41 results about "Catharanthus" patented technology

The invention discloses a method for producing catharanthus roseus alkaloid. The method comprises the following steps: firstly, dedifferentiation culture is performed on the explant of the catharanthus roseus to get the callus of the catharanthus roseus, and through succesive transfer culture, loose and quick-growing cell line is obtained; secondly, the cell line of the callus of the catharanthus roseus obtained in the step one is processed through inducing the culture in basal culture medium provided with colchicines to get polyploidy cell line, and then expanding culture is performed to get a large amount of catharanthus roseus polyploidy cells; thirdly, the catharanthus roseus polyploidy cells are inoculated into a deffined medium to get cells being rich ofcatharanthus roseus secondary metabolites. The method of the invention produces the catharanthine with the catharanthus roseus, the industrial production of the catharanthine can be firmly realized, the production cost is low, the culture period is short, the production is not influenced by natural environment and climate, and the year-round production can be realized.

The invention belongs to the technical field of control of fruit tree diseases and particularly discloses a method for screening citrus HLB (Huanglongbing) control agents through grafting. According to the method for screening the citrus HLB (Huanglongbing) control agents, a citrus scion infected with the disease is taken as an object, the content change of Candidatus liberibacter asiaticus in the scion infected with the disease and treated with the agents is detected quantitatively through a real-time PCR (polymerase chain reaction), and the control effects of the treating agents are evaluated on the basis. The citrus scion infected with the disease is taken as a study object directly, the agent efficacy evaluation more conforms to practical situations, and influence caused by an interhost such as Diaphorina citri Kuwayama or catharanthus roseus used in screening of Candidatus liberibacter asiaticus control agents is eliminated. The problems that a screening process of the Candidatus liberibacter asiaticus control agents is complicated, a reliable and effective evaluation system is absent and the like at present are solved, and reliable technical and theoretical bases are provided for control of citrus HLB.

The invention relates to the technical field of bioengineering and discloses a method for improving camptothecin content in hairy roots of the new-type medicine source plant ophiorrhiza pumila of camptothecin. The method disclosed by the invention comprises the following steps of: cloning coding frame sequences of genes of ophiorrhiza japonica strictosidine synthase and geraniol-10-hydroxylase in catharanthus roseus to build a plant bivalent efficient expression vector containing the genes, carrying out genetic transformation on ophiorrhiza pumila via an agrobacterium rhizogenes mediated method to acquire the hairy roots of the ophiorrhiza pumila for transforming CrSTR and CrG10H genes; and inducing and treating high-yield camptothecin strains by adopting plant hormones to acquire high-yield hairy roots with the camptothecin content of 4.703mg / g DW. The MTT (Methyl Thiazolyl Tetrazolium) detection proves that camptothecin crude extract acquired via a transgenosis manner is good in biological activity and the lethality to cancer cells reaches 35.9%. By adopting the method disclosed by the invention, a new medicine source for acquiring the camptothecin is provided and a new method for producing anti-cancer medicine camptothecin in important clinical demand is provided.

The invention relates to a process for preparing vinblastine, which has simple and convenient operation and less pollution and equipment investment. The process comprises the following steps: soakingthe whole catharanthus roseus by 8-12 times of acetic acid aqueous solution with concentration of 5-20 percent for 2 hours; assisting to extract by a microwave with the extracting power of 600W and the extracting time of 2-8 minutes; filtering and adjusting the pH value of filter liquor to 8 by ammonia water; adsorbing by a macroporous absorption resin column; eluting and removing impurities by 20-60 percent of alcohol with the pH value being equal to 5; eluting by 95 percent of alcohol; concentrating eluent, adding acetone crystals, washing and drying to obtain the vinblastine. The process for preparing the vinblastine is easy to realize industrialization amplification.

The present invention relates to the field of plant secondary metabolites, particularly terpenoid indole alkaloids. More particularly the present invention relates to the production of the anti-cancer metabolites vincristine and vinblastine. The invention provides novel polynucleotide sequences derived from Catharanthus roseus and the use of said polynucleotide sequences to stimulate the production of vinblastine and / or vincristine in plants of Catharanthus roseus and plant cell lines derived thereof. The invention further relates to recombinant plants, plant cells, tissue and organ cultures obtainable by the process of the invention.

