Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for purifying anti-HER2 or/and anti-HER3 antibody proteins

A purification method and protein technology, applied in the field of biochemistry, to increase the virus filtration process, reduce production costs, and improve production efficiency

Active Publication Date: 2012-06-13
GENOR BIOPHARMA +1
View PDF3 Cites 31 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is aimed at the problems of existing anti-HER2 or / and anti-HER3 purification methods, and provides an anti-HER2 or / and anti-HER3 purification method, so as to simplify the purification steps and improve the recovery rate of the target protein , and can remove the virus, reduce the risk of potential virus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for purifying anti-HER2 or/and anti-HER3 antibody proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] Below in conjunction with specific embodiment, further illustrate the present invention. These examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the following examples, usually follow the conventional conditions or the conditions suggested by the manufacturer. Unless otherwise defined, all professional and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. In addition, any methods and materials similar or equivalent to those described can be applied to the method of the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only.

[0025] 1. Harvesting of Fermentation Supernatant

[0026] A two-stage depth filtering method is used.

[0027] Fermentation broth: the fermentation broth containing anti-HER2 monoclon...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the biochemistry technical field, concretely relates to a method for purifying anti-HER2 or / and anti-HER3 antibody proteins. The method of the invention comprises the following the steps: clarifying a broth, carrying out affinity chromatography, carrying out inactivation on virus by acidic pH value, carrying out cation exchange chromatography, carrying out anion exchange chromatography; and filtering virus; wherein, the step of virus inactivation by acidic pH value and the step of virus filtration can be inserted at any position of the step after the affinity chromatography. The method of the invention comprises the following advantages that: 1) the chromatography step can be reduced to three steps, thereby the production efficiency can be enhanced and the production cost can be minimized. 2) the step of virus filtration is added, thereby the security can be raised.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a method for purifying anti-HER2 or / and anti-HER3 antibody protein. Background technique [0002] With the development of biomedical technology, more and more mammalian cells are used to produce therapeutic recombinant proteins, such as monoclonal antibodies, cytokines, hormones, growth factors, and other enzymes, coagulation factors, etc. The treatment areas involved are becoming more and more extensive, including endocrinology, cardiovascular and cerebrovascular, surgery, blood diseases, anti-tumor and so on. [0003] In the production process of therapeutic recombinant proteins, people often have differences according to the size, shape, charge, hydrophobicity, solubility and biological activity of each protein. These differences are used by ion exchange (IEX), gel filtration ( GF), hydrophobic (HIC), reverse phase (RPC), affinity (AC) and other methods to se...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/28C07K1/36C07K1/34C07K1/22C07K1/18
Inventor 徐志豪王威陈霖杰
Owner GENOR BIOPHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com