41 results about "Catharanthus" patented technology

A plant activator composition increases the concentration of terpenes a terpinoids in aromatic plant oils, and hence resulting in an increased concentration of terpene and terpinoids in the harvested dried plant or fruit. The composition contains one of more bio-active compounds that are optionally extracted from plants selected from one or more of the group consisting of mango, citrus (including grapefruit), Catharanthus roseus and Pelargonium odoratissimum, but alternatively may include one or more synthetic compounds selected from the group consisting of geranyl acetate, geraniol, beta-sitosterol, alpha-amyrin, beta amyrin, carotenoid, geranyl acetate, alpha-humulene, mevalonate kinase and geranyl. Depending on the type of plant being treated, the formulation is added during watering and feeding in optimum doses during the vegetative growth, flowering, and fruit set and / or swell stages.

The invention relates to the technical field of bioengineering and discloses a method for improving camptothecin content in hairy roots of the new-type medicine source plant ophiorrhiza pumila of camptothecin. The method disclosed by the invention comprises the following steps of: cloning coding frame sequences of genes of ophiorrhiza japonica strictosidine synthase and geraniol-10-hydroxylase in catharanthus roseus to build a plant bivalent efficient expression vector containing the genes, carrying out genetic transformation on ophiorrhiza pumila via an agrobacterium rhizogenes mediated method to acquire the hairy roots of the ophiorrhiza pumila for transforming CrSTR and CrG10H genes; and inducing and treating high-yield camptothecin strains by adopting plant hormones to acquire high-yield hairy roots with the camptothecin content of 4.703mg / g DW. The MTT (Methyl Thiazolyl Tetrazolium) detection proves that camptothecin crude extract acquired via a transgenosis manner is good in biological activity and the lethality to cancer cells reaches 35.9%. By adopting the method disclosed by the invention, a new medicine source for acquiring the camptothecin is provided and a new method for producing anti-cancer medicine camptothecin in important clinical demand is provided.

The invention discloses a method for producing catharanthus roseus alkaloid. The method comprises the following steps: firstly, dedifferentiation culture is performed on the explant of the catharanthus roseus to get the callus of the catharanthus roseus, and through succesive transfer culture, loose and quick-growing cell line is obtained; secondly, the cell line of the callus of the catharanthusroseus obtained in the step one is processed through inducing the culture in basal culture medium provided with colchicines to get polyploidy cell line, and then expanding culture is performed to geta large amount of catharanthus roseus polyploidy cells; thirdly, the catharanthus roseus polyploidy cells are inoculated into a deffined medium to get cells being rich ofcatharanthus roseus secondarymetabolites. The method of the invention produces the catharanthine with the catharanthus roseus, the industrial production of the catharanthine can be firmly realized, the production cost is low, theculture period is short, the production is not influenced by natural environment and climate, and the year-round production can be realized.

A method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien comprises the steps as follows: taking aseptic seedling of the catharanthus roseus, and obtaining hypocotyl of the aseptic seedling; preparing bacteria solution of the agrobacterium tumefacien and utilizing the agrobacterium tumefacien for inducing transformation of the hypocotyl of catharanthus roseus; taking and placing the hypocotyl on a callus induction culture medium for culture to obtain a callus; inoculating the callus onto a differential culture medium for culture to obtain a green callus; inoculating the green callus onto an indefinite bud induction culture medium for culture to obtain an indefinite bud; and taking and placing the indefinite bud into a radication culture medium for culture to obtain the catharanthus roseus transgenic plants. In the method, a stable genetic transformation system of the catharanthus roseus induced by the agrobacterium tumefacien is established, therefore the method lays a foundation for further study on metabolic pathway control net and for the improvement of TIAs content, and can be further applied to gene engineering seed breeding for producing TIAs metabolite.

The invention discloses a method for improving the transplanting survival rate of a catharanthus roseus tissue culture seeding. The method is characterized by comprising the following steps of: (1) selecting strongly and regularly grown catharanthus roseus aseptic test-tube seedling, opening a cover of a culture flask, adding the suitable amount of distilled water to the culture flask to immerse the root of the catharanthus roseus, and putting the catharanthus roseus seedling in an illumination incubator to culture for 3-4d to finish a seedling hardening operation; (2) taking out the catharanthus roseus aseptic test-tube seedling from the culture flask by a pair of tweezers, flushing a culture medium of the root of the catharanthus roseus aseptic test-tube seedling cleanly by running water, putting the culture medium in a test tube or a culture dish containing a water-culture nutrient solution, putting the test tube or the culture dish in the illumination incubator to continue the seedling hardening operation, and finishing the water culture when a new water root is grown up from the root of the catharanthus roseus plant after the catharanthus roseus plant is cultured for 2-7d; and (3) transplanting the newly grown catharanthus roseus water-culture plant in a transplanting medium containing vermiculite, perlite and turfy to finish the transplanting of the catharanthus roseus tissue culture seeding. The method for improving the transplanting survival rate of the catharanthus roseus tissue culture seeding disclosed by the invention has the advantages of reinforcing the antibacterial ability of the catharanthus roseus tissue culture seeding and remarkably improving the transplanting survival rate of the catharanthus roseus tissue culture seeding.

The invention discloses a catharanthine tartrate preparation method. The catharanthine tartrate preparation method includes the steps of subjecting whole catharanthus roseus to brining, freeze drying and smashing; adding powder into water, regulating pH (potential of hydrogen) prior to extraction, and regulating pH of an extracted product to obtain a catharanthine crude product; enabling the catharanthine crude product to react with tartaric acid to salify, and crystallizing in mixed solvents to obtain catharanthine tartrate. The catharanthine tartrate preparation method has the advantages that the method is concise in step, less in consumed time and rapid in product dissolution, the catharanthine crude product high in purity is obtained directly by pH gradient separation with column chromatography separation not required, and the catharanthine tartrate which has the purity above 99% and is easy to preserve can be obtained by salifying and crystallizing; the method is simpler in purification process and capable of realizing separation and purification of catharanthine more rapidly, and the finally obtained product is high in yield and purity and can better satisfy market demand.

The present invention relates to plants of the genus Catharanthus containing a genetic resistance to Phytophthora. In particular, the present invention relates to a Catharanthus roseus plant containing a genetic resistance to the F and S isolates of P. nicotianae.