The invention relates to an extraction technology for the active ingredients of a Chinese herbal medicine catharanthus roseus, and aims at providing an efficient enzyme induction extraction method for economically and quickly obtaining a catharanthus roseus extract which is rich in four alkaloid active ingredients such as vinblastine, vincristine, catharanthine and vindoline. In allusion to the defects of long extraction time, high extraction temperature, low extraction rate and the like of the traditional extraction method, the inductive extraction method in the invention can obtain the catharanthus roseus extract which is rich in four alkaloid active ingredients by adopting the technologies such as homogenate enzyme inductive extraction, negative-pressure cavitation extraction, solvent impurity removal, enrichment refining and the like; the results show that the contents of the four alkaloid active ingredients such as vinblastine, vincristine, catharanthine and vindoline in the extract can respectively achieve 11.30-17.56%, 6.52-10.79%, 3.10-5.86% and 0.98-1.72%, which are 10-80% higher than the traditional method. The method has high extraction rate, simple equipment and safe operation, dispenses with high-temperature heating, and is suitable for the industrialized production, therefore, novel method and means are provided for the modernization of the extraction of the traditional Chinese medicine.

The invention discloses a method for disposing and improving the alkaloid content in catharanthus roseus through plant growth regulators. Catharanthus roseus seeds are put into peat vermiculite perlite culturing media to germinate and grow, after catharanthus roseus enters a flowering phase, different plant growth regulators (ethephon and methyl jasmonic acid) are mixed to form a mixture with the concentration being 0.1 mM, the mixture is sprayed on catharanthus roseus plants, the vindoline content, the catharanthine content and the vincaleukoblastinum content in the catharanthus roseus are generally improved after the processing is conducted one to two days, disposed catharanthus roseus plants are harvested, the output of alkaloids of the catharanthus roseus can be effectively improved, production cost of the catharanthus roseus is reduced, and great significance is achieved on meeting the industrialized production of anticancer alkaloids of the catharanthus roseus.

The invention relates to the technical field of biology, in particular to a method using the hypocotyl of catharanthus roseus for cultivating regenerated plants. The method comprises the following steps: placing the seeds of catharanthus roseus on an MS (Murashige and Skoog) medium for culturing, and placing the hypocotyl thereof on a callus-inducing medium for culturing until the first pair of cotyledons branches out, so as to obtain callus; inoculating the callus on a differentiation medium for culturing, so as to obtain the green callus; inoculating the green callus on an adventitious bud inducing medium for culturing, so as to obtain the adventitious bud; placing the adventitious bud on a rooting medium for culturing, so as to obtain the test-tube plantlet; and rapidly propagating thetest-tube plantlet on a rapid-propagation medium, so as to obtain the regenerated plant. By using the method of the invention, the adventitious bud differentiation rate of the hypocotyl of catharanthus roseus reaches 81% and the adventitious bud rooting rate thereof reaches 100%.

The invention relates to a method for screening a Candidatus Liberibacter resisting chemical through catharanthus roseus. The method includes the steps of cultivation of diseased catharanthus roseus plants, cutting regeneration of catharanthus roseus, optimization of cutting regeneration conditions of catharanthus roseus, chemical treatment and screening of the Candidatus Liberibacter resisting chemical. According to the method, the natural host catharanthus roseus for the plant Candidatus Liberibacter is taken as a test material for screening the Candidatus Liberibacter resisting chemical, the screening method is fast, easy and convenient to operate, and the effect of the chemical to be screened on resistance to Candidatus Liberibacter can be evaluated visually and accurately.

The invention discloses a catharanthus roseus cultivation method, relates to the technical field of plant medicinal material planting, and particularly belongs to the technical field of traditional Chinese medicinal material cultivation. The catharanthus roseus cultivation method comprises the following specific steps of cultivation land selection, control burning, land preparation, base fertilizer application, transplanting, cultivating-weeding, and harvesting-processing-storing. By adopting the catharanthus roseus cultivation method, the problems of low breeding rate, low yield and complex cultivation process during cultivation of catharanthus roseus in the prior art are solved. The catharanthus roseus breeding method has the beneficial effects that the cultivation survival rate is over 95 percent, young seedlings grow rapidly after transplanting, the yield is increased by 9-15 percent, and the process is simple.

