Method for purifying anti-HER2 or/and anti-HER3 antibody proteins
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A purification method and protein technology, applied in the field of biochemistry, to increase the virus filtration process, reduce production costs, and improve production efficiency
Active Publication Date: 2012-06-13
GENOR BIOPHARMA +1
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[0004] The technical problem to be solved by the present invention is aimed at the problems of existing anti-HER2 or/and anti-HER3 purification methods, and provides an anti-HER2 or/and
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[0024] Below in conjunction with specific embodiment, further illustrate the present invention. These examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the following examples, usually follow the conventional conditions or the conditions suggested by the manufacturer. Unless otherwise defined, all professional and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. In addition, any methods and materials similar or equivalent to those described can be applied to the method of the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only.
[0025] 1. Harvesting of Fermentation Supernatant
[0026] A two-stage depth filtering method is used.
[0027] Fermentation broth: the fermentation broth containing anti-HER2 monoclon...
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Abstract
The invention belongs to the biochemistry technical field, concretely relates to a method for purifying anti-HER2 or/and anti-HER3 antibody proteins. The method of the invention comprises the following the steps: clarifying a broth, carrying out affinity chromatography, carrying out inactivation on virus by acidic pH value, carrying out cation exchange chromatography, carrying out anion exchange chromatography; and filtering virus; wherein, the step of virus inactivation by acidic pH value and the step of virus filtration can be inserted at any position of the step after the affinity chromatography. The method of the invention comprises the following advantages that: 1) the chromatography step can be reduced to three steps, thereby the production efficiency can be enhanced and the production cost can be minimized. 2) the step of virus filtration is added, thereby the security can be raised.
Description
technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for purifying anti-HER2 or / and anti-HER3 antibody protein. Background technique [0002] With the development of biomedical technology, more and more mammalian cells are used to produce therapeutic recombinant proteins, such as monoclonal antibodies, cytokines, hormones, growth factors, and other enzymes, coagulation factors, etc. The treatment areas involved are becoming more and more extensive, including endocrinology, cardiovascular and cerebrovascular, surgery, blood diseases, anti-tumor and so on. [0003] In the production process of therapeutic recombinant proteins, people often have differences according to the size, shape, charge, hydrophobicity, solubility and biological activity of each protein. These differences are used by ion exchange (IEX), gel filtration ( GF), hydrophobic (HIC), reverse phase (RPC), affinity (AC) and other methods to se...
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