13results about How to "Increase synthesis" patented technology

The invention provides a stationary phase for DNA synthesis and a preparation method and application thereof. The preparation method of the stationary phase for DNA synthesis, provided by the invention, comprises the following steps: S1, preparing nano magnetic ferroferric oxide particles, and coating the surfaces of the nano magnetic ferroferric oxide particles with SiO2 layers to obtain a nano magnetic composite material Fe3O4-SiO2; s2, carrying out azide group modification on the surface of the nano magnetic composite material Fe3O4 (at) SiO2 to form a surface-modified nano magnetic composite material Fe3O4 (at) SiO2 (at) N3; and S3, loading the initiator to the surface of the surface-modified nano magnetic composite material Fe3O4 (at) SiO2 (at) N3 through Click reaction to obtain the stationary phase for DNA synthesis. The stationary phase provided by the invention can be used for DNA synthesis, and has the characteristics of high bonding strength, good stability, difficult shedding of initiators, high DNA synthesis yield and the like.

The invention belongs to the technical field of lithium ion battery positive electrode materials, and particularly discloses a composite positive electrode material of a solid electrolyte epitaxial coating, which comprises an inner-layer positive electrode active material and an epitaxial layer coated outside the inner-layer positive electrode active material, the inner-layer positive electrode active material is a configuration entropy ultrahigh nickel positive electrode material, and the general formula of the configuration entropy ultrahigh nickel positive electrode material is LiNi < x > Co < y > M < 1-x-y > O < 2 >. The disorder degree and inherent strain of the positive electrode material can be relieved through the chloride solid electrolyte epitaxial coating, the consistency and interface compatibility between an electrode and an electrolyte are enhanced, the electrochemical performance of the configuration entropy ultrahigh nickel positive electrode material LiNi < x > Co < y > Mn < 1-x-y > O < 2 > is improved, and therefore the composite positive electrode material with high stability and long service life is constructed.

The invention belongs to the technical field of microorganisms, and discloses preparation, separation and purification methods and application of lactobacillus casei IOB-P9 anti-helicobacter pylori active substance separation.The preparation, separation and purification methods of the active substance separationcomprises the following steps that lactobacillus casei IOB-P9 is cultured, and active ingredients are prepared; performing macroporous adsorption chromatography separation on the active ingredients; separating active components by an anion exchange method; performing gel filtration chromatography separation on the active ingredients; and verifying the activity of the active component to obtain the active component of the lactobacillus casei IOB-P9. Active substances in the lactobacillus casei IOB-P9 obtained according to the method have certain sensitivity to protease, which indicates that the bacteriostatic active substances are protein or peptide substances. In addition, the bacteriostatic active substance has better thermal stability, is not easy to dissolve in organic reagents and has long preservation time, so that the bacteriostatic active substance is beneficial to storage and transportation and has higher production and application values.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

The application belongs to the technical field of bioengineering, and discloses a gene engineering bacterium of acetobacter pasteurii with high temperature resistance, a construction method and application thereof. Acetobacter pasteurianus The gene engineering bacterium is constructed by using the acetobacter pasteurii (ATCC 10137) as a starting strain and knocking out the transposase gene in the genome of the acetobacter pasteurii (ATCC 10137) by a homologous recombination method. TnsR The gene engineering bacterium with high temperature resistance is obtained. TnsR The deletion of the gene is beneficial to maintaining the stability of the genome, so that the strain can maintain better growth and metabolic capacity under high-temperature pressure. Experimental results show that the biomass, survival rate and acetic acid yield of the gene engineering strain under the condition of 40 DEG C high temperature are significantly better than those of the wild-type strain. When the gene engineering strain is applied to traditional solid-state food vinegar brewing, the yield of organic acids such as acetic acid is significantly improved, the content of ester flavor substances is greatly improved, and the flavor quality of food vinegar is effectively improved. The application provides an effective technical means for solving the problem that high temperature in summer leads to abnormal fermentation and yield reduction of food vinegar.

The present invention discloses a ZSM-23 zeolite and a process for preparing the same and use thereof. The total acid amount of said ZSM-23 zeolite is 0.05-0.25 mmol / g, preferably, 0.06-0.22 mmol / g, more preferably, 0.06-0.20 mmol / g; the strong acid content of said ZSM-23 zeolite is 5-33%, preferably, 7-33%, more preferably, 9-33%, or further preferably, 7-31%, further more preferably, 10-28% of the total acid amount; wherein the strong acid refers to an acid having a desorption temperature of 350°C or higher in an NH3 temperature programmed desorption (NH3-TPD). The ZSM-23 zeolite has a low strong acid content, and the preparation process of the ZSM-23 zeolite is simple.

The invention belongs to the technical field of organic synthesis, and discloses a method for synthesizing an alkyl borate compound by taking allyl alcohol as a substrate, which comprises the following steps: by taking allyl alcohol as shown in a formula I and bis (pinacolato) diboron as substrates and cuprous salt as a catalyst, carrying out a reaction on the allyl alcohol and bis (pinacolato) diboron under the action of alkali, a proton source and a ligand to obtain the alkyl borate compound. And reacting in an organic solvent according to the following reaction formula to obtain the alkyl borate compound as shown in the formula II. The cuprous is adopted to catalyze the bis (pinacolato) diboron to be coupled with the allyl alcohol to synthesize the alkyl borate, so that the substrate range is widened, the use of noble metals and strong reducing agents is avoided, redundant metal wastes are not generated, the green chemistry concept is met, meanwhile, the reaction steps are remarkably shortened, and the cost can be reduced.