The invention discloses a method for improving the transplanting survival rate of a catharanthus roseus tissue culture seeding. The method is characterized by comprising the following steps of: (1) selecting strongly and regularly grown catharanthus roseus aseptic test-tube seedling, opening a cover of a culture flask, adding the suitable amount of distilled water to the culture flask to immerse the root of the catharanthus roseus, and putting the catharanthus roseus seedling in an illumination incubator to culture for 3-4d to finish a seedling hardening operation; (2) taking out the catharanthus roseus aseptic test-tube seedling from the culture flask by a pair of tweezers, flushing a culture medium of the root of the catharanthus roseus aseptic test-tube seedling cleanly by running water, putting the culture medium in a test tube or a culture dish containing a water-culture nutrient solution, putting the test tube or the culture dish in the illumination incubator to continue the seedling hardening operation, and finishing the water culture when a new water root is grown up from the root of the catharanthus roseus plant after the catharanthus roseus plant is cultured for 2-7d; and (3) transplanting the newly grown catharanthus roseus water-culture plant in a transplanting medium containing vermiculite, perlite and turfy to finish the transplanting of the catharanthus roseus tissue culture seeding. The method for improving the transplanting survival rate of the catharanthus roseus tissue culture seeding disclosed by the invention has the advantages of reinforcing the antibacterial ability of the catharanthus roseus tissue culture seeding and remarkably improving the transplanting survival rate of the catharanthus roseus tissue culture seeding.

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely Catharanthus roseus, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the Catharanthus roseus; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; checking eluent until no alkaloid exists; collecting the eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and performing concentrated drying to obtain the Catharanthus roseus total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the Catharanthus roseus total alkaloid from the extracting solution of the Catharanthus roseus.

The present invention relates to the development of a unique inflorescence bearing mutant plant type lli / lli (LEAF-LESS INFLORESCENCE). Further the present invention relates to the development of a unique inflorescence bearing mutant plant type lli / lli (LEAF-LESS INFLORESCENCE) through chemical mutagenesis The present invention relates to the development of a new and distinct plant type for floricultural usage. The new type is a monogenic Mendelian recessive stable mutant plant of Catharanthus roseus with a novel leafless inflorescence architecture with increased flower frequency. This distinct plant of Catharanthus roseus was developed through chemical mutagenesis followed by salt tolerance selection.

The invention provides an externally applied traditional Chinese medicine for the treatment of lung cancer, which is prepared from main medicines and complementary medicines by blending, wherein the main medicines include 34 medicinal raw materials such as rhizoma corydalis, notoginseng, radix aucklandiae and catharanthus roseus; and the complementary medicines include flos carthami, liquorice, centipede, and Shanxi mature vinegar. The externally applied traditional Chinese medicine disclosed by the invention has a significant inhibition effect on lung cancer and is safe, effective, fast, easy to operate and economical, and hardly causes pain.

The invention relates to the technical field of bioengineering and discloses a method for improving camptothecin content in hairy roots of the new-type medicine source plant ophiorrhiza pumila of camptothecin. The method disclosed by the invention comprises the following steps of: cloning coding frame sequences of genes of ophiorrhiza japonica strictosidine synthase and geraniol-10-hydroxylase in catharanthus roseus to build a plant bivalent efficient expression vector containing the genes, carrying out genetic transformation on ophiorrhiza pumila via an agrobacterium rhizogenes mediated method to acquire the hairy roots of the ophiorrhiza pumila for transforming CrSTR and CrG10H genes; and inducing and treating high-yield camptothecin strains by adopting plant hormones to acquire high-yield hairy roots with the camptothecin content of 4.703mg / g DW. The MTT (Methyl Thiazolyl Tetrazolium) detection proves that camptothecin crude extract acquired via a transgenosis manner is good in biological activity and the lethality to cancer cells reaches 35.9%. By adopting the method disclosed by the invention, a new medicine source for acquiring the camptothecin is provided and a new method for producing anti-cancer medicine camptothecin in important clinical demand is provided.

The invention discloses a method for producing catharanthus roseus alkaloid. The method comprises the following steps: firstly, dedifferentiation culture is performed on the explant of the catharanthus roseus to get the callus of the catharanthus roseus, and through succesive transfer culture, loose and quick-growing cell line is obtained; secondly, the cell line of the callus of the catharanthusroseus obtained in the step one is processed through inducing the culture in basal culture medium provided with colchicines to get polyploidy cell line, and then expanding culture is performed to geta large amount of catharanthus roseus polyploidy cells; thirdly, the catharanthus roseus polyploidy cells are inoculated into a deffined medium to get cells being rich ofcatharanthus roseus secondarymetabolites. The method of the invention produces the catharanthine with the catharanthus roseus, the industrial production of the catharanthine can be firmly realized, the production cost is low, theculture period is short, the production is not influenced by natural environment and climate, and the year-round production can be realized.

