, an anti-fog window, and quick-fix components. The main body is integrally formed from a transparent anti-fog window and flexible edging; the airtight fit structure uses a three-section silicone sealing gasket combined with a micro-groove air-guiding structure to achieve a 360° low-leakage seal; the dual-path independent airflow module achieves physical separation of inhaled and exhaled air; the multi-stage filtration unit includes a pre-filter layer, a high-efficiency nano-filter layer, a virus inactivation layer, and a deodorizing adsorption layer; the external air supply interface can be connected to a vehicle-mounted air supply system, achieving unlimited endurance and automatic air source switching. This invention solves the problems of poor airtightness, low filtration efficiency, breathing difficulties, limited endurance, and inconvenience in emergency situations found in existing face shields, and is suitable for various scenarios such as infectious disease control, fire escape, and vehicle-mounted emergency response.

To provide a virus inactivation composition having a high inactivating effect against non-enveloped viruses.SOLUTION: A composition for inactivating non-enveloped viruses, comprising (a) monoethanolamine, (b) an aromatic alcohol, and water, and having a pH of 10.5 or more and 14 or less at 25°C.SELECTED DRAWING: None

A virus inactivation device includes at least one experimental continuous virus inactivation reactor, the reactor having at least an inlet, an outlet and a tubular flow path, and a computer system that can design, select, make and / or manufacture a scaled actual reactor based on the experimental continuous virus inactivation reactor. The tubular flow path includes a set of alternating turns that form a serpentine or interweaving pattern between the inlet and the outlet.

The present invention relates to methods of viral inactivation with a mixture of solvent and detergent. In particular, the present invention is directed to a method for preparing a virus-inactivated polypeptide solution wherein a polypeptide material is first loaded into an affinity chromatography column then incubated with said mixture of a solvent and a detergent within the affinity chromatography column.

The application relates to a method for inactivating viruses in sewage. The method comprises the following steps: introducing heterotrophic bacteria into sewage to be inactivated; increasing the carbon-nitrogen ratio of the sewage during the activation and expansion of the heterotrophic bacteria; washing and resuspending the activated and expanded heterotrophic bacteria to form a heterotrophic bacteria liquid to be used; inoculating the heterotrophic bacteria liquid into the sewage and reducing the carbon-nitrogen ratio of the sewage, so that the heterotrophic bacteria produce an oxidative stress response to inactivate viruses. The method adjusts the carbon-nitrogen ratio to make the heterotrophic bacteria change from an eutrophic environment to an oligotrophic environment, activates the oxidative stress pathway of the heterotrophic bacteria, increases the generation of oxidative active species such as superoxide free radicals, causes oxidative damage to the virus shell or envelope, and thus promotes the inactivation of viruses. The method is simple and efficient, and has a wide application scenario.

The invention belongs to the technical field of biomedical materials, and particularly discloses a method for inactivating viruses of a valve leaflet material. Comprising the following steps: (1) immersing fixing cloth by using a glutaraldehyde solution until the fixing cloth is fully infiltrated, unfreezing bovine pericardium sheets subjected to an anti-calcification treatment process, draining off liquid on the surfaces, and paving the bovine pericardium sheets by using the fixing cloth to obtain a layer of cloth, a layer of bovine pericardium sheets and a layer of cloth for later use; (2) cross-linking immobilization-1: putting the bovine pericardium slices treated in the step (1) into a box, pouring a glutaraldehyde solution, enabling the glutaraldehyde solution to fully infiltrate the bovine pericardium slices, enabling the height of the glutaraldehyde solution to be flush with the height of the uppermost layer of fixing cloth, and refrigerating to obtain an intermediate product; and (3) EO treatment: carrying out ethylene oxide sterilization on the intermediate product treated in the step (2) to obtain a treated product. Through cross-linking fixation and EO sterilization treatment, the advantages of thorough virus inactivation and small damage to the structure and performance of the valve leaflet material are realized.

The present invention discloses a rabies virus serum-free Vero cell inactivated vaccine and a preparation method thereof, and belongs to the technical field of vaccine manufacturing. The preparation method of the inactivated vaccine comprises the following steps: resuscitating Vero cells, performing serum-free adaptation and domestication of the Vero cells, preparing modified microcarriers, adding the modified microcarriers to a bioreactor, adding serum-free culture medium, inoculating the domesticated Vero cells into the bioreactor, amplifying and harvesting the cells, inoculating rabies virus, adding serum-free culture medium for virus culture, harvesting, and obtaining a virus liquid; finally, inactivating the virus, incubating, concentrating, purifying, and freeze-drying to obtain a rabies virus serum-free Vero cell inactivated vaccine. The virus liquid prepared by the present invention has the advantages of high virus content, stable production process, and low production cost, and the prepared vaccine has the effects of improving safety in use and reducing side effects.

The invention discloses an HPV virus inactivation dressing and a preparation method thereof, and relates to the field of HPV virus inactivation dressings, the HPV virus inactivation dressing comprises, by weight, 50-90 parts of animal extract, 5-15 parts of morchella, 5-10 parts of sodium carboxymethyl cellulose, 5-10 parts of gelatin and 30-35 parts of purified water. Through the synergistic effect of the animal extract, the morchella esculenta and special substances, HPV viruses can be efficiently inactivated, and the detergent has sterilization and anti-inflammation effects and is suitable for various detergent products; through the synergistic effect of the animal extract and the morchella esculenta, the HPV virus can be efficiently inactivated, and experimental results show that the inactivation rates of HPV16 type pseudoviruses and HPV18 type pseudoviruses reach 90.53% and 98.79% respectively.

The invention relates to the technical field of furniture design, in particular to a bed with a negative oxygen ion releasing function, which comprises a bed body, a mattress, a negative ion bed sheet and a negative ion latex mattress, the mattress is fixedly mounted at the top of the bed body, the negative ion bed sheet is laid at the top of the mattress, and the negative ion latex mattress is embedded in the mattress; pine needle extraction spray is evenly sprayed on the surface of cloth of the anion bed sheet, and the core component of the pine needle extraction spray is natural pine needle extract. According to the pine needle extraction spray, the plant ecological level negative oxygen ion technology is adopted, negative oxygen ions are passively and continuously released, the concentration of the negative oxygen ions in the sleep environment is stabilized to be 1000 pieces / cm or above, the health threshold value for improving immunity is achieved, the released negative oxygen ions can adsorb and settle particulate matters such as PM2.5 and dust, meanwhile, external structures of bacteria and viruses are destroyed, and the effect of improving the sleep quality is achieved. The sterilization rate on escherichia coli, staphylococcus aureus and the like is more than 99%, and the inactivation rate on new coronavirus reaches 99.96%. The health preserving effect is realized.