It is an object of the present invention to provide a chemical solution synthesis device that feeds a chemical solution free of contact with the atmosphere, wherein the efficiency of synthesizing carriers and a chemical solution in a reaction vessel can be improved. More specifically, this chemical solution synthesis device comprises a chemical solution holding unit holding a chemical solution, and a reaction vessel in which the chemical solution and carriers are reacted, in which the chemical solution is transferred from the chemical solution holding unit to the reaction vessel without coming into contact with the atmosphere, wherein the chemical solution synthesis device has a first chemical solution feed unit that is connected to the reaction vessel and supplies a chemical solution to the reaction vessel, and a chemical solution feed and discharge unit that is provided vertically above the first chemical solution feed unit, and there is provided a second chemical solution feed unit that knocks off any carriers adhering to the inner wall of the reaction vessel after the chemical solution has been discharged from the chemical solution feed and discharge unit.

The invention discloses a method for increasing the content of vinblastine in catharanthus roseus based on an EIN3 / EILs transcription factor CrEIL1 and a method for measuring the content of the vinblastine. The method comprises the following steps: cloning a Catharanthus roseus CrEIL1 gene, constructing a pLB-CrEIL1 plasmid vector, constructing a CrEIL1 overexpression vector by using the pLB-CrEIL1 plasmid vector, using the CrEIL1 overexpression vector, and performing agrobacterium tumefaciens-mediated overexpression of CrEIL1 in Catharanthus roseus, so that the yield of vinblastine in Catharanthus roseus can be increased in a low-cost, environment-friendly and reliable manner.

The invention relates to the technical field of artificial cultivation of medicinal fungi, and discloses a cultivation method of ganoderma lucidum spore powder with high triterpenoid polysaccharide content, which comprises the following steps: S1, inoculating a ganoderma lucidum strain in a seed solution culture medium, and culturing to obtain a seed solution; s2, inoculating the seed solution to a fermentation culture medium, and culturing to obtain a fermentation solution; s3, carrying out oscillation-standing circulation culture on the fermentation liquid, and separating the bacterial liquid to obtain mycelia; s4, inoculating the mycelium into a cultivar culture medium for culturing to obtain a ganoderma lucidum cultivar; s5, transplanting ganoderma lucidum cultivated species on the substitute culture medium, and culturing to obtain ganoderma lucidum; s6, the ganoderma lucidum is sleeved with a net cover, and ganoderma lucidum spore powder is collected. The content of triterpenes and polysaccharides in the ganoderma lucidum spore powder can be increased.

The invention discloses a bacillus licheniformis recombinant strain with high yield of L-cysteine, and belongs to the technical field of synthetic biology and microbial metabolism engineering. The cysteine synthesis flux is enhanced through multiple knockout and combined overexpression. An efficient engineering strain is constructed by knocking out a serine hydroxymethyltransferase coding gene glyA and an L-serine ammonolysis enzyme coding gene sdaAA of a serine catabolism key pathway, knocking out a cysteine catabolism gene metC and combining gene overexpression to introduce a feedback inhibition removal mutant cysEf and a transporter eamA, and the efficient engineering strain is used for preparing a recombinant strain. Finally, the yield of the L-cysteine reaches 1.075 g / L in shake flask fermentation (the conversion rate is 18.69%), and the application potential of the bacillus licheniformis in sulfur metabolism engineering is highlighted.

The present invention relates to a preparation method of Lactobacillus paracasei IOB413 bacterial powder for regulating butyric acid synthesis and its application in the immune direction, comprising activating Lactobacillus paracasei IOB413 strain; inoculating the activated strain into an optimized culture medium for culturing to obtain a secondary seed liquid; inoculating the secondary seed liquid into a sterilized fermentation broth medium for fermentation; centrifuging the fermentation broth medium, collecting bacterial mud, freeze-drying the bacterial mud, and preparing Lactobacillus paracasei IOB413 bacterial powder. The present invention can significantly increase the amount of butyric acid synthesized by Lactobacillus paracasei IOB413 in the intestine by optimizing the culture medium, and further elaborates in detail that Lactobacillus paracasei IOB413 affects intestinal function by promoting butyric acid synthesis, and finally exhibits the effects of enhancing immunity, repairing damaged immune organs, improving intestinal damage, regulating intestinal microecology, etc.

The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence selected from SEQ ID NOs 10-35, or a functionally equivalent sequence, with at least an amino acid substitution at the position corresponding to residue C302 (with respect to SEQ ID NO: 1) or a functionally equivalent residue in SEQ ID NOs 10-35, respectively, (ii) is able to synthesize a nucleic acid fragment without a template and (iii) is able to incorporate a 3'-O-modified nucleotide into the nucleic acid fragment.