The invention relates to a preparation method of catharanthus roseus high-content anticancer alkaloid. According to the invention, hairy roots obtained through agrobacterium rhizogenes mediation are subject to several times of subculture; an excellent clone with high growth speed and high and stable alkaloid content are screened according to the growth rate and the alkaloid content; the hairy root excellent clone is cultured by using a miniature fermenting machine (10-30L); through adjustments upon conditions such as medium components, ventilation status, illumination, temperature, exogenous hormone and culture mode, technology indexes and process flow of hairy root culturing are obtained; a hairy root clonal propagation system is established, and alkaloid is extracted, separated and purified from the hairy root.

The invention discloses a traditional Chinese medicine preparation for treating vesania and a preparation method thereof, and belongs to the field of traditional Chinese medicine. The traditional Chinese medicine preparation is prepared from the following effective ingredient raw materials: poria cocos, radix salviae miltiorrhizae, medulla junci, radix sileris, albizia flower, folium clematidis filamentosae, foldleaf daylily roots, narrow-leaved oleaster, Japanese pachysandra herb with roots, valerian, lotus petiole, herba schizonepetae, dens draconis, cholla stems, semen hyoscyami and catharanthus roseus. The traditional Chinese medicine preparation has the advantages that the selected medicine materials are well matched; the traditional Chinese medicine preparation conforms to the theory of traditional Chinese medicine and modern medicine; the effects of tranquilizing and allaying excitement, cooling the blood, lowering the pressure, clearing away the heart fire, relieving restlessness, smoothening qi, relieving water retention, relaxing the muscles and stimulating the blood circulation are achieved; the use is convenient; the absorption effect is good; no poor or toxic and side effects exist; through clinical verification, good prevention and treatment effects are achieved on the vesania; the traditional Chinese medicine preparation is applicable to prevention and treatment on the vesania.

The invention discloses a glyphosate compound aqueous agent. The glyphosate compound aqueous agent comprises the following main active components: glyphosate, a walnut leaf aqueous extract and catharanthus roseus alkaloid, and the compound aqueous agent is prepared from the following raw materials in parts by weight: 15 to 20g of glyphosate, 5 to 8g of walnut leaf water extract, 4 to 6g of catharanthus alkaloid, 1 to 2g of a solubilizer, 0.5 to 1g of a stabilizer and 500 to 600mL of water. Meanwhile, the invention further discloses a preparation method of the glyphosate compound aqueous solution and application of the glyphosate compound aqueous solution. The use amount of chemical pesticides is reduced, meanwhile, the defects that biological pesticides are slow in speed and high in use cost are overcome, crops are effectively protected from being invaded by pests, much pollution and damage to the ecological environment are avoided, the weeding spectrum is expanded, the weeding effect is improved, the weeding speed of glyphosate is increased, and the weeding composition has a good market prospect.

A method for cultivating catharanthus roseus transgenic plants induced by agrobacterium tumefacien comprises the steps as follows: taking aseptic seedling of the catharanthus roseus, and obtaining hypocotyl of the aseptic seedling; preparing bacteria solution of the agrobacterium tumefacien and utilizing the agrobacterium tumefacien for inducing transformation of the hypocotyl of catharanthus roseus; taking and placing the hypocotyl on a callus induction culture medium for culture to obtain a callus; inoculating the callus onto a differential culture medium for culture to obtain a green callus; inoculating the green callus onto an indefinite bud induction culture medium for culture to obtain an indefinite bud; and taking and placing the indefinite bud into a radication culture medium for culture to obtain the catharanthus roseus transgenic plants. In the method, a stable genetic transformation system of the catharanthus roseus induced by the agrobacterium tumefacien is established, therefore the method lays a foundation for further study on metabolic pathway control net and for the improvement of TIAs content, and can be further applied to gene engineering seed breeding for producing TIAs metabolite.

The invention belongs to the technical field of livestock feed and discloses pig feed. The pig feed is prepared from the following components in percentage by mass: 20 percent of fish meal, 20 percent of soybean meal, 10 percent of rapeseed meal, 20 percent of bran, 10 percent of corn flour, 8 percent of catharanthus roseus, 8 percent of vitamin E, 2 percent of table salts, 1 percent of citric acid, 0.5 percent of proteinase preparation and 0.5 percent of trace element additive. The pig feed disclosed by the invention also contains 8 percent of the catharanthus roseus to serve as green forage; vincaleukoblastinum is contained in the catharanthus roseus, is used as plant alkaloids, and can act in a pig body for inhibiting polymerization of microtubulin, hindering the forming of spindle microtubule and promoting hair removal of pigs at a large amount; in addition, by using 2 percent of the table salts, the salts can cause retention of water in a pig body, and the retained water influences the growth of pig hairs; the table salts simultaneously induce the accumulation of multiple oil, and aggravate the hair removal phenomenon; finally, the vincaleukoblastinum and table salt molecules interact to aggravate hair removal of the pigs, so that a large amount of hairs are removed in a feeding process of the pigs, and the amount of pig hairs is reduced; during leather production, residual pig hairs can be removed by one-time hair removal, so that the damage probability of pig skin is reduced.

The invention relates to a catharanthus roseus exosome nano-vesicle with immunocompetence and a preparation method of the catharanthus roseus exosome nano-vesicle. The Catharanthus roseus exosome nano-vesicles comprise Catharanthus roseus leaf source exosome-like nano-vesicles, Catharanthus roseus stem cambium stem cell source exosome-like nano-vesicles, Catharanthus roseus leaf callus source exosome-like nano-vesicles and Catharanthus roseus stem source exosome-like nano-vesicles. The extracted Catharanthus roseus plant-derived exosome vesicles can be maintained stable in an artificial simulated digestive system, and still exert immunoregulatory activity under strong acid, strong alkali and enzyme digestion regulation.

The invention discloses a method for improving the transplanting survival rate of a catharanthus roseus tissue culture seeding. The method is characterized by comprising the following steps of: (1) selecting strongly and regularly grown catharanthus roseus aseptic test-tube seedling, opening a cover of a culture flask, adding the suitable amount of distilled water to the culture flask to immerse the root of the catharanthus roseus, and putting the catharanthus roseus seedling in an illumination incubator to culture for 3-4d to finish a seedling hardening operation; (2) taking out the catharanthus roseus aseptic test-tube seedling from the culture flask by a pair of tweezers, flushing a culture medium of the root of the catharanthus roseus aseptic test-tube seedling cleanly by running water, putting the culture medium in a test tube or a culture dish containing a water-culture nutrient solution, putting the test tube or the culture dish in the illumination incubator to continue the seedling hardening operation, and finishing the water culture when a new water root is grown up from the root of the catharanthus roseus plant after the catharanthus roseus plant is cultured for 2-7d; and (3) transplanting the newly grown catharanthus roseus water-culture plant in a transplanting medium containing vermiculite, perlite and turfy to finish the transplanting of the catharanthus roseus tissue culture seeding. The method for improving the transplanting survival rate of the catharanthus roseus tissue culture seeding disclosed by the invention has the advantages of reinforcing the antibacterial ability of the catharanthus roseus tissue culture seeding and remarkably improving the transplanting survival rate of the catharanthus roseus tissue culture seeding.

The present invention relates to plants of the genus Catharanthus containing a broad spectrum genetic resistance to Phytophthora. In particular, the present invention relates to a Catharanthus roseus plant containing a broad spectrum genetic resistance to P. nicotianae.

The present invention relates to plants of the genus Catharanthus containing a genetic resistance to Phytophthora. In particular, the present invention relates to a Catharanthus roseus plant containing a genetic resistance to the F and S isolates of P. nicotianae.

The invention relates to a plant culture method and particularly relates to a culture method capable of improving the content of vinblastine in a catharanthus roseus plant body. H2O2, ethephon, acetylsalicylic acid and tryptophan are externally applied to catharanthus roseus seedlings or mature plants at certain concentration and sprayed for once or multiple times. branches and leaves of catharanthus roseus are harvested after being treated, the vinblastine in the branches and leaves is extracted and the content is detected, wherein the content can reach 38.2mug / g DW or over through detection, the detected highest content is 62.8mug / g DW, and the content of the vinblastine is improved in comparison with that in common untreated catharanthus roseus. The method is simple and feasible in operation, obvious in effect, the defect that the vinblastine cannot grow through tissue culture is overcome, and a new way is opened up for extraction and utilization of effective ingredients in a plant.

The invention discloses a method used for extracting high purity vincristine from cultured catharanthus roseus, and belongs to the technical field of biotechnology. The method is used for solving problems of the prior art that, catharanthus roseus is low in vinblastine content, extraction purity is lower than 98%, extraction rate is lower than 30%, industrialized production value is reduced, and huge waste is caused. According to the method, active sludge, corn stalk, and phosphoenolpyruvic acid are mixed so as to obtain a culture medium used for culturing catharanthus roseus; a mixed solution is sprayed so as to increase vincristine content of catharanthus roseus; the cultured catharanthus roseus is subjected to treatment, salt solution immersion, fermentation, and concentration so as to purify vinblastine. The method is unique; it is confirmed by detection that vinblastine content of the catharanthus roseus obtained culturing is increased by 20 to 30%, the purity of obtained vinblastine is higher than 98%, and extraction rate ranges from 55 to 60%.